scholarly journals 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways

Marine Drugs ◽  
2014 ◽  
Vol 12 (9) ◽  
pp. 4783-4798 ◽  
Author(s):  
Jen-Jie Lin ◽  
Jui-Hsin Su ◽  
Chi-Chu Tsai ◽  
Yi-Jen Chen ◽  
Ming-Hui Liao ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Migration ◽  
Signaling Pathways ◽  
Mtor Signaling ◽  
Human Hepatocellular Carcinoma ◽  
Migration And Invasion ◽  
Cell Migration And Invasion

scholarly journals MECP2 promotes migration and invasion of gastric cancer cells via modulating the Notch1/c-Myc/mTOR signaling pathways by suppressing FBXW7 transcription

2020 ◽  
Author(s):  
Lingyu Zhao ◽  
Xiaofei Wang ◽  
Juan Yang ◽  
Qiuyu Jiang ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Migration ◽  
Signaling Pathways ◽  
Cell Lines ◽  
Molecular Mechanisms ◽  
Mtor Signaling ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
Gene Assay

Abstract Background: Methyl-CpG-binding protein 2 (MECP2), an epigenetic regulatory factor, promotes the carcinogenesis and progression of a number of cancers. However, its role in the migration and invasion of gastric cancer (GC) as well as the underlying molecular mechanisms remain unclear.Methods: Immunohistochemistry (IHC), Western blot, and quantitative real-time PCR (qRT-PCR) were performed to measure the expressions of MECP2, FBXW7, c-Myc, mTOR and Notch1 in GC tissues and cell lines, respectively. The effects of MECP2 silencing and overexpression on GC cell migration and invasion were detected by wound healing assay and transwell assay. The mechanisms of MECP2-mediated migration and invasion were further investigated using chromatin immunoprecipitation sequencing (ChIP-Seq) and luciferase reporter gene assay.Results: In this study, we found that MECP2 facilitated the migration and invasion of GC cells. Investigation of the molecular mechanism revealed that MECP2 restrained FBXW7 transcription in GC by binding to the methylated CpG sites of FBXW7’s promoter region. MECP2 expression was remarkably negatively correlated with the FBXW7 level in GC tissues. FBXW7 expression was significantly downregulated in GC tissues and cell lines, and low FBXW7 expression was correlated with the clinicopathologic features. FBXW7 repressed cell migration and invasion by regulating the Notch1/c-Myc/mTOR signaling pathways, and knockdown of FBXW7 reversed the effects of silencing MECP2. Moreover, MECP2 upregulated the Notch1/c-Myc/mTOR signaling pathways by inhibiting FBXW7 expression at the transcription level.Conclusion: This study demonstrates that MECP2 promotes migration and invasion of GC cells via modulating the Notch1/c-Myc/mTOR signaling pathways by suppressing FBXW7 transcription. The findings suggest that MECP2 may be a novel effective therapeutic target for GC patients.

scholarly journals MicroRNA-33a-3p suppresses cell migration and invasion by directly targeting PBX3 in human hepatocellular carcinoma

Oncotarget ◽  
2016 ◽  
Vol 7 (27) ◽  
pp. 42461-42473 ◽  
Author(s):  
Shu-Yan Han ◽  
Hai-Bo Han ◽  
Xiu-Yun Tian ◽  
Hong Sun ◽  
Dong Xue ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Migration ◽  
Human Hepatocellular Carcinoma ◽  
Migration And Invasion ◽  
Cell Migration And Invasion
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