scholarly journals Assessment of Ecological Condition of Haplic Chernozem Calcic Contaminated with Petroleum Hydrocarbons during Application of Bioremediation Agents of Various Natures

Land ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 169
Author(s):  
Tatiana Minnikova ◽  
Sergey Kolesnikov ◽  
Tatiana Minkina ◽  
Saglara Mandzhieva
Keyword(s):  
Soil Respiration ◽  
Petroleum Hydrocarbons ◽  
Soil Bacteria ◽  
Petroleum Hydrocarbon ◽  
Reference Value ◽  
Ecological Condition ◽  
Hydrocarbon Contamination ◽  
Sodium Humate ◽  
Integrated Index ◽  
Biological State

Petroleum hydrocarbon contamination disrupts ecological and agricultural soil functions. For their restoration, bioremediation agents of various natures are used (nonorganic or organic fertilizers, bacterial preparations, adsorbing agents) featuring different remediation mechanisms (adsorption or biostimulation of petroleum hydrocarbon decomposition). The objective of this research is the assessment of the ecological condition of petroleum hydrocarbon-contaminated Haplic Chernozem Calcic after the application of bioremediation agents of various natures. The influence of glauconite, nitroammophos, sodium humate, the bacterial preparation “Baikal EM-1”, and biochar on the intensity of petroleum hydrocarbon decomposition and the ecological condition of Haplic Chernozem Calcic was analyzed. The ecological condition of Haplic Chernozem Calcic was assessed based on the residual content of petroleum hydrocarbons in soil and the following biological parameters: changes in the number of soil bacteria, activity of catalase and dehydrogenases, soil respiration (CO2 emission), germinating ability, lengths of roots and shoots, and integrated index of the biological state. The minimum concentrations of residual petroleum hydrocarbons in soil were observed after the use of biochar (44% from initial content) and glauconite (49%). The biological properties of soils were affected in different ways. Soil respiration was stimulated by 3-6-fold after adding nitroammophos. Indices for the intensity of the early growth and germination of radish in soil with glauconite, sodium humate, and biochar were increased by 37–125% (p < 0.01) compared with the reference value. After the application of biochar, sodium humate, and “Baikal EM-1”, the number of soil bacteria was 66–289% higher (p < 0.01) than the reference value. At the same time, the activities of catalase and dehydrogenases were inhibited by up to 35% in variants with bioremediation agents and petroleum hydrocarbons relative to the reference values. The maximum stimulation of the biological activity (as the integrated index of the biological state (IISB)) of Haplic Chernozem Calcic was observed after applying sodium humate and biochar, with 70 and 66% (p < 0.01) increases from the reference value, respectively. Considering the net cost of bioremediation agents, the maximum cost efficiency is achieved with “Baikal EM-1”, sodium humate, and biochar: 110, 527, and 847 USD·103/ha, respectively. After using Baikal EM-1”, sodium humate, and biochar, the ecological state of Haplic Chernozem Calcic was restored.

The Remedial Effect of the Decomposing Bacteria on Different Petroleum Hydrocarbon Contamination

2011 ◽  
Vol 414 ◽  
pp. 88-92
Author(s):  
Xiao Nan Sun ◽  
An Ping Liu ◽  
Wen Ting Sun ◽  
Shu Chang Jin
Keyword(s):  
Growth Curve ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Carbon Chain ◽  
Hydrocarbon Contamination ◽  
Short Chain ◽  
Petroleum Contamination ◽  
Rate Curve ◽  
Petroleum Hydrocarbon Contamination

Petroleum contamination has become one of the major soil contaminations. Aiming at petroleum hydrocarbon contamination, the multi-group opposite experiments is set; this paper use some petroleum hydrocarbon-decomposing bacteria to remedy the soil contaminated by different carbon chain petroleum hydrocarbons. Compare and study the remedial results, and study the growth of the bacteria in the decomposing process. The Study shows that the degradation rate of the bacteria to short-chain petroleum hydrocarbons is relatively high; Within 40 days without nutrient substance, degradation rate of bacteria to gasoline and diesel is 80%, degradation rate of bacteria to aromatics and lubricants is 50%, the trend of bacteria’s growth curve and the degradation rate curve of each component are approximate.

scholarly journals Phytoremediation of hydrocarbon-contaminated soils with emphasis on the effect of petroleum hydrocarbons on the growth of plant species

2009 ◽  
Vol 89 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Ravanbakhsh Shirdam ◽  
Ali Daryabeigi Zand ◽  
Gholamreza Nabi Bidhendi ◽  
Nasser Mehrdadi
Keyword(s):  
Plant Species ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Green Technology ◽  
Hydrocarbon Contamination ◽  
Total Petroleum Hydrocarbons ◽  
Control Treatment ◽  
Polluted Soils

To date, many developing countries such as Iran have almost completely abandoned the idea of decontaminating oil-polluted soils due to the high costs of conventional (physical/chemical) soil remediation methods. Phytoremediation is an emerging green technology that can become a promising solution to the problem of decontaminating hydrocarbon-polluted soils. Screening the capacity of native tolerant plant species to grow on aged, petroleum hydrocarbon-contaminated soils is a key factor for successful phytoremediation. This study investigated the effect of hydrocarbon pollution with an initial concentration of 40 000 ppm on growth characteristics of sorghum (Sorghum bicolor) and common flax (Linum usitatissumum). At the end of the experiment, soil samples in which plant species had grown well were analyzed for total petroleum hydrocarbons (TPHs) removal by GC-FID. Common flax was used for the first time in the history of phytoremediation of oil-contaminated soil. Both species showed promising remediation efficiency in highly contaminated soil; however, petroleum hydrocarbon contamination reduced the growth of the surveyed plants significantly. Sorghum and common flax reduced TPHs concentration by 9500 and 18500 mg kg‑1, respectively, compared with the control treatment.

Application of Oxygen-Releasing Material to Enhance In Situ Aerobic Bioremediation

2012 ◽  
Vol 430-432 ◽  
pp. 1401-1404 ◽  
Author(s):  
T.Y. Chen ◽  
C.M. Kao ◽  
H.Y. Chiou ◽  
Y.T. Yu ◽  
W.P. Sung
Keyword(s):  
Release Rate ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Field Application ◽  
Hydrocarbon Contamination ◽  
Maintenance Cost ◽  
Oxygen Release ◽  
Calcium Peroxide ◽  
Aerobic Bioremediation

Contamination of groundwater by petroleum-hydrocarbons is a widespread environmental problem. Generally in plumes of petroleum-hydrocarbon contamination, the dissolved oxygen (DO) demand imposed by biodegradation of organic contaminants exceeds the DO available creating anaerobic conditions within the plume core and mid-plume areas. The objectives of this bench-scale study were to (1) develop oxygen-releasing materials for continuous oxygen supplement, (2) determine the optimal components of the studied oxygen release material, and (3) evaluate the oxygen release rate and lifetime of this material. Moreover, the potential of using a passive oxygen release material to clean up aquifers contaminated by petroleum hydrocarbons was also studied. Bench experiments were conducted to design and identify the components of the oxygen-releasing materials. The mixtures of the oxygen release material were prepared by blending gypsum, calcium peroxide (CaO2), sand, and water together at a ratio of 1：0.5：0.14：0.75 by weight. Cement was used as a binder and regular medium filter sand was used to increase the permeability of the mixture. Calcium peroxide releases oxygen upon contact water (2CaO2 + 2H2O → O2 + 2Ca(OH) 2). The designed material with a density of 1.1 g/cm3 was made of 3.5-cm cube for the batch experiment. Results show that the oxygen release rate of the material is 0.025 mg/day/g. The oxygen release material is able to remain active in oxygen release for more than three months. With the application of this developed oxygen release material, the contaminated subsurface can remain an aerobic environment for subsequent aerobic bioremediation. For the future field application, the developed materials can be placed in remediation wells, trenches, horizontal wells, or barriers. Thus, the passive biobarrier system has advantages over conventional system including less maintenance, cost-effectiveness, no above-ground facilities, no groundwater pumping and reinjection, no air pollution problems, and groundwater remediation in situ. The proposed treatment system would be expected to provide a more cost-effective alternative to remediate petroleum-hydrocarbon contaminated aquifers. This technology can also be applied for other hazardous waste contaminated sites.

scholarly journals Potential Disease Hazards of Petrolium Hydrocarbons Contamination in Gafrarium Pectinatum (Linnaeus, 1758) From Egyptian Lake Temsah with Emphasis On Gafrarium DNA Parcoding

2021 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Dna Damage ◽  
Cytochrome C ◽  
Comet Assay ◽  
Cytochrome C Oxidase ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Disease Risk ◽  
Monitoring Program ◽  
Hydrocarbon Contamination ◽  
Risk Probability

Assessment of petroleum hydrocarbon (PHC) contamination was carried out in water, sediments and the bivalve Gafrarium pictinatum as a part of monitoring program work. DNA barcoding was applied on G. pictinatum using Cytochrome c oxidase subunit 1 (COX1); accession no. HQ703080.1. assessment of disease risk probability due to PHC contamination using comet assay in G. pectinatum gills was studied. Results revealed that the value of dissolved petroleum hydrocarbons was 44 mg/l in water of the investigated area, 365.5 mg/kg in sediment and 135.2 mg/kg and in G. pictinatum tissue. Phylogeny of the collected bivalves was confirmed as Gafrarium pictinatum using COX1 representative primers, PCR, sequencing and blast tool on the NCBI. TPH caused DNA damage in G. pictinatum, the value of the damage differed from location to another which may be attributed to the level of hydrocarbons pollution. This study provides a basis for studying hydrocarbon contamination in marine environment.

scholarly journals Petroleum Hydrocarbon Contamination in Terrestrial Ecosystems—Fate and Microbial Responses

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3400 ◽  
Author(s):  
Adam Truskewycz ◽  
Taylor D. Gundry ◽  
Leadin S. Khudur ◽  
Adam Kolobaric ◽  
Mohamed Taha ◽  
...  
Keyword(s):  
Natural Attenuation ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Terrestrial Ecosystems ◽  
Hydrocarbon Contamination ◽  
Microbial Consortia ◽  
Metagenomic Sequencing ◽  
Petroleum Hydrocarbon Contamination ◽  
The Impact

Petroleum hydrocarbons represent the most frequent environmental contaminant. The introduction of petroleum hydrocarbons into a pristine environment immediately changes the nature of that environment, resulting in reduced ecosystem functionality. Natural attenuation represents the single, most important biological process which removes petroleum hydrocarbons from the environment. It is a process where microorganisms present at the site degrade the organic contaminants without the input of external bioremediation enhancers (i.e., electron donors, electron acceptors, other microorganisms or nutrients). So successful is this natural attenuation process that in environmental biotechnology, bioremediation has developed steadily over the past 50 years based on this natural biodegradation process. Bioremediation is recognized as the most environmentally friendly remediation approach for the removal of petroleum hydrocarbons from an environment as it does not require intensive chemical, mechanical, and costly interventions. However, it is under-utilized as a commercial remediation strategy due to incomplete hydrocarbon catabolism and lengthy remediation times when compared with rival technologies. This review aims to describe the fate of petroleum hydrocarbons in the environment and discuss their interactions with abiotic and biotic components of the environment under both aerobic and anaerobic conditions. Furthermore, the mechanisms for dealing with petroleum hydrocarbon contamination in the environment will be examined. When petroleum hydrocarbons contaminate land, they start to interact with its surrounding, including physical (dispersion), physiochemical (evaporation, dissolution, sorption), chemical (photo-oxidation, auto-oxidation), and biological (plant and microbial catabolism of hydrocarbons) interactions. As microorganism (including bacteria and fungi) play an important role in the degradation of petroleum hydrocarbons, investigations into the microbial communities within contaminated soils is essential for any bioremediation project. This review highlights the fate of petroleum hydrocarbons in tertial environments, as well as the contributions of different microbial consortia for optimum petroleum hydrocarbon bioremediation potential. The impact of high-throughput metagenomic sequencing in determining the underlying degradation mechanisms is also discussed. This knowledge will aid the development of more efficient, cost-effective commercial bioremediation technologies.

scholarly journals Metagenomic Characteristics of Bacterial Response to Petroleum Hydrocarbon Contamination in Diverse Environments as Revealed by Functional Taxonomic Strategies

2016 ◽  
Author(s):  
Arghya Mukherjee ◽  
Bobby Chettri ◽  
James S. Langpoklakpam ◽  
Pijush Basak ◽  
Aravind Prasad ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
High Throughput Sequencing ◽  
Hydrocarbon Contamination ◽  
Bioinformatic Tools ◽  
Bacterial Response ◽  
Petroleum Hydrocarbon Contamination ◽  
Microbial Remediation ◽  
Recent Developments ◽  
Polluted Sites

AbstractMicrobial remediation of oil polluted habitats remains one of the foremost methods for restoration of petroleum hydrocarbon contaminated environments. The development of effective bioremediation strategies however, require an extensive understanding of the resident microbiome of these habitats. Recent developments such as high-throughput sequencing has greatly facilitated the advancement of microbial ecological studies in oil polluted habitats. However, effective interpretation of biological characteristics from these large datasets remains a considerable challenge. In this study, we have implemented recently developed bioinformatic tools for analyzing 65 publicly available 16S rRNA datasets from 12 diverse hydrocarbon polluted habitats to decipher metagenomic characteristics of bacterial communities of the same. We have comprehensively described phylogenetic and functional compositions of these habitats and additionally inferred a multitude of metagenomic features including 255 taxa and 414 functional modules which can be used as biomarkers for effective distinction between the 12 oil polluted sites. We have identified essential metabolic signatures and also showed that significantly over-represented taxa often contribute to either or both, hydrocarbon degradation and additional important functions. Our findings reveal significant differences between hydrocarbon contaminated sites and establishes the importance of endemic factors in addition to petroleum hydrocarbons as driving factors for sculpting hydrocarbon contaminated bacteriomes.

scholarly journals Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

2021 ◽  
Author(s):  
Emilio D’Ugo ◽  
Milena Bruno ◽  
Arghya Mukherjee ◽  
Dhrubajyoti Chattopadhyay ◽  
Roberto Giuseppetti ◽  
...  
Keyword(s):  
Oil Spill ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Oil Pollution ◽  
Primary Source ◽  
Hydrocarbon Contamination ◽  
Petroleum Hydrocarbon Contamination ◽  
Microbial Response ◽  
Pollution Event ◽  
Hydrocarbonoclastic Potential

AbstractMicrobiomes of freshwater basins intended for human use remain poorly studied, with very little known about the microbial response to in situ oil spills. Lake Pertusillo is an artificial freshwater reservoir in Basilicata, Italy, and serves as the primary source of drinking water for more than one and a half million people in the region. Notably, it is located in close proximity to one of the largest oil extraction plants in Europe. The lake suffered a major oil spill in 2017, where approximately 400 tons of crude oil spilled into the lake; importantly, the pollution event provided a rare opportunity to study how the lacustrine microbiome responds to petroleum hydrocarbon contamination. Water samples were collected from Lake Pertusillo 10 months prior to and 3 months after the accident. The presence of hydrocarbons was verified and the taxonomic and functional aspects of the lake microbiome were assessed. The analysis revealed specialized successional patterns of lake microbial communities that were potentially capable of degrading complex, recalcitrant hydrocarbons, including aromatic, chloroaromatic, nitroaromatic, and sulfur containing aromatic hydrocarbons. Our findings indicated that changes in the freshwater microbial community were associated with the oil pollution event, where microbial patterns identified in the lacustrine microbiome 3 months after the oil spill were representative of its hydrocarbonoclastic potential and may serve as effective proxies for lacustrine oil pollution.

scholarly journals Biodegradation Potentials of Aspergillus sydowii and Fusarium lichenicola on Total Petroleum Hydrocarbon in an Oilfield Wastewater in Rivers State

2020 ◽  
pp. 1-7
Author(s):  
Williams, Janet Olufunmilayo ◽  
Aleruchi Owhonka
Keyword(s):  
Mixed Culture ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbons ◽  
Aspergillus Sydowii ◽  
Significant Difference ◽  
Set Up ◽  
Rivers State

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l.  The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the control on days 7 and 21 at p≤0.05. The results therefore revealed actual and significant reduction of TPHs in the mixed culture. In addition, there was clearance of n-alkanes by the mixed culture. This suggests that fungi have great potentials in biodegradation of TPHs and in remediation of TPH contaminated environments.

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