scholarly journals Methylene Blue and Proflavine as Intraarterial Marker for Functional Perforazome—Comparative Study

2021 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Maria-Eliza Nedu ◽  
Mihaela Tertis ◽  
Cecilia Cristea ◽  
Alexandru Valentin Georgescu
Keyword(s):  
Methylene Blue ◽  
Inflammatory Reaction ◽  
Wistar Rats ◽  
Inflammatory Infiltrate ◽  
Perforator Flap ◽  
Sentinel Lymph Nodes ◽  
In Vivo Evaluation ◽  
Gram Positive Bacteria ◽  
Long Time

Methylene blue (MB) is both a dye and a medicine known and used for a long time including as lymphatic tracer in melanoma and breast cancer for revealing sentinel lymph nodes. Proflavine (PRO) is an acriflavine dye, used as bacteriostatic disinfectant against many gram-positive bacteria that was also successfully applied to evaluate morphopathological changes in tissues. This study was performed on a group of twenty-eight Wistar rats and had as its main objective the in vivo evaluation of the use of MB and PRO as perforator tracers. The two dyes proved to be effective functional perforasome tracers with medium inflammatory infiltrate in the skin of the island perforator flap which heals perfectly at 14 days with complete absence of the inflammatory reaction. At the same injected amount, PRO seems to determine a greater inflammatory reaction compared with MB, but in smaller concentration, the inflammatory response is absent in the case of PRO. In conclusion, both substances tested within this in vivo study are good functional perforasome tracers, but PRO has the advantage of the absence of inflammatory reaction when using lower concentrations, while preserving unalerted its efficiency as tracer.

Physiological and Biochemical Evaluation of the Effectiveness of a New Food Ingredient, a Source of Phytoecdysteroids and Flavonoids from Quinoa Grain

2021 ◽  
Vol 37 (5) ◽  
pp. 88-95
Author(s):  
N.A. Petrov ◽  
S.N. Zorin ◽  
N.A. Biryulina ◽  
V.K. Mazo
Keyword(s):  
Wistar Rats ◽  
Immobilization Stress ◽  
Field Tests ◽  
Physical Exertion ◽  
Biologically Active ◽  
Food Sources ◽  
Food Ingredient ◽  
In Vivo Evaluation ◽  
Russian Scientific

Abstract- One of the promising food sources of biologically active substances is quinoa grain, which is valued for its high content of protein, sulfur-containing amino acids, lysine, fiber, and minerals. In addition, quinoa grain can be a valuable food source of polyphenolic compounds and phytoecdysteroids. The method for production of a concentrate of flavonoids and 20-hydroxyecdysone from quinoa grains sorbed on coagulated egg protein has been developed. The in vivo evaluation of efficacy of the developed food ingredient was conducted using male Wistar rats under immobilization stress and after exhausting physical exertion. The consumption of the food ingredient prevented an increase in the level of the main stress markers, catecholamines, in animals subjected to immobilization stress. The opposite effect was observed in animals that received the food ingredient after exhausting physical exertion: their levels of catecholamines were significantly higher than in the rest comparison groups. Using the Elevated Plus Maze and Open Field tests, it was shown that the consumption of the developed concentrate neutralized the negative effect of immobilization stress and treadmill exercise on anxiety in Wistar rats. The results obtained require additional study under conditions of preventive introduction of the food ingredient into the diet of intact animals, as well as a toxicological safety assessment. Key words: quinoa, 20-hydroxyecdysone, flavonoids, stress, immobilization, exhaustive physical exercise, catecholamines, corticosterone This work was supported by the Russian Scientific Foundation, grant no. 19-16-00107.

scholarly journals Noninvasive photoacoustic identification of sentinel lymph nodes containing methylene blue in vivo in a rat model

2008 ◽  
Vol 13 (5) ◽  
pp. 054033 ◽  
Author(s):  
Kwang Hyun Song ◽  
Erich W. Stein ◽  
Julie A. Margenthaler ◽  
Lihong V. Wang
Keyword(s):  
Methylene Blue ◽  
Lymph Nodes ◽  
Rat Model ◽  
Sentinel Lymph Nodes

In vivo Evaluation of Cross-Linked Milk and Wheat Proteins Mediated by Microbial Transglutaminase in White Wistar Rats

2009 ◽  
Vol 4 (3) ◽  
pp. 96-107 ◽  
Author(s):  
Claucia Fernanda Vol Souza ◽  
Janaina Guimaraes ◽  
Simone Hickmann Flores ◽  
Marco Antonio Zachia Ayub
Keyword(s):  
Wistar Rats ◽  
Microbial Transglutaminase ◽  
Wheat Proteins ◽  
In Vivo Evaluation

scholarly journals Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1203 ◽  
Author(s):  
Carlos David Grande Tovar ◽  
Jorge Iván Castro ◽  
Carlos Humberto Valencia ◽  
Diana Paola Navia Porras ◽  
José Herminsul Mina Hernandez ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Biomedical Applications ◽  
Wistar Rats ◽  
Carbon Nanomaterials ◽  
Biological Properties ◽  
High Rate ◽  
Nanocomposite Films ◽  
Scanning Calorimetry ◽  
In Vivo Evaluation

The design of scaffolding from biocompatible and resistant materials such as carbon nanomaterials and biopolymers has become very important, given the high rate of injured patients. Graphene and carbon nanotubes, for example, have been used to improve the physical, mechanical, and biological properties of different materials and devices. In this work, we report the grafting of carbon nano-onions with chitosan (CS-g-CNO) through an amide-type bond. These compounds were blended with chitosan and polyvinyl alcohol composites to produce films for subdermal implantation in Wistar rats. Films with physical mixture between chitosan, polyvinyl alcohol, and carbon nano-onions were also prepared for comparison purposes. Film characterization was performed with Fourier Transformation Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Tensile strength, X-ray Diffraction Spectroscopy (XRD), and Scanning Electron Microscopy (SEM). The degradation of films into simulated body fluid (SBF) showed losses between 14% and 16% of the initial weight after 25 days of treatment. Still, a faster degradation (weight loss and pH changes) was obtained with composites of CS-g-CNO due to a higher SBF interaction by hydrogen bonding. On the other hand, in vivo evaluation of nanocomposites during 30 days in Wistar rats, subdermal tissue demonstrated normal resorption of the materials with lower inflammation processes as compared with the physical blends of ox-CNO formulations. SBF hydrolytic results agreed with the in vivo degradation for all samples, demonstrating that with a higher ox-CNO content increased the stability of the material and decreased its degradation capacity; however, we observed greater reabsorption with the formulations including CS-g-CNO. With this research, we demonstrated the future impact of CS/PVA/CS-g-CNO nanocomposite films for biomedical applications.

In vitro and in vivo evaluation of efficacy of citrate/methylene blue/parabens/IPA solution as a skin disinfectant

2010 ◽  
Vol 60 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Janusz Steczko ◽  
Stephen R. Ash ◽  
Lloyd Brewer ◽  
Al Guillem
Keyword(s):  
Methylene Blue ◽  
In Vivo Evaluation

Methotrexate Aspasomes Against Rheumatoid Arthritis: Optimized Hydrogel Loaded Liposomal Formulation with In Vivo Evaluation in Wistar Rats

2018 ◽  
Vol 19 (3) ◽  
pp. 1320-1336 ◽  
Author(s):  
Saikat Ghosh ◽  
Biswajit Mukherjee ◽  
Shreyasi Chaudhuri ◽  
Tanushree Roy ◽  
Alankar Mukherjee ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Wistar Rats ◽  
Liposomal Formulation ◽  
In Vivo Evaluation

In Vivo Evaluation of Activities and Expression of Antioxidant Enzymes in Wistar Rats Exposed for 90 Days to a Modified Clay

2014 ◽  
Vol 77 (8) ◽  
pp. 456-466 ◽  
Author(s):  
Sara Maisanaba ◽  
María Puerto ◽  
Daniel Gutiérrez-Praena ◽  
María Llana-Ruíz-Cabello ◽  
Silvia Pichardo ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Wistar Rats ◽  
In Vivo Evaluation ◽  
Modified Clay

scholarly journals Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats

2016 ◽  
Vol 89 (2) ◽  
Author(s):  
Henshaw Uchechi Okoroiwu ◽  
Item Justin Atangwho ◽  
Emmanuel Kufre Uko ◽  
Okafor Ifeyinwa Maryann
Keyword(s):  
Wistar Rats ◽  
Ethanol Extract ◽  
Dependent Manner ◽  
Chromolaena Odorata ◽  
Sample Collection ◽  
Leaf Extracts ◽  
In Vivo Evaluation ◽  
Coagulation Study

This study was designed to investigate the effects of aqueous, ethanol and crude extracts of <em>Chromolaena odorata</em> leaf on haemostatic mechanism of wistar rats and its possible <em>in vitro</em> use in coagulation study. Fifty wistar rats of both sexes weighing between 140-180 g were sorted into 10 groups each fed via oral gavage once daily for 21 days. Sample collection was done by cardiac puncture. Bleeding and clotting times were performed using Duke’s and Ivy’s methods, respectively. The prothrombin time was performed using the Quick’s one stage method, while the partial thromboplastin time using kaolin was done using Macpherson and Hardity method using Giess diagnosis reagent. Significant results were observed in the bleeding and clotting times of the three extracts in a dose-dependent manner. The 300-mg/kg ethanol extract decreased the bleeding time more than the other two extracts. Only the ethanol 150 and 300 mg/mL showed <em>in vitro</em> activity. The study showed the <em>in vivo</em> haemostatic properties of <em>Chromolaena odorata</em> leaf extracts and its possible use in <em>in vitro</em> coagulation study.

scholarly journals TOPICAL DELIVERY OF QUERCETIN LOADED TRANSFERSOMES FOR WOUND TREATMENT: IN VITRO AND IN VIVO EVALUATION

2021 ◽  
pp. 189-197
Author(s):  
MARWA ABDALLAH ◽  
DEMIANA I. NESEEM ◽  
OMAIMA N. ELGAZAYERLY ◽  
ALY A. ABDELBARY
Keyword(s):  
Wistar Rats ◽  
Spherical Shape ◽  
The Other ◽  
Wound Treatment ◽  
In Vivo Evaluation ◽  
Skin Deposition ◽  
Significant Difference ◽  
Male Wistar Rats

Objective: To design topical Quercetin (Qc)-loaded transfersomes (TFs) for wound treatment. Methods: Qc-loaded TFs were prepared by thin-film hydration technique using 2241full factorial design and the optimum formula was selected. In vivo skin, deposition and cutaneous wound induction studies were performed for four groups of male wistar rats. At the end of the experiment, biochemical parameters were measured in the healed tissues (total proteins (TP), total antioxidant capacity (TAC), glutathione reductase (GSH), nitric oxide (NO), and malonaldehyde (MDA). Two in vivo histopathological experiments using male wistar rats were performed; the first study was done for the healed tissues of the above experiment and the second was to confirm the safety of formulations. Results: Qc optimum TFs (F6) showed EE% of 91.1%, PS of 695.35 nm, PDI of 0.592, and ZP of-11.1 mV, and spherical shape. In vivo skin deposition study showed that drug percentage retained in the skin from Qc optimum TFs was significantly higher than that from Qc suspension and Qc liposomes (p<0.05). There was no significant difference in the values of TP, TAC and MDA between the treated groups (p>0.05). GSH in TFs treated groups was significantly higher than the other groups (p<0.05) while NO in TFs treated groups was significantly lower than the other treated groups (p<0.05). Histopathological experiments showed that wounds treated by TFs healed better than those treated by both liposomes and Qc suspension. Conclusion: Qc-loaded TFs can be used as successful drug-delivery system for wound healing.

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