scholarly journals Effects of Low-Molecular-Weight Fucoidan and High Stability Fucoxanthin on Glucose Homeostasis, Lipid Metabolism, and Liver Function in a Mouse Model of Type II Diabetes

Marine Drugs ◽  
2017 ◽  
Vol 15 (4) ◽  
pp. 113 ◽  
Author(s):  
Hong-Ting Lin ◽  
Yu-Chi Tsou ◽  
Yu-Ting Chen ◽  
Wen-Jung Lu ◽  
Pai-An Hwang
Keyword(s):  
Molecular Weight ◽  
Lipid Metabolism ◽  
Liver Function ◽  
Mouse Model ◽  
Glucose Homeostasis ◽  
Type Ii Diabetes ◽  
High Stability ◽  
Low Molecular Weight ◽  
Type Ii

Parallel Reduction of Plasma Levels of High and Low Molecular Weight Kininogen in Patients with Cirrhosis

1999 ◽  
Vol 82 (11) ◽  
pp. 1428-1432 ◽  
Author(s):  
Cheryl Scott ◽  
Francesco Salerno ◽  
Elettra Lorenzano ◽  
Werner Müller-Esterl ◽  
Angelo Agostoni ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liver Disease ◽  
Liver Function ◽  
Plasma Levels ◽  
Plasma Concentrations ◽  
Normal Subjects ◽  
Low Molecular Weight ◽  
Major Band ◽  
Sds Page ◽  
Normal Controls

SummaryLittle is known about the regulation of high-molecular-weight-kininogen (HK) and low-molecular-weight-kininogen (LK) or the relationship of each to the degree of liver function impairment in patients with cirrhosis. In this study, we evaluated HK and LK quantitatively by a recently described particle concentration fluorescence immunoassay (PCFIA) and qualitatively by SDS PAGE and immunoblotting analyses in plasma from 33 patients with cirrhosis presenting various degrees of impairment of liver function. Thirty-three healthy subjects served as normal controls. Patients with cirrhosis had significantly lower plasma levels of HK (median 49 μg/ml [range 22-99 μg/ml]) and LK (58 μg/ml [15-100 μg/ml]) than normal subjects (HK 83 μg/ml [65-115 μg/ml]; LK 80 μg/ml [45-120 μg/ml]) (p < 0.0001). The plasma concentrations of HK and LK were directly related to plasma levels of cholinesterase (P < 0.0001) and albumin (P < 0.0001 and P < 0.001) and inversely to the Child-Pugh score (P < 0.0001) and to prothrombin time ratio (P < 0.0001) (reflecting the clinical and laboratory abnormalities in liver disease). Similar to normal individuals, in patients with cirrhosis, plasma HK and LK levels paralleled one another, suggesting that a coordinate regulation of those proteins persists in liver disease. SDS PAGE and immunoblotting analyses of kininogens in cirrhotic plasma showed a pattern similar to that observed in normal controls for LK (a single band at 66 kDa) with some lower molecular weight forms noted in cirrhotic plasma. A slight increase of cleavage of HK (a major band at 130 kDa and a faint but increased band at 107 kDa) was evident. The increased cleavage of HK was confirmed by the lower cleaved kininogen index (CKI), as compared to normal controls. These data suggest a defect in hepatic synthesis as well as increased destructive cleavage of both kininogens in plasma from patients with cirrhosis. The decrease of important regulatory proteins like kininogens may contribute to the imbalance in coagulation and fibrinolytic systems, which frequently occurs in cirrhotic patients.

scholarly journals Low Molecular Weight Fucoidan Can Inhibit the Fibrosis of Diabetic Kidneys by Regulating the Kidney Lipid Metabolism

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yan Wang ◽  
Yanlei Sun ◽  
Fengli Shao ◽  
Bo Zhang ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Lipid Metabolism ◽  
Kidney Disease ◽  
Western Blot ◽  
Diabetic Kidney Disease ◽  
Density Lipoprotein ◽  
Fat Deposition ◽  
Diabetic Rat ◽  
Low Molecular Weight ◽  
Diabetic Kidney

In this study, a diabetic kidney disease model was established by placing the test rats on a high-sugar/high-fat diet combined with streptozotocin induction. Histopathological examination (H&E, Masson, and PASM stain) showed pathological changes in the diabetic rat kidneys, in addition to fibrotic symptoms and collagen deposition. Immunohistochemistry and western blot analyses indicated that the diabetic condition significantly increased the expressions of fibrotic markers including collagen, α-SMA, and fibronectin. The levels of cholesterol, triglyceride, and low-density lipoprotein were also increased in diabetic kidney disease (DKD) rat blood, while the level of high-density lipoprotein was decreased. The results of Oil red O staining experiments indicated that the kidneys of diabetic rats exhibited appreciable fat deposition, with high contents of triglyceride and cholesterol. To inhibit fibrosis and reduce fat deposition, low molecular weight fucoidan (LMWF) may be used. Based on PCR and western blot analyses, LMWF can regulate the expression levels of important lipid metabolism regulators, thereby impeding the development of kidney fibrosis. Through the vitro model, it also be indicated that LMWF could inhibit fibrosis process through regulating lipid metabolism which induced by palmitic acid.

scholarly journals Central IKK2 Inhibition Ameliorates Air Pollution-Mediated Hepatic Glucose and Lipid Metabolism Dysfunction in Mice With Type II Diabetes

2018 ◽  
Vol 164 (1) ◽  
pp. 240-249 ◽  
Author(s):  
Qing Sun ◽  
Guoqing Zhang ◽  
Rucheng Chen ◽  
Ran Li ◽  
Huanhuan Wang ◽  
...  
Keyword(s):  
Air Pollution ◽  
Lipid Metabolism ◽  
Type Ii Diabetes ◽  
Type Ii ◽  
Glucose And Lipid Metabolism ◽  
Hepatic Glucose

scholarly journals Atrial Electrophysiological Remodelling in a Pre-obese Mouse Model of Type II Diabetes: Electrophysiological Mapping Studies

2012 ◽  
Vol 21 ◽  
pp. S100
Author(s):  
W. Lim ◽  
M. Neo ◽  
P. Kuklik ◽  
A. Ganesan ◽  
D. Saint ◽  
...  
Keyword(s):  
Mouse Model ◽  
Type Ii Diabetes ◽  
Obese Mouse ◽  
Type Ii
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