scholarly journals Cytotoxicity Test of One-Step Self-Etching Bonding Agents by Standardized Dentin Barrier Test Using Polyurethane Discs

Materials ◽  
2013 ◽  
Vol 7 (1) ◽  
pp. 85-96 ◽  
Author(s):  
Mi-Joo Kim ◽  
Kyoung-Nam Kim ◽  
Kwang-Mahn Kim
Keyword(s):  
Cytotoxicity Test ◽  
Bonding Agents ◽  
Barrier Test ◽  
One Step ◽  
Dentin Barrier Test

Cytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells

2011 ◽  
Vol 112 (3) ◽  
pp. e83-e88 ◽  
Author(s):  
Abdülkadir Şengün ◽  
Muhammet Yalçın ◽  
Hayriye Esra Ülker ◽  
Bora Öztürk ◽  
Sema S. Hakkı
Keyword(s):  
Bonding Agents ◽  
Dentin Bonding ◽  
3 Dimensional ◽  
Dentin Bonding Agents ◽  
Barrier Test ◽  
Pulp Cells ◽  
Dentin Barrier Test

Cytotoxicity of Low pH Dentin-Bonding Agents in a Dentin Barrier Test In Vitro

2002 ◽  
Vol 28 (3) ◽  
pp. 188-192 ◽  
Author(s):  
G SCHMALZ ◽  
U SCHUSTER ◽  
A KOCH ◽  
H SCHWEIKL
Keyword(s):  
Low Ph ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Dentin Bonding Agents ◽  
Barrier Test ◽  
Dentin Barrier Test

scholarly journals 3D Printed Microfluidic Devices for Drug Release Assays

Pharmaceutics ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 13
Author(s):  
Benzion Amoyav ◽  
Yoel Goldstein ◽  
Eliana Steinberg ◽  
Ofra Benny
Keyword(s):  
3D Printing ◽  
Drug Release ◽  
Particle Production ◽  
Cost Effective ◽  
Drug Dissolution ◽  
Cytotoxicity Test ◽  
High Durability ◽  
One Step ◽  
Cost Effective Alternative ◽  
Multiple Recycling

Microfluidics research for various applications, including drug delivery, cell-based assays and biomedical research has grown exponentially. Despite this technology’s enormous potential, drawbacks include the need for multistep fabrication, typically with lithography. We present a one-step fabrication process of a microfluidic chip for drug dissolution assays based on a 3D printing technology. Doxorubicin porous and non-porous microspheres, with a mean diameter of 250µm, were fabricated using a conventional “batch” or microfluidic method, based on an optimized solid-in-oil-in-water protocol. Microspheres fabricated with microfluidics system exhibited higher encapsulation efficiency and drug content as compared with batch formulations. We determined drug release profiles of microspheres in varying pH conditions using two distinct dissolution devices that differed in their mechanical barrier structures. The release profile of the “V” shape barrier was similar to that of the dialysis sac test and differed from the “basket” barrier design. Importantly, a cytotoxicity test confirmed biocompatibility of the printed resin. Finally, the chip exhibited high durability and stability, enabling multiple recycling sessions. We show how the combination of microfluidics and 3D printing can reduce costs and time, providing an efficient platform for particle production while offering a feasible cost-effective alternative to clean-room facility polydimethylsiloxane-based chip microfabrication.

Evaluation of cytotoxicity of glass ionomer cements by dentin barrier test

2009 ◽  
Vol 25 (5) ◽  
pp. e39
Author(s):  
A. Sengun ◽  
H.E. Botsalı ◽  
M. Yalcın ◽  
F. Ozer ◽  
S.T. Tasdemır ◽  
...  
Keyword(s):  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Barrier Test ◽  
Dentin Barrier Test

scholarly journals Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hayriye Esra Ülker ◽  
Mustafa Ülker ◽  
Hasan Önder Gümüş ◽  
Muhammet Yalçın ◽  
Abdulkadir Şengün
Keyword(s):  
Cell Survival ◽  
Dental Pulp ◽  
Three Dimensional ◽  
T Antigen ◽  
Biologically Active ◽  
Luting Cements ◽  
Cytotoxicity Testing ◽  
Barrier Test ◽  
Cytotoxicity Studies ◽  
Dentin Barrier Test

This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P<0.05). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P>0.05). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.

Characterization of an in vitro dentin barrier test using a standard toxicant

1994 ◽  
Vol 20 (12) ◽  
pp. 592-594 ◽  
Author(s):  
Gottfried Schmalz ◽  
Helmut Schweikl
Keyword(s):  
Barrier Test ◽  
Dentin Barrier Test

Subsequent application of bonding agents to a one-step self-etch adhesive — Its effect with/without previous light-curing

2019 ◽  
Vol 35 (12) ◽  
pp. e299-e309 ◽  
Author(s):  
Antonin Tichy ◽  
Keiichi Hosaka ◽  
Pavel Bradna ◽  
Masaomi Ikeda ◽  
Ahmed Abdou ◽  
...  
Keyword(s):  
Bonding Agents ◽  
Subsequent Application ◽  
Light Curing ◽  
One Step ◽  
Self Etch

scholarly journals SYNTHESIS AND ANTICANCER ACTIVITY TEST OF 2-HYDROXY-N-PHENYLNICOTINAMIDE

2013 ◽  
Vol 13 (2) ◽  
pp. 166-170 ◽  
Author(s):  
Salahuddin Salahuddin ◽  
Muhammad Hanafi ◽  
Hariyanti Hariyanti
Keyword(s):  
Biologically Active ◽  
Leukemia Cells ◽  
Cytotoxicity Test ◽  
The Novel ◽  
Activity Test ◽  
Ic50 Value ◽  
Amidation Reaction ◽  
One Step ◽  
Ft Ir ◽  
The Uk

The novel compound of 2-hydroxy-N-phenylnicotinamide was synthesized from modification of the UK-3A compound, which was known biologically active to inhibit bacterial and cancer cells growth. Synthesis of this compound was carried out in one-step reaction. Analog UK-3A compound namely 2-hydroxy-N-phenylnicotinamide was synthesized from 2-hydroxynicotinic acid as the starting material using amidation reaction with aniline in presence of DCC/DMAP in pyridine. Analog UK-3A was analyzed and identified using TLC, FT-IR, LC-MS and FT-NMR spectrofotometer. The yield after purification was 42.97%. The IC50 value 85 μg/mL was obtained from cytotoxicity test against P388 Murine Leukemia cells. It indicated that the synthesis products were sufficiently potential for leukemia P388 treatment.

scholarly journals Comparing Shear Bond Strength of Two Step vs One Step B onding Agents on Ground Enamel and Dentin: An in vitro Study

2014 ◽  
Vol 3 (1) ◽  
pp. 1-3
Author(s):  
Shubhangi Mani ◽  
Darshan Deepak Shah ◽  
Narendra Manwar ◽  
Rushabh Malde
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Testing Machine ◽  
Single Bond ◽  
Universal Adhesive ◽  
Bonding Agents ◽  
Ground Enamel ◽  
Significant Difference ◽  
One Step

ABSTRACT Aim To compare the shear bond strength of Tetric N Bond (Ivoclar Vivadent) and Single Bond Universal Adhesive (3M ESPE, MN, USA) on ground enamel and dentin. Materials and methods A total of 30 extracted human maxillary and mandibular molars and premolars were used for this study and divided into two groups which were treated with Tetric N Bond (Ivoclar Vivaden) t- and Single Bond Universal Adhesive (3M ESPE, MN, USA). The shear bond strength was tested on Universal testing machine (Instron). Results There was statistically significant difference in the shear bond strength among the two groups. Conclusion Based on the results (Tetric N Bond, Ivoclar Vivadent) had higher bond strength than Single Bond Universal Adhesive (3M ESPE, MN, USA) on ground enamel and dentin. How to cite this article Shah DD, Chandak M, Manwar N, Mani S, Mani A, Saini R, Malde R. Comparing Shear Bond Strength of Two Step vs One Step Bonding Agents on Ground Enamel and Dentin: An in vitro Study. Int J Experiment Dent Sci 2014;3(1):1-3.

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