scholarly journals Intracellular Delivery of Biologically-Active Fungal Metabolite Gliotoxin Using Magnetic Nanoparticles

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1092
Author(s):  
Laura Comas ◽  
Esther Polo ◽  
M Pilar Domingo ◽  
Yulán Hernández ◽  
Maykel Arias ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Cancer Cells ◽  
Intracellular Delivery ◽  
Biologically Active ◽  
Therapeutic Drug ◽  
Fungal Metabolite

Keywords: magnetic nanoparticles; gliotoxin; therapeutic; drug delivery; cancer cells

Pseudo-branched polyester copolymer: an efficient drug delivery system to treat cancer

2020 ◽  
Vol 8 (6) ◽  
pp. 1592-1603
Author(s):  
Zachary Shaw ◽  
Arth Patel ◽  
Thai Butcher ◽  
Tuhina Banerjee ◽  
Ren Bean ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cancer Cells ◽  
Drug Delivery System ◽  
Delivery System ◽  
Diagnosis And Treatment ◽  
Therapeutic Drug

New aliphatic pseudo-branched polyester copolymers are synthesized from diethylmalonate. The formulated nanomedicine successfully encapsulates therapeutic drug in higher dosage and deliver specifically to cancer cells for diagnosis and treatment.

Synthesis of silver nanoparticles for the dual delivery of doxorubicin and alendronate to cancer cells

2015 ◽  
Vol 3 (36) ◽  
pp. 7237-7245 ◽  
Author(s):  
F. Benyettou ◽  
R. Rezgui ◽  
F. Ravaux ◽  
T. Jaber ◽  
K. Blumer ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Drug Delivery System ◽  
Delivery System ◽  
Silver Nanoparticle ◽  
Intracellular Delivery

We present the synthesis of a silver nanoparticle (AgNP) based drug-delivery system that achieves the simultaneous intracellular delivery of doxorubicin (Dox) and alendronate (Ald) and improves the anticancer therapeutic indices of both drugs.

scholarly journals Magnetoliposomes with size controllable insertion of magnetic nanoparticles for efficient targeting of cancer cells

RSC Advances ◽  
2019 ◽  
Vol 9 (26) ◽  
pp. 15053-15060
Author(s):  
Won Il Choi ◽  
Abhishek Sahu ◽  
Frederik R. Wurm ◽  
Seong-Min Jo
Keyword(s):  
Magnetic Nanoparticles ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Intracellular Delivery ◽  
Specific Targeting

Cationic magnetoliposomes enable to achieve efficient capture of cells and intracellular delivery of oligonucleotides into nucleus through cancer cell-specific targeting.

scholarly journals A Novel Method of Magnetic Nanoparticles Functionalized with Anti-Folate Receptor Antibody and Methotrexate for Antibody Mediated Targeted Drug Delivery

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 261
Author(s):  
Madeeha Shahzad Lodhi ◽  
Fatima Khalid ◽  
Muhammad Tahir Khan ◽  
Zahoor Qadir Samra ◽  
Shabbir Muhammad ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Cancer Cells ◽  
Hela Cells ◽  
Targeted Drug Delivery ◽  
Folate Receptor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Folate Receptors ◽  
Receptor Antibody ◽  
Targeted Drug

Therapeutic effects of anticancer medicines can be improved by targeting the specific receptors on cancer cells. Folate receptor (FR) targeting with antibody (Ab) is an effective tool to deliver anticancer drugs to the cancer cell. In this research project, a novel formulation of targeting drug delivery was designed, and its anticancer effects were analyzed. Folic acid-conjugated magnetic nanoparticles (MNPs) were used for the purification of folate receptors through a novel magnetic affinity purification method. Antibodies against the folate receptors and methotrexate (MTX) were developed and characterized with enzyme-linked immunosorbent assay and Western blot. Targeting nanomedicines (MNP-MTX-FR Ab) were synthesized by engineering the MNP with methotrexate and anti-folate receptor antibody (anti-FR Ab). The cytotoxicity of nanomedicines on HeLa cells was analyzed by calculating the % age cell viability. A fluorescent study was performed with HeLa cells and tumor tissue sections to analyze the binding efficacy and intracellular tracking of synthesized nanomedicines. MNP-MTX-FR Ab demonstrated good cytotoxicity along all the nanocomposites, which confirms that the antibody-coated medicine possesses the potential affinity to destroy cancer cells in the targeted drug delivery process. Immunohistochemical approaches and fluorescent study further confirmed their uptake by FRs on the tumor cells’ surface in antibody-mediated endocytosis. The current approach is a useful addition to targeted drug delivery for better management of cancer therapy along with immunotherapy in the future.

scholarly journals Doxorubicin–Gelatin/Fe3O4–Alginate Dual-Layer Magnetic Nanoparticles as Targeted Anticancer Drug Delivery Vehicles

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1747
Author(s):  
Chiung-Hua Huang ◽  
Ting-Ju Chuang ◽  
Cherng-Jyh Ke ◽  
Chun-Hsu Yao
Keyword(s):  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Drug Release ◽  
Cancer Cells ◽  
Anticancer Drug ◽  
Magnetic Layer ◽  
Drug Delivery Vehicles ◽  
Anticancer Drug Delivery ◽  
Delivery Vehicles ◽  
Mcf 7

In this study, magnetic nanoparticles composed of a core (doxorubicin–gelatin) and a shell layer (Fe3O4–alginate) were developed to function as targeted anticancer drug delivery vehicles. The anticancer drug doxorubicin (DOX) was selected as a model drug and embedded in the inner gelatin core to obtain high encapsulation efficiency. The advantage of the outer magnetic layer is that it targets the drug to the tumor tissue and provides controlled drug release. The physicochemical properties of doxorubicin–gelatin/Fe3O4–alginate nanoparticles (DG/FA NPs) were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction. The mean diameter of DG/FA NPs, which was determined using a zeta potential analyzer, was 401.8 ± 3.6 nm. The encapsulation rate was 64.6 ± 11.8%. In vitro drug release and accumulation were also studied. It was found that the release of DOX accelerated in an acidic condition. With the manipulation of an external magnetic field, DG/FA NPs efficiently targeted Michigan Cancer Foundation-7 (MCF-7) breast cancer cells and showed in the nucleus after 6 h of incubation. After 12 h of incubation, the relative fluorescence intensity reached 98.4%, and the cell viability of MCF-7 cells decreased to 52.3 ± 4.64%. Dual-layer DG/FA NPs could efficiently encapsulate and deliver DOX into MCF-7 cells to cause the death of cancer cells. The results show that DG/FA NPs have the potential for use in targeted drug delivery and cancer therapy.

scholarly journals Enhanced antitumor efficacy on colon cancer using EGF functionalized PLGA nanoparticles loaded with 5-Fluorouracil and perfluorocarbon

2019 ◽  
Author(s):  
pingping Wu ◽  
Qing Zhou ◽  
Huayun Zhu ◽  
Yan Zhuang ◽  
Jun Bao
Keyword(s):  
Drug Delivery ◽  
Colon Cancer ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Drug Delivery System ◽  
Delivery System ◽  
Plga Nanoparticles ◽  
Therapeutic Drug ◽  
Histopathological Analysis ◽  
Colon Cancer Cells

Abstract Background: Recurrence and metastasis are the shortcomings of the clinical treatment of colon cancer. Finding an efficacy strategy for the treatment of colon cancer is important. In recent years, poly lactic-co-glycolic acid (PLGA) has been shown to have potential as a broad therapeutic drug delivery system. This study aimed to design a dual-loaded nanoparticles drug delivery system to overcome the limitations of chemotherapeutic drugs in colon cancer therapy. Methods: We developed epidermal growth factor (EGF) functionalized poly PLGA nanoparticles (NPs) co-loaded with 5-fluorouracil (5Fu) and perfluorocarbon (PFC) (EGF-PLGA@5Fu/PFC NPs) for target therapy of colon cancer. EGF-PLGA@5Fu /PFC NPs were estimated by morphology, size distribution, in vitro stability and release profile. CCK-8, Hoechst33342 staining and flow cytometry assays were performed to investigate the functions of EGF-PLGA@5Fu/PFC NPs in SW620 cells. Results: We found that EGF-PLGA@5Fu/PFC NPs had an average size of 200 nm with a 5Fu-loading efficiency of 7.29%. Targeted EGF-PLGA@5Fu/PFC NPs exhibited higher cellular uptake than non-targeted NPs in colon cancer cells. EGF-PLGA@5Fu/PFC NPs were found to have the best efficiency on cell viability suppression and apoptosis induction in SW620 colon cancer cells. In xenograft mice, EGF-PLGA@5Fu/PFC NPs had the best suppressive effects on tumor growth compared with 5Fu, PLGA@5Fu and PLGA@5Fu/PFC NPs. The results of histopathological analysis further indicated that EGF-targeted NPs were the most efficient on tumor growth inhibition. Mechanically, the data demonstrated the improved therapeutic outcomes were owing to the fact that PFC relieved tumor hypoxia via transporting oxygen to the tumor. Conclusions: We creatively constructed a biocompatible nanodrug delivery system and functionalized nanoparticles might provide new potential for selective delivery of chemotherapy drugs to cancers.

scholarly journals Enhanced antitumor efficacy on colon cancer using EGF functionalized PLGA nanoparticles loaded with 5-Fluorouracil and perfluorocarbon

2020 ◽  
Author(s):  
Pingping Wu(Former Corresponding Author) ◽  
Qing Zhou ◽  
Huayun Zhu ◽  
Yan Zhuang ◽  
Jun Bao(New Corresponding Author)
Keyword(s):  
Drug Delivery ◽  
Colon Cancer ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Drug Delivery System ◽  
Delivery System ◽  
Plga Nanoparticles ◽  
Therapeutic Drug ◽  
Histopathological Analysis ◽  
Colon Cancer Cells

Abstract Background: Recurrence and metastasis are the shortcomings of the clinical treatment of colon cancer. Finding an efficacy strategy for the treatment of colon cancer is important. In recent years, poly lactic-co-glycolic acid (PLGA) has been shown to have potential as a broad therapeutic drug delivery system. This study aimed to design a dual-loaded nanoparticles drug delivery system to overcome the limitations of chemotherapeutic drugs in colon cancer therapy. Methods: We developed epidermal growth factor (EGF) functionalized poly PLGA nanoparticles (NPs) co-loaded with 5-fluorouracil (5Fu) and perfluorocarbon (PFC) (EGF-PLGA@5Fu/PFC NPs) for target therapy of colon cancer. EGF-PLGA@5Fu /PFC NPs were estimated by morphology, size distribution, in vitro stability and release profile. CCK-8, Hoechst33342 staining and flow cytometry assays were performed to investigate the functions of EGF-PLGA@5Fu/PFC NPs in SW620 cells. Results: We found that EGF-PLGA@5Fu/PFC NPs had an average size of 200 nm with a 5Fu-loading efficiency of 7.29%. Targeted EGF-PLGA@5Fu/PFC NPs exhibited higher cellular uptake than non-targeted NPs in colon cancer cells. EGF-PLGA@5Fu/PFC NPs were found to have the best efficiency on cell viability suppression and apoptosis induction in SW620 colon cancer cells. In xenograft mice, EGF-PLGA@5Fu/PFC NPs had the best suppressive effects on tumor growth compared with 5Fu, PLGA@5Fu and PLGA@5Fu/PFC NPs. The results of histopathological analysis further indicated that EGF-targeted NPs were the most efficient on tumor growth inhibition. Mechanically, the data demonstrated the improved therapeutic outcomes were owing to the fact that PFC relieved tumor hypoxia via transporting oxygen to the tumor. Conclusions: We creatively constructed a biocompatible nanodrug delivery system and functionalized nanoparticles might provide new potential for selective delivery of chemotherapy drugs to cancers.

The Improvement of Paclitaxel Cytotoxicity using Nanocellulose based Nature Resources

2019 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
R Nahrowi ◽  
A Setiawan ◽  
Noviany Noviany ◽  
I Sukmana ◽  
S D Yuwono
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Natural Resources ◽  
Cell Viability ◽  
Cancer Cells ◽  
Drug Delivery System ◽  
Target Cell ◽  
Mitotic Cycle ◽  
Potential Sources ◽  
Local Accumulation

Paclitaxel is one of the cancer drugs that often used. These drug kills cancer cells byinhibiting mitotic cycle. The efficiency of paclitaxel is increased by the use ofnanomaterials as a carrier of paclitaxel. Nanomaterials can enhance encapsulationefficiency, improve the drug release to the target cell following nanomaterialdegradation, and improve local accumulation of drug in the cell through endocytosisreceptor. Nanomaterial that often used forencapsulation of paclitaxel is a polymerderived from natural resources such as cellulose. The advantages of cellulose as acarrier of paclitaxel are nontoxic, biodegradable, and very abundant from varioussources. One of the potential sources of cellulose for drug delivery system is cassavabaggase.Keywords: Paclitaxel, encapsulation, cell viability, nanocellulose

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