scholarly journals Adipose Tissue-Derived Stem Cells: The Biologic Basis and Future Directions for Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3210
Author(s):  
Diana Câmara ◽  
Jamil Shibli ◽  
Eduardo Müller ◽  
Paulo De-Sá-Junior ◽  
Allan Porcacchia ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Human Leukocyte ◽  
Basic Research ◽  
Telomerase Activity ◽  
Adipose Derived Stem Cells ◽  
Precise Control ◽  
Leukocyte Antigens ◽  
Active Research

Mesenchymal stem cells (MSCs) have been isolated from a variety of tissues using different methods. Active research have confirmed that the most accessible site to collect them is the adipose tissue; which has a significantly higher concentration of MSCs. Moreover; harvesting from adipose tissue is less invasive; there are no ethical limitations and a lower risk of severe complications. These adipose-derived stem cells (ASCs) are also able to increase at higher rates and showing telomerase activity, which acts by maintaining the DNA stability during cell divisions. Adipose-derived stem cells secret molecules that show important function in other cells vitality and mechanisms associated with the immune system, central nervous system, the heart and several muscles. They release cytokines involved in pro/anti-inflammatory, angiogenic and hematopoietic processes. Adipose-derived stem cells also have immunosuppressive properties and have been reported to be “immune privileged” since they show negative or low expression of human leukocyte antigens. Translational medicine and basic research projects can take advantage of bioprinting. This technology allows precise control for both scaffolds and cells. The properties of cell adhesion, migration, maturation, proliferation, mimicry of cell microenvironment, and differentiation should be promoted by the printed biomaterial used in tissue engineering. Self-renewal and potency are presented by MSCs, which implies in an open-source for 3D bioprinting and regenerative medicine. Considering these features and necessities, ASCs can be applied in the designing of tissue engineering products. Understanding the heterogeneity of ASCs and optimizing their properties can contribute to making the best therapeutic use of these cells and opening new paths to make tissue engineering even more useful.

Adipose tissue engineering with naturally derived scaffolds and adipose-derived stem cells

Biomaterials ◽  
2007 ◽  
Vol 28 (26) ◽  
pp. 3834-3842 ◽  
Author(s):  
Lauren Flynn ◽  
Glenn D. Prestwich ◽  
John L. Semple ◽  
Kimberly A. Woodhouse
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Adipose Derived Stem Cells ◽  
Adipose Tissue Engineering

scholarly journals Adipose-derived stem cells for tissue engineering and therapy of non-healing wounds

2018 ◽  
Vol 72 ◽  
pp. 806-821 ◽  
Author(s):  
Adriana Schumacher ◽  
Mirosława Cichorek ◽  
Michał Pikuła
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Clinical Trials ◽  
Adipose Tissue ◽  
Growth Factors ◽  
Chronic Wounds ◽  
Clinical Effects ◽  
Adipose Derived Stem Cells ◽  
Healing Wounds

Chronic wounds seem to be a big problem for the medicinal, social and commercial area, especially for elder patients or people with cancer, metabolic or autoimmune diseases. In this respect, in the modern regenerative medicine there are intensive studies on methodologies that stimulate healing of chronic wounds (diabetic foots, ulcers, burns). In tissue engineering new solutions in wound healing are based on cellular therapies which consisting of growth factors and various types of scaffolds. In this way, there are created skin substitutes which are composed of cellular auto/allografts (stem cells and differentiated cells) and most commonly biodegradable scaffolds; they aim is not only to fill the tissue but also to stimulate wound healing. In this article we demonstrate the current knowledge about biological properties of Adipose- -derived Stem Cells (ASCs), methods of their isolation and potential for use in therapies for non-healing wounds. Adipose tissue seems to be an attractive and abundant stem cells source with therapeutic applicability in diverse phase of the repair and regeneration of the chronically damaged tissues. Additionally, it is believed that secreted by ASCs growth factors, cytokines and exosomes are decisive in the clinical effects. In this review, we also present the current clinical trials using stem cells derived from adipose tissue. Increasingly, the use of cell therapy in wound healing treatment draws attention to the safety, reproducibility and quality of stem cells. Researches go on and therapy approaches are possible but the detailed knowledge of the ASCs biology must be thoroughly investigated before these cells would be widely used in the clinical trials.

Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells

Biomaterials ◽  
2014 ◽  
Vol 35 (6) ◽  
pp. 1914-1923 ◽  
Author(s):  
Hoi Ki Cheung ◽  
Tim Tian Y. Han ◽  
Dale M. Marecak ◽  
John F. Watkins ◽  
Brian G. Amsden ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Soft Tissue ◽  
Composite Hydrogel ◽  
Adipose Derived Stem Cells ◽  
Hydrogel Scaffolds ◽  
Soft Tissue Engineering

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

Tendon Tissue Engineering: Mechanism and Effects of Human Tenocyte Coculture With Adipose-Derived Stem Cells

2018 ◽  
Vol 43 (2) ◽  
pp. 183.e1-183.e9 ◽  
Author(s):  
Chao Long ◽  
Zhen Wang ◽  
Anais Legrand ◽  
Arhana Chattopadhyay ◽  
James Chang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Tendon Tissue ◽  
Tendon Tissue Engineering

scholarly journals Epithelial differentiation of adipose-derived stem cells for laryngeal tissue engineering

2009 ◽  
Vol 120 (1) ◽  
pp. 125-131 ◽  
Author(s):  
Jennifer L. Long ◽  
Patricia Zuk ◽  
Gerald S. Berke ◽  
Dinesh K. Chhetri
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Epithelial Differentiation ◽  
Adipose Derived Stem Cells
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