scholarly journals Small but Smart: On the Diverse Role of Small Proteins in the Regulation of Cyanobacterial Metabolism

Life ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 322
Author(s):  
Fabian Brandenburg ◽  
Stephan Klähn
Keyword(s):  
Metabolic Pathways ◽  
Chemical Compounds ◽  
Industrial Processes ◽  
Biotechnological Applications ◽  
Major Focus ◽  
The Past ◽  
Metabolic Fluxes ◽  
Reactor Technology ◽  
Small Proteins

Over the past few decades, bioengineered cyanobacteria have become a major focus of research for the production of energy carriers and high value chemical compounds. Besides improvements in cultivation routines and reactor technology, the integral understanding of the regulation of metabolic fluxes is the key to designing production strains that are able to compete with established industrial processes. In cyanobacteria, many enzymes and metabolic pathways are regulated differently compared to other bacteria. For instance, while glutamine synthetase in proteobacteria is mainly regulated by covalent enzyme modifications, the same enzyme in cyanobacteria is controlled by the interaction with unique small proteins. Other prominent examples, such as the small protein CP12 which controls the Calvin–Benson cycle, indicate that the regulation of enzymes and/or pathways via the attachment of small proteins might be a widespread mechanism in cyanobacteria. Accordingly, this review highlights the diverse role of small proteins in the control of cyanobacterial metabolism, focusing on well-studied examples as well as those most recently described. Moreover, it will discuss their potential to implement metabolic engineering strategies in order to make cyanobacteria more definable for biotechnological applications.

Effective Rehabilitation Methods in Patients with Multiple Sclerosis

US Neurology ◽  
2010 ◽  
Vol 05 (02) ◽  
pp. 67 ◽  
Author(s):  
Susan E Bennett ◽  
Keyword(s):  
Multiple Sclerosis ◽  
Functional Recovery ◽  
Therapeutic Intervention ◽  
Aerobic Training ◽  
Major Focus ◽  
Pharmacological Management ◽  
Comprehensive Management ◽  
The Past ◽  
Moderate Disease

The role of rehabilitation in managing symptoms of multiple sclerosis (MS) and promoting functional recovery has grown exponentially over the past 12 years. Evidence now supports strength and aerobic training for individuals with mild or moderate disease. An emerging body of evidence suggests that exercise may play a role in the production of brain-derived neurotrophic factor in individuals with MS, and the combination of pharmacological management and rehabilitation may produce better outcomes than one therapeutic intervention alone. Rehabilitation and exercise should be a major focus in the comprehensive management of MS.

scholarly journals Engineering Microbial Consortia towards Bioremediation

Water ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 2928
Author(s):  
Xianglong Li ◽  
Shanghua Wu ◽  
Yuzhu Dong ◽  
Haonan Fan ◽  
Zhihui Bai ◽  
...  
Keyword(s):  
Social Interactions ◽  
Metabolic Pathways ◽  
Commercial Production ◽  
Microbial Consortia ◽  
The Past ◽  
Ecological Mechanisms ◽  
Toxic Constituent ◽  
Constituent Elements ◽  
Hazardous Compounds

Bioremediation is a sustainable remediation technology as it utilizes microorganisms to convert hazardous compounds into their less toxic or non-toxic constituent elements. This technology has achieved some success in the past decades; however, factors involving microbial consortia, such as microbial assembly, functional interactions, and the role of member species, hinder its development. Microbial consortia may be engineered to reconfigure metabolic pathways and reprogram social interactions to get the desired function, thereby providing solutions to its inherent problems. The engineering of microbial consortia is commonly applied for the commercial production of biomolecules. However, in the field of bioremediation, the engineering of microbial consortia needs to be emphasized. In this review, we will discuss the molecular and ecological mechanisms of engineering microbial consortia with a particular focus on metabolic cross-feeding within species and the transfer of metabolites. We also discuss the advantages and limitations of top-down and bottom-up approaches of engineering microbial consortia and their applications in bioremediation.

scholarly journals Flubromazolam overdose: A review of a new designer benzodiazepine and the role of flumazenil

2019 ◽  
Vol 9 (3) ◽  
pp. 133-137 ◽  
Author(s):  
Kristin Bohnenberger ◽  
Mei T. Liu
Keyword(s):  
Adverse Effects ◽  
Chemical Compounds ◽  
Quality Standards ◽  
The Internet ◽  
Online Forums ◽  
The Past ◽  
Pubmed Search ◽  
Designer Benzodiazepines

Abstract Designer products, a term referring to analogs of known chemical compounds with no established medical use, represent an easily accessible alternative to prescription-only products. During the past decade, designer benzodiazepines have become widely available on the online forums. Although these agents offer individuals an inexpensive and accessible alternative to prescription-only products, they are not without risk. Because of the lack of federally enforced quality standards, these designer products come with an intrinsic risk of unpredictable and potentially toxic adverse effects. This article presents a 36-year-old male with prolonged bradycardia resulting from the use of flubromazolam, a designer benzodiazepine purchased from the Internet. A PubMed search was conducted for flubromazolam, designer benzodiazepine, and flumazenil. This article will summarize the available literature regarding flubromazolam and the role of flumazenil in managing these overdoses.

scholarly journals Update on stress and depression: the role of the hypothalamic-pituitary-adrenal (HPA) axis

2003 ◽  
Vol 25 (4) ◽  
pp. 231-238 ◽  
Author(s):  
Andrea de Abreu Feijó de Mello ◽  
Marcelo Feijó de Mello ◽  
Linda L Carpenter ◽  
Lawrence H Price
Keyword(s):  
Hpa Axis ◽  
Life Stress ◽  
Early Life Stress ◽  
Major Focus ◽  
Hypothalamic Pituitary Adrenal ◽  
The Past ◽  
Hpa Axis Dysregulation ◽  
The Relationship ◽  
Adult Psychopathology

Over the past 50 years, relationships between stress and the neurobiological changes seen in psychiatric disorders have been well-documented. A major focus of investigations in this area has been the role of the hypothalamic-pituitary-adrenal (HPA) axis, both as a marker of stress response and as a mediator of additional downstream pathophysiologic changes. This review examines the emerging literature concerning the relationship between stress, HPA axis function, and depression, as well as the role of early life stress as an important risk factor for HPA axis dysregulation. The more recent studies reviewed suggest that the prominence of HPA axis hyperactivity in adults with depressive and anxiety disorders may constitute a link between the occurrence of adversity in childhood and the development of adult psychopathology

scholarly journals Modulation of Indian monsoon by water vapor and cloud feedback over the past 22,000 years

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Chetankumar Jalihal ◽  
Jayaraman Srinivasan ◽  
Arindam Chakraborty
Keyword(s):  
Water Vapor ◽  
Potential Model ◽  
Cloud Feedback ◽  
Climate Simulation ◽  
Major Focus ◽  
Solar Forcing ◽  
Internal Factors ◽  
The Past ◽  
Changing Role

AbstractTo predict how monsoons will evolve in the 21st century, we need to understand how they have changed in the past. In paleoclimate literature, the major focus has been on the role of solar forcing on monsoons but not on the amplification by feedbacks internal to the climate system. Here we have used the results from a transient climate simulation to show that feedbacks amplify the effect of change in insolation on the Indian summer monsoon. We show that during the deglacial (22 ka to 10 ka) monsoons were predominantly influenced by rising water vapor due to increasing sea surface temperature, whereas in the Holocene (10 ka to 0 ka) cloud feedback was more important. These results are consistent with another transient simulation, thus increasing confidence despite potential model biases. We have demonstrated that insolation drives monsoon through different pathways during cold and warm periods, thereby highlighting the changing role of internal factors.

On Future Directions in Pathology

1982 ◽  
Vol 40 ◽  
pp. 274-277
Author(s):  
Benjamin F. Trump ◽  
Irene K. Berezesky ◽  
Raymond T. Jones
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Clinical Pathology ◽  
Future Directions ◽  
Diagnostic Pathology ◽  
The Past ◽  
Transmission Electron ◽  
Organ Systems ◽  
The Many

The role of electron microscopy and associated techniques is assured in diagnostic pathology. At the present time, most of the progress has been made on tissues examined by transmission electron microscopy (TEM) and correlated with light microscopy (LM) and by cytochemistry using both plastic and paraffin-embedded materials. As mentioned elsewhere in this symposium, this has revolutionized many fields of pathology including diagnostic, anatomic and clinical pathology. It began with the kidney; however, it has now been extended to most other organ systems and to tumor diagnosis in general. The results of the past few years tend to indicate the future directions and needs of this expanding field. Now, in addition to routine EM, pathologists have access to the many newly developed methods and instruments mentioned below which should aid considerably not only in diagnostic pathology but in investigative pathology as well.

Fluorescent potentiometric dyes for membrane-potential imaging

1994 ◽  
Vol 52 ◽  
pp. 166-167
Author(s):  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Optical Recording ◽  
Biological Processes ◽  
Major Focus ◽  
The Past ◽  
Excitable Systems ◽  
Major Application ◽  
The Impact

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.

Challenges of Fear Conditioning Research in the Age of RDoC

2017 ◽  
Vol 225 (3) ◽  
pp. 189-199 ◽  
Author(s):  
Tina B. Lonsdorf ◽  
Jan Richter
Keyword(s):  
Fear Conditioning ◽  
Clinical Diagnosis ◽  
Research Domain Criteria ◽  
Major Focus ◽  
The Future ◽  
Definition Of ◽  
Methodological Considerations ◽  
Research Domain

Abstract. As the criticism of the definition of the phenotype (i.e., clinical diagnosis) represents the major focus of the Research Domain Criteria (RDoC) initiative, it is somewhat surprising that discussions have not yet focused more on specific conceptual and procedural considerations of the suggested RDoC constructs, sub-constructs, and associated paradigms. We argue that we need more precise thinking as well as a conceptual and methodological discussion of RDoC domains and constructs, their interrelationships as well as their experimental operationalization and nomenclature. The present work is intended to start such a debate using fear conditioning as an example. Thereby, we aim to provide thought-provoking impulses on the role of fear conditioning in the age of RDoC as well as conceptual and methodological considerations and suggestions to guide RDoC-based fear conditioning research in the future.

A literature review on firms' internationalisation through e-commerce

2019 ◽  
pp. 121-143
Author(s):  
Riccardo Resciniti ◽  
Federica De Vanna
Keyword(s):  
Systematic Review ◽  
Literature Review ◽  
International Business ◽  
Decision Process ◽  
Foreign Markets ◽  
New Markets ◽  
Integrative Framework ◽  
The Past ◽  
The Relationship

The rise of e-commerce has brought considerable changes to the relationship between firms and consumers, especially within international business. Hence, understanding the use of such means for entering foreign markets has become critical for companies. However, the research on this issue is new and so it is important to evaluate what has been studied in the past. In this study, we conduct a systematic review of e-commerce and internationalisation studies to explicate how firms use e-commerce to enter new markets and to export. The studies are classified by theories and methods used in the literature. Moreover, we draw upon the internationalisation decision process (antecedents-modalities-consequences) to propose an integrative framework for understanding the role of e-commerce in internationalisation

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