scholarly journals Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

2021 ◽  
Vol 7 (5) ◽  
pp. 386
Author(s):  
Cristina L. Văcar ◽  
Enikö Covaci ◽  
Somsubhra Chakraborty ◽  
Bin Li ◽  
David C. Weindorf ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Ecological Risk ◽  
Absorption Spectrometry ◽  
Dry Weight ◽  
Resistant Species ◽  
Removal Capacity ◽  
Resistance Patterns ◽  
Tolerance Indices ◽  
Resistance Spectrum ◽  
Very High

Filamentous fungi native to heavy metals (HMs) contaminated sites have great potential for bioremediation, yet are still often underexploited. This research aimed to assess the HMs resistance and Hg remediation capacity of fungi isolated from the rhizosphere of plants resident on highly Hg-contaminated substrate. Analysis of Hg, Pb, Cu, Zn, and Cd concentrations by X-ray spectrometry generated the ecological risk of the rhizosphere soil. A total of 32 HM-resistant fungal isolates were molecularly identified. Their resistance spectrum for the investigated elements was characterized by tolerance indices (TIs) and minimum inhibitory concentrations (MICs). Clustering analysis of TIs was coupled with isolates’ phylogeny to evaluate HMs resistance patterns. The bioremediation potential of five isolates’ live biomasses, in 100 mg/L Hg2+ aqueous solution over 48 h at 120 r/min, was quantified by atomic absorption spectrometry. New species or genera that were previously unrelated to Hg-contaminated substrates were identified. Ascomycota representatives were common, diverse, and exhibited varied HMs resistance spectra, especially towards the elements with ecological risk, in contrast to Mucoromycota-recovered isolates. HMs resistance patterns were similar within phylogenetically related clades, although isolate specific resistance occurred. Cladosporium sp., Didymella glomerata, Fusarium oxysporum, Phoma costaricensis, and Sarocladium kiliense isolates displayed very high MIC (mg/L) for Hg (140–200), in addition to Pb (1568), Cu (381), Zn (2092–2353), or Cd (337). The Hg biosorption capacity of these highly Hg-resistant species ranged from 33.8 to 54.9 mg/g dry weight, with a removal capacity from 47% to 97%. Thus, the fungi identified herein showed great potential as bioremediators for highly Hg-contaminated aqueous substrates.

scholarly journals Ecological-Health Risk Assessments of Heavy Metals (Cu, Pb, and Zn) in Aquatic Sediments from the ASEAN-5 Emerging Developing Countries: A Review and Synthesis

Biology ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 7
Author(s):  
Chee Kong Yap ◽  
Khalid Awadh Al-Mutairi
Keyword(s):  
Health Risk ◽  
Ecological Risk ◽  
Dry Weight ◽  
Potential Ecological Risk ◽  
The Philippines ◽  
Reasonable Doubt ◽  
Aquatic Sediments ◽  
Considerable Potential ◽  
Almost All ◽  
Very High

The ASEAN-5 countries (Malaysia, Indonesia, Thailand, Philippines, and Vietnam) of the Association of Southeast Asian Nations as a group is an ever-increasing major economy developmental hub in Asia besides having wealthy natural resources. However, heavy metal (HM) pollution in the region is of increasing environmental and public concern. This study aimed to review and compile the concentrations of Cu, Pb, and Zn in the aquatic sediments of the ASEAN-5 countries published in the literature from 1981 to February 2021. The mean values of Cu, Pb, and Zn in aquatic sediments were elevated and localized in high human activity sites and compared to the earth’s upper continental crust and reference values. Based on 176 reports from 113 publications, the ranges of concentrations (mg/kg dry weight) were 0.09–3080 for Cu, 0.37–4950 for Zn, and 0.07–2666 for Pb. The ecological risk (ER) values ranged from 0.02–1077 for Cu, 0.01–95.2 for Zn, and 0.02–784 for Pb. All reports (100%) showed the Zn ER values were categorized as being between ‘low potential ecological risk’ and ‘considerable potential ecological risk’. Almost all Cu ER values (97.7%) also showed similar ranges of the above two risk categories except for a few reports. The highest Cu level (3080 mg/kg dry weight) was reported from a mine-tailing spill in Marinduque Island of the Philippines with ‘very high ecological risk’. In addition, drainage sediments in the western part of Peninsular Malaysia were categorized as Cu ’high potential ecological risk’. Almost all reports (96%) showed Pb ER values categorized as between ‘low potential ecological risk’ and ‘moderate potential ecological risk’ except for a few reports. Six reports showed Pb ER values of ‘considerable potential ecological risk’, while one report from Semarang (Indonesia) showed Pb ER of ‘very high ecological risk’ (Pb level of 2666 mg/kg dry weight). For the ingestion and dermal contact pathways for sediments from the ASEAN-5 countries, all non-carcinogenic risk (NCR) values (HI values 1.0) for Cu, Pb, and Zn reflected no NCR. The ER and human health risk assessment of Cu, Pb, and Zn were compared in an integrative and accurate manner after we reassessed the HM data mentioned in the literature. The synthesis carried out in this review provided the basis for us to consider Cu, Pb, and Zn as being of localized elevated levels. This provided evidence for the ASEAN-5 group of countries to be considered as being a new socio-economic corridor. Beyond any reasonable doubt, an ever-increasing anthropogenic input of HMs is to be expected to a certain degree. We believe that this paper provides the most fundamental useful baseline data for the future management and sustainable development of the aquatic ecosystems in the region. Lastly, we claim that this review is currently the most up-to-date review on this topic in the literature.

APPLICATION OF AAS AND NAA FOR DETERMINATION OF TIN IN SRMs, AND IN ANIMAL AND HUMAN ORGANS

1992 ◽  
Vol 02 (04) ◽  
pp. 489-491 ◽  
Author(s):  
MOMOKO CHIBA ◽  
VENKATESH G. IYENGAR
Keyword(s):  
Neutron Activation Analysis ◽  
Activation Analysis ◽  
Reference Materials ◽  
Certified Reference Materials ◽  
Analytical Techniques ◽  
Absorption Spectrometry ◽  
Dry Weight ◽  
Mixed Diet ◽  
Human Organs

Tin (Sn) is one of the causative elements of the environmental pollution. As no certified reference materials for Sn are presently available, existing reference materials were analyzed for Sn by two independent analytical techniques; atomic absorption spectrometry (AAS) and neutron activation analysis (NAA). The results obtained by both methods were in agreement except for mixed diet which contains Sn in the range of 50 μ g/g. Further, tin concentrations in human and animal organs have been examined by AAS. Among organs tested tin concentrations in testes were the highest, 2.08±0.62 μ g/g dry weight (mean ±SD, n=12) in humans, and 1.45±0.55 μ g/g (n=8) in mice.

scholarly journals Huperzine A and Huperzine B Production by Prothallus Cultures of Huperzia selago (L.) Bernh. ex Schrank et Mart

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3262
Author(s):  
Wojciech J. Szypuła ◽  
Beata Wileńska ◽  
Aleksandra Misicka ◽  
Agnieszka Pietrosiuk
Keyword(s):  
Pharmaceutical Industry ◽  
Current Source ◽  
Growth Index ◽  
Dry Weight ◽  
Huperzine A ◽  
Biomass Growth ◽  
Tissue Cultures ◽  
In The Wild ◽  
Very High

This is the first report of an efficient and effective procedure to optimize the biosynthesis of huperzine A (HupA) and huperzine B (HupB) in vitro from Huperzia selago gametophytes. Axenic tissue cultures were established using spores collected from the sporophytes growing in the wild. The prothalia were obtained after 7–18 months. Approximately 90 up to 100% of the gametophytes were viable and grew rapidly after each transfer on to a fresh medium every 3 months. The best biomass growth index for prothallus calculated on a fresh (FW) and dry weight (DW) basis, at 24 weeks of culture, was 2500% (FW) and 2200% (DW), respectively. The huperzine A content in the gametophytes was very high and ranged from 0.74 mg/g to 4.73 mg/g DW. The highest yield HupA biosynthesis at >4 mg/g DW was observed on W/S medium without growth regulators at 8 to 24 weeks of culture. The highest HupB content ranged from 0.10 mg/g to 0.52 mg/g DW and was obtained on the same medium. The results demonstrate the superiority of H. selago gametophyte cultures, with the level of HupA biosynthesis approximately 42% higher compared to sporophyte cultures and 35-fold higher than when the alkaloid was isolated from H. serrata, its current source for the pharmaceutical industry. Moreover, the biosynthesis of HupB was several-fold more efficient than in H. selago sporophytes growing in the wild. HPLC-HR-MS analyses of the extracts identified eight new alkaloids previously unreported in H. selago: deacetylfawcettine, fawcettimine, 16-hydroxyhuperzine B, deacetyllycoclavine, annopodine, lycopecurine, des-N-methylfastigiatine and flabelline.

scholarly journals Probabilistic Risk Assessment of Inorganic Arsenic via Consumption of Herbs Collected in Thailand

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Nuntawat Monboonpitak ◽  
Suthep Ruangwises ◽  
Sawanya Buranaphalin ◽  
Nongluck Ruangwises
Keyword(s):  
Risk Assessment ◽  
Arsenic Exposure ◽  
Inorganic Arsenic ◽  
Absorption Spectrometry ◽  
Dry Weight ◽  
Probabilistic Risk Assessment ◽  
Total Arsenic ◽  
Sweet Basil ◽  
Fresh Vegetables ◽  
Probabilistic Risk

Total and inorganic arsenic contents in ten commonly consumed Thai herbs, namely, bird’s eye chili, cayenne pepper, celery, garlic, holy basil, kitchen mint, lemongrass, pepper, shallot, and sweet basil, were determined using atomic absorption spectrometry coupled with a hydride generation system (HG-AAS). Total arsenic contents in fresh herbs and lyophilized herbs ranged from 3.39 to 119 ng/g wet weight (wet wt) and from 41.0 to 156 ng/g dry weight (dry wt), respectively. Inorganic arsenic contents in fresh herbs and lyophilized herbs ranged from 2.09 to 26.9 ng/g (wet wt) and from 23.5 to 55.5 ng/g (dry wt), respectively. Percentages of inorganic arsenic to total arsenic in herbs ranged from 22.7 to 62.0%. High percentages of inorganic arsenic to total arsenic were found in celery, lemongrass and sweet basil. Total arsenic contents in the studied herbs were lower than the maximum limits of Thai and Chinese regulatory standards, set at 2,000 ng/g in foods (excluding aquatic animals and seafood) and 500 ng/g in fresh vegetables, respectively. Total and inorganic arsenic contents in the studied herbs were comparable to or lower than the levels found in other studies in the EU and China. Lifetime average daily dose (LADD) and cancer risk (CR) of inorganic arsenic exposure to commonly consumed herbs were evaluated using probabilistic risk assessment (PRA) by @RISK software version 6.0 of Palisade cooperation. All calculated LADD and CR values from all herbs did not exceed the acceptable levels. It can be concluded that there were very low cancer risks of inorganic arsenic exposure from the consumption of the studied herbs.

scholarly journals Cd and Zn interactions and toxicity in ectomycorrhizal basidiomycetes in axenic culture

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4478 ◽  
Author(s):  
Vinicius H. De Oliveira ◽  
Mark Tibbett
Keyword(s):  
Radial Growth ◽  
Solid Medium ◽  
Axenic Culture ◽  
Dry Weight ◽  
Zinc Toxicity ◽  
Solid Media ◽  
Wide Range ◽  
Ecm Fungi ◽  
Ectomycorrhizal Basidiomycetes ◽  
Tolerance Indices

BackgroundMetal contamination in soils affects both above- and belowground communities, including soil microorganisms. Ectomycorrhizal (ECM) fungi are an important component in belowground community and tolerant strains have great potential in enhancing plant-based remediation techniques. We assessed cadmium and zinc toxicity in five ECM species in liquid media (Hebeloma subsaponaceum;H. cylindrosporum;H. crustuliniforme;Sclerodermasp.;Austroboletus occidentalis) and investigated the potential of Zn to alleviate Cd toxicity. Due to highly divergent results reported in the literature, liquid and solid media were compared experimentally for the first time in terms of differential toxicity thresholds in Cd and Zn interactions.MethodsA wide range of Cd and Zn concentrations were applied to ectomycorrhizal fungi in axenic cultures (in mg L−1): 0; 1; 3; 9; 27; 81; 243 for the Cd treatments, and 0; 1; 30; 90; 270; 810; 2,430 for Zn. Combined Zn and Cd treatments were also applied toH. subsaponaceumandSclerodermasp. Dry weight was recorded after 30 days, and in case of solid medium treatments, radial growth was also measured.Results and DiscussionAll species were adversely affected by high levels of Cd and Zn, andA. occidentaliswas the most sensitive, with considerable biomass decrease at 1 mg L−1Cd, whileSclerodermasp. andH. subsaponaceumwere the most tolerant, which are species commonly found in highly contaminated sites. Cd was generally 10 times more toxic than Zn, which may explain why Zn had little impact in alleviating Cd effects. In some cases, Cd and Zn interactions led to a synergistic toxicity, depending on the concentrations applied and type of media used. Increased tolerance patterns were detected in fungi grown in solid medium and may be the cause of divergent toxicity thresholds found in the literature. Furthermore, solid medium allows measuring radial growth/mycelial density as endpoints which are informative and in this case appeared be related to the high tolerance indices found inH. subsaponaceum.

scholarly journals Determination of trace elements in the plants of Mt. Bozdag, Izmir, Turkey

2010 ◽  
Vol 62 (3) ◽  
pp. 731-738 ◽  
Author(s):  
D. Yildiz ◽  
I. Kula ◽  
G. Ay ◽  
S. Baslar ◽  
Y. Dogan
Keyword(s):  
Trace Elements ◽  
Woody Plants ◽  
Statistical Significance ◽  
Absorption Spectrometry ◽  
Dry Weight ◽  
Current Level ◽  
The Mean ◽  
Herbaceous And Woody Plants ◽  
Made In

The aim of this study was to determine the current level of atmospheric heavy metal content on the Bozdag Mountain of the Aegean Region, Turkey. Twenty nine different plants were selected to study their potential as biomonitors of trace elements such as Ni, Zn, Fe, Pb, Mn and Cd (?g g-1, dry weight). The samples were collected from two different altitudes of Mt. Bozdag. The concentrations of trace elements were determined by atomic absorption spectrometry. The mean concentrations determined at 1000 m altitude ranged from 0.025 to 1.609, 0.232 to 0.731, 0.578 to 5.983, 0.287 to 0.565 and 0.176 to 2.659 (?g g-1, dry weight), for Ni, Zn, Fe, Pb and Mn, respectively. At the altitude of 1600 m, the values ranged from 0.023 to 0.939, 0.258 to 1.254, 0.839 to 5.176, 0.301 to 1.341 and 0.405 to 3.351 (?g g-1, dry weight) for Ni, Zn, Fe, Pb and Mn, respectively. No Cd was detected at either altitude. Statistical significance was determined by the independent sample t-test and comparisons were made in order to determine if there were any differences between the averages of herbaceous and woody plants. .

scholarly journals An 112 Days Experiment on Dietary Cadmium Retention in Hepatopancreas in Adult Cantareus aspersus Snails

2019 ◽  
Vol 70 (8) ◽  
pp. 2803-2804
Author(s):  
George A. Draghici ◽  
Cristina A. Dehelean ◽  
Iulia Pinzaru ◽  
Despina M. Bordean ◽  
Georgeta Pop ◽  
...  
Keyword(s):  
Absorption Spectrometry ◽  
Dry Weight ◽  
Land Snails ◽  
Dependent Manner ◽  
Flame Atomic Absorption ◽  
Wide Range ◽  
Cantareus Aspersus ◽  
Ecotoxicological Model ◽  
Dietary Cadmium ◽  
Kinetics Of

Cadmium is very hazardous pollutant with massive impact on aspects of everyday life. Little knowledge exists on kinetics of dietary cadmium retention in Cantareus aspersus for durations above three months although this species of land snails serves as excellent ecotoxicological model for studying cadmium hazard. Here was used a continuous 112-day exposure study design, in which test snails were exposed to a wide range of dietary cadmium levels, including human- and environmentally-relevant levels. Using Flame Atomic Absorption Spectrometry, cadmium levels in the hepatopancreas were found to increase significantly, and in dose-dependent manner starting from a dietary cadmium dose of 0.2 mg/kg dry weight. The results of the present study render the hepatopancreas of mature snails, C. aspersus, as excellent endpoints for assessing Cd toxicity over a broad range of concentrations. Hence, this species of land snails can be reliably used for both active and passive biomonitoring of environmental cadmium pollution.

Micro-quantity tissue digestion for metal measurements by use of a microwave acid-digestion bomb.

1989 ◽  
Vol 35 (3) ◽  
pp. 488-490 ◽  
Author(s):  
J R Nicholson ◽  
M G Savory ◽  
J Savory ◽  
M R Wills
Keyword(s):  
Nitric Acid ◽  
Reference Material ◽  
Microwave Digestion ◽  
Absorption Spectrometry ◽  
Bovine Liver ◽  
Dry Weight ◽  
Acid Digestion ◽  
Tissue Samples ◽  
Dry Ashing ◽  
Tissue Digestion

Abstract We describe a simple and convenient method for processing small amounts of tissue samples for trace-metal measurements by atomic absorption spectrometry, by use of a modified Parr microwave digestion bomb. Digestion proceeds rapidly (less than or equal to 90 s) in a sealed Teflon-lined vessel that eliminates contamination or loss from volatilization. Small quantities of tissue (5-100 mg dry weight) are digested in high-purity nitric acid, yielding concentrations of analyte that can be measured directly without further sample manipulation. We analyzed National Institute of Standards and Technology bovine liver Standard Reference Material to verify the accuracy of the technique. We assessed the applicability of the technique to analysis for aluminum in bone by comparison with a dry ashing procedure.

scholarly journals Heavy Metals in Wastewater and Sewage Sludge from Selected Municipal Treatment Plants in Eastern Cape Province, South Africa

Water ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2746 ◽  
Author(s):  
Mojeed A. Agoro ◽  
Abiodun O. Adeniji ◽  
Martins A. Adefisoye ◽  
Omobola O. Okoh
Keyword(s):  
Heavy Metals ◽  
South Africa ◽  
Sewage Treatment ◽  
Treatment Plant ◽  
Aquatic Organisms ◽  
Absorption Spectrometry ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
Removal Capacity ◽  
Treatment Facilities

This study assessed the distribution of five heavy metals (Cd, Pb, Cu, Zn, and Fe) across the various stages of treatment in three selected sewage treatment facilities and their receiving waterbodies in the Eastern Cape Province, South Africa. Aqueous and solid (sludge) samples were collected monthly from September 2015 to February 2016. Quantitation was achieved by atomic absorption spectrometry after necessary sample preparations. Concentrations of heavy metal cations in the sludge generally varied from <DL (below detection limit) to 1.17 mg kg−1, <DL to 0.14 mg kg−1, 27.588 to 69.789 mg kg−1, and <DL to 0.099 mg kg−1 for Cu, Cd, Fe and Pb; while Zn was below detection all through. Similarly, the levels of Cu, Cd, and Fe in the influents, effluents, upstream and downstream across the three plants ranged from <DL–6.588 mg L−1, <DL–0.636 mg L−1, <DL–0.878 mg L−1 and <DL–0.711 mg L−1, respectively; Zn and Pb were less than DL in all the matrices and study locations. All the contaminants were below hazardous levels in all the sludge and aqueous samples except Cd which was higher in effluents and surface waters across the board. Wastewater Treatment Plant (WWTP)-A exhibited better removal capacity for Fe (86.6%), compared to WWTP-B (34.7%) and WWTP-C (56.9%). However, the removal of Cu and Zn was very poor in all the treatment facilities studied. Carcinogenic and non-carcinogenic risks evaluated were sufficiently low. This suggests that the levels of contamination, even with respect to Cd, was minimal. Nevertheless, efforts should be made to keep the concentrations of these contaminants at levels safe for humans and aquatic organisms. Furthermore, the use of the effluents from these facilities for irrigation should be discouraged to prevent unnecessary build-up of metals in the soil and plants grown with such, as well as subsequent bioaccumulation and biomagnification in the food chain.

scholarly journals Bioremoval of Different Heavy Metals by the Resistant Fungal StrainAspergillus niger

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Ismael Acosta-Rodríguez ◽  
Juan F. Cárdenas-González ◽  
Adriana S. Rodríguez Pérez ◽  
Juana Tovar Oviedo ◽  
Víctor M. Martínez-Juárez
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Aspergillus Niger ◽  
Fungal Biomass ◽  
Dry Weight ◽  
Removal Capacity ◽  
Removal Of Heavy Metals ◽  
The Ideal

The objective of this work was to study the resistance and removal capacity of heavy metals by the fungusAspergillus niger. We analyzed the resistance to some heavy metals by dry weight and plate: the fungus grew in 2000 ppm of zinc, lead, and mercury, 1200 and 1000 ppm of arsenic (III) and (VI), 800 ppm of fluor and cobalt, and least in cadmium (400 ppm). With respect to their potential of removal of heavy metals, this removal was achieved for zinc (100%), mercury (83.2%), fluor (83%), cobalt (71.4%), fairly silver (48%), and copper (37%). The ideal conditions for the removal of 100 mg/L of the heavy metals were 28°C, pH between 4.0 and 5.5, 100 ppm of heavy metal, and 1 g of fungal biomass.

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