scholarly journals Expanding the Knowledge on the Skillful Yeast Cyberlindnera jadinii

2021 ◽  
Vol 7 (1) ◽  
pp. 36
Author(s):  
Maria Sousa-Silva ◽  
Daniel Vieira ◽  
Pedro Soares ◽  
Margarida Casal ◽  
Isabel Soares-Silva
Keyword(s):  
Regulatory Networks ◽  
Genetic Manipulation ◽  
Food Additives ◽  
Fungal Species ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Core Proteome ◽  
Pharmaceutical Industries ◽  
Valuable Compounds ◽  
Biotechnology Sector

Cyberlindnera jadinii is widely used as a source of single-cell protein and is known for its ability to synthesize a great variety of valuable compounds for the food and pharmaceutical industries. Its capacity to produce compounds such as food additives, supplements, and organic acids, among other fine chemicals, has turned it into an attractive microorganism in the biotechnology field. In this review, we performed a robust phylogenetic analysis using the core proteome of C. jadinii and other fungal species, from Asco- to Basidiomycota, to elucidate the evolutionary roots of this species. In addition, we report the evolution of this species nomenclature over-time and the existence of a teleomorph (C. jadinii) and anamorph state (Candida utilis) and summarize the current nomenclature of most common strains. Finally, we highlight relevant traits of its physiology, the solute membrane transporters so far characterized, as well as the molecular tools currently available for its genomic manipulation. The emerging applications of this yeast reinforce its potential in the white biotechnology sector. Nonetheless, it is necessary to expand the knowledge on its metabolism, regulatory networks, and transport mechanisms, as well as to develop more robust genetic manipulation systems and synthetic biology tools to promote the full exploitation of C. jadinii.

scholarly journals Bioactive Properties of Spirulina: A Review

2021 ◽  
Vol 4 (1) ◽  
pp. 134-142
Author(s):  
Amir Ali Anvar ◽  
◽  
Bahareh Nowruzi ◽  
Keyword(s):  
Single Cell ◽  
Functional Food ◽  
Essential Fatty Acids ◽  
Food Additives ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Essential Nutrients ◽  
Bioactive Properties ◽  
Protein Rich ◽  
Risk Of Cancer

Spirulina is a single-cell protein rich in all essential nutrients and vitamins and can be used to produce functional food. Spirulina is a single-cell protein rich in all essential nutrients and vitamins and can be used to produce functional food. In fact, one of the most important problems in the food industry is the use of synthetic food additives that increase the risk of cancer. Therefore, efforts are being made around the world today to isolate new and safe antioxidants from natural sources. Among these, the natural products of cyanobacteria are an important source of new drug compounds. Natural bioactive products not only have medicinal value themselves but are also used as building models to create synthetic analogs. The chemical composition of Spirulina includes protein (70-55%), carbohydrates (25-25%), essential fatty acids (18%), vitamins, minerals, and pigments such as carotene, chlorophyll A and phycocyanin. Obviously, the introduction of valuable properties of cyanobacteria Spirulina can be a suitable substitute for many antimicrobial compounds and synthetic antioxidants that not only pose no risk to the consumer but can also improve consumer health. In this review, we have discussed the important nutrient, bioactive properties, and immunological applications of Spirulina. The current research suggests that spirulina supplementations have been accepted by global accreditation as a safe nutritional and dietary supplement.

Platelet Aggregation in Rats in Relation to Hyperuricaemia Induced by Dietary Single-Cell Protein and to Protein Deficiency

1978 ◽  
Vol 39 (02) ◽  
pp. 346-359 ◽  
Author(s):  
P D Winocour ◽  
M R Turner ◽  
T G Taylor ◽  
K A Munday
Keyword(s):  
Uric Acid ◽  
Platelet Count ◽  
Platelet Aggregation ◽  
Time Lag ◽  
Blood Platelet ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Low Protein ◽  
Blood Platelet Count ◽  
Rat Platelets

SummaryA major limitation to single-cell protein (SCP) as a human food is its high nucleic acid content, the purine moiety of which is metabolised to uric acid. Rats given a Fusarium mould as a source of SCP in diets containing oxonate, a uricase inhibitor, showed elevated plasma and kidney uric acid concentrations after 21 d, which were related to the level of dietary mould. ADP-induced and thrombin-induced platelet aggregation was greater in the hyperuricaemic rats than in controls and a progressive increase in aggregation with increasing levels of dietary mould was observed. Furthermore a time-lag, exceeding the life-span of rat platelets, was observed between the development of hyperuricaemia and the increase in aggregation. A similar time-lag was observed between the lowering of the hyperuricaemia and the reduction of platelet aggregation when oxonate was removed from the diet.If human platelets react to uric acid in the same manner as rat platelets this might explain the link that has been suggested between hyperuricaemia and ischaemic heart disease. In that event diets high in nucleic acids might be contra-indicated in people at risk from ischaemic heart disease.In rats given a low protein diet (50 g casein/kg) for 21 d ADP-induced and thrombin-induced platelet aggregation and whole blood platelet count were reduced compared with control animals receiving 200 g casein/kg diet but not in rats given 90 or 130 g casein/kg diet. A study of the time course on this effect indicated that the reduction both in aggregation tendency and in whole blood platelet count occurred after 4 d of feeding the low protein diet. These values were further reduced with time.

scholarly journals RNA-Centric Approaches to Profile the RNA–Protein Interaction Landscape on Selected RNAs

2021 ◽  
Vol 7 (1) ◽  
pp. 11 ◽  
Author(s):  
André P. Gerber
Keyword(s):  
Mass Spectrometry ◽  
Protein Interactions ◽  
Regulatory Networks ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Protein Complexes ◽  
Cell Protein ◽  
Transcriptional Regulatory Networks ◽  
Technological Advances

RNA–protein interactions frame post-transcriptional regulatory networks and modulate transcription and epigenetics. While the technological advances in RNA sequencing have significantly expanded the repertoire of RNAs, recently developed biochemical approaches combined with sensitive mass-spectrometry have revealed hundreds of previously unrecognized and potentially novel RNA-binding proteins. Nevertheless, a major challenge remains to understand how the thousands of RNA molecules and their interacting proteins assemble and control the fate of each individual RNA in a cell. Here, I review recent methodological advances to approach this problem through systematic identification of proteins that interact with particular RNAs in living cells. Thereby, a specific focus is given to in vivo approaches that involve crosslinking of RNA–protein interactions through ultraviolet irradiation or treatment of cells with chemicals, followed by capture of the RNA under study with antisense-oligonucleotides and identification of bound proteins with mass-spectrometry. Several recent studies defining interactomes of long non-coding RNAs, viral RNAs, as well as mRNAs are highlighted, and short reference is given to recent in-cell protein labeling techniques. These recent experimental improvements could open the door for broader applications and to study the remodeling of RNA–protein complexes upon different environmental cues and in disease.

Single cell protein as food and feed

Nahrung/Food ◽  
1988 ◽  
Vol 32 (3) ◽  
pp. 219-229 ◽  
Author(s):  
A. Giec ◽  
J. Skupin
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Food And Feed

Single-Cell Protein

1984 ◽  
Vol 2 (1) ◽  
pp. 285-311 ◽  
Author(s):  
R.B. Vasey ◽  
K.A. Powell
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Cell Protein
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