Molecular and Environmental Triggering Factors of Pathogenicity of Fusarium oxysporum and F. solani Isolates Involved in the Coffee Corky-Root Disease

Roberto Gamboa-Becerra; Daniel López-Lima; Luc Villain; Jean-Christophe Breitler; Gloria Carrión; Damaris Desgarennes

doi:10.3390/jof7040253

Molecular and Environmental Triggering Factors of Pathogenicity of Fusarium oxysporum and F. solani Isolates Involved in the Coffee Corky-Root Disease

Journal of Fungi ◽

10.3390/jof7040253 ◽

2021 ◽

Vol 7 (4) ◽

pp. 253

Author(s):

Roberto Gamboa-Becerra ◽

Daniel López-Lima ◽

Luc Villain ◽

Jean-Christophe Breitler ◽

Gloria Carrión ◽

...

Keyword(s):

Water Stress ◽

Root Disease ◽

Plant Colonization ◽

Root Knot Nematode ◽

Corky Root ◽

Meloidogyne Spp ◽

High Throughput Phenotyping ◽

Coffee Plants ◽

Combined Infection ◽

Six Genes

Coffee corky-root disease causes serious damages to coffee crop and is linked to combined infection of Fusarium spp. and root-knot nematodes Meloidogyne spp. In this study, 70 Fusarium isolates were collected from both roots of healthy coffee plants and with corky-root disease symptoms. A phylogenetic analysis, and the detection of pathogenicity SIX genes and toxigenicity Fum genes was performed for 59 F. oxysporum and 11 F. solani isolates. Based on the molecular characterization, seven F. oxysporum and three F. solani isolates were assessed for their pathogenicity on coffee seedlings under optimal watering and water stress miming root-knot nematode effect on plants. Our results revealed that a drastic increment of plant colonization capacity and pathogenicity on coffee plants of some Fusarium isolates was caused by water stress. The pathogenicity on coffee of F. solani linked to coffee corky-root disease and the presence of SIX genes in this species were demonstrated for the first time. Our study provides evidence for understanding the pathogenic basis of F. oxysporum and F. solani isolates on coffee and revealed the presence of SIX and Fum genes as one of their pathogenicity-related mechanisms. We also highlight the relevance of chlorophyll, a fluorescence as an early and high-throughput phenotyping tool in Fusarium pathogenicity studies on coffee.

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EFFECTIVENESS OF SOIL SOLARIZATION AND BIOFUMIGATION FOR THE CONTROL OF CORKY ROOT AND ROOT-KNOT NEMATODE MELOIDOGYNE SPP. ON TOMATO

Acta Horticulturae ◽

10.17660/actahortic.2012.933.51 ◽

2012 ◽

pp. 399-405 ◽

Cited By ~ 1

Author(s):

L. Moura ◽

I. Queiroz ◽

I. Mourão ◽

L.M. Brito ◽

J. Duclos

Keyword(s):

Soil Solarization ◽

Root Knot Nematode ◽

Corky Root ◽

Meloidogyne Spp

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Biscogniauxia (Hypoxylon) Canker or Dieback in Trees

EDIS ◽

10.32473/edis-fr407-2017 ◽

2017 ◽

Vol 2017 (6) ◽

Author(s):

Claudia Paez ◽

Jason A. Smith

Keyword(s):

Water Stress ◽

Tree Species ◽

Soil Compaction ◽

Urban Areas ◽

Root Disease ◽

Poor Health ◽

Green Spaces ◽

Opportunistic Pathogens ◽

Wide Range ◽

Quercus Spp

Biscogniauxia canker or dieback (formerly called Hypoxylon canker or dieback) is a common contributor to poor health and decay in a wide range of tree species (Balbalian & Henn 2014). This disease is caused by several species of fungi in the genus Biscogniauxia (formerly Hypoxylon). B. atropunctata or B. mediterranea are usually the species found on Quercus spp. and other hosts in Florida, affecting trees growing in many different habitats, such as forests, parks, green spaces and urban areas (McBride & Appel, 2009). Typically, species of Biscogniauxia are opportunistic pathogens that do not affect healthy and vigorous trees; some species are more virulent than others. However, once they infect trees under stress (water stress, root disease, soil compaction, construction damage etc.) they can quickly colonize the host. Once a tree is infected and fruiting structures of the fungus are evident, the tree is not likely to survive especially if the infection is in the tree's trunk (Anderson et al., 1995).

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Effect of field establishment methods on root-knot nematode (Meloidogyne spp.) infection and growth of Sesbania sesban in western Kenya

Crop Protection ◽

10.1016/s0261-2194(00)00049-1 ◽

2001 ◽

Vol 20 (1) ◽

pp. 31-41 ◽

Cited By ~ 3

Author(s):

J Desaeger ◽

M.R Rao

Keyword(s):

Sesbania Sesban ◽

Root Knot Nematode ◽

Western Kenya ◽

Meloidogyne Spp

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Differential interactions between strains ofRhizorhapis,Sphingobium,SphingopyxisorRhizorhabdusand accessions ofLactucaspp. with respect to severity of corky root disease

Plant Pathology ◽

10.1111/ppa.12188 ◽

2014 ◽

Vol 63 (5) ◽

pp. 1053-1061 ◽

Cited By ~ 5

Author(s):

A. H. C. van Bruggen ◽

O. Ochoa ◽

I. M. Francis ◽

R. W. Michelmore

Keyword(s):

Root Disease ◽

Corky Root

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Genetic diversity of Meloidogyne spp. parasitising potato in Brazil and aggressiveness of M. javanica populations on susceptible cultivars

Nematology ◽

10.1163/15685411-00003032 ◽

2017 ◽

Vol 19 (1) ◽

pp. 69-80 ◽

Cited By ~ 9

Author(s):

Israel L. Medina ◽

Cesar B. Gomes ◽

Valdir R. Correa ◽

Vanessa S. Mattos ◽

Philippe Castagnone-Sereno ◽

...

Keyword(s):

Genetic Diversity ◽

Rapd Markers ◽

Potato Production ◽

Potato Cultivars ◽

Root Knot Nematode ◽

Root Knot Nematodes ◽

Nematode Reproduction ◽

Meloidogyne Spp ◽

Prevalent Species ◽

Reproduction Factor

Root-knot nematodes (Meloidogyne spp.) significantly impact potato production worldwide and in Brazil they are considered one of the most important group of nematodes affecting potatoes. The objectives of this study were to survey Meloidogyne spp. associated with potatoes in Brazil, determine their genetic diversity and assess the aggressiveness of M. javanica on two susceptible potato cultivars. Fifty-seven root-knot nematode populations were identified using esterase phenotyping, including Meloidogyne javanica, M. incognita, M. arenaria and M. ethiopica. Overall, root-knot nematodes were present in ca 43% of sampled sites, in which M. javanica was the most prevalent species, and the phenotypes Est J3, J2a and J2 occurred in 91.2, 6.7 and 2.1% of the positive samples, respectively. Other species, such as M. incognita, M. arenaria and M. ethiopica, were found less frequently and occurred at rates of 6.4, 4.3 and 2.1% of the samples, respectively. Sometimes, M. javanica was found in mixtures with other root-knot nematodes in ca 10.6% of sites containing Meloidogyne. After confirming the identification of 17 isolates of M. javanica and one isolate each of M. incognita, M. arenaria and M. ethiopica by SCAR markers, the populations were used to infer their genetic diversity using RAPD markers. Results revealed low intraspecifc genetic diversity among isolates (13.9%) for M. javanica. Similarly, M. javanica sub-populations (J2a) clustered together (81% of bootstrap), indicating subtle variation from typical J3 populations. The aggressiveness of four populations of M. javanica from different Brazilian states on two susceptible potato cultivars was tested under glasshouse conditions. Results indicated differences in aggressiveness among these populations and showed that potato disease was proportional to nematode reproduction factor.

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USING BIOPESTICIDES FOR THE MANAGEMENT OF ROOT-KNOT NEMATODE, MELOIDOGYNE SPP. ON POTATO

Menoufia Journal of Plant Protection ◽

10.21608/mjapam.2019.118018 ◽

2019 ◽

Vol 4 (3) ◽

pp. 119-120

Author(s):

Zeinab A. El- Bermawy ◽

M. E. M. Sweelam ◽

Sanaa S. Kabeil

Keyword(s):

Root Knot Nematode ◽

Meloidogyne Spp

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The vicious cycle of lettuce corky root disease: effects of farming system, nitrogen fertilizer and herbicide

Plant and Soil ◽

10.1007/s11104-014-2312-5 ◽

2014 ◽

Vol 388 (1-2) ◽

pp. 119-132 ◽

Cited By ~ 11

Author(s):

Ariena H. C. van Bruggen ◽

Isolde M. Francis ◽

Randy Krag

Keyword(s):

Nitrogen Fertilizer ◽

Farming System ◽

Root Disease ◽

Vicious Cycle ◽

Corky Root

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Phytol, a constituent of chlorophyll, induces root-knot nematode resistance in Arabidopsis via the ethylene signaling pathway

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-07-20-0186-r ◽

2020 ◽

Author(s):

Taketo Fujimoto ◽

Hiroshi Abe ◽

Takayuki Mizukubo ◽

Shigemi Seo

Keyword(s):

Nematode Resistance ◽

Ethylene Signaling ◽

Severe Damage ◽

Root Knot Nematode ◽

Chlorophyll Contents ◽

Aerial Parts ◽

Wide Range ◽

Ethylene Signaling Pathway ◽

Meloidogyne Spp ◽

Arabidopsis Mutant

Root-knot nematodes (RKNs; Meloidogyne spp.) parasitize the roots and/or stems of a wide range of plant species, resulting in severe damage to the parasitized plant. The phytohormone ethylene (ET) plays an important role in signal transduction pathways leading to resistance against RKNs. However, little is currently known about the induction mechanisms of ET-dependent RKN resistance. Inoculation of Arabidopsis (Arabidopsis thaliana) roots with RKNs decreased chlorophyll contents in aerial parts of the plant. We observed accumulation of phytol, a constituent of chlorophyll and a precursor of tocopherols, in RKN-parasitized roots. Application of sclareol, a diterpene that has been shown to induce ET-dependent RKN resistance, to the roots of Arabidopsis plants increased phytol contents in roots accompanied by a decrease in chlorophyll in aerial parts. Exogenously applied phytol inhibited RKN penetration of roots without exhibiting nematicidal activity. This phytol-induced inhibition of RKN penetration was attenuated in the ET-insensitive Arabidopsis mutant ein2-1. Exogenously applied phytol enhanced the production of α-tocopherol and expression of VTE5, a gene involved in tocopherol production, in Arabidopsis roots. α-Tocopherol exerted similar induction of RKN resistance as phytol and showed increased accumulation in roots inoculated with RKNs. Furthermore, the Arabidopsis vte5 mutant displayed no inhibition of RKN penetration in response to phytol. These results suggest that exogenously applied phytol induces EIN2-dependent RKN resistance, possibly via tocopherol production.

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