scholarly journals 3D Printing of Cell Culture Devices: Assessment and Prevention of the Cytotoxicity of Photopolymers for Stereolithography

Materials ◽  
2020 ◽  
Vol 13 (13) ◽  
pp. 3011 ◽  
Author(s):  
Sebastian Kreß ◽  
Roland Schaller-Ammann ◽  
Jürgen Feiel ◽  
Joachim Priedl ◽  
Cornelia Kasper ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Rapid Prototyping ◽  
Human Mesenchymal Stem Cells ◽  
Toxic Effects ◽  
Medical Applications ◽  
Viable Option ◽  
Cytotoxic Effects ◽  
Inert Polymer ◽  
Rapid Generation

3D printing is increasingly important for the rapid prototyping of advanced and tailor-made cell culture devices. In this context, stereolithography represents a method for the rapid generation of prototypes from photocurable polymers. However, the biocompatibility of commercially available photopolymers is largely unknown. Therefore, we evaluated the cytotoxicity of six polymers, two of them certified as biocompatible according to ISO 10993-5:2009, and we evaluated, if coating with Parylene, an inert polymer widely used in medical applications, might shield cells from the cytotoxic effects of a toxic polymer. In addition, we evaluated the processability, reliability, and consistency of the details printed. Human mesenchymal stem cells (MSCs) were used for cytotoxicity testing as they are widely used and promising for numerous applications in regenerative medicine. MSCs were incubated together with printed photopolymers, and the cytotoxicity was assessed. All photopolymers significantly reduced the viability of MSCs while the officially biocompatible resins displayed minor toxic effects. Further, coating with Parylene completely protected MSCs from toxic effects. In conclusion, none of the tested polymers can be fully recommended for rapid prototyping of cell culture devices. However, coating with Parylene can shield cells from toxic effects and thus might represent a viable option until more compatible materials are available.

scholarly journals 3D printing in cell culture systems and medical applications

2018 ◽  
Vol 5 (4) ◽  
pp. 041109 ◽  
Author(s):  
Max J. Lerman ◽  
Josephine Lembong ◽  
Greg Gillen ◽  
John P. Fisher
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Medical Applications ◽  
Culture Systems ◽  
Cell Culture Systems

scholarly journals Rapid Prototyping of Cell Culture Microdevices Using Parylene-Coated 3D Prints

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Brian O'Grady ◽  
Michael Geuy ◽  
Hyosung Kim ◽  
Kylie Balotin ◽  
Everett Allchin ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Rapid Prototyping ◽  
Microfluidic Devices ◽  
Cost Effective ◽  
Specialized Training ◽  
Complex Features

Fabrication of microfluidic devices by photolithography generally requires specialized training and access to a cleanroom. As an alternative, 3D printing enables cost-effective fabrication of microdevices with complex features that would...

scholarly journals Cultivation of hMSCs in Human Plasma Prevents the Cytotoxic and Genotoxic Potential of ZnO-NP In Vitro

2019 ◽  
Vol 9 (23) ◽  
pp. 4994
Author(s):  
Scherzad ◽  
Meyer ◽  
Ickrath ◽  
Gehrke ◽  
Bregenzer ◽  
...  
Keyword(s):  
Cell Culture ◽  
Human Plasma ◽  
Calf Serum ◽  
Human Mesenchymal Stem Cells ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Zno Nps ◽  
Cytotoxic Effects ◽  
Differentiation Capacity

Zinc oxide nanoparticles (ZnO-NPs) are commonly used for industrial applications. Consequently, there is increasing exposure of humans to them. The in vitro analysis of cytotoxicity and genotoxicity is commonly performed under standard cell culture conditions. Thus, the question arises of how the results of genotoxicity and cytotoxicity experiments would alter if human plasma was used instead of cell culture medium containing of fetal calf serum (FCS). Human mesenchymal stem cells (hMSCs) were cultured in human plasma and exposed to ZnO-NPs. A cultivation in expansion medium made of DMEM consisting 10% FCS (DMEM-EM) served as control. Genotoxic and cytotoxic effects were evaluated with the comet and MTT assay, respectively. hMSC differentiation capacity and ZnO-NP disposition were evaluated by histology and transmission electron microscopy (TEM). The protein concentration and the amount of soluble Zn2+ were measured. The cultivation of hMSCs in plasma leads to an attenuation of genotoxic and cytotoxic effects of ZnO-NPs compared to control. The differentiation capacity of hMSCs was not altered. The TEM showed ZnO-NP persistence in cytoplasm in both groups. The concentrations of protein and Zn2+ were higher in plasma than in DMEM-EM. In conclusion, the cultivation of hMSCs in plasma compared to DMEM-EM leads to an attenuation of cytotoxicity and genotoxicity in vitro.

scholarly journals Identification of Novel FNIN2 and FNIN3 Fibronectin-Derived Peptides That Promote Cell Adhesion, Proliferation and Differentiation in Primary Cells and Stem Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3042
Author(s):  
Eun Ju Lee ◽  
Khurshid Ahmad ◽  
Shiva Pathak ◽  
SunJu Lee ◽  
Mohammad Hassan Baig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cell Adhesion ◽  
3D Culture ◽  
Human Mesenchymal Stem Cells ◽  
Cell Types ◽  
Primary Cells ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Promote Cell Adhesion

In recent years, a major rise in the demand for biotherapeutic drugs has centered on enhancing the quality and efficacy of cell culture and developing new cell culture techniques. Here, we report fibronectin (FN) derived, novel peptides fibronectin-based intergrin binding peptide (FNIN)2 (18-mer) and FNIN3 (20-mer) which promote cell adhesion proliferation, and the differentiation of primary cells and stem cells. FNIN2 and 3 were designed based on the in silico interaction studies between FN and its receptors (integrin α5β1, αvβ3, and αIIbβ3). Analysis of the proliferation of seventeen-cell types showed that the effects of FNINs depend on their concentration and the existence of expressed integrins. Significant rhodamine-labeled FNIN2 fluorescence on the membranes of HeLa, HepG2, A498, and Du145 cells confirmed physical binding. Double coating with FNIN2 or 3 after polymerized dopamine (pDa) or polymerized tannic acid (pTA) precoating increased HBEpIC cell proliferation by 30–40 percent, suggesting FNINs potently affect primary cells. Furthermore, the proliferation of C2C12 myoblasts and human mesenchymal stem cells (MSCs) treated with FNINs was significantly increased in 2D/3D culture. FNINs also promoted MSC differentiation into osteoblasts. The results of this study offer a new approach to the production of core materials (e.g., cell culture medium components, scaffolds) for cell culture.

Cytotoxic Effects of Benzbromarone and Its 1′-Hydroxy Metabolite in Human Hepatocarcinoma FLC4 Cells Cultured on Micro-space Cell Culture Plates

2013 ◽  
Vol 28 (3) ◽  
pp. 265-268 ◽  
Author(s):  
Kaoru Kobayashi ◽  
Eri Kajiwara ◽  
Masayuki Ishikawa ◽  
Hanaka Mimura ◽  
Hidenobu Oka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cytotoxic Effects ◽  
Cells Cultured ◽  
Space Cell ◽  
Hydroxy Metabolite

Antifungal, Anti-Inflammatory and Cytotoxic Effects of Zingiber cassumunar Gel

2018 ◽  
Vol 773 ◽  
pp. 360-364 ◽  
Author(s):  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon ◽  
Rattiporn Kaypetch
Keyword(s):  
Cell Culture ◽  
Antifungal Activity ◽  
Diffusion Method ◽  
Cytotoxic Effects ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Zingiber Cassumunar ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

This study aimed to investigate the antifungal, anti-inflammatory and cytotoxic effects of Zingiber cassumunar gel. The gel was prepared from essential oil of Zingiber cassumunar rhizome by the Thailand Institute of Scientific and Technological Research. Antifungal activity of the gel was firstly determined by the well diffusion method against Candida albicans ATCC 10238 and candida strain isolated from the patient’s lesion. Then, the Agar overlay technique was used to test the cytotoxicity of Z. cassumunar gel on mouse fibroblasts (ATCC clone 929) according to ISO 7405. For anti-inflammatory effect of the gel, TPA (carrageenan lambda type IV, 12-O-tetradecanoylphorbol-13- acetate)-induced mouse ear edema method was used. The results of well diffusion showed that Z. cassumunar gel was quite a potent antifungal agent against both strains of tested C. albicans with inhibition zones of 12-13 mm. In the cytotoxicity test, the gel exhibited no toxicity to cell culture. In addition, topical administration of Z. cassumunar gel could decrease mouse ear edema induced by TPA. At 30 and 60 min-time points, Z. cassumunar gel showed higher anti-inflammatory activity than triamcinolone which was used as reference anti-inflammatory drug. In conclusion, gel prepared from Z. cassumunar oil showed antifungal activity against both strains of C. albicans. In addition, its anti-inflammatory effect was demonstrated within 30 min by the TPA-induced mouse ear edema model. The gel was non-toxic to cell culture after 24-h incubation. Further studies are needed to clarify the safety and benefit of this gel for clinical use in the treatment of candidal infection and inflammation.

scholarly journals Novel low shear 3D bioreactor for high purity mesenchymal stem cell production

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252575
Author(s):  
Andrew B. Burns ◽  
Corinna Doris ◽  
Kevin Vehar ◽  
Vinit Saxena ◽  
Cameron Bardliving ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Stem Cell ◽  
High Purity ◽  
Culture Media ◽  
Human Mesenchymal Stem Cells ◽  
Three Dimensions ◽  
Bioreactor Culture ◽  
Differentiation Potential ◽  
Cell Culture Systems

Bone marrow derived human Mesenchymal Stem Cells (hMSCs) are an attractive candidate for regenerative medicine. However, their harvest can be invasive, painful, and expensive, making it difficult to supply the enormous amount of pure hMSCs needed for future allogeneic therapies. Because of this, a robust method of scaled bioreactor culture must be designed to supply the need for high purity, high density hMSC yields. Here we test a scaled down model of a novel bioreactor consisting of an unsubmerged 3D printed Polylactic Acid (PLA) lattice matrix wetted by culture media. The growth matrix is uniform, replicable, and biocompatible, enabling homogenous cell culture in three dimensions. The goal of this study was to prove that hMSCs would culture well in this novel bioreactor design. The system tested resulted in comparable stem cell yields to other cell culture systems using bone marrow derived hMSCs, while maintaining viability (96.54% ±2.82), high purity (>98% expression of combined positive markers), and differentiation potential.

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