scholarly journals Rough Titanium Oxide Coating Prepared by Micro-Arc Oxidation Causes Down-Regulation of hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor Jurkat T Cells

Materials ◽  
2018 ◽  
Vol 11 (3) ◽  
pp. 360 ◽  
Author(s):  
Igor Khlusov ◽  
Larisa Litvinova ◽  
Valeria Shupletsova ◽  
Olga Khaziakhmatova ◽  
Elena Melashchenko ◽  
...  
Keyword(s):  
T Cells ◽  
Titanium Oxide ◽  
Oxide Coating ◽  
Cytokine Secretion ◽  
Htert Expression ◽  
Jurkat T Cells ◽  
Down Regulation ◽  
Micro Arc Oxidation ◽  
Titanium Oxide Coating

scholarly journals Amorphous–Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3693
Author(s):  
Yurii P. Sharkeev ◽  
Ekaterina G. Komarova ◽  
Valentina V. Chebodaeva ◽  
Mariya B. Sedelnikova ◽  
Aleksandr M. Zakharenko ◽  
...  
Keyword(s):  
T Cells ◽  
Calcium Phosphate ◽  
Biological Effects ◽  
Electrical Potential ◽  
Biological Properties ◽  
Modern Trend ◽  
Calcium Phosphate Coating ◽  
Jurkat T Cells ◽  
Micro Arc Oxidation

A modern trend in traumatology, orthopedics, and implantology is the development of materials and coatings with an amorphous–crystalline structure that exhibits excellent biocopatibility. The structure and physico–chemical and biological properties of calcium phosphate (CaP) coatings deposited on Ti plates using the micro-arc oxidation (MAO) method under different voltages (200, 250, and 300 V) were studied. Amorphous, nanocrystalline, and microcrystalline statesof CaHPO4 and β-Ca2P2O7were observed in the coatings using TEM and XRD. The increase in MAO voltage resulted in augmentation of the surface roughness Ra from 2.5 to 6.5 µm, mass from 10 to 25 mg, thickness from 50 to 105 µm, and Ca/P ratio from 0.3 to 0.6. The electrical potential (EP) of the CaP coatings changed from −456 to −535 mV, while the zeta potential (ZP) decreased from −53 to −40 mV following an increase in the values of the MAO voltage. Numerous correlations of physical and chemical indices of CaP coatings were estimated. A decrease in the ZP magnitudes of CaP coatings deposited at 200–250 V was strongly associated with elevated hTERT expression in tumor-derived Jurkat T cells preliminarily activated with anti-CD2/CD3/CD28 antibodies and then contacted in vitro with CaP-coated samples for 14 days. In turn, in vitro survival of CD4+ subsets was enhanced, with proinflammatory cytokine secretion of activated Jurkat T cells. Thus, the applied MAO voltage allowed the regulation of the physicochemical properties of amorphous–crystalline CaP-coatings on Ti substrates to a certain extent. This method may be used as a technological mechanism to trigger the behavior of cells through contact with micro-arc CaP coatings. The possible role of negative ZP and Ca2+ as effectors of the biological effects of amorphous–crystalline CaP coatings is discussed. Micro-arc CaP coatings should be carefully tested to determine their suitability for use in patients with chronic lymphoid malignancies.

Influence of the Structure of the Titanium Oxide Coating Surface on Immunocompetent Tumor Cells

2016 ◽  
Vol 58 (11) ◽  
pp. 1527-1533 ◽  
Author(s):  
I. A. Khlusov ◽  
Yu. P. Sharkeev ◽  
V. F. Pichugin ◽  
E. V. Legostaeva ◽  
L. S. Litvinova ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Titanium Oxide ◽  
Oxide Coating ◽  
Coating Surface ◽  
Titanium Oxide Coating

Stoichiometry analysis of titanium oxide coating by LIBS

2009 ◽  
Vol 53 (1) ◽  
pp. 69-73 ◽  
Author(s):  
H. Estupiñán ◽  
D. Y. Peña ◽  
Y. O. García ◽  
R. Cabanzo ◽  
E. Mejía-Ospino
Keyword(s):  
Titanium Oxide ◽  
Oxide Coating ◽  
Titanium Oxide Coating

scholarly journals Galectin-8 Induces Apoptosis in Jurkat T Cells by Phosphatidic Acid-mediated ERK1/2 Activation Supported by Protein Kinase A Down-regulation

2009 ◽  
Vol 284 (19) ◽  
pp. 12670-12679 ◽  
Author(s):  
Andrés Norambuena ◽  
Claudia Metz ◽  
Lucas Vicuña ◽  
Antonia Silva ◽  
Evelyn Pardo ◽  
...  
Keyword(s):  
T Cells ◽  
Protein Kinase ◽  
Phosphatidic Acid ◽  
Protein Kinase A ◽  
Jurkat T Cells ◽  
Down Regulation ◽  
Kinase A

scholarly journals Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4307
Author(s):  
Larisa S. Litvinova ◽  
Olga G. Khaziakhmatova ◽  
Valeria V. Shupletsova ◽  
Kristina A. Yurova ◽  
Vladimir V. Malashchenko ◽  
...  
Keyword(s):  
T Cells ◽  
Calcium Phosphate ◽  
Tumor Cells ◽  
Surgical Stress ◽  
Microarc Oxidation ◽  
Calcium Phosphate Coating ◽  
Htert Expression ◽  
Cd4 Cells ◽  
Jurkat T Cells ◽  
X Ray

Calcium phosphate (CaP) materials are among the best bone graft substitutes, but their use in the repair of damaged bone in tumor patients is still unclear. The human Jurkat T lymphoblast leukemia-derived cell line (Jurkat T cells) was exposed in vitro to a titanium (Ti) substrate (10 × 10 × 1 mm3) with a bilateral rough (average roughness index (Ra) = 2–5 μm) CaP coating applied via the microarc oxidation (MAO) technique, and the morphofunctional response of the cells was studied. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscope (EDX) analyses showed voltage-dependent (150–300 V) growth of structural (Ra index, mass, and thickness) and morphological surface and volume elements, a low Ca/PaT ratio (0.3–0.6), and the appearance of crystalline phases of CaHPO4 (monetite) and β-Ca2P2O7 (calcium pyrophosphate). Cell and molecular reactions in 2-day and 14-day cultures differed strongly and correlated with the Ra values. There was significant upregulation of hTERT expression (1.7-fold), IL-17 secretion, the presentation of the activation antigens CD25 (by 2.7%) and CD95 (by 5.15%) on CD4+ cells, and 1.5–2-fold increased cell apoptosis and necrosis after two days of culture. Hyperactivation-dependent death of CD4+ cells triggered by the surface roughness of the CaP coating was proposed. Conversely, a 3.2-fold downregulation in hTERT expression increased the percentages of CD4+ cells and their CD95+ subset (by 15.5% and 22.9%, respectively) and inhibited the secretion of 17 of 27 test cytokines/chemokines without a reduction in Jurkat T cell survival after 14 days of coculture. Thereafter, cell hypoergy and the selection of an hTERT-independent viable CD4+ subset of tumor cells were proposed. The possible role of negative zeta potentials and Ca2+ as effectors of CaP roughness was discussed. The continuous (2–14 days) 1.5–6-fold reductions in the secretion of vascular endothelial growth factor (VEGF) by tumor cells correlated with the Ra values of microarc CaP-coated Ti substrates seems to limit surgical stress-induced metastasis of lymphoid malignancies.

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