Tetroazolemycins A and B, Two New Oxazole-Thiazole Siderophores from Deep-Sea Streptomyces olivaceus FXJ8.012

Ning Liu; Fei Shang; Lijun Xi; Ying Huang

doi:10.3390/md11051524

Tetroazolemycins A and B, Two New Oxazole-Thiazole Siderophores from Deep-Sea Streptomyces olivaceus FXJ8.012

Marine Drugs ◽

10.3390/md11051524 ◽

2013 ◽

Vol 11 (5) ◽

pp. 1524-1533 ◽

Cited By ~ 18

Author(s):

Ning Liu ◽

Fei Shang ◽

Lijun Xi ◽

Ying Huang

Keyword(s):

Deep Sea ◽

Streptomyces Olivaceus

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Biosynthetic Baeyer–Villiger Chemistry Enables Access to Two Anthracene Scaffolds from a Single Gene Cluster in Deep-Sea-Derived Streptomyces olivaceus SCSIO T05

Journal of Natural Products ◽

10.1021/acs.jnatprod.8b00077 ◽

2018 ◽

Vol 81 (7) ◽

pp. 1570-1577 ◽

Cited By ~ 13

Author(s):

Chunyan Zhang ◽

Changli Sun ◽

Hongbo Huang ◽

Chun Gui ◽

Liyan Wang ◽

...

Keyword(s):

Gene Cluster ◽

Deep Sea ◽

Single Gene ◽

Streptomyces Olivaceus

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Genome Sequencing of Streptomyces olivaceus SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining

Marine Drugs ◽

10.3390/md17100593 ◽

2019 ◽

Vol 17 (10) ◽

pp. 593 ◽

Cited By ~ 3

Author(s):

Chunyan Zhang ◽

Wenjuan Ding ◽

Xiangjing Qin ◽

Jianhua Ju

Keyword(s):

Metabolic Engineering ◽

Deep Sea ◽

Genome Mining ◽

Gene Clusters ◽

Type I ◽

Biosynthetic Gene ◽

Biosynthetic Gene Clusters ◽

Genus Streptomyces ◽

Disruption Method ◽

Streptomyces Olivaceus

Marine-sourced actinomycete genus Streptomyces continues to be an important source of new natural products. Here we report the complete genome sequence of deep-sea-derived Streptomyces olivaceus SCSIO T05, harboring 37 putative biosynthetic gene clusters (BGCs). A cryptic BGC for type I polyketides was activated by metabolic engineering methods, enabling the discovery of a known compound, lobophorin CR4 (1). Genome mining yielded a putative lobophorin BGC (lbp) that missed the functional FAD-dependent oxidoreductase to generate the d-kijanose, leading to the production of lobophorin CR4 without the attachment of d-kijanose to C17-OH. Using the gene-disruption method, we confirmed that the lbp BGC accounts for lobophorin biosynthesis. We conclude that metabolic engineering and genome mining provide an effective approach to activate cryptic BGCs.

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