scholarly journals A GelMA-PEGDA-nHA Composite Hydrogel for Bone Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 3735
Author(s):  
Yihu Wang ◽  
Xiaofeng Cao ◽  
Ming Ma ◽  
Weipeng Lu ◽  
Bing Zhang ◽  
...  
Keyword(s):  
Bone Repair ◽  
Composite Hydrogel ◽  
Hydroxyl Group ◽  
Poly Ethylene Glycol ◽  
Hydrogel Scaffold ◽  
Glycol Diacrylate ◽  
3D Printed ◽  
Poly Ethylene ◽  
The Stability

A new gelatin methacrylamine (GelMA)-poly (ethylene glycol) diacrylate (PEGDA)-nano hydroxyapatite (nHA) composite hydrogel scaffold was developed using UV photo-crosslinking technology. The Ca2+ from nHA can form a [HO]Ca2+ [OH] bridging structure with the hydroxyl group in GelMA, thereby enhancing the stability. Compared with GelMA-PEGDA hydrogel, the addition of nHA can control the mechanical properties of the composite hydrogel and reduce the degradation rate. In vitro cell culture showed that osteoblast can adhere and proliferate on the surface of the hydrogel, indicating that the GelMA-PEGDA-nHA hydrogel had good cell viability and biocompatibility. Furthermore, GelMA-PEGDA-nHA has excellent injectability and rapid prototyping properties and is a promising 3D printed bone repair scaffold material.

scholarly journals A Non-Cytotoxic Resin for Micro-Stereolithography for Cell Cultures of HUVECs

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 246 ◽  
Author(s):  
Max Männel ◽  
Carolin Fischer ◽  
Julian Thiele
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Umbilical Vein ◽  
Polymer Materials ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
3D Printed ◽  
Poly Ethylene ◽  
The Impact

Three-dimensional (3D) printing of microfluidic devices continuously replaces conventional fabrication methods. A versatile tool for achieving microscopic feature sizes and short process times is micro-stereolithography (µSL). However, common resins for µSL lack biocompatibility and are cytotoxic. This work focuses on developing new photo-curable resins as a basis for µSL fabrication of polymer materials and surfaces for cell culture. Different acrylate- and methacrylate-based compositions are screened for material characteristics including wettability, surface roughness, and swelling behavior. For further understanding, the impact of photo-absorber and photo-initiator on the cytotoxicity of 3D-printed substrates is studied. Cell culture experiments with human umbilical vein endothelial cells (HUVECs) in standard polystyrene vessels are compared to 3D-printed parts made from our library of homemade resins. Among these, after optimizing material composition and post-processing, we identify selected mixtures of poly(ethylene glycol) diacrylate (PEGDA) and poly(ethylene glycol) methyl ethyl methacrylate (PEGMEMA) as most suitable to allow for fabricating cell culture platforms that retain both the viability and proliferation of HUVECs. Next, our PEGDA/PEGMEMA resins will be further optimized regarding minimal feature size and cell adhesion to fabricate microscopic (microfluidic) cell culture platforms, e.g., for studying vascularization of HUVECs in vitro.

In Vitro and in Vivo Performance of Porcine Islets Encapsulated in Interfacially Photopolymerized Poly(Ethylene Glycol) Diacrylate Membranes

1999 ◽  
Vol 8 (3) ◽  
pp. 293-306 ◽  
Author(s):  
Gregory M. Cruise ◽  
Orion D. Hegre ◽  
Francis V. Lamberti ◽  
Steven R. Hager ◽  
Ron Hill ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
Porcine Islets ◽  
Poly Ethylene

scholarly journals Synthesis and Characterization of Novel Copolymeric Resveratrol Conjugates

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Yan-Jing Ng ◽  
Heather A. E. Benson ◽  
David H. Brown ◽  
Yan Chen
Keyword(s):  
Micelle Formation ◽  
Rat Plasma ◽  
The Body ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
The Stability ◽  
Chain Lengths ◽  
Cardioprotective Drug

Resveratrol (RSV), naturally found in plants, is known to have health benefits and has been proposed as a potential anticancer and cardioprotective drug. However, due to its molecular structure, it undergoes rapid metabolism in the body resulting in low bioavailability. Novel polymeric methoxy-poly(ethylene glycol)-block-poly(ε-caprolactone) (mPEG-PCL) RSV conjugates with varying PCL chain lengths have been synthesised and formulated into micelles and/or nanoparticles for preliminaryin vitrostability studies. RSV conjugated with mPEG2000-PCL9500 was found to have improved solubility and stability of RSV as compared to RSV alone. The length of the PCL chain was found to affect the micelle formation, hence the stability in physiological buffers and rat plasma.

scholarly journals Hydrogel co-networks of gelatine methacrylate and poly(ethylene glycol) diacrylate sustain 3D functional in vitro models of intestinal mucosa

2020 ◽  
Vol 12 (2) ◽  
pp. 025008 ◽  
Author(s):  
Anna Vila ◽  
Núria Torras ◽  
Albert G Castaño ◽  
María García-Díaz ◽  
Jordi Comelles ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Intestinal Mucosa ◽  
In Vitro Models ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
Poly Ethylene

scholarly journals Influence of Poly(Ethylene Glycol) End Groups on Poly(Ethylene Glycol)-Albumin System Properties as a Potential Degradable Tissue Scaffold

2018 ◽  
Vol 10 (1) ◽  
pp. 1 ◽  
Author(s):  
Robyn Overby ◽  
Dale Feldman
Keyword(s):  
Ethylene Glycol ◽  
Crosslinking Agent ◽  
Poly Ethylene Glycol ◽  
In Vivo Evaluation ◽  
Tissue Scaffold ◽  
Handling Characteristics ◽  
Poly Ethylene ◽  
The Stability

Chronic dermal lesions, such as pressure ulcers, are difficult to heal. Degradable tissue scaffold systems can be employed to serve as a provisional matrix for cellular ingrowth and facilitate regenerative healing during degradation. Degradable regenerative tissue scaffold matrices can be created by crosslinking albumin with functionalized poly(ethylene glycol) (PEG) polymers. The purpose of this study was to evaluate the stability of PEG-albumin scaffold systems formed using PEG polymers with three different functionalized end chemistries by quantifying in vitro system swellability to determine the most promising PEG crosslinking polymer for wound healing applications. Of the three polymers evaluated, PEG-succinimidyl glutarate (SG) exhibited consistent gelation and handling characteristics when used as the crosslinking agent with albumin. PEG-SG polymers were identified as an appropriate synthetic crosslinking moiety in a PEG-albumin scaffold system, and further in vitro and in vivo evaluation of this scaffold system is merited.

Three-Dimensional Scaffold Fabrication with Inverse Photolithography

MRS Advances ◽  
2016 ◽  
Vol 2 (19-20) ◽  
pp. 1071-1075 ◽  
Author(s):  
Ramesh Prashad ◽  
Ozlem Yasar
Keyword(s):  
Interior Design ◽  
Uv Light ◽  
Three Dimensional ◽  
Poly Ethylene Glycol ◽  
Scaffold Fabrication ◽  
Glycol Diacrylate ◽  
Alternative Approach ◽  
Cell Sources ◽  
3D Printed ◽  
Poly Ethylene

ABSTRACTIn recent years, tissue engineering has been utilized as an alternative approach to organ transplantation. Success rate of tissue regeneration influenced by the biomaterials, cell sources, growth factors and scaffold fabrication. Design and precise fabrication of scaffolds are required to support cells to expand and migrate to 3D environment. Common scaffold fabrication techniques use heat, adhesives, molds or light. In this research, “inverse-photolithography” which is a light based fabrication technique was used to generate the scaffolds. In order to control the interior architecture of the scaffold “a single vertical strut” and “a y-shape” were fabricated with the 3D printer by using the dissolvable filament. Then, the strut and the y-shape were immersed into the photo-curable solution which is poly(ethylene glycol) diacrylate (PEGDA) and photo-initiator mixture. UV light with the 365nm wavelength was placed up-side down under the solution. Photo-curable mixture was exposed to the UV light for 3 minutes to cure the entire scaffold. Solidified scaffold with the strut and y-shape inside was kept in the limonene solution. Limonene penetrated through the open ended strut and y-shape and it dissolved the 3D printed strut and y-shape away leaving the fabricated PEGDA based scaffolds. This preliminary research showcases, the 3D scaffolds with the controlled interior design, can be fabricated with the “inverse-photolithography” technique.

scholarly journals On-demand modulation of 3D-printed elastomers using programmable droplet inclusions

2020 ◽  
Vol 117 (26) ◽  
pp. 14790-14797 ◽  
Author(s):  
Hing Jii Mea ◽  
Luis Delgadillo ◽  
Jiandi Wan
Keyword(s):  
Three Dimensional ◽  
Continuous Phase ◽  
Direct Writing ◽  
Poly Ethylene Glycol ◽  
Glass Capillary ◽  
Glycol Diacrylate ◽  
Dynamic Tuning ◽  
3D Printed ◽  
Poly Ethylene

One of the key thrusts in three-dimensional (3D) printing and direct writing is to seamlessly vary composition and functional properties in printed constructs. Most inks used for extrusion-based printing, however, are compositionally static and available approaches for dynamic tuning of ink composition remain few. Here, we present an approach to modulate extruded inks at the point of print, using droplet inclusions. Using a glass capillary microfluidic device as the printhead, we dispersed droplets in a polydimethylsiloxane (PDMS) continuous phase and subsequently 3D printed the resulting emulsion into a variety of structures. The mechanical characteristics of the 3D-printed constructs can be tuned in situ by varying the spatial distribution of droplets, including aqueous and liquid metal droplets. In particular, we report the use of poly(ethylene glycol) diacrylate (PEGDA) aqueous droplets for local PDMS chemistry alteration resulting in significant softening (85% reduced elastic modulus) of the 3D-printed constructs. Furthermore, we imparted magnetic functionality in PDMS by dispersing ferrofluid droplets and rationally designed and printed a rudimentary magnetically responsive soft robotic actuator as a functional demonstration of our droplet-based strategy. Our approach represents a continuing trend of adapting microfluidic technology and principles for developing the next generation of additive manufacturing technology.

Sign in / Sign up

Export Citation Format

Share Document