scholarly journals Solution Blow Spinning of Polycaprolactone—Rheological Determination of Spinnability and the Effect of Processing Conditions on Fiber Diameter and Alignment

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1463
Author(s):  
Katarzyna Czarnecka ◽  
Michał Wojasiński ◽  
Tomasz Ciach ◽  
Pawel Sajkiewicz
Keyword(s):  
Tissue Engineering ◽  
Image Analysis ◽  
Polymer Solution ◽  
Fiber Diameter ◽  
Fibrous Tissue ◽  
Qualitative Assessment ◽  
Processing Conditions ◽  
Fiber Morphology ◽  
Solution Blow Spinning ◽  
Polymer Solvent

The growing popularity of solution blow spinning as a method for the production of fibrous tissue engineering scaffolds and the vast range of polymer–solvent systems available for the method raises the need to study the effect of processing conditions on fiber morphology and develop a method for its qualitative assessment. Rheological approaches to determine polymer solution spinnability and image analysis approaches to describe fiber diameter and alignment have been previously proposed, although in a separate manner and mostly for the widely known, well-researched electrospinning method. In this study, a series of methods is presented to determine the processing conditions for the development of submicron fibrous scaffolds. Rheological methods are completed with extensive image analysis to determine the spinnability window for a polymer–solvent system and qualitatively establish the influence of polymer solution concentration and collector rotational speed on fiber morphology, diameter, and alignment. Process parameter selection for a tissue engineering scaffold target application is discussed, considering the varying structural properties of the native extracellular matrix of the tissue of interest.

Biodegradable Micro- and Nanofibers Fabricated Through Electrospinning for Tissue Engineering and Controlled Release Applications

2009 ◽  
Author(s):  
Jia-Chen Kang ◽  
Min Wang ◽  
Xiao-Yan Yuan
Keyword(s):  
Tissue Engineering ◽  
Controlled Release ◽  
Polymer Solution ◽  
Fiber Diameter ◽  
Therapeutic Agents ◽  
The Other ◽  
Average Fiber Diameter ◽  
Needle Size ◽  
Other Hand ◽  
Fibrous Membranes

Electrospinning is a simple and versatile technique for producing micro- and nanofibers. It has been shown that electrospun tissue engineering scaffolds mimic the structure of the extracellular matrix of human body tissues. These scaffolds can improve cell attachment behavior and subsequent cell proliferation and differentiation. On the other hand, due to their large surface area to volume ratio and porous morphology, electrospun micro- and nanofibers are potentially useful for the controlled release of therapeutic agents (drugs and therapeutic biomolecules) in human bodies. In this study, electrospinning of poly(L-lactic acid) (PLLA) nonwoven micro- and nanofibrous membranes was investigated. It is known that the morphology and size of a drug carrier could play very important roles in the drug release behavior. Therefore, in the present investigation, a comprehensive study on the fabrication parameters that could affect the morphology and diameter of PLLA fibers was conducted. For electrospinning, several parameters were associated with intrinsic properties of the polymer solution, such as PLLA intrinsic viscosity, polymer solution concentration and solvent used, while other parameters were related to the experimental setup and electrospinning environment, including applied voltage, working distance, needle size, feeding rate, etc. Among these parameters, some solution related factors were important for controlling the fiber diameter. The average fiber diameter decreased from 3.2 μm to 0.6 μm when N,N-dimethylformamide (DMF) was added into a solvent system. While using dichloromethane (DCM) as the solvent, the fiber diameter could vary between 1 μm to 8 μm using different PLLA solution concentrations. Different solvent systems could also affect the morphology of PLLA fibers. On the other hand, most of the apparatus and environment related parameters could help to improve the fiber morphology, but not very significantly. It was also found that the stability of electrospinning conditions may improve the uniformity of PLLA fiber diameter. When lower voltage was applied, although the average fiber diameter increased, the range of variation of fiber diameters decreased. This study shows that PLLA fibrous membranes with a controllable average fiber diameter ranging from 600 nm to 8 μm could be fabricated via electrospinning. These fibrous membranes have the potential as vehicles for the controlled release of therapeutic agents in tissue engineering.

Electrospun hybrid nanofibrous meshes with adjustable performance for potential use in soft tissue engineering

2021 ◽  
pp. 004051752110639
Author(s):  
Ye Qi ◽  
Huiyuan Zhai ◽  
Yaning Sun ◽  
Hongxing Xu ◽  
Shaohua Wu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Soft Tissue ◽  
Fiber Diameter ◽  
Fiber Morphology ◽  
Surface Hydrophilicity ◽  
Nanofibrous Scaffolds ◽  
Soft Tissue Engineering ◽  
The Mean

Electrospun nanofibrous scaffolds have gained extensive attention in the fields of soft tissue engineering and regenerative medicine. In this study, a series of biodegradable nanofibrous meshes were fabricated by electrospinning poly(ε-caprolactone) (PCL) and poly( p-dioxanone) (PPDO) blends with various mass ratios. All the as-developed PCL/PPDO nanofibrous meshes possessed smooth and highly aligned fiber morphology. The mean fiber diameter was 521.5 ± 76.6 nm for PCL meshes and 485.8 ± 88.9 nm for PPDO meshes, and the mean fiber diameter seemed to present a decreasing tendency with the increasing of the PPDO component. For pure PCL meshes, the contact angle was about 117.5 ± 1.6°, the weight loss ratio was roughly 0.2% after 10 weeks of degradation, and the tensile strength was 41.2 ± 2.3 MPa in the longitudinal direction and 4.2 ± 0.1 MPa in the transverse direction. It was found that the surface hydrophilicity and in vitro degradation properties of PCL/PPDO meshes apparently increased, but the mechanical properties of PCL/PPDO meshes obviously decreased when more PPDO component was introduced. The biological tests showed that 4:1 PCL/PPDO nanofibrous meshes and 1:1 PCL/PPDO nanofibrous meshes could obviously promote the adhesion and proliferation of human adipose derived mesenchymal stem cells more than pure PCL and PPDO meshes and 1:4 PCL/PPDO meshes. The results demonstrated that it is feasible to adjust the surface hydrophilicity, degradation profile, and mechanical properties as well as biological properties of as-obtained nanofibrous meshes by blending PCL and PPDO components. This study provides meaningful reference and guidance for the design and development of PCL/PPDO hybrid nanofibrous scaffolds for soft tissue engineering research and application.

scholarly journals Structural and Morphological Characterization of Micro and Nanofibers Produced by Electrospinning and Solution Blow Spinning: A Comparative Study

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Juliano E. Oliveira ◽  
Luiz H. C. Mattoso ◽  
William J. Orts ◽  
Eliton S. Medeiros
Keyword(s):  
Morphological Characterization ◽  
Contact Angles ◽  
Fiber Formation ◽  
Poly Lactic Acid ◽  
Fiber Morphology ◽  
Xrd Diffraction ◽  
Submicron Scale ◽  
Solution Blow Spinning ◽  
Diffraction Patterns ◽  
Spun Fibers

Nonwoven mats of poly(lactic acid) (PLA), poly(ethylene oxide) (PEO), and poly(ε-caprolactone) (PCL) were prepared at a nano- and submicron scale by solution blow spinning (SBS) and electrospinning in order to compare crystalline structure and morphology developed by both processes during fiber formation. Polymer solutions were characterized by rheometry and tensiometry. Spun fibers were characterized by several analytical steps. SEM analyses showed that both solution blow spun and electrospun fibers had similar morphology. Absence of residual solvents and characteristic infrared bands in the solution blow spun fibers for PLA, PCL, and PEO was confirmed by FTIR studies. XRD diffraction patterns for solution blow spun and electrospun mats revealed some differences related to distinct mechanisms of fiber formation developed by each process. Significant differences in thermal behavior by DSC were observed between cast films of PLA, PCL, and PEO and their corresponding spun nanofibers. Furthermore, the average contact angles for spun PLA and PCL were higher than for electrospun mats, whereas it was slightly lower for PEO. When comparing electrospun and solution blow spun fibers, it was possible to verify that fiber morphology and physical properties depended both on the spinning technique and type of polymer.

Preparation of Fibrous Scaffolds Containing Calcium and Silicon Species

2011 ◽  
Vol 493-494 ◽  
pp. 840-843
Author(s):  
Akiko Obata ◽  
Hiroki Ozasa ◽  
Julian R. Jones ◽  
Toshihiro Kasuga
Keyword(s):  
Tissue Engineering ◽  
Hybrid Materials ◽  
Fiber Diameter ◽  
Fiber Spinning ◽  
High Porosity ◽  
Cotton Wool ◽  
Tissue Engineering Scaffolds ◽  
Large Defect ◽  
Nanofibrous Structure ◽  
Versatile Technique

Materials for bone defect filling should have 3D macroporous structure and be flexible to be packed into complex defects with limited entrance space. Tissue engineering scaffolds should also mimic the structure and morphology of the host tissue. Electrospinning is a versatile technique to produce materials with micro/nanofibrous structure, large surface area and high porosity. Electrospun materials are very promising for tissue engineering due to the possibility of mimicking the fibrous structure of natural extra cellular matrix (ECM). Siloxane-containing vaterite (SiV)/poly (L-lactic acid) (PLLA) hybrids (SiPVH) with controlled silicate and calcium ions releasing ability has been produced in our group. They have also demonstrated good cell infiltration into the electrospun hybrid materials that had fiber diameters greater than 10 μm. However, these electrospun hybrid materials were planar (2D) and are not suitable for large defect regeneration. In this work, the development of a fabrication technique for the production of 3D cotton wool-like structures with fiber diameter in the range of 10 μm was performed. SiPVH cotton wool-like structure containing 0, 30 and 60 wt % SiV were prepared by blowing air in the direction perpendicular to fiber spinning. Si-vaterite particles and small pores were found on the surface of the fibers. The fiber diameter of the samples were found to be in the range of 10 ~ 20 μm. Stretch tests showed more than 50 % extension for the SiPVH cotton wool-like material containing 30 wt % SiV (SiPVH30). This extension was similar to that observed for the PLLA cotton wool-like material. The results suggest that the SiPVH30 cotton wool-like material are good candidates for bone tissue engineering scaffolds.

scholarly journals Production of fibrous polymer scaffolds for tissue engineering using an automated solution blow spinning system

2018 ◽  
Vol 34 (3) ◽  
pp. 273-278 ◽  
Author(s):  
Alessandra Forgatti Hell ◽  
Márcia Mayumi Omi Simbara ◽  
Paulo Rodrigues ◽  
Danilo Akio Kakazu ◽  
Sônia Maria Malmonge
Keyword(s):  
Tissue Engineering ◽  
Polymer Scaffolds ◽  
Fibrous Polymer ◽  
Solution Blow Spinning

Electrostatic-assisted centrifugal spinning for continuous collection of submicron fibers

2016 ◽  
Vol 87 (19) ◽  
pp. 2349-2357 ◽  
Author(s):  
Huanhuan Chen ◽  
XiangLong Li ◽  
Nan Li ◽  
Bin Yang
Keyword(s):  
Fiber Diameter ◽  
Electrostatic Force ◽  
Fiber Formation ◽  
Mechanism Analysis ◽  
Strongly Correlated ◽  
Fiber Morphology ◽  
Jet Trajectory ◽  
Centrifugal Spinning ◽  
Nozzle Size ◽  
Key Factor

Non-uniformity of the fiber diameter and difficulty in continuous web collection have limited the development and further application of centrifugal spinning (CS). Here, we present a feasible method for fibers' continuous collection and morphology optimization by utilizing vertical electrostatic-assisted centrifugal spinning (E-CS). The effects of spinning parameters, such as applied voltage, nozzle size, and rotational speed on fiber morphology have been evaluated systematically. We find that vertical voltage is strongly correlated with the formation of bead defects, and nozzle size is the most important parameter on fiber size, and the fiber diameter generally decreased with increasing rotation speed. Through the mechanism analysis and jet trajectory observation, we think that the Rayleigh–Taylor instability is the key factor in determining fiber formation in CS. When a vertical electrostatic force is applied to CS, the above instability phenomenon can be effectively controlled resulting more uniform fibers with thinner diameters and fewer beads.

Solution blow spinning of PLLA/Hydroxyapatite composite scaffolds for bone tissue engineering

2021 ◽  
Author(s):  
Arnold Popkov ◽  
Denis Kulbakin ◽  
Dmitry Popkov ◽  
Elena Gorbach ◽  
Natalia Kononovich ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffolds ◽  
Hydroxyapatite Composite ◽  
Solution Blow Spinning

scholarly journals Dynamic Image Analysis of Glass Fibers as Industrial Fillers and Understanding the Influence of Processing Conditions on the Fiber Length and the Mechanical Properties of Polymers

2013 ◽  
Vol 19 (S2) ◽  
pp. 800-801
Author(s):  
P. Bajaj ◽  
C. Strom
Keyword(s):  
Mechanical Properties ◽  
Image Analysis ◽  
Fiber Length ◽  
Glass Fibers ◽  
Processing Conditions ◽  
Dynamic Image ◽  
Dynamic Image Analysis

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

A novel method for the determination of cell infiltration into nanofiber scaffolds using image analysis for tissue engineering applications

2009 ◽  
Vol 111 (1) ◽  
pp. 317-322 ◽  
Author(s):  
Dariush Semnani ◽  
Laleh Ghasemi-Mobarakeh ◽  
Mohammad Morshed ◽  
Mohammad-Hossein Nasr-Esfahani
Keyword(s):  
Tissue Engineering ◽  
Image Analysis ◽  
Cell Infiltration ◽  
Engineering Applications ◽  
Nanofiber Scaffolds ◽  
Novel Method
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