scholarly journals Biodentine™ Boosts, WhiteProRoot®MTA Increases and Life® Suppresses Odontoblast Activity

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1184 ◽  
Author(s):  
Anabela Paula ◽  
Mafalda Laranjo ◽  
Carlos Miguel Marto ◽  
Ana Margarida Abrantes ◽  
João Casalta-Lopes ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Cellular Response ◽  
Colorimetric Assay ◽  
Mineral Trioxide Aggregate ◽  
Mouse Cell ◽  
Direct Pulp Capping ◽  
Alizarin Red ◽  
Pulp Capping ◽  
Dentin Sialoprotein ◽  
And Function

(1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life®), mineral trioxide aggregate (WhiteProRoot®MTA) and calcium silicate (Biodentine™), in an odontoblast-like mouse cell line (MDPC-23). (2) Methods: Metabolic activity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT)assay, viability by the sulforhodamine B (SRB) assay, and the type of death and cell cycle analysis by flow cytometry. Alkaline phosphatase was evaluated by polymerase chain reaction (PCR), and dentin sialoprotein expression was assessed by immunocytochemistry. Mineralization was determined by the Alizarin Red S colorimetric assay and quantified by spectrophotometry. (3) Results: Life® induced a decrease in metabolic activity and viability, which is associated with an increase cell death. WhiteProRoot®MTA and Biodentine™ induced similar effects in cytotoxicity assays, with an increase in the expression of dentin sialoprotein (DSP) and formation of mineralized deposits, especially with Biodentine™. (4) Conclusions: The results of WhiteProRoot®MTA confirm its indication for these therapies, justifying its recognition as the “gold standard”. Biodentine™ may be an alternative, since they promote the same cellular response that mineral trioxide aggregate (MTA) does.

scholarly journals Temperature changes in the pulp chamber induced by polymerization of resin-based dental restoratives following simulated direct pulp capping

2019 ◽  
Vol 73 (4) ◽  
pp. 239-248
Author(s):  
Violeta Petrovic ◽  
Jovana Stasic ◽  
Vojislav Komlenic ◽  
Tatjana Savic-Stankovic ◽  
Marina Latkovic ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Mineral Trioxide Aggregate ◽  
Glass Ionomer ◽  
Direct Pulp Capping ◽  
Pulp Chamber ◽  
Temperature Changes ◽  
Pulp Capping ◽  
Dental Restoratives ◽  
Resin Modified Glass Ionomer ◽  
Light Curing

The objective of this study was to measure temperature changes in the pulp chamber induced by polymerization of resin-based dental restoratives following a simulated procedure of direct pulp capping. Class I cavities with a microperforation at the pulp horn were prepared in extracted human molar teeth. The complete procedure of direct pulp capping and cavity restoration was performed with the root part of extracted teeth fixed in a water bath at 37 ?C. Mineral trioxide aggregate, bioactive dentin substitute or calcium-hydroxide paste were used as pulp capping materials. Cavities were restored with a light-cured or chemically-cured resin-modified glass ionomer, universal adhesive and a bulk-fill composite, cured with a high-intensity LED unit. Pulp capping materials caused a slight temperature decrease. Lower temperature increase was recorded during light-curing of the glass ionomer liner after direct capping with mineral trioxide aggregate and calcium-hydroxide than that recorded for the bioactive dentin substitute. Adhesive light-curing increased temperature in all groups with higher mean temperatures in groups with chemically-cured as compared to those for the light-cured glass ionomer liner. Direct pulp capping with mineral trioxide aggregate or calcium-hydroxide followed by the light-cured resin-modified glass ionomer liner and a bonded bulk-fill composite restoration induced temperature changes below the potentially adverse threshold of 42.5?C.

Decision-Making and Management of Immature Permanent Teeth with Crown Fractures in Small Animals—A Review

2021 ◽  
pp. 089875642110463
Author(s):  
Amalia Zacher ◽  
Sandra Manfra Marretta
Keyword(s):  
Treatment Options ◽  
Mineral Trioxide Aggregate ◽  
Permanent Teeth ◽  
Small Animals ◽  
Unique Challenge ◽  
Pulp Capping ◽  
Pulp Therapy ◽  
Vital Pulp Therapy ◽  
Pulp Vitality ◽  
And Function

Immature permanent teeth with crown fractures present a unique challenge in human and animal patients. Immature permanent teeth have not yet developed completely, often presenting with thin dentin walls, incomplete apical formation, and increased crown-to-root ratios. Loss of pulp function at this stage has devastating long-term implications for these teeth. Ideally, attempts should be made to preserve pulp vitality in immature permanent teeth to allow for continued dental development. The range of treatment options for vital teeth includes odontoplasty with bonding and sealing +/− restoration, indirect pulp capping, and direct pulp capping/vital pulp therapy. These treatments have long been established in human and veterinary medicine, and cases have been reported in dogs and cats. Apexification using calcium hydroxide is a well-established treatment for nonvital immature teeth. The advent of mineral trioxide aggregate and other bioceramic materials for use in vital pulp therapy and apexification has reduced treatment sessions and improved outcomes. Recent developments in the field of regenerative endodontic therapy further expand treatment options and provide the possibility for continued development of a formerly nonvital tooth. Selecting the appropriate treatment based on the severity of tooth fracture and status of pulp vitality can avoid a lifetime of poor structure and function for the affected tooth. This article provides multiple step-by-step protocols for the management of immature permanent teeth with crown fractures in small animals.

scholarly journals Reparative Mineralized Tissue Characterization after Direct Pulp Capping with Calcium-Silicate-Based Cements

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2102 ◽  
Author(s):  
Xuan Tran ◽  
Hamideh Salehi ◽  
Minh Truong ◽  
Minic Sandra ◽  
Jeremy Sadoine ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Calcium Silicate ◽  
Tissue Characterization ◽  
Mineral Trioxide Aggregate ◽  
Clinical Situation ◽  
Mineralized Tissue ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Inflammatory Status ◽  
Reparative Dentin

Nowadays, the preservation of dental pulp vitality is an integral part of our daily therapies. The success of these treatments depends on the clinical situation as well as the biomaterials used. Mineral Trioxide aggregate and BiodentineTM are commonly used as pulp capping materials. One objective of vital pulp therapy is the repair/regeneration of the pulp. In addition to the initial inflammatory status of the pulp, the nature and quality of the new mineralized tissue obtained after pulp capping directly influence the success of the treatment. In order to characterize the reparative dentin, in the current study, the chemical composition and microstructure of the dentin bridge after direct pulp capping using Biodentine™ and mineral trioxide aggregate (MTA) was studied by using Raman microspectroscopy and scanning electron microscopy, respectively. The results showed that the reparative dentin bridge observed in both groups presented dentin tubules and chemical composition similar to primary dentin. With the limitations of this study, the calcium-silicate-based cements used as pulp capping materials provide an optimal environment for pulp healing, resulting in a reparative dentin resembling on certain points of the primary dentin and the regeneration of the pulp.

Direct Pulp Capping with Mineral Trioxide Aggregate: An Immunohistologic Comparison with Calcium Hydroxide in Rodents

2010 ◽  
Vol 36 (5) ◽  
pp. 814-819 ◽  
Author(s):  
Till Dammaschke ◽  
Udo Stratmann ◽  
Philipp Wolff ◽  
Darius Sagheri ◽  
Edgar Schäfer
Keyword(s):  
Calcium Hydroxide ◽  
Mineral Trioxide Aggregate ◽  
Direct Pulp Capping ◽  
Pulp Capping

Direct Pulp Capping with Calcium Hydroxide, Mineral Trioxide Aggregate, and Biodentine in Permanent Young Teeth with Caries: A Randomized Clinical Trial

2017 ◽  
Vol 43 (11) ◽  
pp. 1776-1780 ◽  
Author(s):  
Claudia Brizuela ◽  
Andrea Ormeño ◽  
Carolina Cabrera ◽  
Roxana Cabezas ◽  
Carolina Inostroza Silva ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Calcium Hydroxide ◽  
Randomized Clinical Trial ◽  
Mineral Trioxide Aggregate ◽  
Direct Pulp Capping ◽  
Pulp Capping

Direct Pulp Capping With Mineral Trioxide Aggregate

2008 ◽  
Vol 139 (3) ◽  
pp. 305-315 ◽  
Author(s):  
George Bogen ◽  
Jay S. Kim ◽  
Leif K. Bakland
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Direct Pulp Capping ◽  
Pulp Capping

Clinical Outcomes for Teeth Treated with Electrospun Poly(ε-caprolactone) Fiber Meshes/Mineral Trioxide Aggregate Direct Pulp Capping

2015 ◽  
Vol 41 (5) ◽  
pp. 628-636 ◽  
Author(s):  
Li-Wan Lee ◽  
Sheng-Huang Hsiao ◽  
Wei-Chiang Hung ◽  
Yun-Ho Lin ◽  
Po-Yu Chen ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Mineral Trioxide Aggregate ◽  
Direct Pulp Capping ◽  
Pulp Capping
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