scholarly journals The Effect of Stabilisation Agents on the Immunomodulatory Properties of Gold Nanoparticles Obtained by Ultrasonic Spray Pyrolysis

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4121 ◽  
Author(s):  
Marina Bekić ◽  
Sergej Tomić ◽  
Rebeka Rudolf ◽  
Marijana Milanović ◽  
Dragana Vučević ◽  
...  
Keyword(s):  
Immune Response ◽  
Gold Nanoparticles ◽  
Spray Pyrolysis ◽  
Inflammatory Cytokines ◽  
Mononuclear Cells ◽  
Biological Fluids ◽  
Ultrasonic Spray Pyrolysis ◽  
Drug Carriers ◽  
Ultrasonic Spray ◽  
Pro Inflammatory Cytokines

Gold nanoparticles (GNPs) have been investigated extensively as drug carriers in tumour immunotherapy in combination with photothermal therapy. For this purpose, GNPs should be stabilised in biological fluids. The goal of this study was to examine how stabilisation agents influence cytotoxicity and immune response in vitro. Spherical GNPs, 20 nm in size, were prepared by ultrasonic spray pyrolysis (USP). Three types of stabilising agents were used: sodium citrate (SC), polyvinyl-pyrrolidone (PVP), and poly-ethylene glycol (PEG). Pristine, non-stabilised GNPs were used as a control. The culture models were mouse L929 cells, B16F10 melanoma cells and human peripheral blood mononuclear cells (PBMNCs), obtained from healthy donors. Control SC- and PEG-GNPs were non-cytotoxic at concentrations (range 1–100 µg/mL), in contrast to PVP-GNPs, which were cytotoxic at higher concentrations. Control GNPs inhibited the production of IFN-ϒ slightly, and augmented the production of IL-10 by PHA-stimulated PBMNC cultures. PEG-GNPs inhibited the production of pro-inflammatory cytokines (IL-1, IL-6, IL-8, TNF-α) and Th1-related cytokines (IFN-ϒ and IL-12p70), and increased the production of Th2 cytokines (IL-4 and IL-5). SC-PEG inhibited the production of IL-8 and IL-17A. In contrast, PVP-GNPs stimulated the production of pro-inflammatory cytokines, Th1 cytokines, and IL-17A, but also IL-10. When uptake of GNPs by monocytes/macrophages in PBMNC cultures was analysed, the ingestion of PEG- GNPs was significantly lower compared to SC- and PVP-GNPs. In conclusion, stabilisation agents modulate biocompatibility and immune response significantly, so their adequate choice for preparation of GNPs is an important factor when considering the use of GNPs for application in vivo.

Cytotoxicity of Gold Nanoparticles Prepared by Ultrasonic Spray Pyrolysis

2010 ◽  
Vol 26 (5) ◽  
pp. 595-612 ◽  
Author(s):  
R. Rudolf ◽  
B. Friedrich ◽  
S. Stopić ◽  
I. Anžel ◽  
S. Tomić ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Spray Pyrolysis ◽  
Ultrasonic Spray Pyrolysis ◽  
Ultrasonic Spray

Formation mechanisms for gold nanoparticles in a redesigned Ultrasonic Spray Pyrolysis

2017 ◽  
Vol 28 (3) ◽  
pp. 876-883 ◽  
Author(s):  
Peter Majerič ◽  
Darja Jenko ◽  
Bernd Friedrich ◽  
Rebeka Rudolf
Keyword(s):  
Gold Nanoparticles ◽  
Spray Pyrolysis ◽  
Ultrasonic Spray Pyrolysis ◽  
Formation Mechanisms ◽  
Ultrasonic Spray

Application of Gold(III) Acetate as a New Precursor for the Synthesis of Gold Nanoparticles in PEG Through Ultrasonic Spray Pyrolysis

2017 ◽  
Vol 28 (3) ◽  
pp. 1647-1665 ◽  
Author(s):  
Mohammed Shariq ◽  
Peter Majerič ◽  
Bernd Friedrich ◽  
Bojan Budic ◽  
Darja Jenko ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Spray Pyrolysis ◽  
Ultrasonic Spray Pyrolysis ◽  
Ultrasonic Spray

scholarly journals In SituSynthesis and Deposition of Gold Nanoparticles with Different Morphologies on Glass and ITO Substrate by Ultrasonic Spray Pyrolysis

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
María de la Garza ◽  
Israel López ◽  
Idalia Gómez
Keyword(s):  
Gold Nanoparticles ◽  
Spray Pyrolysis ◽  
Indium Tin Oxide ◽  
Average Particle Size ◽  
Ultrasonic Spray Pyrolysis ◽  
Average Particle ◽  
Particle Sizes ◽  
Ultrasonic Spray ◽  
Ito Film

Gold nanoparticles were synthesized and depositedin situby ultrasonic spray pyrolysis on glass and indium tin oxide (ITO) substrates. This technique led to the formation of gold nanoparticles with different morphologies without the use of any capping agent. The gold nanoparticles deposited on glass substrate were obtained as nanospheres with an average particle size of 30 nm with some agglomerates; however, the nanoparticles deposited on ITO substrate were obtained with different morphologies, such as triangular nanoprisms, nanorods, nanocubes, and nanorhombus, with particle sizes between 40 and 100 nm. The ITO substrate influenced the morphology of the gold nanoparticles obtained due to changes in the deposition temperature, which also change the crystalline structure of the ITO film on the substrate.

scholarly journals Successful Synthesis of Gold Nanoparticles through Ultrasonic Spray Pyrolysis from a Gold(III) Nitrate Precursor and Their Interaction with a High Electron Beam

ChemistryOpen ◽  
2018 ◽  
Vol 7 (7) ◽  
pp. 533-542 ◽  
Author(s):  
Mohammed Shariq ◽  
Bernd Friedrich ◽  
Bojan Budic ◽  
Nejc Hodnik ◽  
Francisco Ruiz-Zepeda ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Electron Beam ◽  
Spray Pyrolysis ◽  
Ultrasonic Spray Pyrolysis ◽  
High Electron ◽  
Ultrasonic Spray ◽  
Nitrate Precursor

scholarly journals SYNTHESIS OF GOLD NANOPARTICLES THROUGH ULTRASONIC SPRAY PYROLYSIS AND ITS APPLICATION IN PRINTED ELECTRONICS

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Rebeka Rudolf ◽  
Mohammed Shariq ◽  
Valentina Veselinovic ◽  
Tijana Adamovic ◽  
Rajko Bobovnik ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Spray Pyrolysis ◽  
Process Parameters ◽  
Printed Electronics ◽  
Ultrasonic Spray Pyrolysis ◽  
Precursor Solution ◽  
Second Step ◽  
Synthesis Mechanism ◽  
Ultrasonic Spray ◽  
Edx Analyses

This work presents a review of Ultrasonic Spray Pyrolysis (USP) as a technique for the synthesis of gold nanoparticle (AuNPs). The synthesis mechanism involved the preparation of the precursor solution from Au (III) Acetate and the study of the process parameters. AuNPs were characterized with different techniques, such as DLS, UV-vis and SEM-EDX analyses. In the second step, the AuNPs` ink was prepared with the following procedure: (i) Concentration of the AuNPs through Rotavapor, (ii) Filtration of the concentrated AuNPs through Amicon Ultra 15 Centrifugal Filters. Then, it was used directly for printing on an Ag plate. In the final part, we present some information about AuNPs` future applications, which could be in printed electronics with conducted patterns.

Deposition of gold nanoparticles on glass substrate by ultrasonic spray pyrolysis

2010 ◽  
Vol 174 (1-3) ◽  
pp. 9-12 ◽  
Author(s):  
María de la Garza ◽  
Tomás Hernández ◽  
Rafael Colás ◽  
Idalia Gómez
Keyword(s):  
Gold Nanoparticles ◽  
Spray Pyrolysis ◽  
Glass Substrate ◽  
Ultrasonic Spray Pyrolysis ◽  
Ultrasonic Spray

Fireworks: How to Simulate the Manufacture and Operation in the Atmosphere with the Substitution of Ultrasonic Spray Pyrolysis

2018 ◽  
Vol 15 (2) ◽  
pp. 147-156
Author(s):  
Rebeka Rudolf ◽  
Urban Ferčec ◽  
Mohammed Shariq
Keyword(s):  
Spray Pyrolysis ◽  
Final Section ◽  
Web Of Science ◽  
Raw Materials ◽  
Ultrasonic Spray Pyrolysis ◽  
Physiochemical Properties ◽  
Physical Size ◽  
Respiratory Problems ◽  
Ultrasonic Spray ◽  
Temperature Ranges

Background: This review provides a closer look at recent work in the field of fireworks manufacture, which could see the replacement of micron-sized particles with their nano-scaled counterparts. Moreover, we also discuss micron-sized particles as well as nanoparticles (NPs) from K, Fe, Al, Ti, Ba, etc., that are produced in the atmosphere as a result of these fireworks. One of the possible technological substitutes for fireworks is presented in detail, i.e., the use of ultrasonic spray pyrolysis (USP) technology. Method: We searched Google, Web of Science and PubMed for a literature survey of fireworks and their products: firecrackers, micron-sized and nanoparticles. Moreover, we used some of our own knowledge and experimental data to strengthen the possibility of simulating the synthesis of firework products on the laboratory scale. Results: The use of nano reactants and oxidisers has seen a substantial increase in the sound efficiency and a decrease in the amount of chemicals used, making fireworks more eco-friendly. The application of Al- and Ti-based nano flash powder in the size range from 35 nm to 50 μm resulted in a significant improvement in the ignition properties of the fireworks. Under changing aerodynamic conditions, it is difficult to collect them as samples for real-time monitoring, needed for their characterization or the testing of their harmfulness under laboratory conditions. As a result, NPs below 100 nm in the surroundings could be easily inhaled into the lungs and cause more pulmonary and respiratory problems than micron-sized particles. USP produces nanoparticles in the laboratory that could replace the conventional micron-sized firecracker raw materials, or nanoparticles that are similar to those formed by fireworks. It will also help to identify the physiochemical properties of the airborne particulates in order to understand and evaluate their impact. </P><P> This review could be valuable for a controlled economic synthesis through USP, and in the use of nanopowders in pyrotechnology that could reduce pollution to a great extent, thus contributing to the growth and good practise of the fireworks industry. With respect to the USP synthesis, we have also discussed in detail the physical (size, shape) and chemical (composition) characteristics of Al2O3 and TiO2 NPs from different precursors and their temperature ranges. An in-depth explanation for a comparative analysis for the formation mechanism of nanoparticles through both fireworks and USP is presented in the final section. We can produce nanoparticles in the laboratory with ultrasonic spray pyrolysis that have similar properties to those produced from fireworks and can then be used for further testing.

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