scholarly journals The Potential Selective Cytotoxicity of Poly (L- Lactic Acid)-Based Scaffolds Functionalized with Nanohydroxyapatite and Europium (III) Ions toward Osteosarcoma Cells

Materials ◽  
2019 ◽  
Vol 12 (22) ◽  
pp. 3779 ◽  
Author(s):  
Mateusz Sikora ◽  
Klaudia Marcinkowska ◽  
Krzysztof Marycz ◽  
Rafał Jakub Wiglusz ◽  
Agnieszka Śmieszek
Keyword(s):  
Lactic Acid ◽  
Metabolic Activity ◽  
Surgical Techniques ◽  
Human Adipose Tissue ◽  
Osteosarcoma Cells ◽  
Selective Cytotoxicity ◽  
Anticancer Properties ◽  
Human Osteosarcoma Cells ◽  
Regeneration Of Bone Tissue ◽  
Carrier Analysis

Osteosarcoma (OSA) is malignant bone tumor, occurring in children and adults, characterized by poor prognosis. Despite advances in chemotherapy and surgical techniques, the survival of osteosarcoma patients is not improving significantly. Currently, great efforts are taken to identify novel selective strategies, distinguishing between cancer and normal cells. This includes development of biomimetic scaffolds with anticancer properties that can simultaneously support and modulate proper regeneration of bone tissue. In this study cytotoxicity of scaffolds composed from poly (L-lactic acid) functionalized with nanohydroxyapatite (nHAp) and doped with europium (III) ions—10 wt % 3 mol % Eu3+: nHAp@PLLA was tested using human osteosarcoma cells: U-2 OS, Saos-2 and MG-63. Human adipose tissue-derived stromal cells (HuASCs) were used as non-transformed cells to determine the selective cytotoxicity of the carrier. Analysis included evaluation of cells morphology (confocal/scanning electron microscopy (SEM)), metabolic activity and apoptosis profile in cultures on the scaffolds. Results obtained indicated on high cytotoxicity of scaffolds toward all OSA cell lines, associated with a decrease of cells’ viability, deterioration of metabolic activity and activation of apoptotic factors determined at mRNA and miRNA levels. Simultaneously, the biomaterials did not affect HuASCs’ viability and proliferation rate. Obtained scaffolds showed a bioimaging function, due to functionalization with luminescent europium ions, and thus may find application in theranostics treatment of OSA.

scholarly journals Selective Cytotoxicity against Human Osteosarcoma Cells by a Novel Synthetic C-1 Analogue of 7-Deoxypancratistatin Is Potentiated by Curcumin

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e28780 ◽  
Author(s):  
Dennis Ma ◽  
Phillip Tremblay ◽  
Kevinjeet Mahngar ◽  
Jonathan Collins ◽  
Tomas Hudlicky ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Selective Cytotoxicity ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals CLEFMA Activates the Extrinsic and Intrinsic Apoptotic Processes through JNK1/2 and p38 Pathways in Human Osteosarcoma Cells

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3280 ◽  
Author(s):  
Jia-Sin Yang ◽  
Renn-Chia Lin ◽  
Yi-Hsien Hsieh ◽  
Heng-Hsiung Wu ◽  
Geng-Chung Li ◽  
...  
Keyword(s):  
Annexin V ◽  
Synthetic Analog ◽  
Osteosarcoma Cells ◽  
Apoptotic Pathway ◽  
Term Survival ◽  
Anticancer Properties ◽  
Human Osteosarcoma ◽  
Effector Caspase ◽  
Human Osteosarcoma Cells ◽  
Induced Apoptosis

Due to the poor prognosis of metastatic osteosarcoma, chemotherapy is usually employed in the adjuvant situation to improve the prognosis and the chances of long-term survival. 4-[3,5-Bis(2-chlorobenzylidene)-4-oxo-piperidine-1-yl]-4-oxo-2-butenoic acid (CLEFMA) is a synthetic analog of curcumin and possesses anti-inflammatory and anticancer properties. To further obtain information regarding the apoptotic pathway induced by CLEFMA in osteosarcoma cells, microculture tetrazolium assay, annexin V-FITC/PI apoptosis staining assay, human apoptosis array, and Western blotting were employed. CLEFMA dose-dependently decreased the cell viabilities of human osteosarcoma U2OS and HOS cells and significantly induced apoptosis in human osteosarcoma cells. In addition to the effector caspase 3, CLEFMA significantly activated both extrinsic caspase 8 and intrinsic caspase 9 initiators. Moreover, CLEFMA increased the phosphorylation of extracellular signal-regulated protein kinases (ERK)1/2, c-Jun N-terminal kinases (JNK)1/2 and p38. Using inhibitors of JNK (JNK-in-8) and p38 (SB203580), CLEFMA’s increases of cleaved caspases 3, 8, and 9 could be expectedly suppressed, but they could not be affected by co-treatment with the ERK inhibitor (U0126). Conclusively, CLEFMA activates both extrinsic and intrinsic apoptotic pathways in human osteosarcoma cells through JNK and p38 signaling. These findings contribute to a better understanding of the mechanisms responsible for CLEFMA’s apoptotic effects on human osteosarcoma cells.

Antiproliferative properties of oleuropein in human osteosarcoma cells

2016 ◽  
Author(s):  
Jose M Moran ◽  
Olga Leal-Hernandez ◽  
Maria L Canal-Macias ◽  
Jesus M Lavado-Garcia ◽  
Raul Roncero-Martin ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

Copper(II) Complexes with Saccharinate and Glutamine as Antitumor Agents: Cytoand Genotoxicity in Human Osteosarcoma Cells

2017 ◽  
Vol 17 (3) ◽  
pp. 424-433 ◽  
Author(s):  
Juan Cadavid-Vargas ◽  
Ignacio Leon ◽  
Susana Etcheverry ◽  
Eduardo Santi ◽  
Maria Torre ◽  
...  
Keyword(s):  
Antitumor Agents ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

scholarly journals Assessment of the Nutraceutical Effects of Oleuropein and the Cytotoxic Effects of Adriamycin, When Administered Alone and in Combination, in MG-63 Human Osteosarcoma Cells

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 354
Author(s):  
Katerina Gioti ◽  
Anastasia Papachristodoulou ◽  
Dimitra Benaki ◽  
Nektarios Aligiannis ◽  
Alexios-Leandros Skaltsounis ◽  
...  
Keyword(s):  
Chemotherapeutic Agent ◽  
Molecular Techniques ◽  
Osteosarcoma Cells ◽  
Elisa Method ◽  
Chain Reaction ◽  
Cytotoxic Effects ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Anti Cancer ◽  
Polymerase Chain

Oleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of Olea europaea L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it presents significant side effects. The aim of the present study was to investigate the effect of oleuropein alone (20 μg/mL) and in co-treatment with ADR (50 nM), in MG-63 human osteosarcoma cells. Therefore, cellular and molecular techniques, such as MTT assay, flow cytometry, real-time Polymerase Chain Reaction (PCR), western blot and Elisa method, as well as Nuclear Magnetic Resonance (NMR) spectroscopy, were applied to unveil changes in the signal transduction pathways involved in osteosarcoma cells survival. The observed alterations in gene, protein and metabolite levels denote that OLEU not only inhibits MG-63 cells proliferation and potentiates ADR’s cytotoxicity, but also exerts its action, at least in part, through the induction of autophagy.

scholarly journals Ursolic acid inhibits proliferation and induces apoptosis by inactivating Wnt/β-catenin signaling in human osteosarcoma cells

2016 ◽  
Vol 49 (5) ◽  
pp. 1973-1982 ◽  
Author(s):  
Ran-Xi Zhang ◽  
Yang Li ◽  
Dong-Dong Tian ◽  
Yang Liu ◽  
Wu Nian ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells
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