scholarly journals Human Stem Cell Responses and Surface Characteristics of 3D Printing Co-Cr Dental Material

Materials ◽  
2019 ◽  
Vol 12 (20) ◽  
pp. 3419 ◽  
Author(s):  
Boldbayar Ganbold ◽  
Seong-Joo Heo ◽  
Jai-Young Koak ◽  
Seong-Kyun Kim ◽  
Jaejin Cho
Keyword(s):  
Surface Roughness ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Three Dimensional ◽  
Surface Characteristics ◽  
Water Soluble ◽  
Tetrazolium Salt ◽  
Casting Method ◽  
Conventional Casting ◽  
3D Printed

Recently, the selective laser melting (SLM) method of manufacturing three dimensional (3D) dental prosthetics by applying a laser to metal powder has been widely used in the field of dentistry. This study investigated human adipose derived stem cell (hADSC) behavior on a 3D printed cobalt-chrome (Co-Cr) alloy and its surface characteristics and compared them those of a nickel-chrome (Ni-Cr) alloy. Alloys were divided into four groups according to the material and manufacturing methods. Co-Cr disks were manufactured with three different methods: a conventional casting method, a metal milling method, and an SLM method. Ni-Cr disks were manufactured with a conventional casting method. The surface roughness and compositions of the disks were assessed. hADSCs were then cultured on the disks. Cell morphologies on the disks were analyzed by a field emission scanning electron microscope (FE-SEM). Cell proliferation was assessed with a bromodeoxyuridine (BrdU) assay kit. Cell viability was evaluated with a water-soluble tetrazolium salt (WST) assay kit. There were no differences in surface roughness between all groups. The cells were well attached to the disks, and morphologies of the cells were similar. The cell proliferation and viability of the Ni-Cr disks were significantly lower than the other groups. However, the Co-Cr disks showed no differences in their different fabricating methods. In conclusion, the biocompatibility of 3D printed Co-Cr alloys showed comparable results compared to that of the conventional casting method, and these alloys were more biocompatible than Ni-Cr alloys.

scholarly journals 3D-Printed Poly(ε-Caprolactone)/Hydroxyapatite Scaffolds Modified with Alkaline Hydrolysis Enhance Osteogenesis In Vitro

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 257
Author(s):  
Sangbae Park ◽  
Jae Eun Kim ◽  
Jinsub Han ◽  
Seung Jeong ◽  
Jae Woon Lim ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Surface Characteristics ◽  
Water Soluble ◽  
Great Promise ◽  
Tetrazolium Salt ◽  
Water Contact ◽  
Alizarin Red ◽  
3D Printed ◽  
O2 Plasma

The 3D-printed bioactive ceramic incorporated Poly(ε-caprolactone) (PCL) scaffolds show great promise as synthetic bone graft substitutes. However, 3D-printed scaffolds still lack adequate surface properties for cells to be attached to them. In this study, we modified the surface characteristics of 3D-printed poly(ε-caprolactone)/hydroxyapatite scaffolds using O2 plasma and sodium hydroxide. The surface property of the alkaline hydrolyzed and O2 plasma-treated PCL/HA scaffolds were evaluated using field-emission scanning microscopy (FE-SEM), Alizarin Red S (ARS) staining, and water contact angle analysis, respectively. The in vitro behavior of the scaffolds was investigated using human dental pulp-derived stem cells (hDPSCs). Cell proliferation of hDPSCs on the scaffolds was evaluated via immunocytochemistry (ICC) and water-soluble tetrazolium salt (WST-1) assay. Osteogenic differentiation of hDPSCs on the scaffolds was further investigated using ARS staining and Western blot analysis. The result of this study shows that alkaline treatment is beneficial for exposing hydroxyapatite particles embedded in the scaffolds compared to O2 plasma treatment, which promotes cell proliferation and differentiation of hDPSCs.

Toxicity and Cell Density Monitoring in Monolayer and Three-dimensional Cultures with the XTT Assay

1998 ◽  
Vol 26 (3) ◽  
pp. 331-342
Author(s):  
Xavier Ponsoda ◽  
Maria Jose Gómez-Lechón ◽  
Jose V. Castell
Keyword(s):  
Cell Proliferation ◽  
Colorimetric Assay ◽  
Three Dimensional ◽  
Cellular Protein ◽  
Water Soluble ◽  
Tetrazolium Salt ◽  
Collagen Gels ◽  
Xtt Assay ◽  
Cellular Density ◽  
Monolayer Cultures

The application of viability criteria (MTT and XTT tests) to monolayer cultures and immobilised cells in three-dimensional systems was investigated in order to assess cell viability and cell proliferation. The suitability and accuracy of these tests were compared with the conventional criteria (cellular protein and DNA content) used in monolayer cultures for the same purpose. The colorimetric assay based on the metabolic reduction of the tetrazolium salt XTT to a water-soluble formazan proved to be very useful, rapid and sensitive. This automated spectrophotometric enzymatic method, due to its lack of toxicity, also permits repeated nondestructive assays on a single cellular culture for the long-term monitoring of cytotoxicity, cell survival and cell proliferation, and can be performed in 96-well plates with minimal handling. This method could offer a solution for cellular density evaluation in complex cell cultures that do not permit visual examination; it is also the best choice for protein-based, three-dimensional systems such as collagen gels.

scholarly journals Dimensional Accuracy of Dental Models for Three-Unit Prostheses Fabricated by Various 3D Printing Technologies

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1550
Author(s):  
Soo-Yeon Yoo ◽  
Seong-Kyun Kim ◽  
Seong-Joo Heo ◽  
Jai-Young Koak ◽  
Joung-Gyu Kim
Keyword(s):  
Surface Roughness ◽  
Three Dimensional ◽  
Dimensional Accuracy ◽  
3D Analysis ◽  
Digital Light Processing ◽  
Test Models ◽  
Reference File ◽  
Significant Difference ◽  
Jet Printing ◽  
3D Printed

Previous studies on accuracy of three-dimensional (3D) printed model focused on full arch measurements at few points. The aim of this study was to examine the dimensional accuracy of 3D-printed models which were teeth-prepped for three-unit fixed prostheses, especially at margin and proximal contact areas. The prepped dental model was scanned with a desktop scanner. Using this reference file, test models were fabricated by digital light processing (DLP), Multi-Jet printing (MJP), and stereo-lithography apparatus (SLA) techniques. We calculated the accuracy (trueness and precision) of 3D-printed models on 3D planes, and deviations of each measured points at buccolingual and mesiodistal planes. We also analyzed the surface roughness of resin printed models. For overall 3D analysis, MJP showed significantly higher accuracy (trueness) than DLP and SLA techniques; however, there was not any statistically significant difference on precision. For deviations on margins of molar tooth and distance to proximal contact, MJP showed significantly accurate results; however, for a premolar tooth, there was no significant difference between the groups. 3D color maps of printed models showed contraction buccolingually, and surface roughness of the models fabricated by MJP technique was observed as the lowest. The accuracy of the 3D-printed resin models by DLP, MJP, and SLA techniques showed a clinically acceptable range to use as a working model for manufacturing dental prostheses

scholarly journals Surface Functionalization of Three Dimensional-Printed Polycaprolactone-Bioactive Glass Scaffolds by Grafting GelMA Under UV Irradiation

2020 ◽  
Vol 7 ◽  
Author(s):  
Farnaz Ghorbani ◽  
Melika Sahranavard ◽  
Zohre Mousavi Nejad ◽  
Dejian Li ◽  
Ali Zamanian ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Surface Roughness ◽  
Surface Modification ◽  
Bioactive Glass ◽  
Uv Irradiation ◽  
Three Dimensional ◽  
Homogeneous Distribution ◽  
X Ray ◽  
Fused Deposition ◽  
3D Printed

In this study, bioactive glass nanoparticles (BGNPs) with an average diameter of less than 10 nm were synthesized using a sol-gel method and then characterized by transmission electron microscopy (TEM), differential scanning calorimetric (DSC), Fourier transforms infrared spectroscopy (FTIR), and x-ray spectroscopy (XRD). Afterward, three dimensional (3D)-printed polycaprolactone (PCL) scaffolds along with fused deposition modeling (FDM) were incorporated with BGNPs, and the surface of the composite constructs was then functionalized by coating with the gelatin methacryloyl (GelMA) under UV irradiation. Field emission scanning electron microscopy micrographs demonstrated the interconnected porous microstructure with an average pore diameter of 260 µm and homogeneous distribution of BGNPs. Therefore, no noticeable shrinkage was observed in 3D-printed scaffolds compared with the computer-designed file. Besides, the surface was uniformly covered by GelMA, and no effect of surface modification was observed on the microstructure while surface roughness increased. The addition of the BGNPs the to PCL scaffolds showed a slight change in pore size and porosity; however, it increased surface roughness. According to mechanical analysis, the compression strength of the scaffolds was increased by the BGNPs addition and surface modification. Also, a reduction was observed in the absorption capacity and biodegradation of scaffolds in phosphate-buffered saline media after the incorporation of BGNPs, while the presence of the GelMA layer increased the swelling potential and stability of the composite matrixes. Moreover, the capability of inducing bio-mineralization of hydroxyapatite-like layers, as a function of BGNPs content, was proven by FE-SEM micrographs, EDX spectra, and x-ray diffraction spectra (XRD) after soaking the obtained samples in concentrated simulated body fluid. A higher potential of the modified constructs to interact with the aqueous media led to better precipitation of minerals. According to in-vitro assays, the modified scaffolds can provide a suitable surface for the attachment and spreading of the bone marrow mesenchymal stem cells (BMSCs). Furthermore, the number of the proliferated cells confirms the biocompatibility of the scaffolds, especially after a modification process. Cell differentiation was verified by alkaline phosphatase activity as well as the expression of osteogenic genes such as osteocalcin and osteopontin. Accordingly, the scaffolds showed an initial potential for reconstruction of the injured bone.

scholarly journals Surface Characteristics of Milled and 3D Printed Denture Base Materials Following Polishing and Coating: An In-Vitro Study

Materials ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3305
Author(s):  
Pablo Kraemer Fernandez ◽  
Alexey Unkovskiy ◽  
Viola Benkendorff ◽  
Andrea Klink ◽  
Sebastian Spintzyk
Keyword(s):  
Surface Roughness ◽  
3D Printing ◽  
In Vitro Study ◽  
Surface Characteristics ◽  
Denture Base ◽  
Liquid Resin ◽  
Base Materials ◽  
Superior Surface ◽  
3D Printed

(1) Background: To date, no information on the polishability of milled and 3D-printed complete denture bases has been provided, which is relevant in terms of plaque accumulation. (2) Methods: three groups (n = 30) were manufactured using the cold-polymerization polymethilmethacrilate, milling (SM) and 3D printing (AM). 10 specimens of each group were left untreated (reference). 10 more specimens were pre-polished (intermediate polishing) and 10 final specimens were highgloss polished. An additional 20 specimens were 3D printed and coated with the liquid resin (coated), 10 of which were additionally polished (coated + polished). For each group Ra and Rz values, gloss value and REM images were obtained. (3). The “highgloss-polished” specimens showed statistically lower Ra and Rz values in the SM, followed by AM and conventional groups. In the AM group statistically lower surfaces roughness was revealed for highgloss-polished, “coated + polished”, and “coated” specimens, respectively. (4) Conclusions: The milled specimens demonstrated superiors surface characteristics than 3D printed and conventionally produced after polishing. The polished specimens demonstrated superior surface characteristics over coated specimens. However, the surface roughness by both polished and coated specimens was within the clinically relevant threshold of 0.2 µm.

A Three-Dimensional Fractal Analysis Method for Ground Monocrystal Sapphire Surface

2014 ◽  
Vol 1017 ◽  
pp. 187-192
Author(s):  
Qiu Yan Wang ◽  
Zhi Qiang Liang ◽  
Xi Bin Wang ◽  
Wen Xiang Zhao ◽  
Yong Bo Wu ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Fractal Dimension ◽  
Fractal Analysis ◽  
Three Dimensional ◽  
Ground Surface ◽  
Surface Characteristics ◽  
Surface Phenomenon ◽  
Analysis Method ◽  
Characterization Methods ◽  
Box Counting

Conventional characterization methods of grinding surface using surface roughness parameters, e.g., Ra, depend on either the resolution of the measuring instrument or the length of the sample. But fractal dimension (FD) as a scale-independent fractal parameter is effective to evaluate the ground surface at any length scale and represent lots of surface phenomenon at its relevant length scales. In this paper, a three-dimensional (3D) box-counting fractal analysis method is used to investigate ground surface morphology of monocrystal sapphire by calculating 3D fractal dimension of the ground surface. The results obtained show that fractal dimension decreases with the increasing surface roughness. For the ground surface with higher fractal dimension, its microtopography is more exquisite with minor defects. Once the fractal dimension become smaller, deep cracks and pronounced defects are exhibited in ground surface. Moreover, the ground surface obtained in ductile mode has much higher fractal dimension than that in brittle mode. Therefore, the fractal analysis method has the potential to reveal the ground surface characteristics of monocrystal sapphire.

scholarly journals Pirfenidone Inhibits Cell Proliferation and Collagen I Production of Primary Human Intestinal Fibroblasts

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 775 ◽  
Author(s):  
Yingying Cui ◽  
Mengfan Zhang ◽  
Changsen Leng ◽  
Tjasso Blokzijl ◽  
Bernadien H. Jansen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Type I Collagen ◽  
Water Soluble ◽  
Collagen I ◽  
Brdu Incorporation ◽  
Tetrazolium Salt ◽  
Type I ◽  
Mrna Levels ◽  
Intestinal Fibrosis ◽  
Tgf Β1

Intestinal fibrosis is a common complication of inflammatory bowel disease. So far, there is no safe and effective drug for intestinal fibrosis. Pirfenidone is an anti-fibrotic compound available for the treatment of idiopathic pulmonary fibrosis. Here, we explored the anti-proliferative and anti-fibrotic properties of pirfenidone on primary human intestinal fibroblasts (p-hIFs). p-hIFs were cultured in the absence and presence of pirfenidone. Cell proliferation was measured by a real-time cell analyzer (xCELLigence) and BrdU incorporation. Cell motility was monitored by live cell imaging. Cytotoxicity and cell viability were analyzed by Sytox green, Caspase-3 and Water Soluble Tetrazolium Salt-1 (WST-1) assays. Gene expression of fibrosis markers was determined by quantitative reverse transcription PCR (RT-qPCR). The mammalian target of rapamycin (mTOR) signaling was analyzed by Western blotting and type I collagen protein expression additionally by immunofluorescence microscopy. Pirfenidone dose-dependently inhibited p-hIF proliferation and motility, without inducing cell death. Pirfenidone suppressed mRNA levels of genes that contribute to extracellular matrix production, as well as basal and TGF-β1-induced collagen I protein production, which was associated with inhibition of the rapamycin-sensitive mTOR/p70S6K pathway in p-hIFs. Thus, pirfenidone inhibits the proliferation of intestinal fibroblasts and suppresses collagen I production through the TGF-β1/mTOR/p70S6K signaling pathway, which might be a novel and safe anti-fibrotic strategy to treat intestinal fibrosis.

scholarly journals Comparative study of hyperpure chlorine dioxide with two other irrigants regarding the viability of periodontal ligament stem cells

2020 ◽  
Author(s):  
Orsolya Láng ◽  
Krisztina S. Nagy ◽  
Julia Láng ◽  
Katalin Perczel-Kovách ◽  
Anna Herczegh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Viability ◽  
Chlorine Dioxide ◽  
Periodontal Ligament ◽  
Water Soluble ◽  
Tetrazolium Salt ◽  
Stem Cell Markers ◽  
Periodontal Ligament Stem Cells ◽  
Cell Markers

Abstract Objectives Periodontal ligament stem cells (PDLSCs) have an underlined significance as their high proliferative capacity and multipotent differentiation provide an important therapeutic potential. The integrity of these cells is frequently disturbed by the routinely used irrigative compounds applied as periodontal or endodontic disinfectants (e.g., hydrogen peroxide (H2O2) and chlorhexidine (CHX)). Our objectives were (i) to monitor the cytotoxic effect of a novel dental irrigative compound, chlorine dioxide (ClO2), compared to two traditional agents (H2O2, CHX) on PDLSCs and (ii) to test whether the aging factor of PDLSC cultures determines cellular responsiveness to the chemicals tested. Methods Impedimetry (concentration-response study), WST-1 assays (WST = water soluble tetrazolium salt), and morphology analysis were performed to measure changes in cell viability induced by the 3 disinfectants; immunocytochemistry of stem cell markers (STRO-1, CD90, and CD105) measured the induced mesenchymal characteristics. Results Cell viability experiments demonstrated that the application of ClO2 does not lead to a significant decrease in viability of PLDSCs in concentrations used to kill microbes. On the contrary, traditional irrigants, H2O2, and CHX are highly toxic on PDLSCs. Aging of PLDSC cultures (passages 3 vs. 7) has characteristic effects on their responsiveness to these agents as the increased expression of mesenchymal stem cell markers turns to decreased. Conclusions and clinical relevance While the active ingredients of mouthwash (H2O2, CHX) applied in endodontic or periodontitis management have a serious toxic effect on PDLSCs, the novel hyperpure ClO2 is less toxic providing an environment favoring dental structure regenerations during disinfectant interventions.

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