scholarly journals Azole Resistance in Clinical and Environmental Aspergillus Isolates from the French West Indies (Martinique)

2021 ◽  
Vol 7 (5) ◽  
pp. 355
Author(s):  
Lorra Monpierre ◽  
Nicole Desbois-Nogard ◽  
Isabel Valsecchi ◽  
Marielle Bajal ◽  
Cécile Angebault ◽  
...  
Keyword(s):  
West Indies ◽  
Culture Media ◽  
Antifungal Susceptibility ◽  
Resistance Mechanisms ◽  
Clinical Samples ◽  
Azole Resistance ◽  
Environmental Isolates ◽  
French West Indies ◽  
First Time ◽  
Microdilution Broth

The emergence of azole resistant Aspergillus spp., especially Aspergillus fumigatus, has been described in several countries around the world with varying prevalence depending on the country. To our knowledge, azole resistance in Aspergillus spp. has not been reported in the West Indies yet. In this study, we investigated the antifungal susceptibility of clinical and environmental isolates of Aspergillus spp. from Martinique, and the potential resistance mechanisms associated with mutations in cyp51A gene. Overall, 208 Aspergillus isolates were recovered from clinical samples (n = 45) and environmental soil samples (n = 163). They were screened for resistance to azole drugs using selective culture media. The Minimum Inhibitory Concentrations (MIC) towards voriconazole, itraconazole, posaconazole and isavuconazole, as shown by the resistant isolates, were determined using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) microdilution broth method. Eight isolates (A. fumigatus, n = 6 and A. terreus, n = 2) had high MIC for at least one azole drug. The sequencing of cyp51A gene revealed the mutations G54R and TR34/L98H in two A. fumigatus clinical isolates. Our study showed for the first time the presence of azole resistance in A. fumigatus and A. terreus isolates in the French West Indies.

scholarly journals Hypoxylon ochraceotuberosum and Hypoxylon rickii (Ascomycota, Hypoxylaceae) in Las Yungas of Salta province, Northwest of Argentina

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Patricia del Valle Medina ◽  
Eric Kuhnert ◽  
Adriana Inés Hladki ◽  
Esteban Benjamín Sir
Keyword(s):  
West Indies ◽  
Southern Cone ◽  
Montane Forest ◽  
Rain Forests ◽  
French West Indies ◽  
Southeast Brazil ◽  
First Time

Abstract Hypoxylon ochraceotuberosum and H. rickii are reported for the first time for the Argentinean funga based on recently collected specimens from the northwestern montane forest. Until now, H. ochraceotuberosum has only been known to occur in the Martinique (French West Indies). In contrast, H. rickii was already recorded for the Southern Cone, but based on specimens collected in the Atlantic Rain Forests from Southeast Brazil. Detailed descriptions and photo illustrations are provided for each species.

scholarly journals Compare Catalase Activity Between Aspergillus flavus and A. fumigatus, Isolated from Clinical and Environmental Specimens

2020 ◽  
Vol 13 (8) ◽  
Author(s):  
Sara Rouein ◽  
Fatemeh Ghasemi ◽  
Parisa Badiee
Keyword(s):  
Aspergillus Flavus ◽  
Catalase Activity ◽  
Culture Media ◽  
Aspergillus Species ◽  
Clinical Samples ◽  
Environmental Isolates ◽  
Amplex Red ◽  
Signed Rank ◽  
Significant Difference ◽  
Kolmogorov Smirnov

Background: Catalase enzyme is a potential virulence factor for Aspergillus species. Objectives: This study aimed to evaluate the catalase activity in conidium and mycelium of Aspergillus flavus and A. fumigatus isolated from environmental and clinical samples. Methods: Forty Aspergillus species (20 A. fumigatus and 20 A. flavus) were evaluated. Species were identified using the macroscopic and microscopic criteria of the isolates on culture media and the PCR-RFLP method, using the MwoI enzyme. The activity of the enzyme was evaluated using the Amplex red catalase assay kit. The Shapiro Wilk, Kolmogorov-Smirnov, Mann-Whitney, and Wilcoxon Signed Rank tests were used to analyze the data. Results: The mean conidial and mycelial catalase activities in A. flavus clinical and environmental isolates were 58.10, 57.80 mU/mL, and 1328.30, 531.60 mU/mL, respectively. In A. fumigatus clinical and environmental isolates, the activities were 61.10 and 61.40 mU/mL, and 1248.90 and 722.90 mU/mL, respectively. A significant difference was found between conidial and mycelial catalase activity in Aspergillus species (P = 0.01). The mycelial catalase activity of Aspergillus species isolated from clinical samples was higher than the environmental ones (A. flavus P = 0.01 and A. fumigatus P = 0.04). Conclusions: The mycelial catalase activity was higher than conidia. Clinical isolates of A. flavus had similar mycelium activity to A. fumigatus. By using the information provided in the present study, the severity of aspergillosis can be predicted, which paves the way for identifying new antifungal agents.

scholarly journals Epidemiology and Molecular Characterizations of Azole Resistance in Clinical and Environmental Aspergillus fumigatus Isolates from China

2016 ◽  
Vol 60 (10) ◽  
pp. 5878-5884 ◽  
Author(s):  
Yong Chen ◽  
Zhongyi Lu ◽  
Jingjun Zhao ◽  
Ziying Zou ◽  
Yanwen Gong ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Antifungal Susceptibility ◽  
Public Health Problem ◽  
Wide Distribution ◽  
Resistance Mechanisms ◽  
Azole Resistance ◽  
Common Mutation ◽  
Content Type ◽  
Environmental Resistance ◽  
Resistant Strains

ABSTRACTAzole resistance inAspergillus fumigatushas emerged as a worldwide public health problem. We sought here to demonstrate the occurrence and characteristics of azole resistance inA. fumigatusfrom different parts of China. A total of 317 clinical and 144 environmentalA. fumigatusisolates from 12 provinces were collected and subjected to screening for azole resistance. Antifungal susceptibility,cyp51Agene sequencing, and genotyping were carried out for all suspected azole-resistant isolates and a subset of azole-susceptible isolates. As a result, 8 (2.5%) clinical and 2 (1.4%) environmentalA. fumigatusisolates were identified as azole resistant. Five azole-resistant strains exhibit the TR34/L98H mutation, whereas four carry the TR34/L98H/S297T/F495I mutation in thecyp51Agene. Genetic typing and phylogenetic analysis showed that there was a worldwide clonal expansion of the TR34/L98H isolates, while the TR34/L98H/S297T/F495I isolates from China harbored a distinct genetic background with resistant isolates from other countries. High polymorphisms existed in thecyp51Agene that produced amino acid changes among azole-susceptibleA. fumigatusisolates, with N248K being the most common mutation. These data suggest that the wide distribution of azole-resistantA. fumigatusmight be attributed to the environmental resistance mechanisms in China.

scholarly journals cyp51A Mutations, Extrolite Profiles, and Antifungal Susceptibility in Clinical and Environmental Isolates of the Aspergillus viridinutans Species Complex

2019 ◽  
Vol 63 (11) ◽  
Author(s):  
Jessica J. Talbot ◽  
Jens C. Frisvad ◽  
Jacques F. Meis ◽  
Ferry Hagen ◽  
Paul E. Verweij ◽  
...  
Keyword(s):  
Species Complex ◽  
Antifungal Susceptibility ◽  
Gene Mutations ◽  
Azole Resistance ◽  
Wild Type ◽  
Environmental Isolates ◽  
Content Type ◽  
The Past ◽  
Agar Plug ◽  
Very High

ABSTRACT The past decade has seen an increase in aspergillosis in humans and animals due to Aspergillus viridinutans species complex members. Azole resistance is common to these infections, carrying a poor prognosis. cyp51A gene mutations are the main cause of acquired azole resistance in Aspergillus fumigatus. This study aimed to determine if the azole-resistant phenotype in A. viridinutans complex members is associated with cyp51A mutations or extrolite profiles. The cyp51A gene of clinical and environmental isolates was amplified using novel primers, antifungal susceptibility was tested using the Clinical and Laboratory Standards Institute methodology, and extrolite profiling was performed using agar plug extraction. Very high azole MICs were detected in 84% of the isolates (31/37). The MICs of the newer antifungals luliconazole and olorofim (F901318) were low for all isolates. cyp51A sequences revealed 113 nonsynonymous mutations compared to the sequence of wild-type A. fumigatus. M172A/V and D255G, previously associated with A. fumigatus azole resistance, were common among all isolates but were not correlated with azole MICs. Two environmental isolates with nonsusceptibility to itraconazole and high MICs of voriconazole and isavuconazole harbored G138C, previously associated with azole-resistant A. fumigatus. Some novel mutations were identified only among isolates with high azole MICs. However, cyp51A homology modeling did not cause a significant protein structure change for these mutations. There was no correlation between extrolite patterns and susceptibility. For A. viridinutans complex isolates, cyp51A mutations and the extrolites that they produced were not major causes of antifungal resistance. Luliconazole and olorofim show promise for treating azole-resistant infections caused by these cryptic species.

scholarly journals Azole resistance mechanisms in pathogenic M. furfur

2021 ◽  
Author(s):  
Cheryl Leong ◽  
Joel Chan Wai Kit ◽  
Shi Mun Lee ◽  
Yuen In Lam ◽  
Joleen P.Z. Goh ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Systemic Disease ◽  
Antifungal Susceptibility ◽  
Treatment Strategies ◽  
Systemic Infection ◽  
Resistance Mechanisms ◽  
Fungal Species ◽  
Azole Resistance ◽  
In The Wild

Malassezia are emerging fungal pathogens causing opportunistic skin and severe systemic infection. Nosocomial outbreaks are associated with azole resistance and understanding of the underlying mechanisms are limited to knowledge from other fungal species. Herein, we identified distinct antifungal susceptibility patterns in 26 Malassezia furfur isolates derived from healthy and diseased individuals. A Y67F CYP51 mutation was identified in five isolates of M. furfur. However, this mutation alone was insufficient to induce reduce azole susceptibility in the wild type strain. RNA-seq and differential gene analysis of healthy and disease derived strains exposed to clotrimazole in vitro identified several key metabolic pathways and transporter proteins which are involved in reduce azole susceptibility. The pleiotropic drug transporter PDR10 was the single most highly upregulated transporter gene in multiple strains of M. furfur after azole treatment and increased expression of PDR10 is associated with reduced azole susceptibility in some systemic disease isolates of M. furfur. Deletion of PDR10 in a pathogenic M. furfur strain with reduced susceptibility reduced MIC values to the level of that in susceptible isolates. The current dearth of antifungal technologies, globally emerging multi-azole resistance, and broad agriculture and consumer care use of azoles means improved understanding of the mechanisms underlying intrinsic and acquired azole resistance in Malassezia is crucial for development of antibiotic stewardship and antifungal treatment strategies.

scholarly journals Species Identification andIn VitroAntifungal Susceptibility ofAspergillus terreusSpecies Complex Clinical Isolates from a French Multicenter Study

2018 ◽  
Vol 62 (5) ◽  
pp. e02315-17 ◽  
Author(s):  
S. Imbert ◽  
A. C. Normand ◽  
S. Ranque ◽  
J. M. Costa ◽  
J. Guitard ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Antifungal Susceptibility ◽  
Sensu Stricto ◽  
Antifungal Drugs ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Azole Resistance ◽  
University Hospitals ◽  
Content Type ◽  
Interesting Alternative

ABSTRACTAspergillussectionTerreiis a species complex currently comprised of 14 cryptic species whose prevalence in clinical samples as well as antifungal susceptibility are poorly known. The aims of this study were to investigateA. Terreiclinical isolates at the species level and to perform antifungal susceptibility analyses by reference and commercial methods. Eighty-two clinicalA. Terreiisolates were collected from 8 French university hospitals. Molecular identification was performed by sequencing parts of beta-tubulin and calmodulin genes. MICs or minimum effective concentrations (MECs) were determined for 8 antifungal drugs using both EUCAST broth microdilution (BMD) methods and concentration gradient strips (CGS). Among the 79A. Terreiisolates,A. terreus stricto sensu(n= 61),A. citrinoterreus(n= 13),A. hortai(n= 3), andA. alabamensis(n= 2) were identified. All strains had MICs of ≥1 mg/liter for amphotericin B, except for two isolates (bothA. hortai) that had MICs of 0.25 mg/liter. FourA. terreusisolates were resistant to at least one azole drug, including one with pan-azole resistance, yet no mutation in theCYP51Agene was found. All strains had low MECs for the three echinocandins. The essential agreements (EAs) between BMD and CGS were >90%, except for those of amphotericin B (79.7%) and itraconazole (73.4%). Isolates belonging to theA. sectionTerreiidentified in clinical samples show wider species diversity beyond the knownA. terreus sensu stricto. Azole resistance inside the sectionTerreiis uncommon and is not related to CYP51A mutations here. Finally, CGS is an interesting alternative for routine antifungal susceptibility testing.

scholarly journals Comparison of Cryptococcus gattii/neoformans Species Complex to Related Genera (Papiliotrema and Naganishia) Reveal Variances in Virulence Associated Factors and Antifungal Susceptibility

2021 ◽  
Vol 11 ◽  
Author(s):  
Lana Sarita de Souza Oliveira ◽  
Luciana Magalhães Pinto ◽  
Mariana Araújo Paulo de Medeiros ◽  
Dena L. Toffaletti ◽  
Jennifer L. Tenor ◽  
...  
Keyword(s):  
Species Complex ◽  
Antifungal Susceptibility ◽  
Antifungal Drugs ◽  
University Hospital ◽  
Clinical Samples ◽  
Environmental Isolates ◽  
Pathogenic Potential ◽  
Worldwide Distribution ◽  
Clinical Series

Cryptococcosis is an infectious disease of worldwide distribution, caused by encapsulated yeasts belonging to the phylum Basidiomycota. The genus Cryptococcus includes several species distributed around the world. The C. gattii/neoformans species complex is largely responsible for most cases of cryptococcosis. However, clinical series have been published of infections caused by Papiliotrema (Cryptococcus) laurentii and Naganishia albida (Cryptococcus albidus), among other related genera. Here, we examined the pathogenic potential and antifungal susceptibility of C. gattii/neoformans species complex (clades I and II) and related genera (Papiliotrema and Naganishia) isolated from environmental and clinical samples. P. laurentii (clade III), N. liquefasciens/N. albidosimilis (clade IV); and N. adeliensis/N. albida (clade V) strains produced higher levels of phospholipase and hemolysins, whereas the C. gattii/neoformans species complex strains (clades I and II) had markedly thicker capsules, produced more biofilm biomass and melanin, which are known virulence attributes. Interestingly, 40% of C. neoformans strains (clade II) had MICs above the ECV established for this species to amphotericin B. Several non-C. gattii/neoformans species complex (clades III to V) had MICs equal to or above the ECVs established for C. deuterogattii and C. neoformans for all the three antifungal drugs tested. Finally, all the non-C. gattii/neoformans clinical isolates (clades III to V) produced more melanin than the environmental isolates might reflect their particularly enhanced need for melanin during in vivo protection. It is very clear that C. gattii/neoformans species complex (clades I and II) strains, in general, show more similar virulence phenotypes between each other when compared to non-C. gattii/neoformans species complex (clades III to V) isolates. These observations together with the fact that P. laurentii and Naganishia spp. (clades III to V) strains were collected from the outside of a University Hospital, identify features of these yeasts important for environmental and patient colonization and furthermore, define mechanisms for infections with these uncommon pathogens.

scholarly journals First detection of dengue and chikungunya viruses in natural populations of Aedes aegypti in Martinique during the 2013 – 2015 concomitant outbreak

2017 ◽  
Vol 41 ◽  
pp. 1 ◽  
Author(s):  
Laurence Farraudière ◽  
Fabrice Sonor ◽  
Said Crico ◽  
Manuel Étienne ◽  
Laurence Mousson ◽  
...  
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
West Indies ◽  
Natural Populations ◽  
French West Indies ◽  
Aedes Mosquitoes ◽  
Health Authorities ◽  
Public Health Authorities ◽  
First Time

Dengue and chikungunya viruses are transmitted by Aedes mosquitoes. In Martinique, an island of the French West Indies, Aedes aegypti is the suspected vector of both arboviruses; there is no Aedes albopictus on the island. During the concomitant outbreak of 2013 – 2015, the authors collected wild A. aegypti populations, and for the first time, detected dengue and chikungunya viruses in field-collected females. This paper demonstrates the mosquito’s role in transmission of both dengue and chikungunya on the island, and also highlights a tool that public health authorities can use for preventing outbreaks.

scholarly journals Environmental Isolates of Multi-Azole-Resistant Aspergillus spp. in Southern Italy

2018 ◽  
Vol 4 (4) ◽  
pp. 131 ◽  
Author(s):  
Laura Trovato ◽  
Guido Scalia ◽  
Maria Domina ◽  
Salvatore Oliveri
Keyword(s):  
Dna Sequencing ◽  
Southern Italy ◽  
Resistance Mechanism ◽  
Sensu Stricto ◽  
Surveillance Program ◽  
Azole Resistance ◽  
Environmental Isolates ◽  
Related Disease ◽  
First Time ◽  
Aspergillus Section

Azole resistance in Aspergillus spp. has been increasingly reported worldwide. Acquired azole resistance is probably linked to environmental exposure to fungicides used in agriculture. We collected a total of 84 soil and leaf samples from eight farms in Southern Italy. Aspergillus isolates were tested for resistance to itraconazole, posaconazole, and voriconazole by the EUCAST method. Five out of 84 samples yielded A. fumigatus isolates: four of them were itraconazole-resistant and were identified as A. fumigatus sensu stricto, three of them were posaconazole-resistant, and two were also voriconazole-resistant. All three isolates harbored the TR34/L98H resistance mechanism, which was detected by DNA sequencing of the cyp51A gene. Fifteen out of 84 samples yielded Aspergillus spp. isolates and included 11 itraconazole-resistant isolates: Aspergillus section Nigri (9) and Aspergillus section Flavi (2). Our study reports for the first time the isolation of azole-resistant A. fumigatus harboring TR34/L98H mutation from the environment of Southern Italy. The present work provides a better understanding of the magnitude of the environmental spread of azole resistance in the context of a necessary effective surveillance program to improve the management of Aspergillus-related disease.

scholarly journals Aspergillus fumigatus Clinical Isolates Carrying CYP51A with TR34/L98H/S297T/F495I Substitutions Detected after Four-Year Retrospective Azole Resistance Screening in Brazil

2019 ◽  
Vol 64 (3) ◽  
Author(s):  
Laís Pontes ◽  
Caio Augusto Gualtieri Beraquet ◽  
Teppei Arai ◽  
Guilherme Leite Pigolli ◽  
Luzia Lyra ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Tandem Repeat ◽  
Antifungal Susceptibility ◽  
Antifungal Resistance ◽  
Clinical Isolates ◽  
Azole Resistance ◽  
Resistance Screening ◽  
Environmental Isolates ◽  
Content Type ◽  
Susceptibility Tests

ABSTRACT Azole antifungal resistance in Aspergillus fumigatus is a worldwide concern. As in most public hospitals in Brazil, antifungal susceptibility tests are not routinely performed for filamentous fungi at our institution. A 4-year retrospective azole antifungal resistance screening revealed two azole-resistant A. fumigatus clinical isolates carrying the CYP51A TR34 (34-bp tandem repeat)/L98H (change of L to H at position 98)/S297T/F495I resistance mechanism mutations, obtained from two unrelated patients. Broth microdilution antifungal susceptibility testing showed high MICs for itraconazole, posaconazole, and miconazole. Short tandem repeat (STR) typing analysis presented high levels of similarity between these two isolates and clinical isolates with the same mutations reported from the Netherlands, Denmark, and China, as well as environmental isolates from Taiwan. Our findings might indicate that active searching for resistant A. fumigatus is necessary. They also represent a concern considering that our hospital provides tertiary care assistance to immunocompromised patients who may be exposed to resistant environmental isolates. We also serve patients who receive prophylactic antifungal therapy or treatment for invasive fungal infections for years. In these two situations, isolates resistant to the antifungal in use may be selected within the patients themselves. We do not know the potential of this azole-resistant A. fumigatus strain to spread throughout our country. In this scenario, the impact on the epidemiology and use of antifungal drugs will significantly alter patient care, as in other parts of the world. In summary, this finding is an important contribution to alert hospital laboratories conducting routine microbiological testing to perform azole resistance surveillance and antifungal susceptibility tests of A. fumigatus isolates causing infection or colonization in patients at high risk for systemic aspergillosis.

Sign in / Sign up

Export Citation Format

Share Document