scholarly journals Native Endophytic Pseudomonas putida as a Biocontrol Agent against Common Bean Rust Caused by Uromyces appendiculatus

2021 ◽  
Vol 7 (9) ◽  
pp. 745
Author(s):  
Kamal A. M. Abo-Elyousr ◽  
Ismail R. Abdel-Rahim ◽  
Najeeb M. Almasoudi ◽  
Sameera A. Alghamdi
Keyword(s):  
Cell Wall ◽  
Pseudomonas Putida ◽  
Common Bean ◽  
Disease Severity ◽  
Endophytic Bacterium ◽  
Uromyces Appendiculatus ◽  
Molecular Characteristics ◽  
Bean Rust ◽  
Rust Disease

This study aimed to evaluate the efficacy of endophytic bacterium to control common bean rust disease under greenhouse conditions. Endophytic bacterium Pseudomonas putida ASU15 was isolated from fresh asymptomatic common bean, identified using biochemical and molecular characteristics. In vitro, the inhibitory effect of different concentrations of P. putida (1 × 104, 1 × 105 and 1 × 106), as well as fungicide ortiva (0.01%) on uredospores germination of Uromyces appendiculatus were tested using water agar medium. The concentration showing the highest reduction of uredospores germination was at 1 × 106, while there was complete inhibition of uredospores germination associated with using ortiva. Scanning electron microscope exhibited the ability of P. putida cells to attack the cell wall of the fungal uredospores germ tubes of U. appendiculatus, causing obvious cell wall breakdown. The activities of chitinase, lipase, and protease produced by P. putida ASU15, in vitro, were evaluated spectrophotometrically. Chitinolytic, proteolytic, and lipolytic activities were exhibited, contributing 55.26, 3.87, and 26.12 U/mL, respectively. Under greenhouse conditions, treated plants with P. putida ASU15 (two days before pathogen inoculation or at the same time of pathogen inoculation) or fungicide reduced the disease severity, compared to the control. Applying P. putida ASU15 at the same time of pathogen inoculation showed reduction in disease severity (69.9%), higher than application before pathogen inoculation (54.9%). This study is considered the first report that demonstrates the mycoparasitic strategy of P. putida for controlling U. appendiculatus. In conclusion, our results revealed that P. putida ASU15 affords a significant disease reduction that may be attributed to direct suppression of pathogen spores germination.

A Molecular Approach to Distinguish the Bean Rust and Siratro Rust Pathogens

1991 ◽  
Vol 39 (6) ◽  
pp. 527 ◽  
Author(s):  
KS Braithwaite ◽  
JM Manners ◽  
DJ Maclean ◽  
JAG Irwin
Keyword(s):  
Rust Fungus ◽  
Repeat Unit ◽  
Uromyces Appendiculatus ◽  
Bean Rust ◽  
Rust Disease ◽  
Dna Repeat ◽  
Total Dna ◽  
Macroptilium Atropurpureum ◽  
Genomic Probes ◽  
Rust Pathogens

Rust disease on the tropical pasture legume Macroptilium atropurpureum (siratro) is caused by Uromyces appendiculatus var. crassitunicatus. This pathogen was believed to be closely related to the bean (Phaseolus vulgaris) rust pathogen Uromyces appendiculatus var. appendiculatus. The genetic relationship between these two fungi was investigated. Total DNA hybridisations indicated that little homology exists between the high copy genomic DNA of these two rust fungi. Random genomic probes cloned from the bean rust fungus detected extensive Polymorphisms between the two, with only one probe from 17 being monomorphic. The ribosomal DNA repeat unit was also distinguished by RFLPs. It was calculated from the RFLP data that the bean rust fungus and the siratro rust fungus share only 8-14% sequence homology. The results indicate that the two fungi, although morphologically very similar, are not closely related genetically.

scholarly journals Inhibitory effect of antagonistic bacteria against Sclerotium rolfsii, causal agent of southern blight of common bean

2020 ◽  
Vol 39 (1) ◽  
Author(s):  
Juan Carlos Martínez-Álvarez ◽  
Flavio Camacho-Angulo ◽  
Yolani de Jesús Bojórquez-Armenta ◽  
Bardo Sánchez-Soto ◽  
Jesús Damián Cordero-Ramírez ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Common Bean ◽  
Sclerotium Rolfsii ◽  
Inhibitory Effect ◽  
Causal Agent ◽  
Antagonistic Bacteria ◽  
In Planta ◽  
Southern Blight ◽  
Acinetobacter Pittii

El objetivo del presente estudio fue seleccionar cepas bacterianas de la rizósfera del cultivo de frijol y determinar su potencial para el control de <em>Sclerotium rolfsii</em> bajo condiciones <em>in vitro</em> e <em>in planta</em>. Se recolectaron muestras de suelo en los municipios de Ahome, Guasave y Angostura, Sinaloa, México, durante el ciclo agrícola 2018-2019. Los aislados se evaluaron bajo condiciones <em>in vitro</em> y se seleccionaron los mejores para la evaluación <em>in planta</em> e identificación molecular (tres aislados) con base a la región 16S del ADN ribosomal. Se evaluaron 65 aislados bacterianos <em>in vitro</em> contra <em>S. rolfsii</em> con un porcentaje de inhibición de 2.5 a 65%. <em>Acinetobacter pittii </em>(COHUI06)<em>,</em> <em>Pseudomonas putida</em> (SANMI02) y <em>Burkholderia</em> sp. (GLS06) inhibieron 55, 60 y 65% bajo condiciones <em>in vitro</em>; además resultaron no hemolíticas. Solo <em>Burkholderia</em> sp. ejerció mayor porcentaje de inhibición <em>in planta</em> para el control de <em>S. rolfsii,</em> con reducción de incidencia y severidad de la enfermedad en un 40 y 50% respectivamente, y promovió el peso seco de la planta. Para <em>A. pitti</em> o <em>P. putida</em> no fueron eficientes para el control del hongo <em>in planta</em>. Se sugiere hacer estudios con las bacterias en invernadero y campo.

Efficacy of certain chemical compounds on common bean rust disease

2016 ◽  
Vol 49 (19-20) ◽  
pp. 522-532 ◽  
Author(s):  
Mansour M. El-Fawy ◽  
Kamal A. M. Abo-Elyousr
Keyword(s):  
Common Bean ◽  
Chemical Compounds ◽  
Bean Rust ◽  
Rust Disease

scholarly journals RESISTANCE IN COMMON BEAN TO UROMYCES APPENDICULATUS UNDER FIELD AND GREENHOUSE CONDITIONS

2021 ◽  
Vol 19 (2) ◽  
pp. 113-121
Author(s):  
M. Koleva ◽  
Iv. Kiryakov
Keyword(s):  
Common Bean ◽  
Immune Reaction ◽  
Infection Type ◽  
Uromyces Appendiculatus ◽  
Bean Rust ◽  
Sources Of Resistance ◽  
Nonspecific Resistance ◽  
Resistant Phenotype ◽  
Resistant Reaction ◽  
Progress Curve

Bean rust, caused by Uromyces appendiculatus, is a major disease in common bean which occurs annually in The Rhodope Mountains and sporadic in the plains of Bulgaria. The present study aims to find sources of resistance in common bean to the pathogen for using in a breeding program. The reaction of fifty-five Phaseolus vulgaris accessions to the pathogen was monitored under field condition. Infection type, disease intensity and area under the disease progress curve were calculated. Twelve common bean cultivars were inoculated with eight pathotypes of races 20-2, 20-16, and 20-18 in the greenhouse, and infection type was estimated. Twelve accessions had an immune reaction, eight accessions had resistant a reaction, two accessions had a middle resistant reaction, and seven accessions had a susceptible reaction to U. appendiculatus population in both field estimations. Five cultivars showed resistant phenotype to the eight pathotypes in the greenhouse, four of which were resistant in the field (Abritus, Beslet, Trakiya, and Prelom). Five cultivars had a susceptible or resistant reaction to the pathotypes of the same race, resulting from different interaction between resistant genes in the host and virulent genes in the pathogen. Nine accessions showed race-nonspecific resistance in the field expressed in low disease intensity and susceptible/resistant phenotype.

First Report of Bean Rust Caused by Uromyces appendiculatus Race 19-63 in Puerto Rico

2009 ◽  
Vol 10 (1) ◽  
pp. 46 ◽  
Author(s):  
Byron Vega ◽  
James S. Beaver ◽  
Consuelo Estévez de Jensen ◽  
James R. Steadman
Keyword(s):  
Puerto Rico ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Western Hemisphere ◽  
Uromyces Appendiculatus ◽  
Bean Rust ◽  
Phaseolus Vulgaris L ◽  
First Report ◽  
New Race ◽  
Important Disease

Bean rust caused by Uromyces appendiculatus (Pers.) Unger is an important disease in common bean (Phaseolus vulgaris L.) worldwide. To our knowledge this is the first report of a new race of U. appendiculatus (19-63) in Puerto Rico and in the western hemisphere. Accepted for publication 4 February 2009. Published 25 March 2009.

scholarly journals Process-induced cell wall permeability modulates the in vitro starch digestion kinetics of common bean cotyledon cells

2018 ◽  
Vol 9 (12) ◽  
pp. 6544-6554 ◽  
Author(s):  
Andrea Pallares Pallares ◽  
Beatriz Alvarez Miranda ◽  
Ngoc Quynh Anh Truong ◽  
Clare Kyomugasho ◽  
Claire Maria Chigwedere ◽  
...  
Keyword(s):  
Cell Wall ◽  
Common Bean ◽  
Thermal Processing ◽  
Simulated Digestion ◽  
Wall Permeability ◽  
Digestion Kinetics ◽  
Cell Wall Permeability ◽  
Cotyledon Cells ◽  
Kinetics Of

Cell wall barrier role during in vitro simulated digestion of starch in common bean cotyledon cells can be modified through variation of thermal processing intensity.

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