scholarly journals The Influence of Tetrodotoxin (TTX) on the Distribution and Chemical Coding of Caudal Mesenteric Ganglion (CaMG) Neurons Supplying the Porcine Urinary Bladder

Marine Drugs ◽  
2017 ◽  
Vol 15 (4) ◽  
pp. 101 ◽  
Author(s):  
Ewa Lepiarczyk ◽  
Agnieszka Bossowska ◽  
Jerzy Kaleczyc ◽  
Marta Majewska ◽  
Sławomir Gonkowski ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Chemical Coding ◽  
Mesenteric Ganglion ◽  
Caudal Mesenteric Ganglion

Distribution and chemical coding of sympathetic neurons in the caudal mesenteric ganglion projecting to the ovary in sexually mature gilts

2010 ◽  
Vol 58 (3) ◽  
pp. 389-403 ◽  
Author(s):  
Marlena Koszykowska ◽  
Anna Kozłowska ◽  
Joanna Wojtkiewicz ◽  
Cezary Skobowiat ◽  
Mariusz Majewski ◽  
...  
Keyword(s):  
Sympathetic Neurons ◽  
Physiological Role ◽  
Double Labelling ◽  
Nerve Fibres ◽  
Neuronal Tracing ◽  
Chemical Coding ◽  
Mesenteric Ganglion ◽  
Caudal Mesenteric Ganglion ◽  
Labelling Immunofluorescence ◽  
Sexually Mature

The distribution and co-localisation patterns of dopamine-β-hydroxylase (DβH), neuropeptide Y (NPY), somatostatin (SOM) and galanin (GAL) were investigated by use of retrograde neuronal tracing and double-labelling immunofluorescence techniques in the caudal mesenteric ganglion (CaMG) neurons supplying the ovary of adult pigs. The existence and density of nerve fibres that are immunoreactive (IR) for the above-mentioned neuroactive substances were also evaluated. Injections of a fluorescent tracer (Fast Blue; FB) into the ovaries revealed the presence of small- (76.38%) and large-sized (23.62%) FB-positive postganglionic neurons in the CaMG. Noradrenergic FB-positive cells were simultaneously NPY- (43.38%), SOM- (18.77%) and GAL- (18.31%) IR. Of the examined FB-positive neurons, 53.49% were DβH-IR but NPY-immunonegative (IN), 79.06% were DβH-IR but SOM-IN, and 77.16% were DβH-IR but GAL-IN. Small- or large-sized subsets of traced neurons were supplied by only one or a few nerve fibres, exhibiting DβH-, NPY-, SOM- and/or GAL-IR. Our data show the specific morphological as well as immunochemical structural organisation of the sympathetic neurons in the CaMG in adult gilts. The occurrence of an abundant population of noradrenergic perikarya in the CaMG may suggest their important physiological role in the regulation of gonadal function(s) in these animals.

Caudal mesenteric ganglion in the sheep – macroanatomical and immunohistochemical study

2015 ◽  
Vol 18 (2) ◽  
pp. 379-389 ◽  
Author(s):  
W. Sienkiewicz ◽  
A. Chrószcz ◽  
A. Dudek ◽  
M. Janeczek ◽  
J. Kaleczyc
Keyword(s):  
Animal Species ◽  
Immunohistochemical Study ◽  
Nerve Cells ◽  
Pelvic Cavity ◽  
Nerve Terminals ◽  
Nerve Fibres ◽  
Chemical Coding ◽  
Adult Sheep ◽  
Mesenteric Ganglion ◽  
Caudal Mesenteric Ganglion

Abstract The caudal mesenteric ganglion (CaMG) is a prevetrebral ganglion which provides innervation to a number of organs in the abdominal and pelvic cavity. The morphology of CaMG and the chemical coding of neurones in this ganglion have been described in humans and many animal species, but data on this topic in the sheep are entirely lacking. This prompted us to undertake a study to determine the localization and morphology of sheep CaMG as well as immunohistochemical properties of its neurons. The study was carried out on 8 adult sheep, weighing from 40 to 60 kg each. The sheep were deeply anaesthetised and transcardially perfused with 4% paraformaldehyde. CaMG-s were exposed and their location was determined. Macroanatomical observations have revealed that the ovine CaMG is located at the level of last two lumbar (L5 or L6) and the first sacral (S1) vertebrae. The ganglion represents an unpaired structure composed of several, sequentially arranged aggregates of neurons. Immunohistochemical investigations revealed that nearly all (99.5%) the neurons were DβH-IR and were richly supplied by VACHT-IR nerve terminals forming „basket-like” structures around the perikarya. VACHT-IR neurones were not determined. Many neurons (55%) contained immunoreactivity to NPY, some of them (10%) stained for Met-ENK and solitary nerve cells were GAL-positive. CGRP-IR nerve fibres were numerous and a large number of them simultaneously expressed immunoreactivity to SP. Single, weakly stained neurones were SP-IR and only very few nerve cells weakly stained for VIP.

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