scholarly journals Fine Structure in Multi-Phase Zr8Ni21-Zr7Ni10-Zr2Ni7 Alloy Revealed by Transmission Electron Microscope

Materials ◽  
2015 ◽  
Vol 8 (7) ◽  
pp. 4618-4630 ◽  
Author(s):  
Haoting Shen ◽  
Leonid Bendersky ◽  
Kwo Young ◽  
Jean Nei
1992 ◽  
Vol 70 (11) ◽  
pp. 2163-2169 ◽  
Author(s):  
D. J. S. Barr ◽  
N. L. Désaulniers

The flagellar apparatuses of 14 species of Phytophthora, 2 of Halophytophthora, and 4 of Pythium are compared in the transmission electron microscope. Except for Phytophthora infestans and Phytophthora mirabilis there were no significant differences in fine structure morphology. There are six flagellar roots: a ribbed triplet consisting of three main microtubules and secondary microtubules; an anterior doublet; a multistranded, band-shaped root of five to nine microtubules; a posterior root of two to four microtubules; and roots consisting of arrays of cytoplasmic microtubules and nuclear-associated microtubules. In P. infestans and P. mirabilis the multistranded root is missing, the posterior root contains five or six microtubules, and the anterior ribbed root contains four main microtubules. The transitional zones in all species are similar. The relationship of the Pythiaceae with other Oomycetes is discussed. Key words: taxonomy, phytogeny, cytology, Oomycetes, Pythiaceae.


Author(s):  
R.D. Leapman ◽  
S.B. Andrews

Recent advances in electron energy loss spectroscopy (EELS) have significantly extended the range of applications for biological microanalysis. For example, EELS can now detect physiological concentrations of the important element calcium in rapidly frozen cells with a sensitivity greater than that achievable by energy-dispersive x-ray spectroscopy (EDXS). It can also detect small numbers of phosphorus atoms bound to macromolecular assemblies, and measure water distributions in frozen hydrated tissue. Here we discuss some of these developments in the context of detection limits and mapping techniques in the scanning transmission electron microscope (STEM) and energy-filtering transmission electron microscope (EFTEM).The useful information about elemental composition in EELS of biological specimens generally resides in weak core-edge signals corresponding to atomic concentrations in the 10−5−10−3 (1–100 mmol/kg dry weight) range. For example, the Ca L2,3 signal/background ratio is typically only 10−3 and it is necessary to measure differences in signal that are only 104 of the background. Changes in low-loss fine structure corresponding to varying chemical composition are also very subtle; for example, detection of a 3% change in water content requires reliable measurement of a 0.1 eV shift in the low-loss intensity maximum. To extract such information requires efficient parallel detection of the energy loss spectrum and a high-brightness source to provide a sufficient number of incident electrons. The dedicated STEM is particularly well-suited for analyzing low concentrations of biological elements. If desired, the probe current can be reduced into the picoampere range for low-dose, high-resolution imaging prior to elemental analysis. The STEM’S field-emission source can then be used to deliver a current approaching 10 nA into a ~10 nm diameter probe. High electron flux conditions are ideal for spectrum-imaging applications where adequate counting statistics must be achieved within a limited pixel dwell time. The cold field-emission source of the STEM has the additional advantage of providing electrons with a narrow energy spread of <0.5 eV which is important in fine structure studies.


Significant advances in knowledge of lamellae and their organization in meltcrystallized polymers have stemmed from the ability to examine internal morphologies systematically with the transmission electron microscope. Spherulites form because the first-forming (dominant) lamellae branch repetitively, often at giant screw dislocations, then diverge substantially creating a skeleton to which later-forming lamellae must accommodate. This sequence promotes chain-folding, invites fractional crystallization and modulates chemical, mechanical and thermal properties of spherulites at the inter-dominant spacing. The key feature of lamellar divergence at screw dislocations is present in individual crystals, probably deriving from pressure of uncrystallized molecular cilia; growing lamellae will also distort very substantially to gain material. If necessary, spacefilling is achieved without lamellar and crystallographic continuity by nucleating new growth at large misorientations. Individual melt-grown crystals have been studied both after extraction from a quenched matrix and in situ in thinned specimens. For polyethylene different lamellar profiles have been placed in context while their fine structure provides insights into molecular mechanisms of growth.


1996 ◽  
Vol 452 ◽  
Author(s):  
Toshimichi Ito ◽  
Takashi Ooiwa ◽  
Takanobu Nagao ◽  
Akimitsu Hatta

AbstractThe fine structure of porous silicon (PS) plated electro-chemically with indium at a low plating charge of 0.03 C/cm2 has been studied mainly using Rutherford backscattering spectrometry and a transmission electron microscope. The results obtained here show (1) that the amount of plated In atoms strongly depends on the oxidation states of the pore surfaces, (2) that the average In densities, the maximum of which is usually located near the PS - Si substrate interface, are typically larger than 1 % of the Si densities in the PS layers, and (3) that the plated In atoms are concentrated locally in pores larger in size. Two-step thermal oxidation of an In-plated PS specimen results in a structure of nm-sized Si crystallites surrounded mainly by Si oxide and In oxide.


Author(s):  
R. A. Waugh ◽  
J. R. Sommer

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.


Author(s):  
Sanford H. Vernick ◽  
Anastasios Tousimis ◽  
Victor Sprague

Recent electron microscope studies have greatly expanded our knowledge of the structure of the Microsporida, particularly of the developing and mature spore. Since these studies involved mainly sectioned material, they have revealed much internal detail of the spores but relatively little surface detail. This report concerns observations on the spore surface by means of the transmission electron microscope.


Author(s):  
K. Hama

The lateral line organs of the sea eel consist of canal and pit organs which are different in function. The former is a low frequency vibration detector whereas the latter functions as an ion receptor as well as a mechano receptor.The fine structure of the sensory epithelia of both organs were studied by means of ordinary transmission electron microscope, high voltage electron microscope and of surface scanning electron microscope.The sensory cells of the canal organ are polarized in front-caudal direction and those of the pit organ are polarized in dorso-ventral direction. The sensory epithelia of both organs have thinner surface coats compared to the surrounding ordinary epithelial cells, which have very thick fuzzy coatings on the apical surface.


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