scholarly journals Fibrin-Modified Cellulose as a Promising Dressing for Accelerated Wound Healing

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2314 ◽  
Author(s):  
Marketa Bacakova ◽  
Julia Pajorova ◽  
Tomas Sopuch ◽  
Lucie Bacakova
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Cell Attachment ◽  
Cellulose Fibers ◽  
Dermal Fibroblasts ◽  
Hydroxyl Groups ◽  
Structural Morphology

Dermal injuries and chronic wounds usually regenerate with scar formation. Successful treatment without scarring might be achieved by pre-seeding a wound dressing with cells. We aimed to prepare a wound dressing fabricated from sodium carboxymethylcellulose (Hcel® NaT), combined with fibrin and seeded with dermal fibroblasts in vitro. We fabricated the Hcel® NaT in a porous and homogeneous form (P form and H form, respectively) differing in structural morphology and in the degree of substitution of hydroxyl groups. Each form of Hcel® NaT was functionalized with two morphologically different fibrin structures to improve cell adhesion and proliferation, estimated by an MTS assay. Fibrin functionalization of the Hcel® NaT strongly enhanced colonization of the material with human dermal fibroblasts. Moreover, the type of fibrin structures influenced the ability of the cells to adhere to the material and proliferate on it. The fibrin mesh filling the void spaces between cellulose fibers better supported cell attachment and subsequent proliferation than the fibrin coating, which only enwrapped individual cellulose fibers. On the fibrin mesh, the cell proliferation activity on day 3 was higher on the H form than on the P form of Hcel® NaT, while on the fibrin coating, the cell proliferation on day 7 was higher on the P form. The Hcel® NaT wound dressing functionalized with fibrin, especially when in the form of a mesh, can accelerate wound healing by supporting fibroblast adhesion and proliferation.

scholarly journals Silk Sericin Semi-interpenetrating Network Hydrogels Based on PEG-Diacrylate for Wound Healing Treatment

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Patchara Punyamoonwongsa ◽  
Supattra Klayya ◽  
Warayuth Sajomsang ◽  
Chanikarn Kunyanee ◽  
Sasitorn Aueviriyavit
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Sol Gel ◽  
Aqueous Media ◽  
Gelation Time ◽  
Dermal Fibroblasts ◽  
Interpenetrating Network ◽  
Silk Sericin ◽  
The Future

Silk sericin (SS) from the Bombyx mori silk cocoons has received much attention from biomedical scientists due to its outstanding properties, such as antioxidant, antibacterial, UV-resistant, and ability to release moisturizing factors. Unmodified SS does not self-assemble strongly enough to be used as a hydrogel wound dressing. Therefore, there is a need for suitable stabilization techniques to interlink the SS peptide chains or strengthen their structural cohesion. Here, we reported a method to form a silk semi-interpenetrating network (semi-IPN) structure through reacting with the short-chain poly(ethylene glycol) diacrylate (PEGDA) in the presence of a redox pair. Various hydrogels were prepared in aqueous media at the final SS/PEGDA weight percentages of 8/92, 15/85, and 20/80. Results indicated that all semi-IPN samples underwent a sol-gel transition within 70 min. The equilibrium water content (EWC) for all samples was found to be in the range of 70-80%, depending on the PEGDA content. Both the gelation time and the sol fraction decreased with the increased PEGDA content. This was due to the tightened network structure formed within the hydrogel matrices. Among all hydrogel samples, the 15/85 (SS/PEGDA) hydrogel displayed the maximum compressive strength (0.66 MPa) and strain (7.15%), higher than those of pure PEGDA. This implied a well-balanced molecular interaction within the SS/PEGDA/water systems. Based on the direct and indirect MTS assay, the 15/85 hydrogel showed excellent in vitro biocompatibility towards human dermal fibroblasts, representing a promising material for biomedical wound dressing in the future. A formation of a semi-IPN structure has thus proved to be one of the best strategies to extend a practical limit of using SS hydrogels for wound healing treatment or other biomedical hydrogel matrices in the future.

scholarly journals Dermal fibroblasts cultured from donors with type 2 diabetes mellitus retain an epigenetic memory associated with poor wound healing responses

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aaiad H. A. Al-Rikabi ◽  
Desmond J. Tobin ◽  
Kirsten Riches-Suman ◽  
M. Julie Thornton
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Wound Healing ◽  
Type 2 Diabetes Mellitus ◽  
Chronic Wounds ◽  
Dermal Fibroblasts ◽  
Necrosis Factor Alpha ◽  
Tnf Α

AbstractThe prevalence of Type 2 diabetes mellitus (T2DM) is escalating globally. Patients suffer from multiple complications including the development of chronic wounds that can lead to amputation. These wounds are characterised by an inflammatory environment including elevated tumour necrosis factor alpha (TNF-α). Dermal fibroblasts (DF) are critical for effective wound healing, so we sought to establish whether there were any differences in DF cultured from T2DM donors or those without diabetes (ND-DF). ND- and T2DM-DF when cultured similarly in vitro secreted comparable concentrations of TNF-α. Functionally, pre-treatment with TNF-α reduced the proliferation of ND-DF and transiently altered ND-DF morphology; however, T2DM-DF were resistant to these TNF-α induced changes. In contrast, TNF-α inhibited ND- and T2DM-DF migration and matrix metalloprotease expression to the same degree, although T2DM-DF expressed significantly higher levels of tissue inhibitor of metalloproteases (TIMP)-2. Finally, TNF-α significantly increased the secretion of pro-inflammatory cytokines (including CCL2, CXCL1 and SERPINE1) in ND-DF, whilst this effect in T2DM-DF was blunted, presumably due to the tendency to higher baseline pro-inflammatory cytokine expression observed in this cell type. Collectively, these data demonstrate that T2DM-DF exhibit a selective loss of responsiveness to TNF-α, particularly regarding proliferative and secretory functions. This highlights important phenotypic changes in T2DM-DF that may explain the susceptibility to chronic wounds in these patients.

scholarly journals Dissolved oxygen from microalgae-gel patch promotes chronic wound healing in diabetes

2020 ◽  
Vol 6 (20) ◽  
pp. eaba4311 ◽  
Author(s):  
Huanhuan Chen ◽  
Yuhao Cheng ◽  
Jingrun Tian ◽  
Peizheng Yang ◽  
Xuerao Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Dissolved Oxygen ◽  
Oxygen Delivery ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Superior Performance ◽  
Gaseous Oxygen ◽  
Promote Cell Proliferation

Chronic wounds in diabetes undergo a lifetime risk of developing into diabetic foot ulcers. Oxygen is crucial to wound healing by regulating cell proliferation, migration, and neovascularization. However, current oxygen therapies, including hyperbaric oxygen (HBO) and topical gaseous oxygen (TGO), mainly employ gaseous oxygen delivery, which is much less effective in penetrating the skin. Here, we introduce an oxygen-producing patch, made of living microalgae hydrogel, which can produce dissolved oxygen. The superior performance of the patch that results from its dissolved oxygen delivery is >100-fold much more efficient than TGO penetrating the skin. Further experiments indicate that the patch could promote cell proliferation, migration, and tube formation in vitro, and improve chronic wound healing and the survival of skin grafts in diabetic mice. We believe that the microalgae-gel patch can provide continuous dissolved oxygen to improve chronic wound healing.

scholarly journals Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of Stellaria media (L.) Vill

2021 ◽  
Vol 11 (23) ◽  
pp. 11526
Author(s):  
Florina Miere (Groza) ◽  
Alin Cristian Teușdea ◽  
Vasile Laslo ◽  
Simona Cavalu ◽  
Luminița Fritea ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Antioxidant Capacity ◽  
Chronic Wounds ◽  
Dermal Fibroblasts ◽  
Skin Wounds ◽  
Stellaria Media ◽  
Herbal Products ◽  
Antioxidant Power

The healing of skin wounds remains an important concern in medicine, especially in chronic wounds caused by various diseases such as diabetes. Using herbs or herbal products to heal skin wounds is a therapeutic challenge for traditional medicine. In this context, the main aim of our work was to highlight the in vitro healing potential of Stellaria media (L.) Vill. (SM) extract using the scratch assay on normal human dermal fibroblasts (NHDF). The ability to stimulate cell migration and proliferation under the influence of different concentrations of SM extract (range between 12.5 and 200 µg/mL) was determined compared to the control (untreated in vitro-simulated wound) and positive control (allantoin 50 µg/mL). Our results showed that the concentration of 100 µg/mL SM extract applied on the simulated wound recorded the strongest and fastest (24 h) migration (with wound closure) and proliferation of NHDF compared with the control. In addition, the SM extract was characterized in terms of bioactive compounds (total phenols and flavonoids content), antioxidant capacity (FRAP (The Ferric-Reducing Antioxidant Power) assay and electrochemical method), and antimicrobial activity. The results show that the SM extract contains a considerable amount of polyphenols (17.19 ± 1.32 mg GAE/g dw and 7.28 ± 1.18 mg QE/g dw for total phenol and flavonoid content, respectively) with antioxidant capacity. Antimicrobial activity against Gram-positive bacteria (S. aureus) is higher than E. coli at a dose of 15 µg/mL. This study showed that Stellaria media is a source of polyphenols compounds with antioxidant capacity, and for the first time, its wound healing potential was emphasized.

scholarly journals Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies

Cells ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 917
Author(s):  
Rafael Ballesteros-Cillero ◽  
Evan Davison-Kotler ◽  
Nupur Kohli ◽  
William S. Marshall ◽  
Elena García-Gareta
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Chronic Wounds ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Surrounding Tissue ◽  
Cell Infiltration ◽  
Skin Substitutes ◽  
Vitro Model

Due to great clinical need, research where different biomaterials are tested as 3D scaffolds for skin tissue engineering has increased. In vitro studies use a cell suspension that is simply pipetted onto the material and cultured until the cells migrate and proliferate within the 3D scaffold, which does not mimic the in vivo reality. Our aim was to engineer a novel biomimetic in vitro model that mimics the natural cell infiltration process occurring in wound healing, thus offering a realistic approach when pre-screening and testing new skin substitutes. Our model consists of porous membrane cell culture inserts coated with gelatin and seeded with human dermal fibroblasts, inside which two different commercially available dermal substitutes were placed. Several features relevant to the wound healing process (matrix contraction, cell infiltration and proliferation, integration of the biomaterial with the surrounding tissue, and secretion of exogenous cytokines and growth factors) were evaluated. Our results showed that cells spontaneously infiltrate the materials and that our engineered model is able to induce and detect subtle differences between different biomaterials. The model allows for room for improvements or “adds-on” and miniaturization and can contribute to the development of functional and efficient skin substitutes for burns and chronic wounds.

scholarly journals Effect of Bioactive Phytochemicals from Phlomis viscosa Poiret on Wound Healing

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 609 ◽  
Author(s):  
Ludmila Yarmolinsky ◽  
Arie Budovsky ◽  
Leonid Yarmolinsky ◽  
Boris Khalfin ◽  
Vladimir Glukhman ◽  
...  
Keyword(s):  
Wound Healing ◽  
Beneficial Effect ◽  
Chronic Wounds ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
In Vivo Models ◽  
Bioactive Phytochemicals ◽  
Pro Inflammatory Cytokines

Phlomis viscosa Poiret is an evergreen shrub growing in Israel, Turkey, Lebanon, and Syria with acknowledged pro-wound healing (WH) properties. In this study, we evaluated the pro-WH potential of selected compounds found in this plant. Among the pro-WH compounds (identified by us) was a combination of three chemicals—diosmin, 1-octen-3-ol, and himachala-2,4-diene which enhanced WH significantly both in in vitro and in vivo models. The determined phytochemicals combination could be used for the treatment of chronic wounds. The effect of the extracts, diosmin, 1-octen-3-ol on the secretion of pro-inflammatory cytokines, IL-6 (A) and IL-8 (B) by human dermal fibroblasts was significant (p < 0.001). In addition, the beneficial effect of extracts of P. viscosa and its phytochemicals on WH was evidenced by inhibiting the growth of several WH delaying microorganisms.

scholarly journals Lupeol, a Pentacyclic Triterpene, Promotes Migration, Wound Closure, and Contractile Effect In Vitro: Possible Involvement of PI3K/Akt and p38/ERK/MAPK Pathways

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2819 ◽  
Author(s):  
Fernando Pereira Beserra ◽  
Meilang Xue ◽  
Gabriela Maia ◽  
Ariane Leite Rozza ◽  
Cláudia Helena Pellizzon ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Therapeutic Potential ◽  
Collagen Gel ◽  
Skin Wound ◽  
Dermal Fibroblasts ◽  
Pentacyclic Triterpene ◽  
Skin Wound Healing ◽  
Anti Inflammatory

Skin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 μg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.

Fabricated approach for an effective wound dressing material based on a natural gum impregnated with Acalypha indica extract

2018 ◽  
Vol 33 (6) ◽  
pp. 612-628 ◽  
Author(s):  
Preethi Jenifer ◽  
Mangathayaru Kalachaveedu ◽  
Arun Viswanathan ◽  
Arumugam Gnanamani ◽  
Mubeena
Keyword(s):  
Cell Proliferation ◽  
Polyvinyl Alcohol ◽  
Wound Dressing ◽  
Guar Gum ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
Raw Material ◽  
Cell Culture Study ◽  
Acalypha Indica

Although acute wounds are common, treatment for a scarless condition remains limited and ineffective as medicated dressings act only as an epidermal coverage and no interdermal interactions happen. This study examined the benefit of Acalypha indica, a traditionally acclaimed plant for wound healing, as a three-dimensional nanofibrous dressing. guar gum, a natural polysaccharide, was chosen as the raw material, in combination with a synthetic copolymer polyvinyl alcohol. A series of polymer blend nanofibers made of 3:7 of 1% wt guar and 10% wt polyvinyl alcohol along with varied ratios of A. indica were prepared using electrospinning. The effect of cross-linking by citric acid on the nanofibers was studied using Fourier transform infrared. A 5% wt A. indica content was optimized in the electrospun solution to get nanosized morphology, roughness, water absorbing capacity, thermal stability, and tensile strength. The composite material was found inhibitory to both Gram-positive and Gram-negative strains as measured by zone of inhibition. The ability of the dressing to support and proliferate human dermal fibroblasts was evaluated by cell proliferation assay and Calcein acetomethyl (AM) staining assay. The results confirmed that the composite dressing could support long-term cell growth in the 9-day cell culture study. The incorporation of A. indica to guar/polyvinyl alcohol composite nanofibers has thus resulted in a wound dressing material with nanosized morphology for mimicking the extracellular matrix, surface roughness for the absorbtion of proteins, swelling capacity for the absorption of exudates, antimicrobial activity for prevention of microbial infections, and cell proliferation activity for the complete wound closure. The electrospun guar/polyvinyl alcohol/ A. indica composite which proved to be a suitable dressing for acute wounds when ascertained in vitro can surely be developed as an ethical plant bioactive wound healant.

scholarly journals Fabrication of Hybrid Nanofibers from Biopolymers and Poly (Vinyl Alcohol)/Poly (ε-Caprolactone) for Wound Dressing Applications

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2104
Author(s):  
Sibusiso Alven ◽  
Blessing Atim Aderibigbe
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Vinyl Alcohol ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Cellular Adhesion ◽  
Wound Dressings ◽  
Poly Vinyl Alcohol ◽  
High Porosity ◽  
Hybrid Nanofibers

The management of chronic wounds is challenging. The factors that impede wound healing include malnutrition, diseases (such as diabetes, cancer), and bacterial infection. Most of the presently utilized wound dressing materials suffer from severe limitations, including poor antibacterial and mechanical properties. Wound dressings formulated from the combination of biopolymers and synthetic polymers (i.e., poly (vinyl alcohol) or poly (ε-caprolactone) display interesting properties, including good biocompatibility, improved biodegradation, good mechanical properties and antimicrobial effects, promote tissue regeneration, etc. Formulation of these wound dressings via electrospinning technique is cost-effective, useful for uniform and continuous nanofibers with controllable pore structure, high porosity, excellent swelling capacity, good gaseous exchange, excellent cellular adhesion, and show a good capability to provide moisture and warmth environment for the accelerated wound healing process. Based on the above-mentioned outstanding properties of nanofibers and the unique properties of hybrid wound dressings prepared from poly (vinyl alcohol) and poly (ε-caprolactone), this review reports the in vitro and in vivo outcomes of the reported hybrid nanofibers.

scholarly journals Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays

2021 ◽  
Vol 19 ◽  
pp. 228080002198969
Author(s):  
Min-Xia Zhang ◽  
Wan-Yi Zhao ◽  
Qing-Qing Fang ◽  
Xiao-Feng Wang ◽  
Chun-Ye Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Type I Collagen ◽  
Inhibition Zone ◽  
Negative Control ◽  
Type I ◽  
Nih3t3 Cells ◽  
Post Operation

The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by E. coli and S. aureus. Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.

Sign in / Sign up

Export Citation Format

Share Document