scholarly journals Oral Bone Tissue Engineering: Advanced Biomaterials for Cell Adhesion, Proliferation and Differentiation

Materials ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2296 ◽  
Author(s):  
Alexandra Roi ◽  
Lavinia Cosmina Ardelean ◽  
Ciprian Ioan Roi ◽  
Eugen-Radu Boia ◽  
Simina Boia ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Surface Characteristics ◽  
Future Research ◽  
Biomaterial Scaffold ◽  
Proliferation And Differentiation ◽  
Biomaterial Scaffolds ◽  
Oral Tissue ◽  
Advanced Biomaterials

The advancements made in biomaterials have an important impact on oral tissue engineering, especially on the bone regeneration process. Currently known as the gold standard in bone regeneration, grafting procedures can sometimes be successfully replaced by a biomaterial scaffold with proper characteristics. Whether natural or synthetic polymers, biomaterials can serve as potential scaffolds with major influences on cell adhesion, proliferation and differentiation. Continuous research has enabled the development of scaffolds that can be specifically designed to replace the targeted tissue through changes in their surface characteristics and the addition of growth factors and biomolecules. The progress in tissue engineering is incontestable and research shows promising contributions to the further development of this field. The present review aims to outline the progress in oral tissue engineering, the advantages of biomaterial scaffolds, their direct implication in the osteogenic process and future research directions.

scholarly journals Surface modification of small intestine submucosa in tissue engineering

2020 ◽  
Vol 7 (4) ◽  
pp. 339-348 ◽  
Author(s):  
Pan Zhao ◽  
Xiang Li ◽  
Qin Fang ◽  
Fanglin Wang ◽  
Qiang Ao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Small Intestine ◽  
Cell Adhesion ◽  
Great Influence ◽  
Small Intestine Submucosa ◽  
Proliferation And Differentiation ◽  
Tissue Reconstruction

Abstract With the development of tissue engineering, the required biomaterials need to have the ability to promote cell adhesion and proliferation in vitro and in vivo. Especially, surface modification of the scaffold material has a great influence on biocompatibility and functionality of materials. The small intestine submucosa (SIS) is an extracellular matrix isolated from the submucosal layer of porcine jejunum, which has good tissue mechanical properties and regenerative activity, and is suitable for cell adhesion, proliferation and differentiation. In recent years, SIS is widely used in different areas of tissue reconstruction, such as blood vessels, bone, cartilage, bladder and ureter, etc. This paper discusses the main methods for surface modification of SIS to improve and optimize the performance of SIS bioscaffolds, including functional group bonding, protein adsorption, mineral coating, topography and formatting modification and drug combination. In addition, the reasonable combination of these methods also offers great improvement on SIS surface modification. This article makes a shallow review of the surface modification of SIS and its application in tissue engineering.

Importance of Poly(lactic-co-glycolic acid) in Scaffolds for Guided Bone Regeneration: A Focused Review

2015 ◽  
Vol 41 (4) ◽  
pp. e152-e157 ◽  
Author(s):  
Gabriel Castillo-Dalí ◽  
Rocío Velázquez-Cayón ◽  
M. Angeles Serrera-Figallo ◽  
Agustín Rodríguez-González-Elipe ◽  
José-Luis Gutierrez-Pérez ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Repair ◽  
Glycolic Acid ◽  
Guided Bone Regeneration ◽  
Future Research ◽  
Loss Of Function ◽  
Important Health

Total or partial tissue damage and loss of function in an organ are two of the most serious and costly issues in human health. Initially, these problems were approached through organ and allogenic tissue transplantation, but this option is limited by the scarce availability of donors. In this manner, new bone for restoring or replacing lost and damaged bone tissue is an important health and socioeconomic necessity. Tissue engineering has been used as a strategy during the 21st century for mitigating this need through the development of guided bone regeneration scaffold and composites. In this manner, compared with other traditional methods, bone tissue engineering offers a new and interesting approach to bone repair. The poly-α-hydroxy acids, which include the copolymers of lactic acid and glycolic acid, have been used commonly in the fabrication of these scaffolds. The objective of our article was to review the characteristics and functions of scaffold with biomedical applications, with special interest in scaffold construction using poly(lactic-co-glycolic acid) polymers, in order to update the current methods used for fabrication and to improve the quality of these scaffolds, integrating this information into the context of advancements made in tissue engineering based on these structures. In the future, research into bone regeneration should be oriented toward a fruitful exchange between disciplines involved in tissue engineering, which is coming very close to filling the gaps in our ability to provide implants and restoration of functionality in bone tissue. Overcoming this challenge will provide benefits to a major portion of the population and facilitate substantial improvements to quality of life.

Demineralized and decellularized bone extracellular matrix incorporated electrospun nanofibrous scaffold for bone regeneration

2021 ◽  
Author(s):  
Chanjuan Dong ◽  
Fangyu Qiao ◽  
Guobao Chen ◽  
Yonggang Lv
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Nanofibrous Scaffold ◽  
Proliferation And Differentiation

The extracellular matrix (ECM)-based materials has been employed as scaffolds for bone tissue engineering, providing a suitable microenvironment that possesses biophysical and biochemical cues for cell attachment, proliferation and differentiation....

scholarly journals Carbon Nanomaterials for Electro-Active Structures: A Review

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2946
Author(s):  
Weiguang Wang ◽  
Yanhao Hou ◽  
Dean Martinez ◽  
Darwin Kurniawan ◽  
Wei-Hung Chiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanomaterials ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Future Research ◽  
Electrically Conductive ◽  
Active Structures ◽  
Proliferation And Differentiation ◽  
Conductive Property ◽  
Materials Used

The use of electrically conductive materials to impart electrical properties to substrates for cell attachment proliferation and differentiation represents an important strategy in the field of tissue engineering. This paper discusses the concept of electro-active structures and their roles in tissue engineering, accelerating cell proliferation and differentiation, consequently leading to tissue regeneration. The most relevant carbon-based materials used to produce electro-active structures are presented, and their main advantages and limitations are discussed in detail. Particular emphasis is put on the electrically conductive property, material synthesis and their applications on tissue engineering. Different technologies, allowing the fabrication of two-dimensional and three-dimensional structures in a controlled way, are also presented. Finally, challenges for future research are highlighted. This review shows that electrical stimulation plays an important role in modulating the growth of different types of cells. As highlighted, carbon nanomaterials, especially graphene and carbon nanotubes, have great potential for fabricating electro-active structures due to their exceptional electrical and surface properties, opening new routes for more efficient tissue engineering approaches.

scholarly journals Fabrication and Characterization of Collagen-Immobilized Porous PHBV/HA Nanocomposite Scaffolds for Bone Tissue Engineering

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Jin-Young Baek ◽  
Zhi-Cai Xing ◽  
Giseop Kwak ◽  
Keun-Byoung Yoon ◽  
Soo-Young Park ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Collagen Type I ◽  
Type I ◽  
Hot Press ◽  
Composite Scaffolds ◽  
Salt Leaching ◽  
Proliferation And Differentiation ◽  
Nanocomposite Scaffolds

The porous composite scaffolds (PHBV/HA) consisting of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and hydroxyapatite (HA) were fabricated using a hot-press machine and salt-leaching. Collagen (type I) was then immobilized on the surface of the porous PHBV/HA composite scaffolds to improve tissue compatibility. The structure and morphology of the collagen-immobilized composite scaffolds (PHBV/HA/Col) were investigated using a scanning electron microscope (SEM), Fourier transform infrared (FTIR), and electron spectroscopy for chemical analysis (ESCA). The potential of the porous PHBV/HA/Col composite scaffolds for use as a bone scaffold was assessed by an experiment with osteoblast cells (MC3T3-E1) in terms of cell adhesion, proliferation, and differentiation. The results showed that the PHBV/HA/Col composite scaffolds possess better cell adhesion and significantly higher proliferation and differentiation than the PHBV/HA composite scaffolds and the PHBV scaffolds. These results suggest that the PHBV/HA/Col composite scaffolds have a high potential for use in the field of bone regeneration and tissue engineering.

scholarly journals Scaffold-Mediated Immunoengineering as Innovative Strategy for Tendon Regeneration

Cells ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 266
Author(s):  
Valentina Russo ◽  
Mohammad El Khatib ◽  
Giuseppe Prencipe ◽  
Adrián Cerveró Varona ◽  
Maria Rita Citeroni ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Tendon Injuries ◽  
Bioactive Molecules ◽  
Technological Advancement ◽  
Innovative Strategy ◽  
Innovative Strategies ◽  
Tendon Regeneration ◽  
Biomaterial Scaffold ◽  
Biomaterial Scaffolds

Tendon injuries are at the frontier of innovative approaches to public health concerns and sectoral policy objectives. Indeed, these injuries remain difficult to manage due to tendon’s poor healing ability ascribable to a hypo-cellularity and low vascularity, leading to the formation of a fibrotic tissue affecting its functionality. Tissue engineering represents a promising solution for the regeneration of damaged tendons with the aim to stimulate tissue regeneration or to produce functional implantable biomaterials. However, any technological advancement must take into consideration the role of the immune system in tissue regeneration and the potential of biomaterial scaffolds to control the immune signaling, creating a pro-regenerative environment. In this context, immunoengineering has emerged as a new discipline, developing innovative strategies for tendon injuries. It aims at designing scaffolds, in combination with engineered bioactive molecules and/or stem cells, able to modulate the interaction between the transplanted biomaterial-scaffold and the host tissue allowing a pro-regenerative immune response, therefore hindering fibrosis occurrence at the injury site and guiding tendon regeneration. Thus, this review is aimed at giving an overview on the role exerted from different tissue engineering actors in leading immunoregeneration by crosstalking with stem and immune cells to generate new paradigms in designing regenerative medicine approaches for tendon injuries.

Boron Containing Nano Hydroxyapatites (B-n-HAp) Stimulate Mesenchymal Stem Cell Adhesion, Proliferation and Differentiation

2014 ◽  
Vol 631 ◽  
pp. 373-378 ◽  
Author(s):  
Eda Ciftci ◽  
Sevil Köse ◽  
Petek Korkusuz ◽  
Muharrem Timuçin ◽  
Feza Korkusuz
Keyword(s):  
Cell Adhesion ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Quantitative Methods ◽  
Fragility Fractures ◽  
Mineral Density ◽  
Differentiation Potential ◽  
Cell Index ◽  
Tissue Integration ◽  
Proliferation And Differentiation

Osteoporosis (OP) is a systemic metabolic disease identified with decrease of bone mineral density and deterioration of microstructure leading to fragility fractures in elderly. Boron (B) is assumed to stimulate osteoblasts. Hydroxyapatite (HAp) is clinically used to conduct bone regeneration and improves implant integration. Nano(n)-HAp expands the surface area for cell adhesion and may improve bone regeneration and tissue integration. The objective of this study was to examine the adhesion, proliferation and differentiation of B-n-HAp with mesenchymal stem cells (MSC’s). Human bone marrow derived MSC’s phenotype was assessed using scanning and transmission electron microscopy after combining with B-n-HAp and n-HAp. Cell adhesion and proliferation potential of these ceramics was examined with the real time cell analysis (xCELLigence, Roche Applied Science and ACEA Bioscience, USA) system and adipogenic-osteogenic differentiation was analyzed with morphological and quantitative methods. MSC’s adhesion and proliferation rates (cell index, 4.50) were higher than controls (cell index, 4.00). Adipogenic and osteogenic differentiation potential of MSC’s remained unchanged in the presence of B-n-HAp ceramics. In conclusion, B-n-HAp stimulates MSC’s adhesion, proliferation and differentiation and has a potential to regenerate bone tissue.

Poly-l-lysine-coated PLGA/poly(amino acid)-modified hydroxyapatite porous scaffolds as efficient tissue engineering scaffolds for cell adhesion, proliferation, and differentiation

2019 ◽  
Vol 43 (25) ◽  
pp. 9989-10002 ◽  
Author(s):  
Shuang Zheng ◽  
Yonghong Guan ◽  
Haichi Yu ◽  
Ge Huang ◽  
Changjun Zheng
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Amino Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Function ◽  
Porous Scaffolds ◽  
Tissue Engineering Scaffolds ◽  
Proliferation And Differentiation

Ideal bone tissue engineering scaffolds should be biocompatible, biodegradable, and mechanically robust and have the ability to regulate cell function.

Role of Hydrogels in Bone Tissue Engineering: How Properties Shape Regeneration

2020 ◽  
Vol 16 (12) ◽  
pp. 1667-1686
Author(s):  
Yanting Wu ◽  
Xu Zhang ◽  
Qian Zhao ◽  
Bowen Tan ◽  
Xingyu Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Clinical Problem ◽  
Allogeneic Transplantation ◽  
Research Field ◽  
Proliferation And Differentiation ◽  
Post Traumatic ◽  
Good Biocompatibility

Bone defect that resulted from trauma, tumors, and other reasons is believed as a common clinical problem, which exists mainly in post-traumatic healing. Additionally, autologous/allogeneic transplantation, bone tissue engineering attracts increasing attention due to the existing problem of the limited donor. The applications of biomaterials can be considered as a rising and promising strategy for bone regeneration. Especially, hydrogel is featured with hydrophilic characteristic, good biocompatibility, and porous structure, which shows unique properties for bone regeneration. The main properties of hydrogel such as surface property, adhesive property, mechanical property, porosity, and degradation property, generally present influences on the migration, proliferation, and differentiation of mesenchymal stem cells exclusively or in combination, which consequently affect the regeneration of bones. This review mainly focuses on the theme: "how properties of hydrogel shape bone regeneration." Moreover, the latest progress achieved in the above mentioned direction is further discussed. Despite the fascinating advances researchers have made, certain potential challenges continue to exist in the research field, which need to be addressed for accelerating the clinical translation of hydrogel in bone regeneration.

Surface Roughness Values Closer to Bone for Titania Nanoparticle/Poly-lactic-co-glycolic Acid (PLGA) Composites Increases Bone Cell Adhesion

2005 ◽  
Vol 873 ◽  
Author(s):  
Huinan Liu ◽  
Elliott B. Slamovich ◽  
Thomas J. Webster
Keyword(s):  
Tissue Engineering ◽  
Surface Roughness ◽  
Cell Adhesion ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Glycolic Acid ◽  
Ceramic Materials ◽  
Bone Substitutes ◽  
Bone Cell ◽  
Osteoblast Adhesion

AbstractBone substitutes are often required to replace damaged tissue due to injuries, diseases and genetic malformations. Traditional bone substitutes, such as autografts, allografts, xenografts and metal implants, are far from ideal as each have their own specific problems and limitations. Bone tissue engineering offers a promising opportunity for bone regeneration in a natural way. However, currently the scientific challenges of bone tissue engineering lie in the development of suitable scaffold materials that can improve bone cell adhesion, proliferation and differentiation. The design of nanophase titania/polymer composites offers an exciting approach to combine the advantages of a degradable polymer with nano-size ceramic grains that optimize biological properties for bone regeneration. Importantly, nanophase titania mimics the size scale of constituent components of bone since bone itself is a nanostructured composite composed of nanometer hydroxyapatite crystals well-dispersed in a mostly collagen matrix. Previous studies have shown significant improvement in protein adsorption, osteoblast (bone-forming cell) adhesion and long-term functions on nano-grain ceramic materials compared to traditional micron-grain ceramic materials. This study used nanometer grain size titania dispersed in a model polymer (PLGA or poly-lactic-co-glycolic acid) matrix by using various sonication powers to increase osteoblast adhesion. The surface characteristics of the composites, such as topography, titania surface area coverage and surface roughness, were studied by scanning electron microscopy and atomic force microscopy. Of all the composites formulated in this study, osteoblast adhesion was the greatest on nanophase titania/PLGA (30/70 wt.%) sonicated at 118.75 for 10 minutes; this composite was the closest in terms of nanometer surface roughness compared to bone of all the composites formulated. In this manner, this study suggests that nanophase titania sonicated in PLGA under these conditions should be further studied for orthopedic applications.

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