scholarly journals Formation and Investigation of Physicochemical, Biological and Bacteriostatic Properties of Nanocomposite Foils Containing Silver Nanoparticles and Graphene Oxide in Hyaluronic Acid Matrix

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3377
Author(s):  
Karen Khachatryan ◽  
Lusine Khachatryan ◽  
Marcel Krzan ◽  
Magdalena Krystyjan ◽  
Lidia Krzemińska-Fiedorowicz ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Hyaluronic Acid ◽  
Structural Changes ◽  
Fourier Transforms ◽  
Sodium Hyaluronate ◽  
Size Exclusion ◽  
Biomedical Sciences ◽  
Vis Spectroscopy ◽  
Wide Range

Natural polysaccharides, including hyaluronic acid, find a wide range of applications in biomedical sciences. There is a growing interest in nanocomposites containing hyaluronic acid and nanoparticles such as nanometals or graphene. In this study, we prepared foils of pure sodium hyaluronate and sodium hyaluronate containing nanosilver, graphene oxide, nanosilver/graphene oxide and characterized their properties. UV-vis spectroscopy and scanning electron microscopy (SEM) confirmed the formation of 10–20 nm silver nanoparticles. The structural changes were investigated using Fourier transforms infrared (FTIR) spectra and size exclusion chromatography. The obtained results suggest changes in molecular weights in the samples containing nanoparticles, which was highest in a sample containing nanosilver/graphene oxide. We also assessed the mechanical properties of the foils (thickness, tensile strength and elongation at break) and their wettability. The foils containing nanosilver and nanosilver/graphene oxide presented bacteriostatic activity against E. coli, Staphylococcus spp. and Bacillus spp., which was not observed in the control and sample containing graphene oxide. The composites containing graphene oxide and nanosilver/graphene oxide exhibited a cytotoxic effect on human melanoma WM266-4 cell lines (ATCC, Manassas, VA, USA).

scholarly journals Synthesis, characterization and antibacterial activity of silver nanoparticles using Rhazya stricta

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e6086 ◽  
Author(s):  
Adeeb Shehzad ◽  
Munibah Qureshi ◽  
Saima Jabeen ◽  
Rizwan Ahmad ◽  
Amira H. Alabdalall ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Metallic Nanoparticles ◽  
Fourier Transforms ◽  
Root Extract ◽  
Gram Positive ◽  
Rhazya Stricta ◽  
Vis Spectroscopy ◽  
Wide Range ◽  
Pharmaceutical Industries

Background Green synthesis of metallic nanoparticles has gained significant attention in the field of nanomedicine as an environment-friendly and cost-effective alternative in comparison with other physical and chemical methods. Several metals such as silver, gold, iron, titanium, zinc, magnesium and copper have been subjected to nanoformulation for a wide range of useful applications. Silver nanoparticles (AgNPs) are playing a major role in the field of nanomedicine and nanotechnology. They are widely used in diagnostics, therapeutic and pharmaceutical industries. Studies have shown potential inhibitory antimicrobial, anti-inflammatory and antiangiogenesis activities of AgNPs. Methods AgNPs have been synthesized using silver nitrate and methanolic root extract of Rhazya stricta that belongs to the Apocynaceae family. Stability and dispersion of nanoparticles were improved by adding xylitol. Synthesized nanoparticles were characterized by UV–Vis spectroscopy, scanning electron microscopy, energy dispersive spectroscopy, X-ray diffractometer and Fourier transforms infrared spectroscopy. Furthermore, the antibacterial effect of the plant extract and the nanoparticles were evaluated against gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria. Results The average size of AgNPs synthesized, was 20 nm with the spherical shape. Rhazya stricta based nanoparticles exhibited improved antibacterial activity against both gram-positive and negative strains.

Final Report of the Safety Assessment of Hyaluronic Acid, Potassium Hyaluronate, and Sodium Hyaluronate

2009 ◽  
Vol 28 (4_suppl) ◽  
pp. 5-67 ◽  
Author(s):  
Lillian C. Becker ◽  
Wilma F. Bergfeld ◽  
Donald V. Belsito ◽  
Curtis D. Klaassen ◽  
James G. Marks ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Safety Assessment ◽  
Animal Studies ◽  
Cancer Metastasis ◽  
Sodium Hyaluronate ◽  
Final Report ◽  
Bacterial Fermentation ◽  
Wide Range ◽  
Exposure Routes ◽  
Sodium And Potassium

Hyaluronic acid, sodium hyaluronate, and potassium hyaluronate function in cosmetics as skin conditioning agents at concentrations up to 2%. Hyaluronic acid, primarily obtained from bacterial fermentation and rooster combs, does penetrate to the dermis. Hyaluronic acid was not toxic in a wide range of acute animal toxicity studies, over several species and with different exposure routes. Hyaluronic acid was not immunogenic, nor was it a sensitizer in animal studies. Hyaluronic acid was not a reproductive or developmental toxicant. Hyaluronic acid was not genotoxic. Hyaluronic acid likely does not play a causal role in cancer metastasis; rather, increased expression of hyaluronic acid genes may be a consequence of metastatic growth. Widespread clinical use of hyaluronic acid, primarily by injection, has been free of significant adverse reactions. Hyaluronic acid and its sodium and potassium salts are considered safe for use in cosmetics as described in the safety assessment.

scholarly journals Synthesis of Silver and Gold Nanoparticles in Sodium Alginate Matrix Enriched with Graphene Oxide and Investigation of Properties of the Obtained Thin Films

2021 ◽  
Vol 11 (9) ◽  
pp. 3857
Author(s):  
Nikola Nowak ◽  
Wiktoria Grzebieniarz ◽  
Gohar Khachatryan ◽  
Karen Khachatryan ◽  
Anna Konieczna-Molenda ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Sodium Alginate ◽  
Size Exclusion ◽  
Scanning Calorimetry ◽  
Molecular Weights ◽  
Vis Spectroscopy ◽  
The Matrix ◽  
Exclusion Chromatography ◽  
20 Nm ◽  
Multiangle Laser Light Scattering

Polymer nanocomposites containing nanometals became a subject of interest due to their bactericidal properties. Different polysaccharides have been used as matrices for nanosilver and nanogold synthesis. In this study, we present a novel, environmentally friendly method for the preparation of sodium alginate/nanosilver/graphene oxide (GOX) and sodium alginate/nanogold/graphene oxide GOX nanocomposites and their characteristics. The formation of approximately 10–20 nm ball-shaped Ag and Au nanoparticles was confirmed by UV–vis spectroscopy, scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectra. The incorporation of GOX sheets within the ALG matrix improved the thermal stability of the nanocomposites film, which was measured using the differential scanning calorimetry (DSC). We also estimated the molecular weights of polysaccharide chains of the matrix with the size exclusion chromatography coupled with multiangle laser light scattering and refractometric detectors (HPSEC-MALLS-RI). The composites were more prone to enzymatic hydrolysis. The strongest bacteriostatic activity was observed for the sample containing nanosilver.

scholarly journals Comparative Analysis of Nanosilver Particles Synthesized by Different Approaches and Their Antimicrobial Efficacy

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Hala M. Abdelmigid ◽  
Maissa M. Morsi ◽  
Nahed Ahmed Hussien ◽  
Amal Ahmed Alyamani ◽  
Noha Moslah Al Sufyani
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Fourier Transforms ◽  
Antimicrobial Effect ◽  
Trisodium Citrate ◽  
Biological Synthesis ◽  
Bacterial Strains ◽  
Fruit Peel ◽  
Vis Spectroscopy ◽  
Coffee Ground

Silver nanoparticles (AgNPs) were extensively used in different fields worldwide. There is a continued increase in their productions to fulfill various uses. Biological and chemical AgNP syntheses were the most popular mechanisms in this field. Agrowastes are rich in proteins, phenolics, and flavonoids that could act as bioreductant agents in AgNP biological synthesis. The present study was aimed at synthesizing AgNPs via chemical and biological methods using trisodium citrate, pomegranate fruit peel, and coffee ground waste extracts. Moreover, silver nanoparticles were monitored by UV-vis spectroscopy and characterized using zeta potential, size distribution mean, scanning electron microscope (SEM), X-ray diffractometer (XRD), and Fourier transforms infrared spectroscopy (FTIR). Four pathogenic bacterial strains (Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa, and MRSA) were used to assess the antimicrobial effect of the synthesized AgNPs (2, 4, and 8 mg/ml). Results report the successful formation of silver nanoparticles chemically (AgNPs_Chem) and biologically by using pomegranate peel extract (AgNPs_PPE) and coffee ground waste extract (AgNPs_CE) due to the change of color to dark brown that is confirmed by UV-vis sharp absorption spectra at specific wavelengths. Characterization using SEM and XRD revealed their crystalline shape with a mean size of AgNPs _ Chem = 62.75 , AgNPs _ CE = 273.7   nm , and AgNPs _ PPE = 591.9   nm . AgNPs_Chem show higher negativity of zeta potential (−46.7 mV) than AgNPs_CE (−12.6 mV), followed by AgNPs_PPE (−7.98 mV), which had the least stability. All the synthesized AgNPs show antimicrobial potential on all selected strains. However, 8 mg/ml shows the most effective concentration and has more efficiency on K. pneumoniae than others. Overall, the results highlight that the use of agrowastes could be an ecofriendly way to synthesize AgNPs biologically that have the same antimicrobial effect as the chemically synthesized AgNPs.

scholarly journals Development of Highly pH-Sensitive Hybrid Membranes by Simultaneous Electrospinning of Amphiphilic Nanofibers Reinforced with Graphene Oxide

2019 ◽  
Vol 10 (2) ◽  
pp. 23 ◽  
Author(s):  
Mohsen Gorji ◽  
Ali Sadeghianmaryan ◽  
Hossein Rajabinejad ◽  
Saman Nasherolahkam ◽  
Xiongbiao Chen
Keyword(s):  
Graphene Oxide ◽  
Response Time ◽  
Color Change ◽  
Fiber Diameter ◽  
Chemical Vapor ◽  
Ph Sensitive ◽  
Solution Ph ◽  
Vis Spectroscopy ◽  
Wide Range ◽  
Nanofibrous Membranes

Nanofibrous-based pH sensors have shown promise in a wide range of industrial and medical applications due to their fast response time and good mechanical properties. In the present study, we fabricated pH-sensitive sensors of nanofibrous membranes by electrospinning polyurethane (PU)/poly 2-acrylamido-2-methylpropanesulfonic acid (PAMPS)/graphene oxide (GO) with indicator dyes. The morphology of the electrospun nanofibers was examined using scanning electron microscopy (SEM). The effect of hydrophilic polymer ratio and concentration of GO on the sensing response time was investigated. The sensitivity of the membranes was studied over a wide pH range (1–8) in solution tests, with color change measured by calculating total color difference using UV-vis spectroscopy. The membranes were also subjected to vapor tests at three different pH values (1, 4, 8). SEM results show the successful fabrication of bimodal fiber diameter distributions of PU (mean fiber diameter 519 nm) and PAMPS (mean fiber diameter 78 nm). Sensing response time decreased dramatically with increasing concentrations of PAMPS and GO. The hybrid hydrophobic/hydrophilic/GO nanofibrous membranes are capable of instantly responding to changes in solution pH as well as detecting pH changes in chemical vapor solution in as little as 7 s.

scholarly journals Biosynthesis of silver nanoparticles using Pomegranate juice extract and its antibacterial activity

2016 ◽  
Vol 4 (3) ◽  
pp. 254-258 ◽  
Author(s):  
Mona Hussein Ibraheim ◽  
A.A. Ibrahiem ◽  
T. R. Dalloul
Keyword(s):  
Silver Nanoparticles ◽  
Fourier Transforms ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Pomegranate Juice ◽  
Face Centered Cubic ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Biosynthesized Agnps ◽  
High Level

Green synthesis of silver nanoparticles (AgNPs) from silver nitrate was carried out using aqueous Pomegranate juice extract (PJE) as a reducing agent. The formation of AgNPs was characterized by UV-visible (UV–vis) spectroscopy, transmission electron microscopy (TEM), Fourier transforms infrared spectroscopy and X-ray diffraction (XRD). Surface Plasmon resonance (SPR) of ∼420-423 nm confirmed the earlier formation of AgNPs. TEM and XRD analysis showed that the AgNPs with an average diameter of 23 nm are crystalline in nature and have face-centered cubic geometry. The antibacterial efficiency of AgNPs against Escherchia coli and Staphylococcus aureus showed high level of inhibition. Further, the zone of inhibition increased with the increase in the concentration of silver nanoparticles. These studies are quite useful as it shows the utility of green nanotechnology for the synthesis of silver nanoparticles without any toxic residuals and byproducts. The efficient antimicrobial activity of biosynthesized AgNPs proves the application potential in the area of nano-medicine.Int J Appl Sci Biotechnol, Vol 4(3): 254-258

scholarly journals Evaluating the Antibacterial Activity of AgGO Nanocomposite Against Clinical Isolate Bacteria

2019 ◽  
Vol 54 (6) ◽  
Author(s):  
Marwah Al-Ogaidi ◽  
Israa Al-Ogaidi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Laser Ablation ◽  
Silver Nanoparticle ◽  
Pathogenic Bacteria ◽  
Fourier Transforms ◽  
Gram Positive Bacteria ◽  
Laser Ablation Method ◽  
Fast Synthesis

This study discusses the synthesis, characterization, and activity of silver nanoparticles, along with silver graphene oxide, against pathogenic bacteria. Silver nanoparticles are prepared by laser ablation and fabricated with silver graphene oxide. The synthesized silver nanoparticles and silver graphene oxide were characterized through UV-Vis spectrophotometer, Fourier-transforms spectroscopy, and zeta potential. The antimicrobial performance of silver nanoparticles and silver graphene oxide nanoparticles were monitored by the contradiction of gram-negative bacteria (Escherichia.coli and Acenetobacter bumannii), and gram positive bacteria: Staphylococcus aureus. Different concentrations of silver and silver graphene oxide have been investigated to monitor their antibacterial activity against bacterial isolates. The results indicate that the nanocomposites have antibacterial activity and the antibacterial effect increases with increased concentration of nanocomposites. Laser ablation method, a fast synthesis technique, has been used in the current study to prepare silver nanoparticle and silver nanoparticle-graphene oxide nanocomposites.

scholarly journals Martini Coarse-Grained Model of Hyaluronic Acid for the Structural Change of Its Gel in the Presence of Monovalent and Divalent Salts

2020 ◽  
Vol 21 (13) ◽  
pp. 4602 ◽  
Author(s):  
Raj Kumar ◽  
Young Kyu Lee ◽  
Yong Seok Jho
Keyword(s):  
Hyaluronic Acid ◽  
Large Scale ◽  
Structural Changes ◽  
Coarse Grained ◽  
Radius Of Gyration ◽  
Main Role ◽  
Structural Formation ◽  
Wide Range ◽  
Distinct Property ◽  
End Distance

Hyaluronic acid (HA) has a wide range of biomedical applications including the formation of hydrogels, microspheres, sponges, and films. The modeling of HA to understand its behavior and interaction with other biomolecules at the atomic level is of considerable interest. The atomistic representation of long HA polymers for the study of the macroscopic structural formation and its interactions with other polyelectrolytes is computationally demanding. To overcome this limitation, we developed a coarse grained (CG) model for HA adapting the Martini scheme. A very good agreement was observed between the CG model and all-atom simulations for both local (bonded interactions) and global properties (end-to-end distance, a radius of gyration, RMSD). Our CG model successfully demonstrated the formation of HA gel and its structural changes at high salt concentrations. We found that the main role of CaCl2 is screening the electrostatic repulsion between chains. HA gel did not collapse even at high CaCl2 concentrations, and the osmotic pressure decreased, which agrees well with the experimental results. This is a distinct property of HA from other proteins or polynucleic acids which ensures the validity of our CG model. Our HA CG model is compatible with other CG biomolecular models developed under the Martini scheme, which allows for large-scale simulations of various HA-based complex systems.

Effect of pH on the Environmentally Friendly Fabrication of Silver Nanoparticles Using Rambutan Peel Extract

2019 ◽  
Vol 824 ◽  
pp. 149-155 ◽  
Author(s):  
Siriporn Phongtongpasuk ◽  
Titika Norasingsatorn ◽  
Niti Yongvanich
Keyword(s):  
Silver Nanoparticles ◽  
Size Distribution ◽  
Basic Solution ◽  
Human Pathogens ◽  
Solution Ph ◽  
Effect Of Ph ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Wide Range ◽  
Peel Extract

Silver nanoparticles (AgNPs) have been intensively researched because of their wide range of applications in the areas of catalysis, optics, antimicrobials and biomaterials production. The pH of the reaction mixture is one of the crucial parameters for improving the properties of AgNP, including size, morphology and agglomeration. In this study, AgNPs were synthesized by a biological method using rambutan peel extract. The effect of pH on the resultant biogenic AgNPs was observed by varying the pH values to be 2, 4, 6, 8, 10 and 12. The biosynthesized AgNPs was characterized by UV-Vis spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), Energy-dispersive X-ray spectroscopy (EDX) and Zeta potential analysis. The results showed the formation of AgNPs with size ranging from 15-120 nm in diameter. Phytochemical compounds in the extract are likely responsible for the reduction and stabilization of AgNPs. The shape, size and stability of AgNPs depend on the pH of the solution. AgNPs prepared at pH 2, 4 and 6 were of various shapes with a large size distribution. Those synthesized in basic solution (pH 8, 10 and 12) were spherical in shape. The results indicate that pH 8 is optimal for synthesis of AgNP as it offers pureness and fineness with narrow size distribution. Furthermore, biosynthesized AgNP exhibits antibacterial activity against the growth of selected human pathogens.

scholarly journals Controlling of oral pathogens using ginger oleoresin mediated silver nanoparticles

2019 ◽  
Vol 10 (4) ◽  
pp. 2988-2991 ◽  
Author(s):  
Karthikeyan Gayathri ◽  
Anitha Roy ◽  
Thangavelu Lakshmi ◽  
Rajeshkumar S
Keyword(s):  
Silver Nanoparticles ◽  
Low Cost ◽  
Diffusion Method ◽  
Natural Substances ◽  
Antimicrobial Sensitivity ◽  
Vis Spectroscopy ◽  
Wide Range ◽  
Test Organisms ◽  
Oral Pathogens ◽  
Agar Well Diffusion Method

Silver nanoparticles have become a substance of great interest among researches in the present time due to its low cost and its wide range of applications in various fields. Moreover, recently, green nanotechnology has been gaining popularity because of its exclusion of damaging reagents and cost-effectiveness. In this study, ginger oleoresin mediated silver nanoparticles were prepared and confirmed with UV-Vis spectroscopy. The ginger oleoresin mediated silver nanoparticles were then tested for its antibacterial activity using agar well diffusion method against Staphylococcus aureus, Streptococcus mutans, and Enterococcus faecalis. The antimicrobial sensitivity was found to be highest against Streotococcus mutans among the test organisms. Silver nanoparticle synthesised using natural substances can be used in control of oral pathogens.

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