scholarly journals Effects of Different Chenopodium formosanum Parts on Antioxidant Capacity and Optimal Extraction Analysis by Taguchi Method

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4679
Author(s):  
Chin-Tung Wu ◽  
Wei-Hsun Wang ◽  
Wen-Shin Lin ◽  
Shiou-Yih Hu ◽  
Cheng-You Chen ◽  
...  
Keyword(s):  
Taguchi Method ◽  
Agricultural Waste ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Waste Reduction ◽  
Total Phenol Content ◽  
Native Plant ◽  
Flavonoid Content ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Chenopodium formosanum (CF), rich in nutrients and antioxidants, is a native plant in Taiwan. During the harvest, the seeds are collected, while the roots, stems, and leaves remain on the field as agricultural waste. In this study, di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) radical scavenging ability and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability experiments of seeds, leaves, stems, and roots were designed using the Taguchi method (TM) under three conditions: Ethanol concentration (0–100%), temperature (25–65 °C), and extraction time (30–150 min). The result demonstrates that seeds and leaves have higher radical scavenging ability than stems and roots. Many studies focused on CF seeds. Therefore, this study selected CF leaves and optimized DPPH, ABTS, total phenol content (TPC), total flavonoid content (TFC), and reducing power (RP) through TM, showing that the predicted value of the leaf is close to the actual value. The optimized results of CF leaves were DPPH 85.22%, ABTS 46.51%, TPC 116.54 µg GAE/mL, TFC 143.46 µg QE/mL, and RP 23.29 µg VCE (vitamin C equivalent)/mL. The DPPH and ABTS of CF leaves were second only to the results of CF seeds. It can be seen that CF leaves have the potential as a source of antioxidants and help in waste reduction.

scholarly journals Functionality of Silk Cocoon (Bombyx mori L.) Sericin Extracts Obtained through High-Temperature Hydrothermal Method

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5314
Author(s):  
Wei-Hsun Wang ◽  
Wen-Shin Lin ◽  
Chia-Hung Shih ◽  
Cheng-You Chen ◽  
Siao-Hong Kuo ◽  
...  
Keyword(s):  
Vitamin C ◽  
Hydrothermal Method ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenol Content ◽  
Tyrosinase Inhibition ◽  
Flavonoid Content ◽  
Silk Cocoon ◽  
Radical Scavenging Ability

Sericin, a textile waste, can be used for antioxidant and skin-whitening purposes. The hydrothermal method of extracting sericin is more eco-friendly than are chemical and enzymatic methods. In this study, silk cocoons were cut into pieces and then subjected to hydrothermal extraction at three temperatures (160, 200, and 220 °C) to obtain sericin extracts (Sericin160, Sericin200, and Sericin220, respectively). Antioxidant activity and tyrosinase inhibition were measured to determine the extracts’ effectiveness. Sericin220 was the strongest antioxidant, with total phenol content, total flavonoid content, and ferric reducing power of 62.19 ± 0.04 mg gallic acid equivalents/g dry weight, 0.07 ± 0.01 mg quercetin equivalent/g dry weight, and 181.49 ± 0.024 mg vitamin C equivalent/g dry weight, respectively. The half-maximal inhibitory concentrations for DPPH and ABTS free-radical scavenging ability were 6.41 ± 0.05 and 0.79 ± 0.37 mg/mL, respectively. Sericin220 also exhibited the highest tyrosinase inhibition activity (70.82 ± 4.1 mg vitamin C equivalent/g), indicating its whitening potential.

scholarly journals In VitroAntioxidant Properties of Three Varieties ofAllium sativumL. Extracts

2010 ◽  
Vol 7 (s1) ◽  
pp. S573-S579 ◽  
Author(s):  
R. T. Narendhirakannan ◽  
K. Rajeswari
Keyword(s):  
Free Radical ◽  
Allium Sativum ◽  
Preventive Intervention ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Garlic Bulb ◽  
Total Antioxidant ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Many herbs possess antioxidant ingredients that provide efficacy by additive or synergistic activities.Allium sativumL. is a strong astringent, used for the treatment of liver and spleen diseases, rheumatism and tumors. The antioxidant activities of different concentrations of ethanolic extracts of garlic bulb of three varieties were determined by the four assaysi.e. DPPH radical scavenging assay, reducing power ability, hydrogen peroxide scavenging assay and total antioxidant capacity. Due to its natural origin and potent free-radical scavenging ability,Allium sativumL. could be used as a potential preventive intervention for free radical-mediated diseases.

Antioxidant Activities of Crude Extract and Chromatographic Fraction from Hsian-Tsao (Mesona Procumbens Hemsl.)

2012 ◽  
Vol 550-553 ◽  
pp. 1784-1789 ◽  
Author(s):  
Ting Qiu ◽  
Bi Yu Wang ◽  
Xiao Cui Lin ◽  
Yan Xiang Wu
Keyword(s):  
Lipid Peroxidation ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Dpph Radical ◽  
Chelating Ability ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

This study aimed at evaluating the antioxidative activity of crude hsian-tsao extracted by acetone and the Fraction E purified by AB-8 macroporous resin chromatography column. The antioxidative activities, including the DPPH radical-scavenging effects, Fe2+-chelating ability, and reducing power as well as the inhibition of lipid peroxidation, were studied. Results demonstrated that both crude extract and Fraction E were more effective in the inhibition of lipid peroxidation and the DPPH radical-scavenging ability than that in the Fe2+-chelating ability. Based on the inhibition of lipid peroxidation, the order of the antioxidant activities was follows: Fraction E >GA> crude extract >Vc. Fraction E seemed to be a promising product of natural antioxidants enriched with phenolic compounds

Differential Antioxidant Composition and Potential of some commonly used Indian Spices

2017 ◽  
Vol 4 (03) ◽  
Author(s):  
SHARDA CHOUDHARY ◽  
ANDY PEREIRA ◽  
SUPRATIM BASU ◽  
A. K. VERMA
Keyword(s):  
Antioxidant Capacity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Food Preservatives ◽  
Nitric Oxide Radical ◽  
Radical Scavenging Ability ◽  
Phytochemical Components

Spices and herbs have been added to Indian foods not only as flavoring agents but also as natural supplements of medicines and food preservatives. The purpose of this study was to study the composition and antioxidant potential of some commonly used seed spices and derive a correlation between their phytochemical components and antioxidant capacity. Crushed seeds of cumin (Cuminumcyminum), coriander (Coriandrumsativum), fennel (Foeniculumvulgare), fenugreek (Trigonellafoenum-graecum), ajwain (Trachyspermumammi) and turmeric (Curcuma longa)were usedto prepare methanolic extract and iron(III) reduction, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, hydrogen peroxide , superoxide and nitric oxide radical scavenging, reducing power were assayed as an index of the antioxidant capacity along with phenolics and flavonoids were also estimated. In addition to turmeric, ajwain, fennel and coriander showed higher phenolic, flavonoid content, and antioxidant potential in comparison to fenugreek and cumin. The extracts of these spices are promising sources of alternative medicine with high free radical scavenging ability OF synthetic antioxidants and can also be used for therapeutic purposes as well.

scholarly journals Cannabinoids accumulation in hemp (Cannabis sativa L) plants under LED light spectra and their discrete role as a stress marker

2020 ◽  
Author(s):  
Md. Jahirul Islam ◽  
Md Obyedul Kalam Azad ◽  
Byung Ryeol Ryu ◽  
Md Hafizur Rahman ◽  
Jung Dae Lim ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Light Treatment ◽  
Total Phenol Content ◽  
Oxygen Species ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Light Spectra ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Dpph Radical Scavenging

Abstract Hemp adaptability through physiological and biochemical changes was studied under 10 LED light spectra and natural light in a controlled aeroponic system. Light treatments were imposed on 25 days aged seedlings for 16 hours daily (300 µmol m-2s-1) for 20 days. Plant accumulated highest Cannabidiol (CBD) in R7:B2:G1 light treatment, with relatively higher photosynthetic rate and lower reactive oxygen species, total phenol content, total flavonoid content, DPPH radical scavenging capacity, and antioxidant enzymatic activities. Tetrahydrocannabinol (THC) also accumulated higher in white, R8:B2, and R7:B2:G1 light with less evidence of stress modulated substances. These results indicated that CBD and THC have no or little relation with light-mediated abiotic stress in hemp plants. On the contrary, Tetrahydrocannabinolic acid (THCA) was accumulated higher in R6:B2:G1:FR1 and R5:B2:W2:FR1 light treatment along with lower photosynthetic rate and higher reactive oxygen species, total phenol content, total flavonoid content, DPPH radical scavenging capacity, and antioxidant enzymatic activities. However, Cannabidiolic acid (CBDA) was accumulated higher in R6:B2:G1:FR1 light treatment with higher stress modulated substances and lower physiological traits. CBDA was also accumulated higher in R8:B2 and R7:B2:G1 light treatments with less evidence of stress modulated substances. Besides, Greenlight influenced in CBD and CBDA synthesis where FR and UV-A (along with green) play a positive and negative role in this process, respectively. These results indicate that the role of THCA as a stress marker is more decisive in hemp plant than other cannabinoids under attributed light-mediated stress.

scholarly journals Phytochemical and Antioxidant Properties Of Traditionally Used Mistletoes In Nepal

2021 ◽  
Vol 14 (14) ◽  
pp. 83-89
Author(s):  
Kranti Kumal ◽  
Deepak Raj Pant ◽  
Biva Aryal ◽  
Giri Raj Tripathi ◽  
Giri Prasad Joshi
Keyword(s):  
Phenolic Content ◽  
Scientific Evidence ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Viscum Album ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Phytochemical Constituents ◽  
Dpph Radical Scavenging

Mistletoes are being used for food or beverage and for variety of medicinal purposes. In Nepal, mistletoes are traditionally used as fodder and as remedy to cure various ailments. In this study, methnolic extracts of seven common mistletoe species (Helixanthera ligustrina, Macrosolen cochinchinensis, Scurrula elata, S. parasitica, Viscum album, V. articulatum and V. liquidambaricola) were analyzed for their phytochemical constituents and antioxidant potential. The total flavonoid content (TFC) and total phenol content (TPC) were quantitatively determined by aluminum chloride colorimetric and folin-cioalteau reagent methods, respectively. The TFC was found highest for V. album (31.5 ± 2.3 mg QE/g) and lowest for M. cochinchinensis (24.9 ± 2.3 mg QE/g). Similarly, highest total phenolic content was found in S. parasitica (32.9 ± 2.5 mg GAE/g) and lowest in V. album (20.6 ± 2.1 mg GAE/g). The antioxidant activity measured by DPPH radical scavenging assay was found best for S. parasitica (with lowest IC₅₀ value; 26.0 ± 0.7 µg/ml) among the studied mistletoes. Overall analysis provides scientific evidence in favor of indigenous use of these mistletoes as a source of fodder, food and for medicinal purposes

Anticholinesterase and Antioxidant Activities of Spilanthes filicaulis Whole Plant Extracts for the Management of Alzheimer’s Disease

2019 ◽  
Vol 15 (2) ◽  
pp. 103-113
Author(s):  
Taiwo O. Elufioye ◽  
Cynthia C. Unachukwu ◽  
Adebola O. Oyedeji
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Whole Plant ◽  
Butyryl Cholinesterase ◽  
Dpph Radical Scavenging

Background: Spilanthes filicaulis is a tropical herb implicated as a memory enhancer in ethnomedicine. Objective: The study investigated acetyl/butyryl cholinesterase inhibitory and antioxidant activities of different extracts of S. filicaulis whole plant and correlated them to its phytochemical constituents. Methods: The powdered whole plant was successively extracted with n-hexane, ethyl acetate and methanol. Acetyl cholinesterase (AChE) and Butyryl cholinesterase (BuChE) inhibitory activity were evaluated by Ellman colorimetry assay. Antioxidant activity was tested using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing power and nitric oxide scavenging assays. Total phenolic, flavonoid and tannin were estimated using standard methods. Correlation was determined using Quest Graph™ Regression Calculator. Results: Various extracts exhibited concentration-dependent AChE and BuChE inhibitory activity with ethyl acetate extract being the highest with IC50 of 0.77 μg/mL and 0.92 μg/mL for AChE and BuChE respectively. The ethyl acetate extract also showed the highest reducing power when compared with the other extracts. The methanol extract had slightly higher phenolic and flavonoid content and showed the highest DPPH radical scavenging effect. DPPH scavenging, AChE and BuChE inhibition had high correlation with the total flavonoid content with R2 values of 1.00, 0.800 and 0.992 respectively while nitric oxide scavenging had high correlation with phenolics and tannins with R2 = 0.942 and 0.806 respectively. Conclusion: These results show that the extracts of the whole plant of S. filicaulis possess significant AChE/BuChE inhibitory and antioxidant properties, mostly due to its flavonoid content, suggesting the possible use of the plant in neurodegenerative diseases such as AD.

Production and Preliminary Characterization of Antioxidant Polysaccharide by Submerged Culture of Culinary and Medicinal Fungi Cordyceps militaris CICC14013

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Wan Chen ◽  
Gaoqiang Liu ◽  
Huandong Yang ◽  
Zhichao Wu ◽  
Hailong Yang
Keyword(s):  
Submerged Culture ◽  
Biological Activities ◽  
Average Molecular Weight ◽  
Radical Scavenging ◽  
Deae Cellulose ◽  
Reducing Power ◽  
Cordyceps Militaris ◽  
Dpph Radical ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Abstract Polysaccharides from Cordyceps militaris show multiple biological activities, such as antioxidation, antitumor, antivirus and immunomodulating properties, etc. The nutritional requirements for antioxidant polysaccharide bio-synthesis by submerged culture of C. militaris were evaluated on the basis of assay for DPPH radical scavenging ability and reducing power in this work. The results showed the best antioxidant activity of polysaccharide could be achieved when the media consisted of mannose, peptone, MgSO4, Na2HPO4, and KH2PO4. Furthermore, the main fraction of antioxidant polysaccharide, CMP-2, was preliminary isolated from the fermentation broth of C. militaris by ethanol precipitation, filtration, and DEAE cellulose-52 chromatography. CMP-2 was composed of mannose, rhamnose, glucose and galactose in a ratio of 2527:164:172:100, and had an average molecular weight (Mw) of approximately 8175 Da. The results suggested antioxidant polysaccharide could be produced by submerged culture of C. militaris and the medium composition significantly affected its DPPH radical scavenging ability and reducing power.

scholarly journals Cannabinoids Accumulation in Hemp (Cannabis sativa L.) Plants under LED Light Spectra and Their Discrete Role as a Stress Marker

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 710
Author(s):  
Md. Jahirul Islam ◽  
Byeong Ryeol Ryu ◽  
Md. Obyedul Kalam Azad ◽  
Md. Hafizur Rahman ◽  
Eun Ju Cheong ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Light Treatment ◽  
Total Phenol Content ◽  
Oxygen Species ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Light Spectra ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Dpph Radical Scavenging

Hemp adaptability through physiological and biochemical changes was studied under 10 LED light spectra and natural light in a controlled aeroponic system. Light treatments were imposed on 25 days aged seedlings for 16 h daily (300 µmol m−2 s−1) for 20 days. Plant accumulated highest Cannabidiol (CBD) in R7:B2:G1 light treatment, with relatively higher photosynthetic rate and lower reactive oxygen species, total phenol content, total flavonoid content, DPPH radical scavenging capacity, and antioxidant enzymatic activities. Tetrahydrocannabinol (THC) also accumulated at a higher level in white, R8:B2, and R7:B2:G1 light with less evidence of stress-modulated substances. These results indicated that CBD and THC have no or little relation with light-mediated abiotic stress in hemp plants. On the contrary, Tetrahydrocannabinolic acid (THCA) was accumulated higher in R6:B2:G1:FR1 and R5:B2:W2:FR1 light treatment along with lower photosynthetic rate and higher reactive oxygen species, total phenol content, total flavonoid content, DPPH radical scavenging capacity, and antioxidant enzymatic activities. However, Cannabidiolic acid (CBDA) was accumulated higher in R6:B2:G1:FR1 light treatment with higher stress-modulated substances and lower physiological traits. CBDA was also accumulated higher in R8:B2 and R7:B2:G1 light treatments with less evidence of stress-modulated substances. Besides, Greenlight influenced CBD and CBDA synthesis where FR and UV-A (along with green) play a positive and negative role in this process. Overall, the results indicated that the treatment R7:B2:G1 enhanced the medicinal cannabinoids most, and the role of THCA as a stress marker is more decisive in the hemp plant than in other cannabinoids under attributed light-mediated stress.

scholarly journals Antioxidative potential of Chenopodium botrys L. (Amaranthaceae)

2015 ◽  
Vol 61 (2) ◽  
pp. 3-10
Author(s):  
Ljubica Adji Andov ◽  
Marija Karapandzova ◽  
Blagica Jovanova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj Karanfilova ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dependent Manner ◽  
Flavonoid Content ◽  
Concentration Dependent Manner ◽  
Ic50 Values ◽  
Antioxidant Potency ◽  
Ribose Degradation ◽  
Dpph Radical Scavenging

Evaluation of the antioxidant potential of methanol extract of Chenopodium botrys L. (Amaranthaceae) collected from six different locations in Republic of Macedonia was performed. Several methods were used for testing the antioxidative activity: 1) 2,2-diphenyl1-picrylhydrazyl (DPPH) radical scavenging assay, 2) ferric reduction power assay (FRAP), 3) inhibition of H2 O2 activity, 4) non-sitespecific hydroxyl radical-catalyzed 2-deoxy-D-ribose degradation (NSSOH) and 5) site-specific hydroxyl radical-catalyzed 2-deoxy-D-ribose degradation (SSOH). The IC50 values ranged from 0.26-3.10 mg/mL, 3.01-12.71 mg/mL and 2.60-12.29 mg/mL, for DPPH, NSSOH and SSOH assays, respectively. The H2 O2 inhibition activity and the ferric reducing power capacity were from 28.84-46.56% and 26.14- 43.40%, respectively. The obtained data establish the antioxidant potency in concentration-dependent manner. Additionally, total phenols (TPC) and total flavonoid content (TFC) were determined. The estimated values ranged from 27.77-71.25 mg GAE/g DW and from 7.35- 16.33 mg QE/g DW, respectively

Sign in / Sign up

Export Citation Format

Share Document