scholarly journals Accurate Calibration in Multi-Material 3D Bioprinting for Tissue Engineering

Materials ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 1402 ◽  
Author(s):  
Enrique Sodupe-Ortega ◽  
Andres Sanz-Garcia ◽  
Alpha Pernia-Espinoza ◽  
Carmen Escobedo-Lucea
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Calibration Method ◽  
3D Bioprinting ◽  
Vascular Networks ◽  
Single Session ◽  
Systems And Control ◽  
Complex Relationships ◽  
And Control ◽  
Printing Parameters

Most of the studies in three-dimensional (3D) bioprinting have been traditionally based on printing a single bioink. Addressing the complexity of organ and tissue engineering, however, will require combining multiple building and sacrificial biomaterials and several cells types in a single biofabrication session. This is a significant challenge, and, to tackle that, we must focus on the complex relationships between the printing parameters and the print resolution. In this paper, we study the influence of the main parameters driven multi-material 3D bioprinting and we present a method to calibrate these systems and control the print resolution accurately. Firstly, poloxamer hydrogels were extruded using a desktop 3D printer modified to incorporate four microextrusion-based bioprinting (MEBB) printheads. The printed hydrogels provided us the particular range of printing parameters (mainly printing pressure, deposition speed, and nozzle z-offset) to assure the correct calibration of the multi-material 3D bioprinter. Using the printheads, we demonstrated the excellent performance of the calibrated system extruding different fluorescent bioinks. Representative multi-material structures were printed in both poloxamer and cell-laden gelatin-alginate bioinks in a single session corroborating the capabilities of our system and the calibration method. Cell viability was not significantly affected by any of the changes proposed. We conclude that our proposal has enormous potential to help with advancing in the creation of complex 3D constructs and vascular networks for tissue engineering.

scholarly journals 3D Bioprinting of Vascularized Tissues for in vitro and in vivo Applications

2021 ◽  
Vol 9 ◽  
Author(s):  
Earnest P. Chen ◽  
Zeren Toksoy ◽  
Bruce A. Davis ◽  
John P. Geibel
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
Rapid Development ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Vascular Networks ◽  
Main Challenge ◽  
3D Printed

With a limited supply of organ donors and available organs for transplantation, the aim of tissue engineering with three-dimensional (3D) bioprinting technology is to construct fully functional and viable tissue and organ replacements for various clinical applications. 3D bioprinting allows for the customization of complex tissue architecture with numerous combinations of materials and printing methods to build different tissue types, and eventually fully functional replacement organs. The main challenge of maintaining 3D printed tissue viability is the inclusion of complex vascular networks for nutrient transport and waste disposal. Rapid development and discoveries in recent years have taken huge strides toward perfecting the incorporation of vascular networks in 3D printed tissue and organs. In this review, we will discuss the latest advancements in fabricating vascularized tissue and organs including novel strategies and materials, and their applications. Our discussion will begin with the exploration of printing vasculature, progress through the current statuses of bioprinting tissue/organoids from bone to muscles to organs, and conclude with relevant applications for in vitro models and drug testing. We will also explore and discuss the current limitations of vascularized tissue engineering and some of the promising future directions this technology may bring.

Hydrogel-Based Bioinks for Three-Dimensional Bioprinting: Patent Analysis

2021 ◽  
Vol 7 (1) ◽  
pp. 3
Author(s):  
Ahmed Fatimi
Keyword(s):  
Tissue Engineering ◽  
Detailed Analysis ◽  
State Of The Art ◽  
Three Dimensional ◽  
Patent Analysis ◽  
The State ◽  
Driving Factors ◽  
3D Bioprinting ◽  
Preparation Methods

There are a variety of hydrogel-based bioinks commonly used in three-dimensional bioprinting. In this study, in the form of patent analysis, the state of the art has been reviewed by introducing what has been patented in relation to hydrogel-based bioinks. Furthermore, a detailed analysis of the patentability of the used hydrogels, their preparation methods and their formulations, as well as the 3D bioprinting process using hydrogels, have been provided by determining publication years, jurisdictions, inventors, applicants, owners, and classifications. The classification of patents reveals that most inventions intended for hydrogels used as materials for prostheses or for coating prostheses are characterized by their function or properties Knowledge clusters and expert driving factors show that biomaterials, tissue engineering, and biofabrication research is concentrated in the most patents.

scholarly journals Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3522
Author(s):  
Su Jeong Lee ◽  
Jun Hee Lee ◽  
Jisun Park ◽  
Wan Doo Kim ◽  
Su A Park
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Research Groups ◽  
Porcine Skin ◽  
Alginate Hydrogel ◽  
Chemical Treatments ◽  
Soft Tissue Engineering

Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting.

The Fabrication of Tissue Engineering Scaffolds by Inkjet Printing Technology

2018 ◽  
Vol 934 ◽  
pp. 129-133 ◽  
Author(s):  
Chao Fan Lv ◽  
Li Ya Zhu ◽  
Jian Ping Shi ◽  
Zong An Li ◽  
Wen Lai Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Alginate ◽  
Inkjet Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Tissue Engineering Scaffolds ◽  
Printing Technology ◽  
3D Printed ◽  
Inkjet Printing Technology ◽  
Alginate Scaffold

Three-dimensional (3D) printing has been playing an important role in diverse areas in medicine. In order to promote the development of tissue engineering, this study attempts to fabricate tissue engineering scaffolds using the inkjet printing technology. Sodium alginate, exhibiting similar properties to the native human extracellular matrix (ECM), was used as bioink. The jetted fluid of sodium alginate would be gelatinized when printed into the calcium chloride solution. The characteristics of the 3D-printed sodium alginate scaffold were systematically measured and analyzed. The results show that, the pore size, porosity and degradation property of these scaffolds could be well controlled. This study indicates the capability of 3D bioprinting technology for preparing tissue engineering scaffolds.

scholarly journals Of balls, inks and cages: Hybrid biofabrication of 3D tissue analogs

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Nicanor Moldovan ◽  
Leni Maldovan ◽  
Michael Raghunath
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Hybrid Approach ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Support Structures ◽  
Cell Clusters ◽  
3D Space ◽  
Technical Solutions

The overarching principle of three-dimensional (3D) bioprinting is the placing of cells or cell clusters in the 3D space to generate a cohesive tissue microarchitecture that comes close to in vivo characteristics. To achieve this goal, several technical solutions are available, generating considerable combinatorial bandwidth: (i) Support structures are generated first, and cells are seeded subsequently; (ii) alternatively, cells are delivered in a printing medium, so-called “bioink,” that contains them during the printing process and ensures shape fidelity of the generated structure; and (iii) a “scaffold-free” version of bioprinting, where only cells are used and the extracellular matrix is produced by the cells themselves, also recently entered a phase of accelerated development and successful applications. However, the scaffold-free approaches may still benefit from secondary incorporation of scaffolding materials, thus expanding their versatility. Reversibly, the bioink-based bioprinting could also be improved by adopting some of the principles and practices of scaffold-free biofabrication. Collectively, we anticipate that combinations of these complementary methods in a “hybrid” approach, rather than their development in separate technological niches, will largely increase their efficiency and applicability in tissue engineering.

Bone Scaffold Fabrication System Study

2007 ◽  
Author(s):  
Lin Lu ◽  
David Wootton ◽  
Peter I. Lelkes ◽  
Jack Zhou
Keyword(s):  
Tissue Engineering ◽  
Flow Rate ◽  
Intelligent System ◽  
Bone Substitutes ◽  
Water Soluble ◽  
Health Concern ◽  
Bone Scaffold ◽  
Systems And Control ◽  
Porous Biomaterials ◽  
And Control

Musculoskeletal conditions are a major health concern in United States because of a large aging population and increased occurrence of sport-related injuries. The need for bone substitutes is especially important. Traditional treatments of bone-defect have many limitations. Bone tissue engineering may offer a less painful alternative to traditional bone grafts with lower risk of infection. This research integrates biomimetic modeling, solid freedom fabrication (SFF), systems and control, and tissue engineering in one intelligent system for structured, highly porous biomaterials, which will be applied to bone scaffolds. Recently a new SFF-based fabrication system has been developed, which uses a pressurized extrusion to print highly biocompatible and water soluble sucrose bone scaffold porogens. The fabrication process for PCL scaffold implemented and tested using the newly developed porogen system. The resultant scaffold demonstrates the defined porous structure designed into the sucrose porogens. The viscosity of sucrose mixture has been tested and analyzed. The flow rate measurements of sucrose machine have been carried out. The input factor, which induced uncertainty in the flow rate of the microprinting system has been analyzed. The result showed that the reservoir pressure was dominant to determine the flow rate. This is very important for improving the quality control of our fabrication system.

Nanomaterials for bioprinting: functionalization of tissue-specific bioinks

2021 ◽  
Author(s):  
Andrea S. Theus ◽  
Liqun Ning ◽  
Linqi Jin ◽  
Ryan K. Roeder ◽  
Jianyi Zhang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Biomedical Applications ◽  
Disease Modeling ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Significant Step ◽  
Printing Processes

Abstract Three-dimensional (3D) bioprinting is rapidly evolving, offering great potential for manufacturing functional tissue analogs for use in diverse biomedical applications, including regenerative medicine, drug delivery, and disease modeling. Biomaterials used as bioinks in printing processes must meet strict physiochemical and biomechanical requirements to ensure adequate printing fidelity, while closely mimicking the characteristics of the native tissue. To achieve this goal, nanomaterials are increasingly being investigated as a robust tool to functionalize bioink materials. In this review, we discuss the growing role of different nano-biomaterials in engineering functional bioinks for a variety of tissue engineering applications. The development and commercialization of these nanomaterial solutions for 3D bioprinting would be a significant step towards clinical translation of biofabrication.

Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device

2017 ◽  
Vol 9 (6) ◽  
pp. 506-518 ◽  
Author(s):  
Yuji Nashimoto ◽  
Tomoya Hayashi ◽  
Itsuki Kunita ◽  
Akiko Nakamasu ◽  
Yu-suke Torisawa ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Microfluidic Device ◽  
Three Dimensional ◽  
Vascular Networks

Creating vascular networks in tissues is crucial for tissue engineering.

scholarly journals Development of a Disposable Single-Nozzle Printhead for 3D Bioprinting of Continuous Multi-Material Constructs

Micromachines ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 459 ◽  
Author(s):  
Tiffany Cameron ◽  
Emad Naseri ◽  
Ben MacCallum ◽  
Ali Ahmadi
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Complex Geometries ◽  
3D Bioprinting ◽  
Mechanical Integrity ◽  
Silicon Coating

Fabricating multi-cell constructs in complex geometries is essential in the field of tissue engineering, and three-dimensional (3D) bioprinting is widely used for this purpose. To enhance the biological and mechanical integrity of the printed constructs, continuous single-nozzle printing is required. In this paper, a novel single-nozzle printhead for 3D bioprinting of multi-material constructs was developed and characterized. The single-nozzle multi-material bioprinting was achieved via a disposable, inexpensive, multi-fuse IV extension set; the printhead can print up to four different biomaterials. The transition distance of the developed printhead was characterized over a range of pressures and needle inner diameters. Finally, the transition distance was decreased by applying a silicon coating to the inner channels of the printhead.

scholarly journals 3D Bioprinting of Human Tissues: Biofabrication, Bioinks and Bioreactors

2021 ◽  
Vol 22 (8) ◽  
pp. 3971
Author(s):  
Jianhua Zhang ◽  
Esther Wehrle ◽  
Marina Rubert ◽  
Ralph Müller
Keyword(s):  
Tissue Engineering ◽  
Human Tissue ◽  
Fluid Shear Stress ◽  
Pharmaceutical Research ◽  
Three Dimensional ◽  
Biological Tissues ◽  
Layer By Layer ◽  
Human Tissues ◽  
3D Bioprinting

The field of tissue engineering has progressed tremendously over the past few decades in its ability to fabricate functional tissue substitutes for regenerative medicine and pharmaceutical research. Conventional scaffold-based approaches are limited in their capacity to produce constructs with the functionality and complexity of native tissue. Three-dimensional (3D) bioprinting offers exciting prospects for scaffolds fabrication, as it allows precise placement of cells, biochemical factors, and biomaterials in a layer-by-layer process. Compared with traditional scaffold fabrication approaches, 3D bioprinting is better to mimic the complex microstructures of biological tissues and accurately control the distribution of cells. Here, we describe recent technological advances in bio-fabrication focusing on 3D bioprinting processes for tissue engineering from data processing to bioprinting, mainly inkjet, laser, and extrusion-based technique. We then review the associated bioink formulation for 3D bioprinting of human tissues, including biomaterials, cells, and growth factors selection. The key bioink properties for successful bioprinting of human tissue were summarized. After bioprinting, the cells are generally devoid of any exposure to fluid mechanical cues, such as fluid shear stress, tension, and compression, which are crucial for tissue development and function in health and disease. The bioreactor can serve as a simulator to aid in the development of engineering human tissues from in vitro maturation of 3D cell-laden scaffolds. We then describe some of the most common bioreactors found in the engineering of several functional tissues, such as bone, cartilage, and cardiovascular applications. In the end, we conclude with a brief insight into present limitations and future developments on the application of 3D bioprinting and bioreactor systems for engineering human tissue.

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