scholarly journals Secondary Caries Adjacent to Bulk or Incrementally Filled Composites Placed after Selective Excavation In Vitro

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 939
Author(s):  
Haitham Askar ◽  
Allam Al-Abdi ◽  
Uwe Blunck ◽  
Gerd Göstemeyer ◽  
Sebastian Paris ◽  
...  
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Secondary Caries ◽  
Biofilm Model ◽  
Mechanical Cycling ◽  
Carious Lesions ◽  
Deep Carious Lesions ◽  
Caries Lesions ◽  
Caries Excavation ◽  
Mineral Loss

Objectives: selective caries excavation (SE) is recommended for deep carious lesions. Bulk fill composites (BF) may be considered to restore SE-cavities. We compared the susceptibility for secondary caries adjacent to BF versus incrementally filled composites (IF) in SE and non-selectively excavated teeth (NS) in vitro. Methods: in 72 extracted human premolars, artificial caries lesions were induced on pulpo-axial walls of standardized cavities. The lesions were left (SE) or removed (NS), and teeth were restored using two BF, GrandioSO x-tra/Voco (BF-Gra) and SDR/Dentsply (BF-SDR), and an IF, GrandioSO/Voco (IF-Gra) (n = 12/group for SE and NS). After thermo-mechanical cycling (5–55 °C, 8 days), teeth were submitted to a continuous-culture Lactobacillus rhamnosus biofilm model with cyclic loading for 10 days. Mineral loss (ΔZ) of enamel surface lesions (ESL), dentin surface lesions (DSL), and dentin wall lesions (DWL) was analyzed using transversal microradiography. Results: ΔZ was the highest in DSL, followed by ESL, and it was significantly lower in DWL. There were no significant differences in ΔZ between groups in DSL, ESL, and DWL (p > 0.05). Regardless of lesion location, ΔZ did not differ between SE and NS (p > 0.05). Conclusions: BF and IF both showed low risks for DWL (i.e., true secondary caries) after SE in vitro, and surface lesion risk was also not significantly different between materials. SE did not increase secondary caries risk as compared with NS. Clinical Significance: the risk of secondary caries was low after selective excavation in this study, regardless of whether bulk or incrementally filled composites were used

Margin Integrity and Secondary Caries of Lined or Non-lined Composite and Glass Hybrid Restorations After Selective Excavation In Vitro

2017 ◽  
Vol 42 (2) ◽  
pp. 155-164 ◽  
Author(s):  
F Schwendicke ◽  
JLM Kniess ◽  
S Paris ◽  
U Blunck
Keyword(s):  
Calcium Hydroxide ◽  
Resin Composite ◽  
Lactobacillus Rhamnosus ◽  
Secondary Caries ◽  
Thermomechanical Cycling ◽  
Biofilm Model ◽  
Carious Lesions ◽  
Deep Carious Lesions ◽  
Composite Restoration

SUMMARY Objectives: For deep carious lesions, selective carious tissue removal (leaving soft dentin close to the pulp) is suggested. Afterward, different restoration materials, such as resin composites or glass hybrids (GHs), can be placed. Many dentists also apply setting or non-setting calcium hydroxide liners before restoration. We compared margin integrity and susceptibility for secondary caries in differently restored premolars in vitro. Methods: In 48 extracted human premolars, artificial residual lesions were induced on pulpo-axial walls of standardized cavities. Teeth were restored using a GH (Equia Forte) or adhesively placed resin composite restoration (OptiBond FL and Tetric EvoCeram) without any liner (RC), resin composite restoration with a non-setting calcium hydroxide liner (RC_NCH), or resin composite restoration with a setting calcium hydroxide liner (RC_SCH). After thermomechanical cycling, groups (n=12) were compared regarding their gingivocervical margin integrity (proportion of irregularities, microgaps, gaps >5 μm, overhangs). Teeth were then submitted to a continuous culture Lactobacillus rhamnosus biofilm model. After 14 days, bacterial numbers in biofilms, along tooth-restoration margins and mineral loss (ΔZ) of secondary lesions, were determined. Results: GH and RC_NCH showed significantly higher proportions of irregularities than RC and RC_SCH (p<0.05/Mann-Whitney). GH also showed significantly more gaps than alternative restorations (p<0.05). Bacterial numbers and ΔZ did not differ significantly between groups (p>0.05). Conclusions: GH and composites lined with non-setting calcium hydroxide showed reduced margin integrity compared with non-lined composites or composites lined with setting calcium hydroxide. This did not increase susceptibility for secondary caries.

Influence of biofilm removal from the tooth-restoration interface on the progression of secondary caries lesions: a preliminary in vitro model study

Biofouling ◽  
2021 ◽  
pp. 1-12
Author(s):  
Cácia Signori ◽  
Tamires Timm Maske ◽  
Vitor Henrique Digmayer Romero ◽  
Maximiliano Sérgio Cenci
Keyword(s):  
In Vitro Model ◽  
Secondary Caries ◽  
Model Study ◽  
Biofilm Removal ◽  
Vitro Model ◽  
Caries Lesions

Clinical Evaluation of Ozone on Dentinal Lesions in Young Permanent Molars using the Stepwise Excavation

2017 ◽  
Vol 41 (6) ◽  
pp. 429-441 ◽  
Author(s):  
Osama Safwat ◽  
Mona Elkateb ◽  
Karin Dowidar ◽  
Omar El Meligy
Keyword(s):  
Ozone Treatment ◽  
Group I ◽  
Carious Lesions ◽  
Deep Carious Lesions ◽  
Stepwise Excavation ◽  
Permanent Molars ◽  
Clinical Changes ◽  
And Control ◽  
Caries Excavation ◽  
Indirect Pulp Capping

Aim: To evaluate the clinical changes in dentin of deep carious lesions in young permanent molars, following ozone application with and without the use of a remineralizing solution, using the stepwise excavation. Study design: The sample included 162 first permanent immature molars, showing deep occlusal carious cavities that were indicated for indirect pulp capping. Teeth were divided into 2 main groups according to the method of ozone treatment. Each group was further subdivided equally into test and control subgroups. Following caries excavation, color, consistency and DIAGNOdent assessments of dentin were evaluated after 6 and 12 months. Results: Regarding dentin color and consistency, no significant differences were observed following ozone application, with and without a remineralizing solution. There were no significant differences between ozone treatment, and calcium hydroxide during the different evaluation periods, except in group I cases after 6 months, concerning the dentin color. The DIAGNOdent values were significantly reduced following ozone application, with or without a remineralizing solution, as well as between test and control cases in group I after 6 months. Conclusions: Ozone application through the stepwise excavation had no significant effect on dentin color and consistency in young permanent molars. DIAGNOdent was unreliable in monitoring caries activity.

scholarly journals Interactions between Lactobacillus rhamnosus GG and oral micro-organisms in an in vitro biofilm model

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Qingru Jiang ◽  
Iva Stamatova ◽  
Veera Kainulainen ◽  
Riitta Korpela ◽  
Jukka H. Meurman
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Lactobacillus Rhamnosus Gg ◽  
Biofilm Model ◽  
Micro Organisms

Randomized Clinical Trial to Evaluate MTA Indirect Pulp Capping in Deep Caries Lesions After 24-Months

2017 ◽  
Vol 42 (5) ◽  
pp. 470-477 ◽  
Author(s):  
U Koc Vural ◽  
A Kiremitci ◽  
S Gokalp
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Fisher Exact Test ◽  
Exact Test ◽  
Pulp Capping ◽  
Permanent Molar ◽  
Carious Lesions ◽  
Deep Carious Lesions ◽  
Caries Removal ◽  
Removal Technique ◽  
Caries Lesions

SUMMARY Objective: This clinical study aimed to assess the efficacies of mineral trioxide aggregate (MTA) and calcium hydroxide [Ca(OH)2] in the treatment of deep carious lesions by the direct complete caries removal technique. Methods and Materials: A total of 100 permanent molar/premolar teeth were capped with either Ca(OH)2 (n=49) or MTA (n=51) and restored with composite resin in 73 patients. Periapical radiographs were acquired prior to the treatment as well as at six, 12, and 24 months posttreatment. Two calibrated examiners performed the clinical and radiographic assessment of the periapical pathology and pulpal symptoms. Intergroup comparisons of the observed values were performed using the Fisher exact test. Significance was predetermined at α = 0.05. Results: The recall rates were 100% at six and 12 months posttreatment and 98.6% at 24 months posttreatment. Four teeth capped with Ca(OH)2 (two each at six and 12 months posttreatment) and two capped with MTA (one each at 12 and 24 months posttreatment) received endodontic emergency treatment because of symptoms of irreversible pulpitis, which were clinically and/or radiographically established. There were no significant differences in pulp vitality between the two pulp-capping agents at six, 12, or 24 months posttreatment (p=0.238, p=0.606, and p=0.427, respectively). Conclusions: Both pulp-capping materials were found to be clinically acceptable at 24 months posttreatment.

Effect of the Probiotic Lactobacillus rhamnosus LB21 on the Cariogenicity of Streptococcus mutans UA159 in a Dual-Species Biofilm Model

2015 ◽  
Vol 49 (6) ◽  
pp. 583-590 ◽  
Author(s):  
Constanza E. Fernández ◽  
Rodrigo A. Giacaman ◽  
Livia M. Tenuta ◽  
Jaime A. Cury
Keyword(s):  
Streptococcus Mutans ◽  
Culture Media ◽  
Lactobacillus Rhamnosus ◽  
Surface Hardness ◽  
Single Species ◽  
Initial Inoculum ◽  
Biofilm Model ◽  
Caries Model ◽  
Bovine Enamel

Despite promising results using probiotics, evidence of the preventive effect on enamel demineralization is insufficient and the cariogenic potential of probiotics is still controversial. Probiotics could affect biofilm formation and interfere with adherence, growth or coaggregation with Streptococcus mutans in biofilms. However, most of the studies have been conducted using planktonic bacteria. Hence, the aim of the study was to assess the effect of probiotic bacteria on the cariogenicity of S. mutans using an in vitro biofilm caries model on enamel. Single-species biofilms (S. mutans UA159, SM or Lactobacillus rhamnosus LB21, LB) or dual-species biofilms simultaneously inoculated (SM + LB) or LB inoculated 8 h after SM (SM → LB) were grown for 96 h. Biofilms were formed on bovine enamel saliva-coated slabs of known surface hardness (SH) and immersed in culture media. Biofilms were exposed 8 times per day to 10% sucrose. Medium pH was monitored twice daily as a biofilm acidogenicity indicator. After 96 h, biofilms were collected to determine biomass and bacteria viability. Slab demineralization was calculated as percentage of SH loss (%SHL). Additionally, the model was tested with different concentrations of the initial inoculum (103, 106, 108 cells/ml) and different adhesion times (2 or 8 h). The dual-species biofilm revealed no LB effects on SM cariogenicity, without changes in acidogenicity or %SHL among groups (p > 0.05, n = 12). Lack of activity of LB on SM cariogenicity persisted even when 105 times higher concentration of the probiotic was tested. Coaggregation was not observed. In conclusion, findings suggest that LB does not reduce cariogenicity of SM in a validated experimental caries model.

scholarly journals Attitudes And Behavior Of Dentists Regarding Management Of Deep Carious Lesion

2019 ◽  
Vol 09 (03) ◽  
pp. 206-209
Author(s):  
Umeed Javaid ◽  
Shama Asghar ◽  
Kulsoom Fatima Rizvi
Keyword(s):  
Cross Sectional ◽  
Academic Qualification ◽  
Carious Lesions ◽  
Clinical Scenarios ◽  
Deep Carious Lesions ◽  
Deep Carious Lesion ◽  
Caries Removal ◽  
And Behavior ◽  
Attitudes And Behavior ◽  
Caries Excavation

Objective: To assess the attitude and behavior of dentists for the management of deep carious lesions. Study Design and Setting: It was a cross sectional based study conducted at six dental institutes of Karachi from both government and private sectors. Methodology: The included participants were the dental graduates and post graduates working in different specialties of dentistry. Whereas graduates and post graduates not affiliated with any institution were excluded from the study. The Questionnaire comprises of two parts, first part was composed of participant’s demographic details and academic qualification, while the second part comprised of clinical scenarios regarding management of deep carious lesions. Results: From the 250 distributed questionnaires, 218 dentists returned the questionnaire giving a satisfactory response rate of 87.2%. There were 28% male and 72%% female dentists. The majority of dentists were aged between 25-35 years old. Complete caries removal was the management of choice by 72.5% of dentists for deep carious lesions. Conclusion: Complete caries removal was the most preferred treatment modality when the risk of dental pulpal exposure associated with caries excavation is low. However when the risk is high, step-wise excavation was preferred procedure by half of the participants.

scholarly journals Restoration Materials and Secondary Caries Using an In Vitro Biofilm Model

2014 ◽  
Vol 94 (1) ◽  
pp. 62-68 ◽  
Author(s):  
N.K. Kuper ◽  
F.H. van de Sande ◽  
N.J.M. Opdam ◽  
E.M. Bronkhorst ◽  
J.J. de Soet ◽  
...  
Keyword(s):  
Secondary Caries ◽  
Biofilm Model
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