scholarly journals CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains

Materials ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6336
Author(s):  
Hina Qamar ◽  
Adil Saeed ◽  
Mohammad Owais ◽  
Touseef Hussain ◽  
Kashif Hussain ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Zeta Potential ◽  
Bacterial Resistance ◽  
Synthesis Method ◽  
Egg Yolk ◽  
Public Health Care ◽  
Beta Lactamase ◽  
One Pot ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

Worldwide, bacterial resistance to beta-lactam antibiotics is the greatest challenge in public health care. To overcome the issue, metal-based nanoparticles were extensively used as an alternative to traditional antibiotics. However, their unstable nature limits their use. In the present study a very simple, environmentally friendly, one-pot synthesis method that avoids the use of organic solvents has been proposed to design stable, novel nanocomposites. Formulation was done by mixing biogenic copper oxide (CuO) nanomaterial with glycerol and phospholipids isolated from egg yolk in an appropriate ratio at optimum conditions. Characterization was done using dynamic light scattering DLS, Zeta potential, high performance liquid chromatography (HPLC), and transmission electron microscopy (TEM). Further, its antibacterial activity was evaluated against the extended-spectrum beta-lactamase strains based on zone of inhibition and minimal inhibitory concentration (MIC) indices. Results from this study have demonstrated the formulation of stable nanocomposites with a zeta potential of 34.9 mV. TEM results indicated clear dispersed particles with an average of 59.3 ± 5 nm size. Furthermore, HPLC analysis of the egg yolk extract exhibits the presence of phospholipids in the sample and has significance in terms of stability. The newly formed nanocomposite has momentous antibacterial activity with MIC 62.5 μg/mL. The results suggest that it could be a good candidate for drug delivery in terms of bactericidal therapeutic applications.

Inhibitory Effect of Herbs on Extended Spectrum Beta Lactamase Enzyme from Escherichia coli of Healthy Broilers

2020 ◽  
Author(s):  
Arpita Shrivastav ◽  
R.K. Sharma ◽  
Neeraj Shrivastav ◽  
Vidhi Gautam ◽  
S.K. Jain
Keyword(s):  
Escherichia Coli ◽  
Bacterial Resistance ◽  
Colorimetric Method ◽  
Punica Granatum ◽  
Inhibitory Effect ◽  
Syzygium Aromaticum ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Multiplex Pcr Assay

Background: Bacterial resistance to beta-lactam antibiotics has risen dramatically in Escherichia coli from poultry and other food animals due to the production of extended-spectrum beta-lactamase enzyme (ESBLs) which degrades third generation cephalosporins.Herbs could be a better alternative for such cases. Methods: Present study was undertaken on 400 caecal samples of healthy broilers, collected from various poultry sale outlets of Jabalpur (M.P.). Samples were screened phenotypically and genotypically for the presence of extended spectrum beta lactamase producing E. coli. Inhibitory effect of fruit peel juice of Punica granatum and Syzigium aromaticum oil observed on the Beta lactamase enzyme obtained from these positive samples by colorimetric method using CENTA and NITROCEFIN as chromogenic substrate.Result: Phenotypically 135 samples/isolates were ESBL producing E.coli. On multiplex PCR assay 76 positive samples with bla TEM, blaCTX and bla SHV, gene was obtained. Oil of Syzygium aromaticum showed maximium per cent inhibition, Punica granatum depicted lower per cent inhibition with CENTA and NITROCEFIN respectively. Combination of both herbs showed increased inhibition. Tazobactum, (100µM) taken as the standard control exhibited 99.88 and 98 per cent inhibition of ESBL enzyme.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY OF NEEM LEAVES ON EXTENDED-SPECTRUM BETA-LACTAMASE AND AMBLER CLASS C COPRODUCERS FROM SKIN AND SOFT-TISSUE INFECTIONS.

2018 ◽  
Vol 11 (1) ◽  
pp. 210
Author(s):  
Pratibha J Shah ◽  
Manita T Williamson
Keyword(s):  
Antibacterial Activity ◽  
Soft Tissue ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Beta Lactamase ◽  
Soft Tissue Infections ◽  
Disc Diffusion ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Class C

 Objective: Clinical isolates coproducing extended-spectrum beta-lactamase (ESBL) and Ambler Class C (AmpC) beta-lactamase are a therapeutic challenge. The aim of the present study was to evaluate the antibacterial activity of Azadirachta indica methanolic leaf extract against lactose fermenting coproducers of ESBL and AmpC isolated from skin and soft-tissue infections (SSTIs).Methods: A total of 88 non-duplicate lactose fermenting strains isolated from SSTIs specimens were tested for their antibiotic susceptibility pattern by Kirby-Bauer disk diffusion method. Ceftazidime and cefoxitin-resistant strains were screened for coproduction of ESBL and AmpC beta-lactamase by phenotypic confirmatory disc diffusion test and E-test. Antibacterial activity of methanolic neem leaf (NLM) extract was examined by the disc diffusion method and the minimum inhibitory concentration (MIC) of NLM was determined by the agar dilution technique.Results: Thirty-seven lactose fermenting strains were confirmed to be coproducers of ESBL and AmpC, out of which 27 were Escherichia coli and 10 were Klebsiella spp. The nimbin content in the neem leaf extract was found to be 0.007% (w/w) by high-performance liquid chromatography (HPLC) analysis. The NLM extract showed inhibitory activity against all the 37 lactose fermenting isolates from SSTIs, coproducing ESBL and AmpC. The MIC of NLM extract was determined to be in the range of 4–8% (40–80 mg/ml) against the selected isolates.Conclusion: It can be stated that NLM extract might have therapeutic significance against coproducers of ESBL and AmpC beta-lactamase isolated from SSTIs.

scholarly journals Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Production in Enterobacteriaceae Isolates from Household Cats (Felis silvestris catus)

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Ana Claudia Souza Andrade ◽  
Isabela Carvalho Dos Santos ◽  
Lidiane Nunes Barbosa ◽  
Isabel Cristina da Silva Caetano ◽  
Melissa Marchi Zaniolo ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Oral Cavity ◽  
Animal Species ◽  
Bacterial Resistance ◽  
Beta Lactamase ◽  
Esbl Producer ◽  
Extended Spectrum Beta Lactamase ◽  
Anal Orifice ◽  
Extended Spectrum ◽  
Producer Strains

Background: In Brazil, cats in households has recently increased dramatically, likely due to their lower space and care requirements. We need to know the health of these companion animal species, since they have behavioral patterns that make them an important link in the epidemiological chain. Extended spectrum beta-lactamase producer strains (ESBL) are resistant to penicillin, cephalosporin and monobactam, but they are susceptible to clavulanate. The goal of this study is to detect strains of Enterobacteriaceae that produce extended spectrum beta-lactamase (ESBL) and evaluate the bacterial resistance profile in isolated cats (Felis silvestris catus) that live in a city located at west of Parana state, Brazil.Materials, Methods & Results: Swabs were aseptically collected from the anal orifice and oral cavity of 49 female domestic cats that were healthy upon clinical and physical examination, a minimum age of one year, weighing up to 3 kg, and had attended a veterinary clinic specializing in cats, in order to, later, perform the isolation and bacterial identification, antimicrobial sensibility phenotypic test and the phenotypic test to detect ESBL producer strains. From the 98 swabs collected it was possible to perform the bacterial isolation in 68 samples; 40.81% isolated from anal orifice and 28.57% isolated from oral cavity. From rectal and oral cavities 77.50% and 71.42% of the isolated were identified as Escherichia coli respectively, being 2.94% considered ESBL producer strains. In relation to bacterial resistance the antibiotics that shown more resistance in anal orifice were ampicillin, amoxicillin, nalidixic acid, sulfazotrim, tetracycline and aztreonam. In oral cavity they were ampicillin, amoxicillin, cefoxitin, amoxicillin + clavulanate, aztreonam, ceftriaxone and nalidixic acid; and the bacterial resistance index shown that 39.70% were considered high level risk.Discussion: Household cats have a very important role in society, since the benefits they provide to their owners are clear, however, it is worth pointing out that these animals also pose risks to human health, caused by the transmission of zoonoses and also the possibility of transfer of antimicrobial resistance genes between bacteria of animal and human origin, as well as between bacteria of the normal microbiota and pathogenic microorganisms of diferents origins. Therefore, it is important to understand the health of these companion animal species, because they exhibit behavioral patterns that make them an important link in the epidemiological chain of potentially infectious microorganisms, which may show antimicrobial resistance. Extended spectrum beta-lactamase producer strains (ESBL) are resistant to penicillin, cephalosporin and monobactam, but they are susceptible to clavulanate. These enzymes hydrolyze the beta-lactam ring of the antibiotic structure, inactivating them. Nowadays bacterial resistance is considered to be one of the greatest problems in public health worldwide, as infections and diseases outbraks are caused by multiresistant bacteria are more and more frequent. The results of this study demonstrate the presence of strains of Enterobacteriaceae family associated to the high bacterial resistance, with samples that indicate ESBL producer strains in domiciled cats, in a city of west Parana state in Brazil. These results confirm that these cats can be considered as reservoirs of different microbial agents and resistance gens, being a health problem by the possibility of dissemination. The cat population is multiplying in a higher proportion compared to dogs and may probably became predominant in less than one decade. Due this situation and thinking about human, animal and environmental health new phenotypic studies to confirm the resistance gens and ESBL producers should be conducted in this specie.

scholarly journals The Pattern of Organism Causing Urinary Tract Infection in Diabetic and Non Diabetic Patients in Bangladesh

1970 ◽  
Vol 4 (1) ◽  
pp. 6-8 ◽  
Author(s):  
Md Hamza Saber ◽  
Lovely Barai ◽  
J Ashraful Haq ◽  
Md Shariful Alam Jilani ◽  
Jaheda Begum
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Diabetic Patient ◽  
Bacterial Resistance ◽  
Diabetic Patients ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Sensitivity Pattern

Urinary tract infection (UTI) is common both in the diabetic and non-diabetic patients. The widespread use of antimicrobial agents leads to emergence of resistant microorganisms. Since the pattern of bacterial resistance is constantly changing, the monitoring of the antimicrobial susceptibilities of the predominantly isolated organisms becomes more important. Aim of this study was to determine the etiologic agents and their antibiotic sensitivity pattern in both diabetic and non-diabetic patients with urinary tract infection (UTI). A total of 288 diabetics (196 female and 92 males) and 63 non diabetic patients (43 female and 20 males) with symptomatic UTI were included in this study. Among the study cases, 43.8% diabetic patients and 42.9% non-diabetic patients had positive growth from urine. Rate of isolation of Escherichia coli in diabetic was less (61.8%) compared to non diabetic (77.8%). Rate of other organisms isolated in diabetic and non diabetic patients were respectively: Klebsiella sp 6.9% vs 3.7%, Enterococcus 12.2% vs3.7%, Pseudomonas species 3.8% vs 0%, Candida species 4.6% versus 3.7%, Staphylococcus aureus 4.6% versus 7.4% etc. E coli isolated from diabetic patient was significantly (p<0.05) less sensitive to ceftriaxone, ceftazidime, cefuroxime, netilmicin, gentamicin, ciprofloxacin and nitrofurantoin than that of non diabetic patients. In addition, isolation rate of the Extended Spectrum Beta Lactamase producing gram negative bacilli was found higher among diabetic population (47.8%) compared to the non-diabetics (9.1%). Key words: Urinary tract infection (UTI), Diabetic and non diabetic patient, Extended Spectrum Beta Lactamase. DOI: http://dx.doi.org/10.3329/bjmm.v4i1.8461 BJMM 2011; 4(1): 6-8

scholarly journals Silver nanoparticle Bacteriostatic activity Assessment against Extended-Spectrum Beta-Lactamase Producing Uropathogenic Klebsiella pneumonia

2020 ◽  
Vol 7 (2) ◽  
pp. 288-304
Author(s):  
Payman Akram Hamasaeed ◽  
Shayma Jabar Hyder
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Clinical Laboratory ◽  
National Committee ◽  
Klebsiella Pneumonia ◽  
Beta Lactamase ◽  
Esbl Producer ◽  
Klebsiella Species ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

The aims: Screen for the Extended-Spectrum Beta-Lactamase producing Klebsiella pneumonia from urinary tract infection UTI patients phenotypically and molecularly, also testing different sizes of silver nanoparticles as antibacterial for the resistance isolates. Methodology: Vitek Extended-Spectrum Beta-Lactamase test , molecular confirmed ESBL-producing isolates were checked by PCR for the presence of AmpC and blaCTX-M genes. Flat well microtiter plates were used for packing of antibacterial activity of silver nanoparticles . This study included 85 urine samples were collected from patients that admitted to the "Rizgary Hospital" in Erbil province from 15 June to 15 December 2018. Antibiotic sensitivity test of all isolates to (Ampicillin, Ampicillin/sulbactam Cefazolin, Ceftazidime Ceftriaxone, Cefepime, Imipenem, Nafcillin and Nitrofurantoin )antibiotics were tested by the disc-agar method as standardized by the National Committee for Clinical laboratory Standards (2006).Results: Out of 51 tested isolates of Klebsiella pneumonia, 11 (21.6%) were Extended-Spectrum Beta-Lactamase Producers. Their antibiotic resistance profile showed the rate of resistance isolates was (100%) to Ampicillin, Cefazolin, Ceftazidime and Ceftriaxone and ( 90%) to Cefepime .In addition the susceptibility to Imipenem was (72.7%) of isolates. The bands of the AmpC and bla CTX-M genes was noted in (42.9%) samples and the remain samples (57.1 %)were negative for both genes. The Minimum Inhibitory Concentration during incubation of Non ESBL producer - ESBL producer isolates in series concentrations of silver nanoparticles size (20 nm) was between (625 -2500) µg/ml and for size (90 nm), it was (1250) µg/ml for non ESBL producer and not affected ESBL producer isolates .The increased percentage rates of β-lactamase producing Klebsiella species were seen which was considered as an alarm, due to limitation in treatment options for UTI. It was appeared that Imipenem currently is the main available antibiotic for UTI treatment as a drug of choice. It was noticed that different sizes of silver nanoparticles showed antibacterial activity for ESBL producing isolates.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY OF NEEM LEAVES ON EXTENDED-SPECTRUM BETA-LACTAMASE AND AMBLER CLASS C COPRODUCERS FROM SKIN AND SOFT-TISSUE INFECTIONS.

2018 ◽  
Vol 11 (1) ◽  
pp. 210
Author(s):  
Pratibha J Shah ◽  
Manita T Williamson
Keyword(s):  
Antibacterial Activity ◽  
Soft Tissue ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Beta Lactamase ◽  
Soft Tissue Infections ◽  
Disc Diffusion ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Class C

 Objective: Clinical isolates coproducing extended-spectrum beta-lactamase (ESBL) and Ambler Class C (AmpC) beta-lactamase are a therapeutic challenge. The aim of the present study was to evaluate the antibacterial activity of Azadirachta indica methanolic leaf extract against lactose fermenting coproducers of ESBL and AmpC isolated from skin and soft-tissue infections (SSTIs).Methods: A total of 88 non-duplicate lactose fermenting strains isolated from SSTIs specimens were tested for their antibiotic susceptibility pattern by Kirby-Bauer disk diffusion method. Ceftazidime and cefoxitin-resistant strains were screened for coproduction of ESBL and AmpC beta-lactamase by phenotypic confirmatory disc diffusion test and E-test. Antibacterial activity of methanolic neem leaf (NLM) extract was examined by the disc diffusion method and the minimum inhibitory concentration (MIC) of NLM was determined by the agar dilution technique.Results: Thirty-seven lactose fermenting strains were confirmed to be coproducers of ESBL and AmpC, out of which 27 were Escherichia coli and 10 were Klebsiella spp. The nimbin content in the neem leaf extract was found to be 0.007% (w/w) by high-performance liquid chromatography (HPLC) analysis. The NLM extract showed inhibitory activity against all the 37 lactose fermenting isolates from SSTIs, coproducing ESBL and AmpC. The MIC of NLM extract was determined to be in the range of 4–8% (40–80 mg/ml) against the selected isolates.Conclusion: It can be stated that NLM extract might have therapeutic significance against coproducers of ESBL and AmpC beta-lactamase isolated from SSTIs.

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