scholarly journals Morphological and Spectroscopic Study of an Apatite Layer Induced by Fast-Set Versus Regular-Set EndoSequence Root Repair Materials

Materials ◽  
2019 ◽  
Vol 12 (22) ◽  
pp. 3678 ◽  
Author(s):  
Sawsan T. Abu Zeid ◽  
Ruaa A. Alamoudi ◽  
Ensanya A. Abou Neel ◽  
Abeer A. Mokeem Saleh
Keyword(s):  
Root Canal ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Apatite Layer ◽  
Material Surface ◽  
Apatite Formation ◽  
X Ray ◽  
Root Repair ◽  
Repair Materials ◽  
Root Canal Dentin

This study aimed to evaluate the morphology and chemistry of an apatite layer induced by fast-set versus regular-set EndoSequence root repair materials using spectroscopic analysis. Holes of a 4 mm diameter were created in the root canal dentin, which were filled with the test material. Fetal calf serum was used as the incubation medium, and the samples incubated in deionized water were used as controls. The material-surface and material-dentin interfaces were analyzed after 28 days using Raman and infrared spectroscopy, scanning electron microscopy/energy dispersive X-ray, and X-ray diffraction. After incubation in fetal calf serum, both materials formed a uniform layer of calcium phosphate precipitate on their surfaces, with the dentinal interface. This precipitated layer was a combination of hydroxyapatite and calcite or aragonite, and had a high mineral maturity with the regular-set paste. However, its crystallinity index was high with the fast-set putty. Typically, both consistencies (putty and paste) of root repair material have an apatite formation ability when they are incubated in fetal calf serum. This property could be beneficial in improving their sealing ability for root canal dentin.

scholarly journals Chemistry and Bioactivity of NeoMTA Plus™ versus MTA Angelus® Root Repair Materials

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Sawsan T. Abu Zeid ◽  
Najlaa M. Alamoudi ◽  
Monazah G. Khafagi ◽  
Ensanya A. Abou Neel
Keyword(s):  
Particle Size ◽  
Apatite Formation ◽  
X Ray Diffraction ◽  
Large Particle Size ◽  
Globular Structure ◽  
X Ray ◽  
Spherical Structure ◽  
Root Repair ◽  
Repair Materials ◽  
High Aluminium

Objectives. To analyse the chemistry and bioactivity of NeoMTA Plus in comparison with the conventional root repair materials. Method and Materials. Unhydrated and hydrated (initial and final sets) materials were analysed by Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). For bioactivity study, small holes of dentin discs were filled with either materials, immersed in PBS for 15 days, and analysed with FTIR and scanning electron microscope with energy dispersive X-ray (SEM/EDX). The calculation of crystallinity and carbonate/phosphate (CO3/PO4) ratio of surface precipitates (from FTIR) and calcium/phosphate (Ca/P) ratio (from EDX) was statistically analysed using t-test or ANOVA, respectively, at 0.05 significance. Results. Both materials are tricalcium silicate-based that finally react to be calcium silicate hydrate. NeoMTA Plus has relatively high aluminium and sulfur content, with tantalum oxide as an opacifier instead of zirconium oxide in MTA Angelus. NeoMTA Plus showed better apatite formation, higher crystallinity and Ca/P but lower CO3/PO4 ratio than MTA Angelus. SEM showed globular structure with a small particle size in NeoMTA Plus while spherical structure with large particle size in MTA Angelus. Conclusion. Due to fast setting, higher crystallinity, and better bioactivity of NeoMTA Plus, it can be used as a pulp and root repair material.

Composition and Surface Topography Effects on Apatite-Forming Ability of Ceramic-Polymer Composites

2003 ◽  
Vol 774 ◽  
Author(s):  
Susan M. Rea ◽  
Serena M. Best ◽  
William Bonfield
Keyword(s):  
Surface Topography ◽  
High Density Polyethylene ◽  
High Density ◽  
Apatite Layer ◽  
Biologically Active ◽  
Human Blood Plasma ◽  
Apatite Formation ◽  
Polyethylene Matrix ◽  
Composite Surface ◽  
X Ray

AbstractHAPEXTM (40 vol% hydroxyapatite in a high-density polyethylene matrix) and AWPEX (40 vol% apatite-wollastonite glass ceramic in a high density polyethylene matrix) are composites designed to provide bioactivity and to match the mechanical properties of human cortical bone. HAPEXTM has had clinical success in middle ear and orbital implants, and there is great potential for further orthopaedic applications of these materials. However, more detailed in vitro investigations must be performed to better understand the biological interactions of the composites and so the bioactivity of each material was assessed in this study. Specifically, the effects of controlled surface topography and ceramic filler composition on apatite layer formation in acellular simulated body fluid (SBF) with ion concentration similar to those of human blood plasma were examined. Samples were prepared as 1 cm × 1 cm × 1 mm tiles with polished, roughened, or parallel-grooved surface finishes, and were incubated in 20 ml of SBF at 36.5 °C for 1, 3, 7, or 14 days. The formation of a biologically active apatite layer on the composite surface after immersion was demonstrated by thin-film x-ray diffraction (TF-XRD), environmental scanning electron microscopy (ESEM) imaging and energy dispersive x-ray (EDX) analysis. Variations in sample weight and solution pH over the period of incubation were also recorded. Significant differences were found between the two materials tested, with greater bioactivity in AWPEX than HAPEXTM overall. Results also indicate that within each material the surface topography is highly important, with rougher samples correlated to earlier apatite formation.

Investigation of mineral content of root canal dentin after the application of various antibiotic paste using energy‐dispersive X‐ray detector

2018 ◽  
Vol 82 (2) ◽  
pp. 144-148 ◽  
Author(s):  
H. Simsek ◽  
M. Coruh ◽  
F. Cakici ◽  
F. Fundaoglu Kucukekenci ◽  
T. Gurbuz ◽  
...  
Keyword(s):  
Root Canal ◽  
Mineral Content ◽  
Energy Dispersive ◽  
X Ray ◽  
Root Canal Dentin

Evaluation of Root Canal Dentin Erosion after Different Irrigation Methods Using Energy-dispersive X-ray Spectroscopy

2016 ◽  
Vol 42 (12) ◽  
pp. 1834-1839 ◽  
Author(s):  
Zhejun Wang ◽  
Hazuki Maezono ◽  
Ya Shen ◽  
Markus Haapasalo
Keyword(s):  
Root Canal ◽  
Energy Dispersive ◽  
X Ray ◽  
Irrigation Methods ◽  
Root Canal Dentin ◽  
Dentin Erosion

Fast Apatite-Forming Ability of Magnetron Co-Sputtered Silicon-Containing Hydroxyapatite (Si-HA) Thin Films

2005 ◽  
Vol 284-286 ◽  
pp. 445-448 ◽  
Author(s):  
E.S. Thian ◽  
Jie Huang ◽  
Serena Best ◽  
Zoe H. Barber ◽  
William Bonfield
Keyword(s):  
Thin Films ◽  
Time Frame ◽  
Apatite Layer ◽  
Apatite Formation ◽  
The Novel ◽  
Processing Conditions ◽  
X Ray Diffraction ◽  
X Ray ◽  
And Control ◽  
Bone Apatite

0.8 wt.% silicon-containing hydroxyapatite (Si-HA) thin films of thickness 600 nm have been successfully developed using a magnetron co-sputtering technique, through careful selection and control of the processing conditions. These films were immersed in simulated body fluid (SBF) to investigate the nucleation and growth of an apatite layer on their surfaces. A newly-formed apatite layer with similar characteristics to that of the biological bone apatite, was observed after 4 days of immersion in SBF. X-ray diffraction and infrared analyses confirmed this layer to be calciumdeficient micro-crystalline carbonate HA. These results demonstrated that the novel Si-HA films were highly bioactive and the time frame required for apatite formation was reduced by approximately 76 % (from 17 days to 4 days).

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

Fish kidney cell line in response to heat shock

1992 ◽  
Vol 50 (1) ◽  
pp. 704-705
Author(s):  
Li-Chu Tung ◽  
Yung-Reui Chen ◽  
Shiu-Nan Chen ◽  
Guang-Hsiung Kuo
Keyword(s):  
Heat Shock ◽  
Cell Line ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Uranyl Acetate ◽  
Ultrastructural Changes ◽  
Heat Shock Treatment ◽  
Acanthopagrus Schlegeli ◽  
Cacodylate Buffer ◽  
Fish Kidney

In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli, under heat shock treatment are described.The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV.

scholarly journals Assessment of Titanate Nanolayers in Terms of Their Physicochemical and Biological Properties

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 806
Author(s):  
Michalina Ehlert ◽  
Aleksandra Radtke ◽  
Katarzyna Roszek ◽  
Tomasz Jędrzejewski ◽  
Piotr Piszczek
Keyword(s):  
Surface Modification ◽  
Substrate Surface ◽  
Biological Properties ◽  
Structure Characterization ◽  
Porous Coating ◽  
Apatite Formation ◽  
Sem Images ◽  
Energy Dispersion Spectroscopy ◽  
X Ray

The surface modification of titanium substrates and its alloys in order to improve their osseointegration properties is one of widely studied issues related to the design and production of modern orthopedic and dental implants. In this paper, we discuss the results concerning Ti6Al4V substrate surface modification by (a) alkaline treatment with a 7 M NaOH solution, and (b) production of a porous coating (anodic oxidation with the use of potential U = 5 V) and then treating its surface in the abovementioned alkaline solution. We compared the apatite-forming ability of unmodified and surface-modified titanium alloy in simulated body fluid (SBF) for 1–4 weeks. Analysis of the X-ray diffraction patterns of synthesized coatings allowed their structure characterization before and after immersing in SBF. The obtained nanolayers were studied using Raman spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy (DRIFT), and scanning electron microscopy (SEM) images. Elemental analysis was carried out using X-ray energy dispersion spectroscopy (SEM EDX). Wettability and biointegration activity (on the basis of the degree of integration of MG-63 osteoblast-like cells, L929 fibroblasts, and adipose-derived mesenchymal stem cells cultured in vitro on the sample surface) were also evaluated. The obtained results proved that the surfaces of Ti6Al4V and Ti6Al4V covered by TiO2 nanoporous coatings, which were modified by titanate layers, promote apatite formation in the environment of body fluids and possess optimal biointegration properties for fibroblasts and osteoblasts.

scholarly journals Fetal calf serum contains activities that induce fetal hemoglobin in adult erythroid cell cultures

Blood ◽  
1990 ◽  
Vol 75 (9) ◽  
pp. 1862-1869 ◽  
Author(s):  
P Constantoulakis ◽  
B Nakamoto ◽  
T Papayannopoulou ◽  
G Stamatoyannopoulos
Keyword(s):  
Cell Cultures ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Fetal Hemoglobin ◽  
Erythroid Cell ◽  
Erythroid Progenitors ◽  
Globin Mrna ◽  
Erythroid Progenitor ◽  
Fetal Sheep ◽  
Gamma Globin

Abstract Cultures of peripheral blood or bone marrow erythroid progenitors display stimulated production of fetal hemoglobin. We investigated whether this stimulation is due to factors contained in the sera of the culture medium. Comparisons of gamma/gamma + beta biosynthetic ratios in erythroid colonies grown in fetal calf serum (FCS) or in charcoal treated FCS (C-FCS) showed that FCS-grown cells had significantly higher gamma/gamma + beta ratios. This increase in globin chain biosynthesis was reflected by an increase in relative amounts of steady- state gamma-globin mRNA. In contrast to its effect on adult cells, FCS failed to influence gamma-chain synthesis in fetal burst forming units- erythroid (BFU-E) colonies. There was a high correlation of gamma- globin expression in paired cultures done with C-FCS or fetal sheep serum. Dose-response experiments showed that the induction of gamma- globin expression is dependent on the concentration of FCS. These results indicate that FCS contains an activity that induces gamma- globin expression in adult erythroid progenitor cell cultures.

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