scholarly journals Impact of Cross-Linking of Collagen Matrices on Tissue Regeneration in a Rabbit Calvarial Bone Defect

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3740
Author(s):  
Masako Fujioka-Kobayashi ◽  
Elena Andrejova ◽  
Hiroki Katagiri ◽  
Benoit Schaller ◽  
Anton Sculean ◽  
...  
Keyword(s):  
Bone Formation ◽  
Tissue Regeneration ◽  
Fibrous Tissue ◽  
Giant Cells ◽  
Cross Linking ◽  
Tissue Formation ◽  
Calvarial Bone ◽  
Collagen Matrices ◽  
Post Surgery ◽  
The Cross

The cross-linking of collagen matrices (Cl_CM) may provide volume-stable enhanced defect regeneration when compared to non-cross-linked matrices (Ncl_CM). The aim of the present study was to investigate the bone forming potential of collagen matrices (CMs) and the effects of cross-linking CMs in a rabbit calvaria defect model. (1) Empty controls (n = 6), (2) Ncl_CM (n = 8), and (3) Cl_CM (n = 8) were selected to be observed for the healing in 10 mm critical-sized calvarial bone defects. The potential for the bone as well as the connective tissue formation were evaluated by micro-CT and histomorphometry at three months post-surgery. There were no statistically significant differences in terms of new bone volume in the defects between the groups. However, the Cl_CM induced significantly greater fibrous tissue regeneration (5.29 ± 1.57 mm2) when compared to the controls (3.51 ± 0.93 mm2) by histomorphometry. The remnants of collagen fibers with immune cells, including macrophages and giant cells, were occasionally observed in the Cl_CM group but not in the Ncl_CM group. In conclusion, the cross-linking of collagen did not influence the potential for bone formation. Nevertheless, Cl_CM might be advantageous for the maintenance of fibrous tissue volume without disturbing bone formation in the defects.

scholarly journals Role of Adult Tissue-Derived Pluripotent Stem Cells in Bone Regeneration

2019 ◽  
Vol 16 (1) ◽  
pp. 198-211
Author(s):  
Liudmila Leppik ◽  
K. Sielatycka ◽  
D. Henrich ◽  
Z. Han ◽  
H. Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Progenitor Cells ◽  
Bone Healing ◽  
Pluripotent Stem Cells ◽  
Mononuclear Cells ◽  
Fibrous Tissue ◽  
Female Rats ◽  
Post Surgery

Abstract Background Bone marrow-derived mononuclear cells (BM-MNC) consist of a heterogeneous mix of mesenchymal stem cells (MSC), hematopoietic progenitor cells (HPC), endothelial progenitor cells (EPC), monocytes, lymphocytes and pluripotent stem cells. Whereas the importance of MSC and EPC has been well documented in bone healing and regeneration studies, the role of pluripotent stem cells is still poorly understood. In the present study we evaluated if and how Very Small Embryonic Like cells (VSEL), isolated from rat BM-MNC, contribute to bone healing. Methods Large bone defects were made in the femurs of 38 Sprague Dawley female rats and treated with β-TCP scaffold granules seeded with male VSEL; BM-MNC, VSEL-depleted BM-MNC or scaffold alone, and bone healing was evaluated at 8 weeks post-surgery. Results Bone healing was significantly increased in defects treated with VSEL and BM-MNC, compared to defects treated with VSEL-depleted BM-MNC. Donor cells were detected in new bone tissue, in all the defects treated with cells, and in fibrous tissue only in defects treated with VSEL-depleted BM-MNC. The number of CD68+ cells was the highest in the VSEL-depleted group, whereas the number of TRAP positive cells was the lowest in this group. Conclusions Based on the results, we can conclude that VSEL play a role in BM-MNC induced bone formation. In our rat femur defect model, in defects treated with VSEL-depleted BM-MNC, osteoclastogenesis and bone formation were decreased, and foreign body reaction was increased.

scholarly journals A novel fibre-ensemble level constitutive model for exogenous cross-linked collagenous tissues

2016 ◽  
Vol 6 (1) ◽  
pp. 20150090 ◽  
Author(s):  
Michael S. Sacks ◽  
Will Zhang ◽  
Silvia Wognum
Keyword(s):  
Constitutive Model ◽  
Soft Tissues ◽  
Fibrous Tissue ◽  
Collagen Fibre ◽  
Tissue Response ◽  
Loading Path ◽  
Cross Linking ◽  
The Matrix ◽  
The Cross ◽  
Collagenous Tissues

Exogenous cross-linking of soft collagenous tissues is a common method for biomaterial development and medical therapies. To enable improved applications through computational methods, physically realistic constitutive models are required. Yet, despite decades of research, development and clinical use, no such model exists. In this study, we develop the first rigorous full structural model (i.e. explicitly incorporating various features of the collagen fibre architecture) for exogenously cross-linked soft tissues. This was made possible, in-part, with the use of native to cross-linked matched experimental datasets and an extension to the collagenous structural constitutive model so that the uncross-linked collagen fibre responses could be mapped to the cross-linked configuration. This allowed us to separate the effects of cross-linking from kinematic changes induced in the cross-linking process, which in turn allowed the non-fibrous tissue matrix component and the interaction effects to be identified. It was determined that the matrix could be modelled as an isotropic material using a modified Yeoh model. The most novel findings of this study were that: (i) the effective collagen fibre modulus was unaffected by cross-linking and (ii) fibre-ensemble interactions played a large role in stress development, often dominating the total tissue response (depending on the stress component and loading path considered). An important utility of the present model is its ability to separate the effects of exogenous cross-linking on the fibres from changes due to the matrix. Applications of this approach include the utilization in the design of novel chemical treatments to produce specific mechanical responses and the study of fatigue damage in bioprosthetic heart valve biomaterials.

Bovine Anorganic Bone Graft Associated with Platelet-Rich Plasma: Histologic Analysis in Rabbit Calvaria

2011 ◽  
Vol 37 (5) ◽  
pp. 511-518 ◽  
Author(s):  
Flaviana Soares Rocha ◽  
Lara Maria Alencar Ramos ◽  
Jonas Dantas Batista ◽  
Darceny Zanetta-Barbosa ◽  
Eloísa Amália Vieira Ferro ◽  
...  
Keyword(s):  
Bone Formation ◽  
Platelet Rich Plasma ◽  
Fibrous Tissue ◽  
Giant Cells ◽  
Autogenous Bone ◽  
Bone Lesions ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Significant Difference ◽  
Defect Area

Autogenous bone tissue has regeneration potential; however, this capacity may not be sufficient in larger bone defects. The aim of this study is to histologically evaluate anorganic bovine bone grafts (GenOx Inorg) with or without platelet-rich plasma (PRP). Two bone lesions were created in calvaria of 12 rabbits. The 24 surgical lesions were separated into 3 groups: coagulous, anorganic, and anorganic with PRP. At the 4-week time point, the animals were euthanized and the grafted area removed, fixed in formalin 10% with phosphate buffered saline, 0.1 M, and embedded in paraffin. The histologic parameters analyzed were new bone filling the defect area, presence of giant cells and particles of the graft, and new bone formation associated with the particles. In the coagulous group, defects were filled with fibrous tissue that attached the periosteum and little bone neoformation in the periphery. In anorganic groups with or without PRP, little new bone formation in the periphery of the defect was observed; however, in the center of some defects there was new bone. Moderate presence of giant cells and little new bone formation was associated with the innumerous graft particles. Histologic results revealed no statistically significant differences among the defects new bone fill between the studied groups (P  =  .64). There was no significant difference in the number of giant cells (P  =  .60), graft particles (P  =  .46), and new bone formation around graft particles (P  =  .26), whether PRP was added or not. Anorganic bone, isolated or mixed with PRP, was biocompatible and osteoconductive, while maintaining bone volume.

EFFECTS OF CHITOSAN/β-TCP MICROSPHERES ON RABBIT CRANIAL AND CONDYLE DEFECTS HEALING: A PRELIMINARY STUDY

2008 ◽  
Vol 20 (04) ◽  
pp. 239-248 ◽  
Author(s):  
Shyh Ming Kuo ◽  
Li-Chun Lin ◽  
Pei Hua Tsai ◽  
Gregory Cheng-Chie Niu ◽  
Shwu Jen Chang
Keyword(s):  
Bone Formation ◽  
Fine Particles ◽  
Fibrous Tissue ◽  
Control Group ◽  
Tissue Formation ◽  
New Bone Formation ◽  
Group A ◽  
Group B ◽  
Fibrous Tissues ◽  
Electrostatic System

Two kinds of chitosan/β-TCP microspheres were prepared; one was by a traditional emulsion technique (Group A), and the other was by a high-voltage electrostatic system (Group B). Both of the microspheres exhibited good sphericity and the β-TCP fine particles were well trapped inside the chitosan based particles. After 60-day shaking, Group A and Group B degraded by about 40% and 80% of initial weight, respectively. Two models of bone defects were created in rabbits included for a series of randomized blind pilot study. In the cranial model, two equal 10 mm diameter cranial defects were created. In condyle model, two equal 5 mm femur condyle defects were created on each hind leg, and underwent the same grafting treatment. After 4 weeks of implantation, both the cranial and condyle sites filled with Group A were shown to be surrounded by fibrous tissues with the presence of osteoblasts. In Group B, only condyle site showed the presence of osteoblasts. In contrast, fibrous tissue formation was seen on the control group after 4 weeks of healing. After 8 weeks of implantation, the condyle sites filled with Group A and Group B showed the presence of new bone formation as compared to control group. However, there was no obvious new bone formations in the cranial sites filled with both Group A and Group B. The same events were observed in the cranial sites after 12 weeks of implantation. In contrast, new bone formation was seen on the condyle sites in all three groups after 12 weeks of implantation.

scholarly journals Organic Bovine Graft Associated With PRP In Rabbit Calvaria

2011 ◽  
Vol 15 (02) ◽  
pp. 208-213
Author(s):  
Lara Maria Alencar Ramos ◽  
Jonas Dantas Batista ◽  
Darceny Zanetta-Barbosa ◽  
Paula Dechichi ◽  
Flaviana Soares Rocha
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Fibrous Tissue ◽  
Giant Cells ◽  
Histological Evaluation ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Organic Group ◽  
Group I ◽  
Significant Difference

Summary Introduction: Repairing large bone defects is a huge challenge that reconstructive surgery currently faces. Objective: The objective of this study was to perform the histological evaluation of bone repair in rabbit calvaria when using bovine bone graft (Gen-ox-organic®) associated with platelet-rich plasma (PRP). Method: 12 rabbits were used and two bone fragments were bilaterally removed from calvaria. Then, 24 surgical sites were randomly divided into 3 groups: coagulum (group I), organic (group II) and PRP-included organic (group III). After four weeks, the animals were sacrificed and the grafted area removed, fixed in 10% formalin with PBS 0.1 M, and embedded in paraffin. Study method: The analyzed histological parameters were: defective area filled with the newly-formed bone, graft's giant cells and particles, as well as the new bone formation associated with the particles. Group I's defects were filled with fibrous tissue attaching the periosteum and revealed a little bone formation peripherally. In both groups II and III, a similar standard was noticed in addition to the absence of graft particles and giant cells. There was no significant difference in the number of giant cells, graft particles and new bone formation around the particles between the grafted material and the PRP-related group. Conclusion: The results achieved indicate that the organic biomaterial neither separately nor jointly with PRP improves bone regeneration.

scholarly journals Porous Ti6Al4V Scaffold Directly Fabricated by Sintering: Preparation andIn VivoExperiment

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Xuesong Zhang ◽  
Guoquan Zheng ◽  
Jiaqi Wang ◽  
Yonggang Zhang ◽  
Guoqiang Zhang ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Proteins ◽  
Bone Ingrowth ◽  
Fibrous Tissue ◽  
Porous Titanium ◽  
Host Bone ◽  
Tissue Formation ◽  
Long Term Stability ◽  
Increase Bone Formation

The interface between the implant and host bone plays a key role in maintaining primary and long-term stability of the implants. Surface modification of implant can enhance bone ingrowth and increase bone formation to create firm osseointegration between the implant and host bone and reduce the risk of implant losing. This paper mainly focuses on the fabricating of 3-dimensiona interconnected porous titanium by sintering of Ti6Al4V powders, which could be processed to the surface of the implant shaft and was integrated with bone morphogenetic proteins (BMPs). The structure and mechanical property of porous Ti6Al4V was observed and tested. Implant shaft with surface of porous titanium was implanted into the femoral medullary cavity of dog after combining with BMPs. The results showed that the structure and elastic modulus of 3D interconnected porous titanium was similar to cancellous bone; porous titanium combined with BMP was found to have large amount of fibrous tissue with fibroblastic cells; bone formation was significantly greater in 6 weeks postoperatively than in 3 weeks after operation. Porous titanium fabricated by powders sintering and combined with BMPs could induce tissue formation and increase bone formation to create firm osseointegration between the implant and host bone.

Photo Cross-linkable Biopolymers for Cornea Tissue Healing

2021 ◽  
Vol 16 ◽  
Author(s):  
Negar Nozari ◽  
Esmaeil Biazar ◽  
Mahshad Kamalvand ◽  
Saeed Heidari Keshel ◽  
Shervin Shirinbakhsh
Keyword(s):  
Controlled Release ◽  
Physical Properties ◽  
Tissue Regeneration ◽  
Cross Linking ◽  
Tissue Adhesives ◽  
Tissue Healing ◽  
Radiation Sources ◽  
Mechanical And Physical Properties ◽  
The Cross

: Light can act as an effective and strong agent for the cross-linking of biomaterials and tissues and is recognized as a safe substitute for chemical cross-linkers to modify mechanical and physical properties and promote biocompatibility. This review focuses on the research about cross-linked biomaterials with different radiation sources such as Laser or Ultraviolet (UV) that can be applied as scaffolds, controlled release systems, and tissue adhesives for cornea healing and tissue regeneration.

scholarly journals Bone healing in critically sized defects in rat calvaria, transplanted with chitosan alone, or associated with collagen and / or chondroitin sulfate:histological and histomorphometric pilot study

2021 ◽  
Vol 6 (1) ◽  
pp. 9-13
Author(s):  
Cynthia Chemaly ◽  
◽  
Mireille Kallassi ◽  
Rawad Samaran ◽  
Roy Abou Fadel ◽  
...  
Keyword(s):  
Bone Formation ◽  
Chondroitin Sulfate ◽  
Group Formation ◽  
Fibrous Tissue ◽  
Bone Defects ◽  
Sulfate Group ◽  
Control Group ◽  
Calvarial Bone ◽  
Group 2 ◽  
Group 1

Background: Chitosan is a natural biopolymer that has gained a special interest in bone regeneration in recent years. Objective: The objective of this study is to show the bone formation obtained following a transplantation of sponges of chitosan alone, chitosan combined with chondroitin sulfate or chitosan combined with chondroitin sulfate and collagen, in rat critical calvarial bone defects. Material and Methods: 12 Wistar rats were divided into 4 groups of 3 rats each. Critically sized bone defects were made in calvaria, and grafted by sponges of:collagen / chitosan / chondroitin (group 1), chitosan (group 2), chitosan / chondroitin sulfate (group3). Bone defects of group 4 remained empty for control. The animals were sacrificed 12 weeks after the surgery. Results: Histological analysis showed the formation of lamellar bone in the chitosan group. In the chitosan / chondroitin sulfate group, formation of a less mature bone than that of the chitosan group was also observed. However, the least bone formation was observed in the collagen / chitosan / chondroitin sulfate group. Histological sections showed the presence of fibrous tissue. The results for this group are similar to the control group. In groups 2 and 3, the materials appear completely resorbed while in group 1 the resorption of the matrix was incomplete. Conclusion: Despite the size of the sample, this study has shown that chitosan alone or in combination with chondroitin sulfate promotes bone formation. On the other hand, the combination chitosan / chondroitin sulfate / collagen showed a negative result.

Sign in / Sign up

Export Citation Format

Share Document