scholarly journals Urinary Excretion of Tetrodotoxin Modeled in a Porcine Renal Proximal Tubule Epithelial Cell Line, LLC-PK1

Marine Drugs ◽  
2017 ◽  
Vol 15 (7) ◽  
pp. 225
Author(s):  
Takuya Matsumoto ◽  
Yui Ishizaki ◽  
Keika Mochizuki ◽  
Mitsuru Aoyagi ◽  
Yoshiharu Mitoma ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Urinary Excretion ◽  
Proximal Tubule ◽  
Cell Line ◽  
Renal Proximal Tubule ◽  
Epithelial Cell Line ◽  
Proximal Tubule Epithelial Cell

scholarly journals Kidney nanotoxicity studied in human renal proximal tubule epithelial cell line TH1

2019 ◽  
Vol 845 ◽  
pp. 403017 ◽  
Author(s):  
Monika Sramkova ◽  
Katarina Kozics ◽  
Vlasta Masanova ◽  
Iveta Uhnakova ◽  
Filip Razga ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Proximal Tubule ◽  
Cell Line ◽  
Renal Proximal Tubule ◽  
Epithelial Cell Line ◽  
Proximal Tubule Epithelial Cell

scholarly journals HK-2: An immortalized proximal tubule epithelial cell line from normal adult human kidney

1994 ◽  
Vol 45 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Michael J. Ryan ◽  
Gretchen Johnson ◽  
Judy Kirk ◽  
Sally M. Fuerstenberg ◽  
Richard A. Zager ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Proximal Tubule ◽  
Cell Line ◽  
Human Kidney ◽  
Normal Adult ◽  
Epithelial Cell Line ◽  
Adult Human ◽  
Proximal Tubule Epithelial Cell

scholarly journals Angiotensin II type 1 receptor-associated protein deficiency attenuates sirtuin1 expression in an immortalised human renal proximal tubule cell line

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Takahiro Yamaji ◽  
Akio Yamashita ◽  
Hiromichi Wakui ◽  
Kengo Azushima ◽  
Kazushi Uneda ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Protein Expression ◽  
Proximal Tubule ◽  
Cell Line ◽  
Renal Proximal Tubule ◽  
Sirtuin 1 ◽  
Epithelial Cell Line ◽  
Proximal Tubule Cell ◽  
Deficient Mice

Abstract The proximal tubule is a particularly important site for ageing-related kidney damage. Sirtuin 1 (SIRT1), an NAD+ (nicotinamide adenine dinucleotide)-dependent deacetylase in the proximal tubule, may be involved in renal injury associated with ageing. However, the mechanisms of SIRT1 regulation remain to be elucidated. We recently reported that angiotensin II type 1 receptor (AT1R)-associated protein (ATRAP)-deficient mice displayed age-associated renal function decline and tubulointerstitial fibrosis. Our data showed that SIRT1 protein expression was reduced in ATRAP-deficient mice, although the relationship between ATRAP deficiency and age-associated renal fibrosis is still not fully understood. It is, therefore, necessary to investigate how ATRAP affects SIRT1 protein expression to resolve ageing-associated kidney dysfunction. Here, since ageing studies are inherently lengthy, we used an ex vivo model of the proximal tubule to determine the role of ATRAP in SIRT1 protein expression. We first generated a clonal immortalised human renal proximal tubule epithelial cell line (ciRPTEC) expressing AT1R and ATRAP. Using this cell line, we demonstrated that ATRAP knockdown reduced SIRT1 protein expression in the ciRPTEC but did not alter SIRT1 mRNA expression. Thus, ATRAP likely mediates SIRT1 protein abundance in ciRPTEC.

scholarly journals Experimental Cell Line Models for Nephrotoxicity Screening

2021 ◽  
Vol 11 (3) ◽  
pp. 160-166
Author(s):  
I. A. Mazerkina ◽  
V. A. Evteev ◽  
A. B. Prokofiev ◽  
O. V. Muslimova ◽  
E. Yu. Demchenkova
Keyword(s):  
Epithelial Cell ◽  
Proximal Tubule ◽  
Cell Lines ◽  
Test Methods ◽  
Renal Proximal Tubule ◽  
Cell Models ◽  
Proximal Tubule Epithelial Cell ◽  
Epithelial Cell Lines

The aim of the study was to review literature data on cell models for experimental assessment of drug nephrotoxicity in vitro. Because of nephrotoxicity, 2% of new investigational medicinal products are discarded at the stage of preclinical in vivo studies in laboratory animals, and 19%—after phase 3 clinical trials. Prediction of toxicity in cell models could make drug development more cost-effective and help to reduce/avoid animal testing. At present, there are no official international guidelines for assessment of nephrotoxicity in vitro, but there is a lot of research underway. The main toxicity target in kidneys is renal proximal tubule epithelial cells, therefore the main research is focused on the development of renal proximal tubule epithelial cell lines with stable functional characteristics. Another important aspect in nephrotoxicity modeling is the choice of relevant test methods and end points which would reflect potential toxicity mechanisms. The paper reviews existing human renal proximal tubule epithelial cell lines and current test methods for assessing cytotoxicity. Promising areas for future development of cell models for nephrotoxicity assessment— are optimisation and standardisation of in vitro systems that would help to make preclinical predictions of drug nephrotoxicity in vivo.  

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

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