scholarly journals 3D Bioprinting of Biosynthetic Nanocellulose-Filled GelMA Inks Highly Reliable for Soft Tissue-Oriented Constructs

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 4891
Author(s):  
Alexandra I. Cernencu ◽  
Adriana Lungu ◽  
Diana M. Dragusin ◽  
Izabela C. Stancu ◽  
Sorina Dinescu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Soft Tissue ◽  
Cellulose Nanofibrils ◽  
High Viscosity ◽  
Biological Properties ◽  
Adipose Stem Cells ◽  
Oxidized Cellulose ◽  
3D Bioprinting ◽  
High Viability ◽  
Human Adipose Stem Cells

Bioink-formulations based on gelatin methacrylate combined with oxidized cellulose nanofibrils are employed in the present study. The parallel investigation of the printing performance, morphological, swelling, and biological properties of the newly developed hydrogels was performed, with inks prepared using methacrylamide-modified gelatins of fish or bovine origin. Scaffolds with versatile and well-defined internal structure and high shape fidelity were successfully printed due to the high viscosity and shear-thinning behavior of formulated inks and then photo-crosslinked. The biocompatibility of 3D-scaffolds was surveyed using human adipose stem cells (hASCs) and high viability and proliferation rates were obtained when in contact with the biomaterial. Furthermore, bioprinting tests were performed with hASCs embedded in the developed formulations. The results demonstrated that the designed inks are a versatile toolkit for 3D bioprinting and further show the benefits of using fish-derived gelatin for biofabrication.

scholarly journals Hybrid Adipogenic Implants from Adipose Stem Cells for Soft Tissue Reconstruction In Vivo

2010 ◽  
Vol 16 (11) ◽  
pp. 3299-3307 ◽  
Author(s):  
Eduardo K. Moioli ◽  
Mo Chen ◽  
Rujing Yang ◽  
Bhranti Shah ◽  
June Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Soft Tissue ◽  
Adipose Stem Cells ◽  
Soft Tissue Reconstruction ◽  
Tissue Reconstruction

scholarly journals Porous Cellulose-Collagen Scaffolds For Soft Tissue Regeneration: Influence of Cellulose Derivatives On Mechanical Properties And Compatibility With Adipose-Derived Stem Cells.

2022 ◽  
Author(s):  
Katarína Kacvinská ◽  
Martina Trávničková ◽  
Lucy Vojtová ◽  
Petr Poláček ◽  
Jana Dorazilová ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Adhesion ◽  
Soft Tissue ◽  
Vascular Tissue ◽  
Biological Properties ◽  
Cellulose Derivatives ◽  
Adipose Derived Stem Cells ◽  
Soft Tissue Regeneration

Abstract This study deals with cellulose derivatives in relation to the collagen fibrils in composite collagen-cellulose scaffolds for soft tissue engineering. Two types of cellulose, i.e., oxidized cellulose (OC) and carboxymethyl cellulose (CMC), were blended with collagen (Col) to enhance its elasticity, stability and sorptive biological properties, e.g. hemostatic and antibacterial features. The addition of OC supported the resistivity of the Col fibrils in a dry environment, while in a moist environment OC caused a radical drop. The addition of CMC reduced the mechanical strength of the Col fibrils in both environments. The elongation of the Col fibrils was increased by both types of cellulose derivatives in both environments, which is closely related to tissue like behaviour. In these various mechanical environments, the ability of human adipose-derived stem cells (hADSCs) to adhere and proliferate was significantly greater in the Col and Col/OC scaffolds than in the Col/CMC scaffold. This is explained by deficient mechanical support and loss of stiffness due to the high swelling capacity of CMC. Although Col/OC and Col/CMC acted differently in terms of mechanical properties, both materials were observed to be cytocompatible, with varying degrees of further support for cell adhesion and proliferation. While Col/OC can serve as a scaffolding material for vascular tissue engineering and for skin tissue engineering, Col/CMC seems to be more suitable for moist wound healing, e.g. as a mucoadhesive gel for exudate removal, since there was almost no cell adhesion.

Generic method of printing window adjustment for extrusion-based 3D-bioprinting to maintain high viability of mesenchymal stem cells in an alginate-gelatin hydrogel

Bioprinting ◽  
2020 ◽  
Vol 20 ◽  
pp. e00094 ◽  
Author(s):  
Fritz Koch ◽  
Kevin Tröndle ◽  
Günter Finkenzeller ◽  
Roland Zengerle ◽  
Stefan Zimmermann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
3D Bioprinting ◽  
Gelatin Hydrogel ◽  
High Viability

scholarly journals Modulation of Human Adipose Stem Cells’ Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1939
Author(s):  
Katharina M. Prautsch ◽  
Alexander Schmidt ◽  
Viola Paradiso ◽  
Dirk J. Schaefer ◽  
Raphael Guzman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Neurotrophic Factor ◽  
Axonal Growth ◽  
Cellular Level ◽  
Adipose Stem Cells ◽  
Transcriptional Analysis ◽  
Axonal Outgrowth ◽  
Human Adipose Stem Cells

We report on a potential strategy involving the exogenous neurotrophic factors (NTF) for enhancing the neurotrophic capacity of human adipose stem cells (ASC) in vitro. For this, ASC were stimulated for three days using NTF, i.e., nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT3), NT4, glial cell-derived neurotrophic factor (GDNF), and ciliary neurotrophic factor (CNTF). The resulting conditioned medium (CM) as well as individual NTF exhibited distinct effects on axonal outgrowth from dorsal root ganglion (DRG) explants. In particular, CM derived from NT3-stimulated ASC (CM-NT3-ASC) promoted robust axonal outgrowth. Subsequent transcriptional analysis of DRG cultures in response to CM-NT3-ASC displayed significant upregulation of STAT-3 and GAP-43. In addition, phosphoproteomic analysis of NT3-stimulated ASC revealed significant changes in the phosphorylation state of different proteins that are involved in cytokine release, growth factors signaling, stem cell maintenance, and differentiation. Furthermore, DRG cultures treated with CM-NT3-ASC exhibited significant changes in the phosphorylation levels of proteins involved in tubulin and actin cytoskeletal pathways, which are crucial for axonal growth and elongation. Thus, the results obtained at the transcriptional, proteomic, and cellular level reveal significant changes in the neurotrophic capacity of ASC following NT3 stimulation and provide new options for improving the axonal growth-promoting potential of ASC in vitro.

scholarly journals Strategies for the hypothermic preservation of cell sheets of human adipose stem cells

PLoS ONE ◽  
2019 ◽  
Vol 14 (10) ◽  
pp. e0222597 ◽  
Author(s):  
Sara Freitas-Ribeiro ◽  
Andreia Filipa Carvalho ◽  
Marina Costa ◽  
Mariana Teixeira Cerqueira ◽  
Alexandra Pinto Marques ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Cell Sheets ◽  
Hypothermic Preservation ◽  
Human Adipose Stem Cells

Exogenously added BMP-6, BMP-7 and VEGF may not enhance the osteogenic differentiation of human adipose stem cells

2013 ◽  
Vol 31 (5) ◽  
pp. 141-153 ◽  
Author(s):  
Laura Kyllönen ◽  
Suvi Haimi ◽  
Janne Säkkinen ◽  
Hannu Kuokkanen ◽  
Bettina Mannerström ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Adipose Stem Cells ◽  
Human Adipose Stem Cells

scholarly journals The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0202740 ◽  
Author(s):  
Miina Ojansivu ◽  
Ayush Mishra ◽  
Sari Vanhatupa ◽  
Miia Juntunen ◽  
Antonina Larionova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Borosilicate Glasses ◽  
Glass Dissolution ◽  
Human Adipose Stem Cells
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