scholarly journals Neutralized Dicalcium Phosphate and Hydroxyapatite Biphasic Bioceramics Promote Bone Regeneration in Critical Peri-Implant Bone Defects

Materials ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 823 ◽  
Author(s):  
Hao-Hueng Chang ◽  
Chun-Liang Yeh ◽  
Yin-Lin Wang ◽  
Kang-Kuei Fu ◽  
Shang-Jye Tsai ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Dicalcium Phosphate ◽  
Control Group ◽  
New Bone Formation ◽  
Control Groups ◽  
Fast Bone ◽  
And Control ◽  
Bone Coverage

The aim of this study was to evaluate the efficacy of bone regeneration in developed bioceramics composed of dicalcium phosphate and hydroxyapatite (DCP/HA). Critical bony defects were prepared in mandibles of beagles. Defects were grafted using DCP/HA or collagen-enhanced particulate biphasic calcium phosphate (TCP/HA/Col), in addition to a control group without grafting. To assess the efficacy of new bone formation, implant stability quotient (ISQ) values, serial bone labeling, and radiographic and histological percentage of marginal bone coverage (PMBC) were carefully evaluated four, eight, and 12 weeks after surgery. Statistically significant differences among the groups were observed in the histological PMBC after four weeks. The DCP/HA group consistently exhibited significantly higher ISQ values and radiographic and histological PMCB eight and 12 weeks after surgery. At 12 weeks, the histological PMBC of DCP/HA (72.25% ± 2.99%) was higher than that in the TCP/HA/Col (62.61% ± 1.52%) and control groups (30.64% ± 2.57%). After rigorously evaluating the healing of biphasic DCP/HA bioceramics with a critical size peri-implant model with serial bone labeling, we confirmed that neutralized bioceramics exhibiting optimal compression strength and biphasic properties show promising efficacy in fast bone formation and high marginal bone coverage in peri-implant bone defects.

scholarly journals Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model

2018 ◽  
Author(s):  
Fereydoon Sargolzaei Aval ◽  
Eshaghali Saberi ◽  
Mohammad Reza Arab ◽  
Narjes Sargolzaei ◽  
Esmaeel Zare ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Matrix ◽  
Octacalcium Phosphate ◽  
Bone Defects ◽  
Osteogenic Potential ◽  
Control Group ◽  
New Bone Formation ◽  
Mandibular Bone

Objective: Regeneration of bone defects remains a challenge for maxillofacial and reparative surgeons. The purpose of this histological study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination in artificially created mandibular bone defects in rats. The quality of the newly formed bone was also evaluated. Methods: Thirty-six male Sprague Dawley rats (6-8 weeks old with 120-150 g weight) were randomly divided into four groups. Defects (3 mm in diameter and 2 mm in depth) were created in the mandible of rats and filled with 6 mg of OCP, BMG or a combination of both (1/4 ratio), respectively. Defects were left unfilled in the control group. To assess osteoinduction and bone regeneration and determine the quality of the newly formed bone, tissue specimens were harvested at seven, 14, and 21 days post-implantation. The specimens were processed, stained with hematoxylin and eosin (H&E) and histologically analyzed under light microscopy. Results: In the experimental groups, new bone formation was initiated at the margins of defects from seventh day after implantation. At the end of the study period, the amount of the newly formed bone increased and the bone was relatively mature. Osteoinduction and new bone formation were greater in OCP/BMG group. In the control group, slight amount of new bone had been formed at the defect margins (next to host bone) on day 21. Conclusion: Combination of OCP/BMG may serve as an optimal biomaterial for treatment of mandibular bone defects.

scholarly journals Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

Materials ◽  
2019 ◽  
Vol 12 (10) ◽  
pp. 1613
Author(s):  
Eunhye Jang ◽  
Ja-Youn Lee ◽  
Eun-Young Lee ◽  
Hyun Seok
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Human Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Morphogenetic Protein ◽  
Regeneration Effect ◽  
Recombinant Human Bone

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.

scholarly journals BAG S53P4 putty as bone graft substitute – a rabbit model

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Ilkka Saarenpää ◽  
Patricia Stoor ◽  
Janek Frantzén
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Proximal Tibia ◽  
Rabbit Model ◽  
Bone Graft Substitute ◽  
Control Group ◽  
The Novel ◽  
Bone Augmentation ◽  
New Bone Formation ◽  
New Zealand White Rabbits

AbstractBioactive glass (BAG) S53P4 granules represent a bone augmentation biomaterial for the surgical treatment of bony defects, even in challenging conditions such as osteomyelitis. The aim of this eight-week rabbit implantation study was to evaluate the biocompatibility and bone regeneration performance of a BAG S53P4 putty formulation following its implantation into the proximal tibia bone of twenty-eight New Zealand white rabbits. BAG S53P4 putty was compared to BAG S53P4 granules (0.5-0.8 mm) to evaluate whether the synthetic putty binder influences the bone regeneration of the osteostimulative granules. The putty formulation facilitates clinical use because of its mouldability, injectability and ease of mixing with autograft. Implantation of putty and granules into proximal tibia defects resulted in good osseointegration of the two groups. Both biomaterials were biocompatible, showed high new bone formation, high vascularization and periosteal growth. No signs of disturbed bone formation were observed due to the PEG-glycerol binder in the BAG S53P4 putty. Instead, intramedullary ossification and stromal cell reaction were more advanced in the putty group compared to the control group (p = 0.001 and p < 0.001). In conclusion, the novel mouldable BAG S53P4 putty showed reliable bone regeneration in bony defects without adverse tissue or cell reactions.

Effects of Platelet-Rich Plasma and Chitosan Combination on Bone Regeneration in Experimental Rabbit Cranial Defects

2010 ◽  
Vol 36 (3) ◽  
pp. 175-184 ◽  
Author(s):  
ErolÖzgür Oktay ◽  
Bahtiyar Demiralp ◽  
Burak Demiralp ◽  
Sevda Senel ◽  
Abdullah Cevdet Akman ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Platelet Rich Plasma ◽  
The Other ◽  
New Bone Formation ◽  
Control Groups ◽  
Demarcation Line ◽  
Beneficial Effects ◽  
Derivatives Of ◽  
Prp Gel

Abstract This study aimed to investigate and compare the effect of chitosan sponge and platelet-rich plasma (PRP) gel alone as well as their combination on bone regeneration in rabbit cranial defects. Four cranial defects with a 4.5-mm diameter were created in rabbit cranium and grafted with PRP, chitosan sponge alone, and chitosan sponge incorporated with PRP. The rabbits were killed by the fourth and eighth weeks, and the defects were analyzed histologically. Higher bone formation was observed in the PRP group when compared with the other groups at weeks 4 and 8. All parts of the defects were filled with thick trabecular new bone in the PRP group. The amount of new bone formation in the control groups was found to be less when compared with the PRP group and the least in the chitosan group. The defects that were filled with chitosan sponge showed a limited amount of new bone formation and an obvious fibrous demarcation line between chitosan particles and bone. Application of PRP showed a histological tendency toward increased bone formation. Other forms or derivatives of chitosan may have beneficial effects to achieve new bone regeneration.

Histomorphology on Healing of the Chitosan Membrane and β-TCP on Dental Implant Dehiscence Defects in Dogs

2006 ◽  
Vol 309-311 ◽  
pp. 255-258
Author(s):  
Y.S. Kim ◽  
Tae Gyun Kim ◽  
Ui Won Jung ◽  
C.S. Kim ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Bone Formation ◽  
Alveolar Bone ◽  
Bone Substitutes ◽  
Bone Defects ◽  
Autogenous Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Chitosan Membrane ◽  
Space Maintenance ◽  
Experimental Group

Dehiscence bone defects, frequently observed on dental implants placed in periodontitis-affected alveolar bone or extraction sockets were treated with β-tricalcium phosphate (β –TCP) and chitosan membrane for guided bone regeneration, and the new bone formation on the treated sites were studied. Beagle dogs were used for the experiment. First to fourth mandibular premolars were extracted, and the post extraction alveolar bone surface was planed. After 8 weeks of healing, 3 by 4mm dehiscence defects were created using straight fissure burs. Total of 16 oxidized titanium surface implants were placed on the bone defects of the subjects, two on each side. Control sites were treated with implants only. Experimental Group 1 sites were treated with implants and chitosan membrane. Experimental Group 2 sites were treated with implants, β-TCP and chitosan membrane. Experimental Group 3 sites were treated with implants, β-TCP, autogenous bone and chitosan membrane. The animals were sacrificed 12 weeks after implant placement, and the specimens from the treated sites were histologically studied with following results. Limited amount of new bone formation was observed in control group with unexposed membrane. Slightly greater amount of bone formation was observed on sites treated with β-TCP+membrane or autogenous bone+ β-TCP+membrane compared to control group. Remnants of chitosan membrane and β-TCP encapsulated with connective tissue were observed during experimental periods. These results suggest that further studies are needed on membrane rigidity and infection control for space maintenance underneath the membrane and bone substitutes in the treatment of dehiscence defects.

scholarly journals The Use of a Synthetic Ceramic Graft Mixed with Platelet-Rich Fibrin (PRF) in the Treatment of Bone Defects.An experimental study

2019 ◽  
Vol 56 (4) ◽  
pp. 774-778
Author(s):  
Alexandru Nemtoi ◽  
Ana Nemtoi ◽  
Kamel Earar ◽  
Adrian Beznea ◽  
Cristian Onisor ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Wistar Rats ◽  
Bone Defects ◽  
Histologic Examination ◽  
New Bone Formation ◽  
Platelet Rich Fibrin

The aim of this study is to investigate the effect of alloplatic graft and platelet rich fibrin (PRF) , alone or in combination, on bone regeneration in Wistar rats femural defects. Bone defects were prepared in adults male of 20 Wistar rats. In a group of ten, the defect was filled with synthetic ceramic graft as an alloplastic graft; the others 10 were grafted with this alloplastic graft mixed with PRF. All animals were sacrificed on the 90th postoperative day and the femural bones were removed, histologic sections were prepared and the experimental sites were examined microscopically. Histologic examination revealed more new bone formation in the defects filled with alloplastic graft mixed with PRF than in the defects grafted with alloplastic graft alone. The platelet rich fibrin combination effectively induces new bone formation.

Effect of Synthetic β-TCP and Bovine-Derived Hydroxyapatite Grafting on Bone Regeneration in Rats

2007 ◽  
Vol 361-363 ◽  
pp. 1261-1264
Author(s):  
C.S. Lim ◽  
S.G. Kim ◽  
Sung Chul Lim
Keyword(s):  
Bone Formation ◽  
Light Microscopy ◽  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Bone Defects ◽  
Frontal Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Grafting Materials ◽  
Defect Margin

We evaluated the bone healing effect of grafting with synthetic β-tricalcium phosphate (β-TCP; Cerasorb®), bovine-derived hydroxyapatite (HA; Bio-Oss®), and a mixture of β-TCP and HA in rats. Each material was grafted in prepared 8-mm frontal bone defects in 15 rats. The control group underwent surgery without any grafting materials and was examined after 4 weeks, whereas the experimental groups received grafting materials and were examined after 1, 2, and 4 weeks. After implantation, the rats were sacrificed for histomorphometric studies using light microscopy, and the data were analyzed using analysis of variance. Considerable inflammation and fibrosis were observed after 1 and 2 weeks in all experimental groups, whereas the inflammation was reduced and fibrosis was stabilized after 4 weeks. New bone formation was observed at the defect margin. Statistically, there was no difference in new bone formation among the three experimental groups. In conclusion, there was no difference in new bone formation using Bio-Oss®, Cerasorb®, and a mixture of Bio-Oss® and Cerasorb®.

Guided Bone Regeneration (GBR) on Healing Bone Defects: A Histological Study in Rabbits

2004 ◽  
Vol 5 (2) ◽  
pp. 114-123 ◽  
Author(s):  
Muzaffer Asian ◽  
Göksel Şimşek ◽  
Ertunҫ Dayi
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Optical Microscopy ◽  
Bone Healing ◽  
Histological Study ◽  
Bone Defects ◽  
Guided Bone Regeneration ◽  
Control Group ◽  
Healing Bone ◽  
The Right

Abstract In this study, the effects of guided bone regeneration (GBR) on the healing of bone defects were evaluated. Resorbable membranes were placed in experimentally formed cavities in the right posterior tibia of 30 rabbits. Decalcified histological sections were evaluated using optical microscopy at 10, 20, and 30 days after GBR. Osteocondrial bone union, active bone formation and spongiosal bone formation values of the GBR group are higher than the control group. It was found that GBR technique had a positive and accelerating influence in all phases of bone healing. Citation Aslan M, Şimşek G, Dayi E. Guided Bone Regeneration (GBR) on Healing Bone Defects: A Histological Study in Rabbits. J Contemp Dent Pract 2004 May;(5)2:114-123.

scholarly journals Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs

2017 ◽  
Vol 47 (7) ◽  
Author(s):  
Endrigo Gabellini Leonel Alves ◽  
Rogéria Serakides ◽  
Isabel Rodrigues Rosado ◽  
Omar Leonardo Aristizabal Paez ◽  
Jéssica Alejandra Castro Varon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Bone Formation ◽  
Bone Defects ◽  
Autogenous Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Osteoprogenitor Cells ◽  
Critical Bone Defects

ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days.

scholarly journals Glenoid Fossa Responses to Mandibular Lateral Shift in Growing Rats

2007 ◽  
Vol 77 (4) ◽  
pp. 660-667 ◽  
Author(s):  
Chang Liu ◽  
Sawa Kaneko ◽  
Kunimichi Soma
Keyword(s):  
Bone Formation ◽  
Contralateral Side ◽  
Lateral Shift ◽  
Posterior Region ◽  
Control Group ◽  
Glenoid Fossa ◽  
New Bone Formation ◽  
Ipsilateral Side ◽  
Growing Rats ◽  
And Control

Abstract Objective: To evaluate the morphological and histological responses of the glenoid fossa to mandibular lateral shift in growing rats. Materials and Methods: A resin plate was placed on the upper incisors of 4-week-old rats in the experimental groups to displace the mandible to the left during closure. The rats were killed after 2, 4, 8, and 12 weeks. The morphometric measurements were performed on dry skulls, and tissue blocks were processed for periodic acid and Schiff's reagent (PAS) staining to examine the new bone formation. Results: Gross measurements showed asymmetry in both the position and size of the fossae between the two sides after 4 weeks of lateral shift. The glenoid fossa on the ipsilateral side was repositioned relatively backward, outward and upward compared with the contralateral side and control group, whereas the fossa on the contralateral side was relocated relatively forward and downward compared with the control group. The length of the fossa was smaller on the ipsilateral side than on contralateral side and control group. At 2 weeks, the amount of newly formed bone in the posterior region of the fossa was higher in the experimental group than the control group. Conclusion: It is suggested that the mandibular lateral shift causes asymmetry in the position and size of the glenoid fossa and that this phenomenon can be related to different bilateral directional new bone formation in the posterior region.

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