scholarly journals Effect of Precursor Deficiency Induced Ca/P Ratio on Antibacterial and Osteoblast Adhesion Properties of Ag-Incorporated Hydroxyapatite: Reducing Ag Toxicity

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3158
Author(s):  
Ozkan Gokcekaya ◽  
Celaletdin Ergun ◽  
Thomas J. Webster ◽  
Abdurrahman Bahadir ◽  
Kyosuke Ueda ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Processing Parameters ◽  
Biological Properties ◽  
Precipitation Method ◽  
Adhesion Properties ◽  
Carbonate Substitution ◽  
Osteoblast Adhesion

Ag-containing hydroxyapatite (HA) can reduce risks associated with bacterial infections which may eventually require additional surgical operations to retrieve a failed implant. The biological properties of HA in such applications are strongly affected by its composition in terms of dopants as well as Ca/P stoichiometry, which can be easily controlled by altering processing parameters, such as precursor concentrations. The objective of this in vitro study was to understand the effect of variations in HA precursor solutions on antibacterial properties against Escherichia coli (E. coli) and for promoting osteoblast (bone-forming cell) adhesion on Ag incorporated HA (AgHA) which has not yet been investigated. For this, two groups of AgHAs were synthesized via a precipitation method by adjusting precursor reactants with a stoichiometric value of 1.67, being either (Ca + Ag)/P (Ca-deficient) or Ca/(P + Ag) (P-deficient), and were characterized by XRD, FTIR, and SEM-EDS. Results showed that Ag+ incorporated into the Ca2+ sites was associated with a corresponding OH− vacancy. Additional incorporation of CO32− into PO43− sites occurred specifically for the P-deficient AgHAs. While antibacterial properties increased, osteoblast adhesion decreased with increasing Ag content for the Ca-deficient AgHAs, as anticipated. In contrast, significant antibacterial properties with good osteoblast behavior were observed on the P-deficient AgHAs even with a lower Ag content, owing to carbonated HA. Thus, this showed that by synthesizing AgHA using P-deficient precursors with carbonate substitution, one can keep the antibacterial properties of Ag in HA while reducing its toxic effect on osteoblasts.

NFLUENCE OF THE DEGREE OF CALCIUM SUBSTITUTION BY ARGENTUM IN TRICALCIUM PHOSPHATE ON ITS BIOLOGICAL PROPERTIES IN VITRO

2013 ◽  
Vol 20 (4) ◽  
pp. 23-28
Author(s):  
V. E Khon ◽  
N. V Zagorodniy ◽  
V. S Komlev ◽  
I. V Fadeev ◽  
V. G Bulgakov ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tricalcium Phosphate ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Biological Properties ◽  
Bacteriostatic Effect ◽  
Dependent Effect ◽  
Dose Dependent ◽  
Dose Dependent Effect

Results of in vitro study of argentum containing tricalcium phosphate (TCPh-Ag) are presented. It is shown that biomaterial does not possess radical forming activity. Argentum containing forms of TCPh render bacteriostatic effect upon Staphylococcus haemolyticus and Escherichia coli. Dose-dependent effect of TCPh-Ag in relation to antibacterial and cytotoxic properties is demonstrated. It is determined that TCPh with rated substitution 0.5 is characterized by moderate cytotoxicity with preservation of antibacterial properties.

scholarly journals Porous Curdlan-Based Hydrogels Modified with Copper Ions as Potential Dressings for Prevention and Management of Bacterial Wound Infection—An In Vitro Assessment

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1893
Author(s):  
Aleksandra Nurzynska ◽  
Katarzyna Klimek ◽  
Iga Swierzycka ◽  
Krzysztof Palka ◽  
Grazyna Ginalska
Keyword(s):  
Bacterial Infections ◽  
Chronic Wounds ◽  
Copper Ions ◽  
Bacterial Colonization ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Wound Dressings ◽  
Wound Fluid ◽  
Potential Safety

Bacterial infections at the wound site still remain a huge problem for current medicine, as they may lead to development of chronic wounds. In order to prevent such infections, there is a need to use wound dressings that possess ability to inhibit bacterial colonization. In this study, three new curdlan-based biomaterials modified with copper ions were fabricated via simple and inexpensive procedure, and their structural, physicochemical, and biological properties in vitro were evaluated. Received biomaterials possessed porous structure, had ability to absorb high amount of simulated wound fluid, and importantly, they exhibited satisfactory antibacterial properties. Nevertheless, taking into account all evaluated properties of new curdlan-based biomaterials, it seems that Cur_Cu_8% is the most promising biomaterial for management of wounds accompanied with bacterial infections. This biomaterial exhibited the best ability to reduce Escherichia coli and Staphylococcus aureus growth and moreover, it absorbed the highest amount of simulated wound fluid as well as enabled optimal water vapor transmission. Furthermore, Cur_Cu_8% biomaterial possessed the best values of selective indexes, which determine its potential safety in vitro. Thus, Cur_Cu_8% hydrogel may be considered as a promising candidate for management of infected wounds as well as it may constitute a good platform for further modifications.

scholarly journals Antibacterial Properties of Canine Platelet-Rich Plasma and Other Non-Transfusional Hemo-Components: An in vitro Study

2021 ◽  
Vol 8 ◽  
Author(s):  
Anna-Rita Attili ◽  
Cristina Iacoucci ◽  
Evelina Serri ◽  
Vincenzo Cuteri ◽  
Andrea Cantalamessa ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Antibacterial Effect ◽  
Platelet Rich Plasma ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Veterinary Antibiotics ◽  
Bacterial Strains

This in vitro study was carried out to evaluate the potential antibacterial properties of canine non-transfusional hemo-components. Therapeutic formulations commonly used for regenerative medicine purposes (platelet-rich plasma, platelet gel, platelet lysate, fibrin glue), considering both leukocyte-rich and leukocyte-poor formulations, but also platelet-poor plasma and activating substances (thrombin, calcium gluconate), were tested to detect elements with potential antimicrobial properties. The antibacterial effect was tested on different bacterial strains (Staphylococcus aureus subspecies aureus, Staphylococcus cohnii subspecies cohnii, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae subspecies pneumoniae) isolated from canine wounds and classified as susceptible, multidrug-, extensively, and pandrug-resistant bacteria toward a known panel of human and veterinary antibiotics. The evaluation was carried out by agar gel diffusion method (Kirby–Bauer) and micro-inhibition in broth using microplates and spectrophotometer reading. The study findings confirmed the hypothesized antibacterial properties of canine non-transfusional hemo-components. A more effective bacteriostatic effect was found against Gram-negative bacteria, drug-resistant too. The presence of leukocytes or platelets does not appear to be essential for the antibacterial effect. Further studies should be conducted to evaluate the exact mechanism of action of the antimicrobial activity. However, non-transfusional hemo-components could be a useful natural aid in controlling bacterial infections in dogs.

scholarly journals 5-O-Demethylnobiletin Alleviates CCl4-Induced Acute Liver Injury by Equilibrating ROS-Mediated Apoptosis and Autophagy Induction

2021 ◽  
Vol 22 (3) ◽  
pp. 1083
Author(s):  
Sukkum Ngullie Chang ◽  
Se Ho Kim ◽  
Debasish Kumar Dey ◽  
Seon Min Park ◽  
Omaima Nasif ◽  
...  
Keyword(s):  
Liver Injury ◽  
Acute Liver Injury ◽  
Elevated Serum ◽  
In Vitro Study ◽  
Biological Properties ◽  
Autophagic Flux ◽  
Serum Aminotransferase ◽  
Ccl4 Treatment

Polymethoxyflavanoids (PMFs) have exhibited a vast array of therapeutic biological properties. 5-O-Demethylnobiletin (5-DN) is one such PMF having anti-inflammatory activity, yet its role in hepatoprotection has not been studied before. Results from in vitro study revealed that 5-DN did not exert a high level of cytotoxicity on HepG2 cells at 40 μM, and it was able to rescue HepG2 cell death induced by carbon tetrachloride (CCl4). Subsequently, we investigated acute liver injury on BALB/c mice induced by CCl4 through the intraperitoneal injection of 1 mL/kg CCl4 and co-administration of 5-DN at (1 and 2 mg/kg) by oral gavage for 15 days. The results illustrated that treatment with 5-DN attenuated CCl4-induced elevated serum aminotransferase (AST)/alanine aminotransferase (ALT) ratio and significantly ameliorated severe hepatic damage such as inflammation and fibrosis evidenced through lesser aberrations in the liver histology of 5-DN dose groups. Additionally, 5-DN efficiently counteracted and equilibrated the production of ROS accelerated by CCl4 and dramatically downregulated the expression of CYP2E1 vitally involved in converting CCl4 to toxic free radicals and also enhanced the antioxidant enzymes. 5-DN treatment also inhibited cell proliferation and inflammatory pathway abnormally regulated by CCl4 treatment. Furthermore, the apoptotic response induced by CCl4 treatment was remarkably reduced by enhanced Bcl-2 expression and noticeable reduction in Bax, Bid, cleaved caspase 3, caspase 9, and apaf-1 expression. 5-DN treatment also induced the conversion of LC3 and promoted the autophagic flux. Conclusively, 5-DN exhibited hepatoprotective effects in vitro and in vivo and prevented liver fibrosis induced by CCl4.

scholarly journals Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite

2021 ◽  
Vol 22 (8) ◽  
pp. 4246
Author(s):  
Muhammad Maqbool ◽  
Qaisar Nawaz ◽  
Muhammad Atiq Ur Atiq Ur Rehman ◽  
Mark Cresswell ◽  
Phil Jackson ◽  
...  
Keyword(s):  
Negative Impact ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Ion Concentration ◽  
Charge Composition ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Molar Ratios ◽  
Human Osteosarcoma Cells

In this study, as a measure to enhance the antimicrobial activity of biomaterials, the selenium ions have been substituted into hydroxyapatite (HA) at different concentration levels. To balance the potential cytotoxic effects of selenite ions (SeO32−) in HA, strontium (Sr2+) was co-substituted at the same concentration. Selenium and strontium-substituted hydroxyapatites (Se-Sr-HA) at equal molar ratios of x Se/(Se + P) and x Sr/(Sr + Ca) at (x = 0, 0.01, 0.03, 0.05, 0.1, and 0.2) were synthesized via the wet precipitation route and sintered at 900 °C. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and cell viability were studied. X-ray diffraction verified the phase purity and confirmed the substitution of selenium and strontium ions. Acellular in vitro bioactivity tests revealed that Se-Sr-HA was highly bioactive compared to pure HA. Se-Sr-HA samples showed excellent antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus carnosus) bacterial strains. In vitro cell–material interaction, using human osteosarcoma cells MG-63 studied by WST-8 assay, showed that Se-HA has a cytotoxic effect; however, the co-substitution of strontium in Se-HA offsets the negative impact of selenium and enhanced the biological properties of HA. Hence, the prepared samples are a suitable choice for antibacterial coatings and bone filler applications.

scholarly journals Long-term antibacterial properties of a nanostructured titanium alloy surface: An in vitro study

2022 ◽  
Vol 13 ◽  
pp. 100176
Author(s):  
Richard Bright ◽  
Daniel Fernandes ◽  
Jonathan Wood ◽  
Dennis Palms ◽  
Anouck Burzava ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Vitro Study ◽  
Alloy Surface ◽  
Nanostructured Titanium

Helical Rosette Nanotubes as a Potentially More Effective Orthopaedic Implant Material

2004 ◽  
Vol 845 ◽  
Author(s):  
Ai Lin Chun ◽  
Hicham Fenniri ◽  
Thomas J. Webster
Keyword(s):  
Tissue Engineering ◽  
Surface Roughness ◽  
Bone Cells ◽  
In Vitro Study ◽  
Orthopaedic Implant ◽  
Rosette Nanotubes ◽  
Osteoblast Adhesion ◽  
Organic Nanotubes ◽  
First Time

ABSTRACTOrganic nanotubes called helical rosette nanotubes (HRN) have been synthesized in this study for bone tissue engineering applications. They possess intriguing properties for various bionanotechnology applications since they can be designed to mimic the nanostructured constituent components in bone such as collagen fibers and hydroxyapatite (Ca5(PO4)3(OH)) which bone cells are naturally accustomed to interacting with. This is in contrast to currently used orthopaedic materials such as titanium which do not possess desirable nanometer surface roughness. The objective of this in vitro study was to determine bone-forming cell (osteoblasts) interactions on titanium coated with HRNs. Results of this study showed for the first time increased osteoblast adhesion on titanium coated with HRNs compared to those not coated with HRNs. In this manner, this study provided evidence that HRNs should be further considered for orthopaedic applications.

scholarly journals A COMPARATIVE EVALUATION OF MICROLEAKAGE, COMPRESSIVE STRENGTH, FLEXURAL STRENGTH AND FLUORIDE RELEASE OF ZIRCONOMER AND KETAC SILVER: AN IN VITRO STUDY.

2020 ◽  
Vol 9 (4) ◽  
Author(s):  
CHARANTEJA VEMAGIRI ◽  
Uloopi KS ◽  
Vinay Chandrappa ◽  
Anusha Ch
Keyword(s):  
Compressive Strength ◽  
Flexural Strength ◽  
Testing Machine ◽  
In Vitro Study ◽  
Biological Properties ◽  
T Test ◽  
Fluoride Release ◽  
Restorative Material ◽  
Glass Ionomer

Background: A successful restorative material forms a better adhesion, resist the microleakage and releases fluoride. However, existing glass ionomer cements cannot be used as a posterior restorative material in stress bearing areas. A new ionomer called Zirconomer, zirconia reinforced glass ionomer claims to exhibit high physical and biological properties. Aim: To assess and compare the microleakage, compressive strength, flexural strength and fluoride release from zirconomer with ketac silver. Materials & Methods: On twenty caries free premolar teeth (10 per each group), a class v cavity was restored with zirconomer and ketac silver. The microleakage was assessed using dye penetration test and stereomicroscope. The compressive and flexural strengths of these materials were measured using Instron Universal testing machine. The amount of fluoride released from the modified ionomers at pH 5 and pH 7 was estimated using Orion fluoride specific electrode. The obtained data was then subjected to statistical analysis. Results: Data was analysed using paired t-test for intergroup comparisons and unpaired t-test for intragroup comparisons. The overall microleakage (0.5±0.48) of zirconomer was significantly less (p=0.000) compared to ketac silver (1.9±0.83). Zirconomer demonstrated a significant higher compressive strength (330.25±60.14), flexural strength (33.058±2.36) than ketac silver (p= 0.000). Zirconomer demonstrated high fluoride release from day 1 to day 7 at both pH 5 and pH 7. Conclusion: Zirconomer demonstrated better physical and biological properties compared with ketac silver.

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