scholarly journals Alveolar Socket Preservation with Different Autologous Graft Materials: Preliminary Results of a Multicenter Pilot Study in Human

Materials ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1153 ◽  
Author(s):  
Elio Minetti ◽  
Edoardo Giacometti ◽  
Ugo Gambardella ◽  
Marcello Contessi ◽  
Andrea Ballini ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Membrane ◽  
Graft Material ◽  
Endodontically Treated Teeth ◽  
Socket Preservation ◽  
Autologous Graft ◽  
Bone Biopsies ◽  
Group 2 ◽  
Autologous Grafts ◽  
Group 1

Background: The histological and histomorphometrical results were evaluated between vital whole and non-vital endodontically treated teeth used as autologous grafts in post-extractive socket preservation procedures. Methods: Twenty-eight patients (average age 51.79 ± 5.97 years) with post-extractive defects were enrolled in five dentistry centers. All patients were divided into two groups: with whole teeth (Group 1) and teeth with endodontical root canal therapy (Group 2). The extracted teeth were processed with the Tooth Transformer device to obtain a demineralized and granulated graft material used with a resorbable collagen membrane for socket preservation. After four months, 32 bone biopsies were obtained for histological, histomorphometric, and statistical analysis. Results: During the bone healing period, no infection signs were observed. Nineteen biopsies in group 1 and 13 biopsies in group 2 were detected. The histological analysis showed neither inflammatory nor infective reaction in both groups. Autologous grafts surrounded by new bone were observed in all samples and, at high magnification, partially resorbed dentin and enamel structures were detected. No gutta-percha or cement was identified. Small non-statistically significant differences between the groups, in total bone volume (BV), autologous graft residual, and vital bone percentage were detected. Conclusions: The study showed that the TT Transformer grafts were capable of producing new vital bone in socket preservation procedures. The histomorphometric results showed no statistical differences comparing whole and endodontically treated teeth in bone regeneration. Further studies will be carried out in order to understand the advantages of the autologous graft materials obtained from the tooth compared with the current biomaterials in bone regeneration treatments.

scholarly journals The Influence of Different Guided Bone Regeneration Procedures on the Contour of Bone Graft after Wound Closure: A Retrospective Cohort Study

Materials ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 583
Author(s):  
Maoxia Wang ◽  
Xiaoqing Zhang ◽  
Yazhen Li ◽  
Anchun Mo
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Substitute ◽  
Wound Closure ◽  
Guided Bone Regeneration ◽  
Collagen Membrane ◽  
Group 4 ◽  
Surgical Template ◽  
Group 2 ◽  
Group 1

The aim of this study was to evaluate the impact of different guided bone regeneration (GBR) procedures on bone graft contour after wound closure in lateral ridge augmentation. A total of 48 patients with 63 augmented sites were included in this study. Participants were divided into 4 groups (n = 12 in each group) based on different surgical procedures: group 1: particulate bone substitute + collagen membrane; group 2: particulate bone substitute + collagen membrane + healing cap, group 3: particulate bone substitute + injectable platelet-rich fibrin (i-PRF) + collagen membrane; group 4: particulate bone substitute + i-PRF + surgical template + collagen membrane. After wound closure, the thickness of labial graft was measured at 0–5 mm apical to the implant shoulder (T0–T5). At T0–T2, the thickness of labial graft in group 4 was significantly higher than the other three groups (p < 0.05). And group 4 showed significantly more labial graft thickness than group 1 and group 2 at T3–T5 (p < 0.05). Within the limitations of this study, the use of i-PRF in combination with the surgical template in GBR may contribute to achieving an appropriate bone graft contour after wound closure.

scholarly journals Effectiveness of Silicon Platelet-Rich Fibrin and Autologous Bone on Bone Regeneration in Rabbit Calvarian Defects: A Radiological and Histological Study

2021 ◽  
Vol 11 (9) ◽  
pp. 4074
Author(s):  
Argimiro Hernández-Suarez ◽  
María Rizo-Gorrita ◽  
Dubraska Suárez-Vega ◽  
Gladys Velazco ◽  
Ivan Rodriguez Gelfenstein ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Oral Surgery ◽  
Histological Study ◽  
Study Groups ◽  
Control Group ◽  
Graft Material ◽  
Platelet Rich Fibrin ◽  
Computed Microtomography ◽  
Autologous Bone ◽  
Group 2

Repairing bone defects in oral surgery often requires the use of bone regeneration techniques. Silicon is an element that has been employed as regeneration material in several studies. In our study, silicon was combined with autologous bone and platelet-rich fibrin (PRF) membranes to analyse the behaviour of this element in bone regeneration. Four circumferential defects were created in the cranial vault of five New Zealand rabbits. The following elements were applied to the regeneration of the defects: (P): PRF; (S): silicon and (B): autologous bone, with the following distribution of study groups: Group 1 (PSB); Group 2 (PS); Group 3 (SB) and Group 4 (CONTROL): unregenerate group. The animals were sacrificed after 3 weeks. Computed microtomography studies (μ-CT) were carried out, as well as histomorphometric ones. The ANOVA statistical test was used with a Bonferroni post-hoc test to compare the results (p ≤ 0.05). Radiologically, groups PSB and SB were better as far as quantity and percentage of healthy bone observed, but not significantly compared to the control group. The PS group was significantly worse. The histological test revealed that the PSB group was the one to present the largest area, percentage and perimeter of mineralised bone. On evaluating the forming bone (osteoid), no difference was observed across the groups with the exception of the bone perimeter, where the SB group was significantly better. The bone height variable showed no significant differences. In conclusion we can state that the combination of PRF, autologous bone and silicon provides good results at 3 weeks whilst the PS group shows the worst results. This highlights the importance of autologous bone forming part of the graft material in order for the bone to mineralise.

Effect of Endocrowns on Fracture Strength and Microleakage of Endodontically Treated Primary Molar Teeth

2019 ◽  
Vol 10 (2) ◽  
pp. 113-119
Author(s):  
Tarek Seddik ◽  
Sera Derelioglu
Keyword(s):  
Fracture Strength ◽  
Compressive Loading ◽  
Class Ii ◽  
Control Group ◽  
Second Primary ◽  
Endodontically Treated Teeth ◽  
Primary Molar ◽  
Molar Teeth ◽  
Group 2 ◽  
Group 1

Objectives: Optimal restoration of endodontically treated teeth is very important for the durability of the endodontic treatment. The focus of this study was to evaluate the fracture strength and microleakage of composite endocrowns compared with Class II composite restorations in endodontically treated primary molar teeth. Materials and Methods: 48 extracted second primary molars were divided into 2 groups. Group 1 (control): teeth with Class II and endodontic access cavities restored with G-aenial composite; group 2: endocrown restorations with G-aenial composite. After completing restorative procedures, teeth were subjected to thermal cycling (500 cycles). Compressive loading was applied to half of the samples, although the other half were immersed in 0.5% basic fucsin solution for 24 h, sectioned, and examined for dye penetration under stereomicroscope. Data were subjected to statistical analysis by the Mann-Whitney U test (α = 0.05). Results: The fracture strength of endocrowns (1741 ± 379.35 N) was significantly higher than that of the control group (1126.5 ± 405.39 N) ( P < .05). No statistical difference was found in microleakage between the 2 groups ( P > .05). Conclusion: Endocrown preparation increases the fracture strength of the final composite restoration when used in primary molar teeth. Composite endocrown restorations can be a practical and an esthetic option for restoring endodontically treated primary molar teeth.

scholarly journals Effects of Platelet-Rich Fibrin on Hard Tissue Healing: A Histomorphometric Crossover Trial in Sheep

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1695
Author(s):  
Esra Ondur ◽  
Nilufer Bolukbasi Balcioglu ◽  
Merva Soluk Tekkesin ◽  
Ozlem Guzel ◽  
Selim Ersanli
Keyword(s):  
Bone Regeneration ◽  
Bone Defects ◽  
Collagen Membrane ◽  
Bovine Bone ◽  
Graft Material ◽  
Hard Tissue ◽  
Platelet Rich Fibrin ◽  
Tissue Healing ◽  
Significant Difference ◽  
Time Point

Bone defects lead to aesthetic and functional losses, causing dental rehabilitation to be more difficult. The objective of this work is to histologically assess the hard tissue healing of bone defects filled with platelet-rich fibrin (PRF) alone or as an adjuvant for mixing with and covering anorganic bovine bone (ABB), compared to ABB covered with a resorbable collagen membrane (CM). This study was designed as a crossover animal study. Four 5-mm tibia defects, 5 mm apart from each other, were surgically created on the tibias of 6 sheep. The defects were randomly filled with ABB + CM; PRF alone; ABB+PRF; or were left empty. The animals were euthanized on days 10, 20, and 40 post-operatively. No group showed any signs of bone necrosis. Inflammation was observed in 2 control and 3 test defects with no statistically significant difference between groups at each time point. The ABB + CM and ABB + PRF groups experienced the highest bone regeneration ratios. No differences between the empty-defect and PRF groups were observed in regard to bone regeneration. No statistical difference was observed between the ABB+PRF and ABB + CM groups in regard to bone regeneration and the amount of residual graft material at each time point. The use of PRF should be preferred due to its autogenous origin, low cost, and ease of use.

scholarly journals Guided Bone Regeneration- A Comprehensive Review

2021 ◽  
Author(s):  
Vineetha Venugopalan ◽  
Anegundi Raghavendra Vamsi ◽  
Santhosh Shenoy ◽  
Karishma Ashok ◽  
Biju Thomas
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Primary Stability ◽  
Guided Bone Regeneration ◽  
Collagen Membrane ◽  
Bony Defect ◽  
Graft Material ◽  
Bone Augmentation ◽  
Wide Range ◽  
Space Maintenance

Successful implant treatment requires prosthetically driven placement of an implant, primary stability at placement, and careful living bone management. The resorptive changes of alveolar bone are an inevitable process following tooth loss, periodontal disease or trauma which causes bone defects. This results in various aesthetic and functional complications such as soft tissue recession, infection and inflammation. Various methods have been tried and advocated for augmenting these bone deficiencies. Guided Bone Regeneration (GBR) is a successful modality for bone augmentation with a wide range of indications and helps restore the alveolar ridge dimensions. It utilises the principle of Guided Tissue Regeneration (GTR) for space maintenance within a bony defect. Different types of barrier membranes are being utilised along with various bone grafts in GBR. Thorough knowledge regarding the biology of bone is required before the initiation of any bone augmentation procedure. A combination of Collagen Membrane (CM) and graft material was found successful for GBR. Hence, this review focuses on presentation of best available evidence for various aspects of GBR.

Accelerated Soft and Hard Tissue Healing In Extraction Sockets Augmented With Bone Allograft and Amnio- Fluid, Clinical and Histological Report of Ten Cases

2019 ◽  
Author(s):  
Mohamed Maksoud
Keyword(s):  
Bone Graft ◽  
Case Series ◽  
Bone Allograft ◽  
Gingival Tissue ◽  
Graft Material ◽  
Extraction Socket ◽  
Socket Preservation ◽  
Bone Graft Material ◽  
Keratinized Tissue ◽  
Bone Biopsies

Preservation of the dental extraction sockets through bone grafting has been a key procedure to maintain the volumetric aspect of the bone walls. This has been proven successful in bone preservation for future implant insertion or prosthetic treatment. Several materials of bone allograft, xenografts, synthetics and membranes were successful in socket preservation however the healing duration and the outcome of bone formed differs. Ten patients selected with posterior extraction sockets for the augmentation procedure, the bone graft mass mixed with Amnio-Spark fluid (Salvin Dental Specialties, Charlotte, NC, USA) and the wound secured with sutures and left to heal with no covering membrane. Post-operative visits scheduled at two, four and twelve weeks’ intervals for monitoring of the healing and core biopsies were harvested from the augmented extraction sockets and examined histologically at the twelve’s week. At two weeks, there was considerable formation of tissue covering the bone graft material which continued to progress into keratinized tissue in four weeks. Core bone biopsies taken at twelve weeks demonstrated a pattern of viable bone formation with minor remnants of the bone graft material. This case series will demonstrate the effectiveness of the amnio- fluid in rapid formation of gingival tissue on top of the augmented extraction socket wounds. Additionally, it will histologically confirm expedited bone remodeling into the extraction socket following the augmentation

scholarly journals Stem Cells Regenerative Properties on New Rat Spinal Fusion Model

2015 ◽  
pp. 119-128
Author(s):  
K. KLÍMA ◽  
V. VANĚČEK ◽  
A. KOHOUT ◽  
O. JIROUŠEK ◽  
R. FOLTÁN ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spinal Fusion ◽  
Tumor Formation ◽  
Collagen Membrane ◽  
Bone Biology ◽  
Fusion Model ◽  
New Model ◽  
Spinous Processes ◽  
Group 2 ◽  
Group 1

Stem cells biology is one of the most frequent topic of physiological research of today. Spinal fusion represents common bone biology challenge. It is the indicator of osteoinduction and new bone formation on ectopic model. The purpose of this study was to establish a simple model of spinal fusion based on a rat model including verification of the possible use of titanium microplates with hydroxyapatite scaffold combined with human bone marrow-derived mesenchymal stem cells (MSCs). Spinous processes of two adjacent vertebrae were fixed in 15 Wistar rats. The space between bony vertebral arches and spinous processes was either filled with augmentation material only and covered with a resorbable collagen membrane (Group 1), or filled with augmentation material loaded with 5 × 106 MSCs and covered with a resorbable collagen membrane (Group 2). The rats were sacrificed 8 weeks after the surgery. Histology, histomorphometry and micro-CT were performed. The new model of interspinous fusion was safe, easy, inexpensive, with zero mortality. We did not detect any substantial pathological changes or tumor formation after graft implantation. We observed a nonsignificant effect on the formation of new bone tissue between Group 1 and Group 2. In the group with MSCs (Group 2) we described minor inflamatory response which indicates the imunomodulational and antiinflamatory role of MSCs. In conclusion, this new model proved to be easy to use in small animals like rats.

Guiding Bone Regeneration with a Novel Biodegradable Polymeric Membrane and Bioceramic Bone Grafts around Dental Implants

2007 ◽  
Vol 330-332 ◽  
pp. 1417-1420
Author(s):  
L. Li ◽  
C.Y. Bao ◽  
Guo Min Ou ◽  
W.C. Chen ◽  
X.J. Zhang ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Dental Implants ◽  
Polymeric Membrane ◽  
Collagen Membrane ◽  
Graft Material ◽  
The Novel ◽  
Histomorphometric Analysis ◽  
Mongrel Dog ◽  
Collagen Membranes

The aim of this study is to evaluate treatment effects of novel GBR membranes (PDLLA membranes) applied to bony defects around dental implants on new bone regeneration alone or in combination with bioceramic bone graft (BIO-OSS®) on the mongrel dog model in comparison with collagen membrane (BIO-GIDE®) and therefore to assess the clinical values of the novel GBR membranes and bioceramic bone graft material in dental implantology. 6 implants were placed in bilateral mandibular of each mongrel dog after preparation of mid- crestal defects. The defects on one side were covered with PDLLA membranes alone or with bone graft, while the contra-lateral sides received collagen membranes alone or with bone graft. These animals were sacrificed at 2, 4, 8,12 weeks post the GBR-operation. And gross sample examination, standardized radiographs, 99mTc-MDP SPECT and histomorphometric analysis were taken. All examinations showed similar amounts of newly formed bone beneath both types of barriers. The PDLLA or collagen membrane with bone graft groups showed better results than the membrane used alone groups. The results indicated that PDLLA membranes especially with bioceramic bone graft presented an improved response in dealing with bony defects around implants.

scholarly journals Influence of Removing or Leaving the Prosthesis after Regenerative Surgery in Peri-Implant Defects: Retrospective Study: 32 Clinical Cases with 2 to 8 Years of Follow-Up

2021 ◽  
Vol 18 (2) ◽  
pp. 645
Author(s):  
Víctor Astolfi ◽  
Alberto Gómez-Menchero ◽  
José Vicente Ríos-Santos ◽  
Pedro Bullón ◽  
Francisco Galeote ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Response To Treatment ◽  
Collagen Membrane ◽  
Chi Square ◽  
Implant Surface ◽  
Reliable Technique ◽  
Healing Period ◽  
Group 2 ◽  
Bone Gain ◽  
Group 1

Purpose: The aim of this retrospective study was to compare the influence of removing or not removing a prosthesis after regenerative surgery on peri-implant defects. Methods: Two different groups were compared (Group 1: removing the prosthesis; Group 2: maintaining the prosthesis), analyzing radiographic bone filling (n = 32 implants) after regenerative treatment in periapical radiographs. The peri-implant defects were measured before and after regenerative treatment using Bio-Oss® (Geistlich Pharma, Wohhusen, Switzerland) and a reabsorbable collagen membrane (Jason®, Botis, Berlin, Germany), the healing period was two years after peri-implant regenerative surgery. Statistical analysis was performed, and a Chi square test was carried out. To determine the groups that made the difference, corrected standardized Haberman residuals were used, and previously a normality test had been applied; therefore, an ANOVA or Mann–Whitney U test was used for the crossover with the non-normal variables in Group 1 and Group 2. Results: The results obtained suggest that a regenerative procedure with xenograft, resorbable membrane, and detoxifying the implant surface with hydrogen peroxide form a reliable technique to achieve medium-term results, obtaining an average bone gain at a radiographic level of 2.84 mm (±1.78 mm) in patients whose prosthesis was not removed after peri-implant bone regenerative therapy and 2.18 mm (±1.41 mm) in patients whose prosthesis was removed during the healing period. Conclusions: There are no statistically significant differences in the response to treatment when removing or keeping the prosthesis after regenerative surgery in peri-implant defects.

scholarly journals Use of a Partial-thickness Flap for Guided Bone Regeneration in the Upper Jaw

2017 ◽  
Vol 18 (12) ◽  
pp. 1117-1121 ◽  
Author(s):  
Alberto Busa ◽  
Gianluigi Caccianiga
Keyword(s):  
Bone Regeneration ◽  
Surgical Techniques ◽  
Guided Bone Regeneration ◽  
Vascular Supply ◽  
Healing Time ◽  
Collagen Membrane ◽  
Graft Material ◽  
Implant Placement ◽  
Partial Thickness ◽  
Upper Jaw

ABSTRACT Introduction Guided bone regeneration (GBR) aims to restore adequate bone volume for the placement of implants in an ideal location. In this article, we analyze different surgical techniques for tissue management during GBR and a modified partialthickness surgical approach. Materials and methods In a year's time span, five patients were enrolled in the study: Four women and one man (aged 44–59 years). In four patients, a GBR with simultaneous implant placement was adopted, whereas in another patient, a delayed implant placement was done. The flap was of full thickness and overturned on the side palatal with the aim of a retractor, thus exposing the bone crest. The graft material was covered and protected with a resorbable collagen membrane (Geistlich Bio- Gide®, Switzerland). The periosteal layer of the flap was then positioned above the resorbable membrane without traction. The sutures as vertical mattress were then positioned. Each patient received an intramuscular betamethasone dose (4 mg/50 kg) and antibiotic therapy for 7 days (amoxicillin + clavulanic acid 1 gm every 12 hours) and was instructed to maintain oral hygiene and appropriate wound cleaning. The patients were recalled at different times to monitor the healing. Results No cases of tissue dehiscence were observed during the period of wound healing. One patient, however, showed a delayed exposure 4 months after surgery. This occurrence was managed without complications for the patient. Conclusion The design of proposed flap seems to be effective in controlling the risk of dehiscence during the healing time in the GBR. The vascular supply was rarely compromised. The results we obtained are encouraging even if further studies on this technique are needed. Clinical significance Lateral partial-thickness flaps seem to be effective in controlling tissue tension and consequently the risk of dehiscence. The incision vestibularly performed should favor the soft tissue healing. How to cite this article Porcaro G, Busa A, Bianco E, Caccianiga G, Maddalone M. Use of a Partial-thickness Flap for Guided Bone Regeneration in the Upper Jaw. J Contemp Dent Pract 2017;18(12):1117-1121.

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