scholarly journals The Use of ESEM-EDX as an Innovative Tool to Analyze the Mineral Structure of Peri-Implant Human Bone

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1671 ◽  
Author(s):  
Carlo Prati ◽  
Fausto Zamparini ◽  
Daniele Botticelli ◽  
Mauro Ferri ◽  
Daichi Yonezawa ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Bone Tissue ◽  
Bone Mineralization ◽  
Region Of Interest ◽  
Environmental Scanning ◽  
Bone Area ◽  
Bone Implant ◽  
Inorganic Content ◽  
Innovative Tool ◽  
Edx Microanalysis

This study aimed to investigate the mineralization and chemical composition of the bone–implant interface and peri-implant tissues on human histological samples using an environmental scanning electron microscope as well as energy-dispersive x-ray spectroscopy (ESEM-EDX) as an innovative method. Eight unloaded implants with marginal bone tissue were retrieved after four months from eight patients and were histologically processed and analyzed. Histological samples were observed under optical microscopy (OM) to identify the microarchitecture of the sample and bone morphology. Then, all samples were observed under ESEM-EDX from the coronal to the most apical portion of the implant at 500x magnification. A region of interest with bone tissue of size 750 × 500 microns was selected to correspond to the first coronal and the last apical thread (ROI). EDX microanalysis was used to assess the elemental composition of the bone tissue along the thread interface and the ROI. Atomic percentages of Ca, P, N, and Ti, and the Ca/N, P/N and Ca/P ratios were measured in the ROI. Four major bone mineralization areas were identified based on the different chemical composition and ratios of the ROI. Area 1: A well-defined area with low Ca/N, P/N, and Ca/P was identified as low-density bone. Area 2: A defined area with higher Ca/N, P/N, and Ca/P, identified as new bone tissue, or bone remodeling areas. Area 3: A well-defined area with high Ca/N, /P/N, and Ca/P ratios, identified as bone tissue or bone chips. Area 4: An area with high Ca/N, P/N, and Ca/P ratios, which was identified as mature old cortical bone. Bone Area 2 was the most represented area along the bone–implant interface, while Bone Area 4 was identified only at sites approximately 1.5 mm from the interface. All areas were identified around implant biopsies, creating a mosaic-shaped distribution with well-defined borders. ESEM-EDX in combination with OM allowed to perform a microchemical analysis and offered new important information on the organic and inorganic content of the bone tissue around implants.

Mineral composition and gemological characteristics of the fossil marine reptiles of the Ulyanovsk region

2018 ◽  
pp. 12-16 ◽  
Author(s):  
D. A. Petrochenkov
Keyword(s):  
Chemical Composition ◽  
Bone Tissue ◽  
Mineral Composition ◽  
Bone Structure ◽  
Upper Jurassic ◽  
Marine Reptiles ◽  
Ulyanovsk Region ◽  
Impurity Elements ◽  
Wide Assortment

Fossils of marine reptiles are a new jewelry and ornamental material and collected in the Ulyanovsk region from the Upper Jurassic deposits. They consist of (wt. %): calcite — 52, apatite — 24 and pyrite — 23, and also gypsum presents. The contents of radioactive and carcinogenic elements are close to background. The original bone structure of reptiles is preserved. Apatite replaces the bone tissue of marine reptiles, forming a cellular framework. According to the chemical composition, apatite refers to fluorohydroxyapatite with an increased Sr content. The size of the crystals is finely-dispersed. Calcite and pyrite fill the central parts of the cells. Calcite crystals of isometric and elongated shape, 0,01—0,05 mm in size, form blocks up to 0,3 mm during intergrowth. Calcite fills thin, discontinuous veins along the contour of cells with a width of up to 0,03 mm. In calcite, among the impurity elements, there are (wt. %, on the average): Mg — 0,30, Mn — 0,39 and Fe — 0,96. Pyrite forms a dispersed impregnation in calcite and apatite, content of impurities is, wt. %: Ni — up to 0,96 and Cu — up to 0,24. On technological and decorative characteristics of fossils of sea reptiles of Ulyanovsk region are qualitative jewelry and ornamental materials of biomineral group, allowing to make a wide assortment of jewelry and souvenir products.

Evaluation of Bone Ingrowth in Free Form Fabricated Scaffolds

2007 ◽  
Vol 361-363 ◽  
pp. 919-922
Author(s):  
Erik Adolfsson ◽  
Johan Malmström ◽  
Peter Thomsen
Keyword(s):  
Bone Tissue ◽  
Sintered Density ◽  
Bone Ingrowth ◽  
Casting Process ◽  
Tissue Response ◽  
Free Form ◽  
Bone Area ◽  
Colloidal Processing ◽  
Sintering Condition ◽  
Rabbit Femur

Colloidal processing was used to cast zirconia and hydroxyapatite materials. The cast materials reached densities around 99% when sintered at 1500°C and 1200°C respectively. By controlling the colloidal process the sintered density of hydroxyapatite was also reduced to around 80% when the same sintering condition was used. The casting process was combined with free form fabrication to prepare designed scaffolds with identical macroporosity. These scaffolds were used to evaluate the early bone tissue response in rabbit femur. After six weeks of implantation the bone area in scaffolds of zirconia and hydroxyapatite were compared. In scaffolds of hydroxyapatite the bone area was roughly three times larger compared to corresponding scaffolds of zirconia. When the scaffolds of hydroxyapatite also contained an open microporosity of around 20% the amount of bone was even more pronounced. The results showed the importance of the material composition and the microstructure on the bone regenerating performance of scaffolds.

Microstructure Evolution and Mechanical Properties of the Ni/Ni Soldered Joints

2011 ◽  
Vol 172-174 ◽  
pp. 863-868
Author(s):  
Anna Sypień ◽  
Andrzej Piątkowski ◽  
Paweł Zięba
Keyword(s):  
Mechanical Properties ◽  
Shear Strength ◽  
Chemical Composition ◽  
Microstructure Evolution ◽  
Fracture Behavior ◽  
Bulk Solder ◽  
Subsequent Aging ◽  
Brittle Phase ◽  
Edx Microanalysis ◽  
Solder Interface

The paper presents the results of studies on the microstructure, chemical composition and mechanical properties of the Ni/SnAuCu/Ni interconnections obtained due to the conventional soldering at 300 °C for different times and subsequent aging at 150 °C. The EDX microanalysis allowed to detect at the Ni/solder interface the (Ni,Cu,Au)3Sn4phase which transformed to (Cu,Ni,Au)6Sn5after longer time of soldering. In the central part of the interconnection AuSn4brittle phase was present. This phase was responsible for the significant decrease of the shear strength in the joints subjected to aging at 150 °C for 1000h, 1500 hours. The fracture behavior of such joints appeared to be caused partly by the coalescence of the microvoids in the bulk solder, cleavage of η-phase grains and decohesion at the interface.

scholarly journals Changes with age (from 0 to 37 D) in tibiae bone mineralization, chemical composition and structural organization in broiler chickens

2019 ◽  
Vol 98 (11) ◽  
pp. 5215-5225 ◽  
Author(s):  
Estefania Sanchez-Rodriguez ◽  
Cristina Benavides-Reyes ◽  
Cibele Torres ◽  
Nazaret Dominguez-Gasca ◽  
Ana I Garcia-Ruiz ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Broiler Chickens ◽  
Structural Organization ◽  
Bone Mineralization

scholarly journals Biomechanical behaviours of the bone–implant interface: a review

2019 ◽  
Vol 16 (156) ◽  
pp. 20190259 ◽  
Author(s):  
Xing Gao ◽  
Manon Fraulob ◽  
Guillaume Haïat
Keyword(s):  
Bone Tissue ◽  
Medical Devices ◽  
State Of The Art ◽  
Biomechanical Properties ◽  
Review Article ◽  
Implant Stability ◽  
Bone Implant ◽  
Current State ◽  
Mechanical Methods

In recent decades, cementless implants have been widely used in clinical practice to replace missing organs, to replace damaged or missing bone tissue or to restore joint functionality. However, there remain risks of failure which may have dramatic consequences. The success of an implant depends on its stability, which is determined by the biomechanical properties of the bone–implant interface (BII). The aim of this review article is to provide more insight on the current state of the art concerning the evolution of the biomechanical properties of the BII as a function of the implant's environment. The main characteristics of the BII and the determinants of implant stability are first introduced. Then, the different mechanical methods that have been employed to derive the macroscopic properties of the BII will be described. The experimental multi-modality approaches used to determine the microscopic biomechanical properties of periprosthetic newly formed bone tissue are also reviewed. Eventually, the influence of the implant's properties, in terms of both surface properties and biomaterials, is investigated. A better understanding of the phenomena occurring at the BII will lead to (i) medical devices that help surgeons to determine an implant's stability and (ii) an improvement in the quality of implants.

A New Framework for the Accurate FE Model of Bone Tissue Based on CT Images

2010 ◽  
Vol 44-47 ◽  
pp. 1612-1616
Author(s):  
Xiao Hui Huang ◽  
Guo Qun Zhao ◽  
Wen Guang Liu ◽  
Pei Lai Liu
Keyword(s):  
Bone Tissue ◽  
Material Property ◽  
Geometric Model ◽  
Automatic Segmentation ◽  
Region Of Interest ◽  
Ct Images ◽  
Fe Model ◽  
Volume Of Interest ◽  
Smooth Operator ◽  
New Framework

The frameworks for finite element (FE) model of bone tissue available in pervious literatures, to some extent, are expert-oriented and give rise to a considerable deviation in geometric model and assignment of material property. The objective of this study is to develop a new framework to reconstruct accurate individual bone FE model based on CT images rapidly and conveniently. In image-processing, automatic segmentation of the region of interest (ROIs) improves the efficiency. The idea of enclosed volume of interest (VOI) overcomes the drawback of geometric ambiguity in Marching Cube (MC) method. Geometric model is easily obtained by a STL translator and smooth operator in home-made program. In the material property assignment, two templates for hexahedron and tetrahedron FE models, respectively, are put forth to smoothing an abrupt change of material property in the region from cortical to cancellous. K-mean algorithm is introduced to cluster material properties to improve partition performance. Finally, the new framework is demonstrated by the implementation of a femoral FE model.

scholarly journals Influence of high insertion torque on implant placement: an anisotropic bone stress analysis

2010 ◽  
Vol 21 (6) ◽  
pp. 508-514 ◽  
Author(s):  
Bruno Salles Sotto-Maior ◽  
Eduardo Passos Rocha ◽  
Erika Oliveira de Almeida ◽  
Amilcar Chagas Freitas-Júnior ◽  
Rodolfo Bruniera Anchieta ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Cancellous Bone ◽  
Maximum Principal Stress ◽  
Insertion Torque ◽  
Stress Concentrations ◽  
Bone Implant ◽  
Significance Level ◽  
Bone Stress ◽  
Implant Placement ◽  
Solid Works

The aim of this study was to evaluate the influence of the high values of insertion torques on the stress and strain distribution in cortical and cancellous bones. Based on tomography imaging, a representative mathematical model of a partial maxilla was built using Mimics 11.11 and Solid Works 2010 softwares. Six models were built and each of them received an implant with one of the following insertion torques: 30, 40, 50, 60, 70 or 80 Ncm on the external hexagon. The cortical and cancellous bones were considered anisotropic. The bone/implant interface was considered perfectly bonded. The numerical analysis was carried out using Ansys Workbench 10.0. The convergence of analysis (6%) drove the mesh refinement. Maximum principal stress (σmax) and maximum principal strain (εmax) were obtained for cortical and cancellous bones around to implant. Pearson's correlation test was used to determine the correlation between insertion torque and stress concentration in the periimplant bone tissue, considering the significance level at 5%. The increase in the insertion torque generated an increase in the σmax and εmax values for cortical and cancellous bone. The σmax was smaller for the cancellous bone, with greater stress variation among the insertion torques. The εmax was higher in the cancellous bone in comparison to the cortical bone. According to the methodology used and the limits of this study, it can be concluded that higher insertion torques increased tensile and compressive stress concentrations in the periimplant bone tissue.

scholarly journals Impact of dental implant insertion method on the peri-implant bone tissue: Experimental study

2013 ◽  
Vol 70 (9) ◽  
pp. 807-816
Author(s):  
Novak Stamatovic ◽  
Smiljana Matic ◽  
Zoran Tatic ◽  
Aleksandra Petkovic-Curcin ◽  
Danilo Vojvodic ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Collagen Fibers ◽  
Histopathological Analysis ◽  
Moderate Increase ◽  
Tissue Necrosis ◽  
Two Stage ◽  
Bone Implant ◽  
Osteoblastic Activity ◽  
One Stage ◽  
Collagen Tissue

Background/Aim. The function of dental implants depends on their stability in bone tissue over extended period of time, i.e. on osseointegration. The process through which osseointegration is achieved depends on several factors, surgical insertion method being one of them. The aim of this study was to histopathologically compare the impact of the surgical method of implant insertion on the peri-implant bone tissue. Methods. The experiment was performed on 9 dogs. Eight weeks following the extraction of lower premolars implants were inserted using the one-stage method on the right mandibular side and two-stage method on the left side. Three months after implantation the animals were sacrificed. Three distinct regions of bone tissue were histopathologically analyzed, the results were scored and compared. Results. In the specimens of one-stage implants increased amount of collagen fibers was found in 5 specimens where tissue necrosis was also observed. Only moderate osteoblastic activity was found in 3 sections. The analysis of bone-to-implant contact region revealed statistically significantly better results regarding the amount of collagen tissue fibers for the implants inserted in the two-stage method (Wa = 59 < 66,5, ? = 0.05), but necrosis was found in all specimens, and no osteoblastic activity. Histopathological analysis of bone-implant interface of one-stage implants revealed increased amount of collagen fibers in all specimens, moderate osteoblastic activity and neovascularization in 2 specimens. No inflammation was observed. The analysis of two-stage implants revealed a marked increase of collagen fibers in 5 specimens, inflammation and bone necrosis were found in only one specimen. There were no statistically significant differences between the two methods regarding bone-implant interface region. Histopathological analysis of bone tissue adjacent to the one-stage implant revealed moderate increase of collagen tissue in only 1 specimen, moderate increase of osteoblasts and osteocytes in 3 specimens. No necrotic tissue was found. The analyzed specimens of bone adjacent to two-stage implants revealed a moderate increase in the number of osteocytes in 3 and a marked increase in 6 specimens respectively. This difference was statistically significant (Wb = 106.5 > 105, ? = 0.05). No necrosis and osteoblastic activity were observed. Conclusion. Better results were achieved by the two-stage method in bone-to-implant contact region regarding the amount of collagen tissue, while the results were identical regarding the osteoblastic activity and bone tissue necrosis. There was no difference between the methods in the bone-implant interface region. In the bone tissue adjacent to the implant the results were identical regarding the amount of collagen tissue, osteoblastic reaction and bone tissue necrosis, while better results were achieved by the two-stage method regarding the number of osteocytes.

scholarly journals Osteodensitometric Characteristics of Bone Tissue in Women with Normal Body Weight

2021 ◽  
Vol 10 (3) ◽  
pp. 108-111
Author(s):  
I. G. Pashkova
Keyword(s):  
Body Weight ◽  
Bone Tissue ◽  
Muscle Mass ◽  
Bone Mineralization ◽  
Lumbar Vertebrae ◽  
The Body ◽  
Mineral Density ◽  
Normal Body ◽  
Age Related ◽  
Normal Body Weight

The aim of the study was to investigate age-related changes in bone mineralization indicators in the lumbar vertebrae in women with normal body weight living in the conditions of the Northern region.Material and methods. A complex somatometric examination and quantitative assessment of the bone tissue mineral density in the lumbar vertebrae were performed according to dual-energy X-ray absorptiometry of a group of Slavic women (n=127) with a normal body weight (BMI values from 18.5 to 24.9 kg/m2) aged 20 to 87 years, permanently residing in the Republic of Karelia. Statistical processing of the material was performed using the program "STATISTICA 6.0".Results. The BMI values in women increased significantly every decade of life. Direct correlations of mineral bone density (MBD) with the body length (r=0.46, p<0.001), with the body surface area (r=0.46, p<0.001), with absolute muscle mass (MM) (r=0.39, p<0.001), and with body mass (r=0.29, p<0.001) were revealed. No significant correlation with the adipose mass was found. The incidence of low MBD of the lumbar vertebrae was 48%: osteopenia was in 29%, osteoporosis was in 19% of women. The analysis of the component composition of the body in women with different levels of bone mass showed significant differences in the absolute content of muscle mass.Conclusion. In women with a normal BMI, body weight and muscle mass play an essential role in maintaining lumbar vertebrae bone mineral density.

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