scholarly journals Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1131 ◽  
Author(s):  
Oana Marișca ◽  
Nicolae Leopold
Keyword(s):  
In Vitro Cytotoxicity ◽  
Synthetic Approach ◽  
Physico Chemical ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Potential Applications ◽  
Internalization Rate ◽  
Enhanced Raman Scattering ◽  
Further Development

Gold nanoparticles (AuNPs) are the groundwork of a large variety of applications in the biomedical field. Further development and a better understanding of this versatile platform will lead to an expansion of potential applications. In this study, we propose a facile synthesis of AuNPs using hydrogen peroxide as a reducing agent and collagen as a stabilizing agent. Our synthetic approach results in “raspberry”-like AuNPs with a mean diameter of 60 nm, as revealed by electron microscopy. The optical properties of the AuNPs were assessed by UV-Vis and surface-enhanced Raman scattering (SERS), and their stability and in vitro cytotoxicity were evaluated as well. HeLa cell viability values were only modestly affected compared to control, with the highest concentration tested displaying a 20% decrease in cellular viability. The dose-dependent cellular internalization in the 20–60 nM range indicate the highest internalization rate at 60 nM and uptake values as high as 35%. This result correlated well with the viability results. These type of anisotropic AuNPs are proposed for biomedical applications such as hyperthermia, contrast agents or imaging. Therefore, our findings offer a platform for potential biological applications such as sensing and imaging, due to their unique physico-chemical features.

scholarly journals Multiplexed Liquid Biopsy and Tumor Imaging Using Surface-Enhanced Raman Scattering

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 449
Author(s):  
Francesco Dell’Olio
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Liquid Biopsy ◽  
Tumor Imaging ◽  
Surface Enhanced Raman Scattering ◽  
Detection Sensitivity ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

The recent improvements in diagnosis enabled by advances in liquid biopsy and oncological imaging significantly better cancer care. Both these complementary approaches, which are used for early tumor detection, characterization, and monitoring, can benefit from applying techniques based on surface-enhanced Raman scattering (SERS). With a detection sensitivity at the single-molecule level, SERS spectroscopy is widely used in cell and molecular biology, and its capability for the in vitro detection of several types of cancer biomarkers is well established. In the last few years, several intriguing SERS applications have emerged, including in vivo imaging for tumor targeting and the monitoring of drug release. In this paper, selected recent developments and trends in SERS applications in the field of liquid biopsy and tumor imaging are critically reviewed, with a special emphasis on results that demonstrate the clinical utility of SERS.

scholarly journals Optical Diagnostic Based on Functionalized Gold Nanoparticles

2019 ◽  
Vol 20 (18) ◽  
pp. 4346 ◽  
Author(s):  
Jiemei Ou ◽  
Zidan Zhou ◽  
Zhong Chen ◽  
Huijun Tan
Keyword(s):  
Optical Properties ◽  
Future Perspective ◽  
Au Nps ◽  
Optical Diagnostic ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Diagnostic Applications

Au nanoparticles (NPs) possess unique physicochemical and optical properties, showing great potential in biomedical applications. Diagnostic spectroscopy utilizing varied Au NPs has become a precision tool of in vitro and in vivo diagnostic for cancer and other specific diseases. In this review, we tried to comprehensively introduce the remarkable optical properties of Au NPs, including localized surfaces plasmon resonance (LSPR), surface-enhanced Raman scattering (SERS), and metal-enhanced fluorescence (MEF). Then, we highlighted the excellent works using Au NPs for optical diagnostic applications. Ultimately, the challenges and future perspective of using Au NPs for optical diagnostic were discussed.

scholarly journals Manipulation and Applications of Hotspots in Nanostructured Surfaces and Thin Films

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1667 ◽  
Author(s):  
Xiaoyu Zhao ◽  
Jiahong Wen ◽  
Aonan Zhu ◽  
Mingyu Cheng ◽  
Qi Zhu ◽  
...  
Keyword(s):  
Thin Films ◽  
Physical Vapor Deposition ◽  
Molecular Spectroscopy ◽  
Deposition Process ◽  
Etching Time ◽  
Nanostructured Surfaces ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Potential Applications ◽  
Enhanced Raman Scattering

The synthesis of nanostructured surfaces and thin films has potential applications in the field of plasmonics, including plasmon sensors, plasmon-enhanced molecular spectroscopy (PEMS), plasmon-mediated chemical reactions (PMCRs), and so on. In this article, we review various nanostructured surfaces and thin films obtained by the combination of nanosphere lithography (NSL) and physical vapor deposition. Plasmonic nanostructured surfaces and thin films can be fabricated by controlling the deposition process, etching time, transfer, fabrication routes, and their combination steps, which manipulate the formation, distribution, and evolution of hotspots. Based on these hotspots, PEMS and PMCRs can be achieved. This is especially significant for the early diagnosis of hepatocellular carcinoma (HCC) based on surface-enhanced Raman scattering (SERS) and controlling the growth locations of Ag nanoparticles (AgNPs) in nanostructured surfaces and thin films, which is expected to enhance the optical and sensing performance.

Surface enhanced Raman scattering based reaction monitoring of in vitro decyclization of creatinine → creatine

RSC Advances ◽  
2016 ◽  
Vol 6 (64) ◽  
pp. 58943-58949 ◽  
Author(s):  
Debraj Gangopadhyay ◽  
Poornima Sharma ◽  
Sachin Kumar Singh ◽  
Pushkar Singh ◽  
Volker Deckert ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Reaction Monitoring ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Raman signatures of decyclization of creatinine to creatine appear after 120 min at pH 8, 60 min at pH 10 and 30 min at pH 12. Signature of reversibility at later stages of the reaction.

In vitro concentration dependent detection of creatinine: a surface enhanced Raman scattering and fluorescence study

RSC Advances ◽  
2016 ◽  
Vol 6 (113) ◽  
pp. 112562-112567 ◽  
Author(s):  
Debraj Gangopadhyay ◽  
Poornima Sharma ◽  
Rajib Nandi ◽  
Moumita Das ◽  
Surajit Ghosh ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Fluorescence Spectra ◽  
Surface Enhanced Raman Scattering ◽  
Fluorescence Study ◽  
Consistent Variation ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

(a) SERS spectra and (b) fluorescence spectra of Jaffe complex showing consistent variation relatable to the concentration of CRN.

In vitro monitoring of ring opening of leflunomide: A surface enhanced Raman scattering and DFT based approach

2014 ◽  
Vol 613 ◽  
pp. 127-132 ◽  
Author(s):  
Poornima Sharma ◽  
Debraj Gangopadhyay ◽  
Pushkar Singh ◽  
P.C. Mishra ◽  
Volker Deckert ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Ring Opening ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

scholarly journals Common Aspects Influencing the Translocation of SERS to Biomedicine

2018 ◽  
Vol 25 (35) ◽  
pp. 4638-4652 ◽  
Author(s):  
Dionysia Tsoutsi ◽  
Marcos Sanles-Sobrido ◽  
Andreu Cabot ◽  
Pilar-Rivera Gil
Keyword(s):  
Raman Scattering ◽  
State Of The Art ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Diagnostic Technologies ◽  
The Impact

This review overviews the impact in biomedicine of surface enhanced. Raman scattering motivated by the great potential we believe this technique has. We present the advantages and limitations of this technique relevant to bioanalysis in vitro and in vivo and how this technique goes beyond the state of the art of traditional analytical, labelling and healthcare diagnostic technologies.

In vitro study of a pH-sensitive multifunctional doxorubicin–gold nanoparticle system: therapeutic effect and surface enhanced Raman scattering

RSC Advances ◽  
2015 ◽  
Vol 5 (81) ◽  
pp. 65651-65659 ◽  
Author(s):  
Kate Y. J. Lee ◽  
Yiqing Wang ◽  
Shuming Nie
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticle ◽  
In Vitro Study ◽  
Ph Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Nanoparticle System ◽  
Enhanced Raman Scattering ◽  
Nanoparticle Drug Delivery

A pH-sensitive multifunctional doxorubicin–gold nanoparticle drug delivery system, which has the potential to detect and treat tumors, was developed.

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