scholarly journals An In Vitro Model for Candida albicans–Streptococcus gordonii Biofilms on Titanium Surfaces

2018 ◽  
Vol 4 (2) ◽  
pp. 66 ◽  
Author(s):  
Daniel Montelongo-Jauregui ◽  
Anand Srinivasan ◽  
Anand Ramasubramanian ◽  
Jose Lopez-Ribot
Keyword(s):  
Candida Albicans ◽  
In Vitro Model ◽  
Streptococcus Gordonii ◽  
Titanium Surfaces ◽  
Vitro Model

scholarly journals An In Vitro Model for Oral Mixed Biofilms of Candida albicans and Streptococcus gordonii in Synthetic Saliva

2016 ◽  
Vol 7 ◽  
Author(s):  
Daniel Montelongo-Jauregui ◽  
Anand Srinivasan ◽  
Anand K. Ramasubramanian ◽  
Jose L. Lopez-Ribot
Keyword(s):  
Candida Albicans ◽  
In Vitro Model ◽  
Streptococcus Gordonii ◽  
Vitro Model

Differential invasion of Candida albicans isolates in an in vitro model of oral candidosis

2004 ◽  
Vol 19 (5) ◽  
pp. 293-296 ◽  
Author(s):  
K. L. Bartie ◽  
D. W. Williams ◽  
M. J. Wilson ◽  
A. J. C. Potts ◽  
M. A. O. Lewis
Keyword(s):  
Candida Albicans ◽  
In Vitro Model ◽  
Oral Candidosis ◽  
Vitro Model

scholarly journals Fusarium and Candida albicans Biofilms on Soft Contact Lenses: Model Development, Influence of Lens Type, and Susceptibility to Lens Care Solutions

2007 ◽  
Vol 52 (1) ◽  
pp. 171-182 ◽  
Author(s):  
Yoshifumi Imamura ◽  
Jyotsna Chandra ◽  
Pranab K. Mukherjee ◽  
Ali Abdul Lattif ◽  
Loretta B. Szczotka-Flynn ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Contact Lens ◽  
Biofilm Formation ◽  
In Vitro Model ◽  
Contact Lenses ◽  
Fungal Keratitis ◽  
Soft Contact ◽  
Soft Contact Lenses ◽  
Vitro Model

ABSTRACT Fungal keratitis is commonly caused by Fusarium species and less commonly by Candida species. Recent outbreaks of Fusarium keratitis were associated with contact lens wear and with ReNu with MoistureLoc contact lens care solution, and biofilm formation on contact lens/lens cases was proposed to play a role in this outbreak. However, no in vitro model for contact lens-associated fungal biofilm has been developed. In this study, we developed and characterized in vitro models of biofilm formation on various soft contact lenses using three species of Fusarium and Candida albicans. The contact lenses tested were etafilcon A, galyfilcon A, lotrafilcon A, balafilcon A, alphafilcon A, and polymacon. Our results showed that clinical isolates of Fusarium and C. albicans formed biofilms on all types of lenses tested and that the biofilm architecture varied with the lens type. Moreover, differences in hyphal content and architecture were found between the biofilms formed by these fungi. We also found that two recently isolated keratitis-associated fusaria formed robust biofilms, while the reference ATCC 36031 strain (recommended by the International Organization for Standardization guidelines for testing of disinfectants) failed to form biofilm. Furthermore, using the developed in vitro biofilm model, we showed that phylogenetically diverse planktonic fusaria and Candida were susceptible to MoistureLoc and MultiPlus. However, Fusarium biofilms exhibited reduced susceptibility against these solutions in a species- and time-dependent manner. This in vitro model should provide a better understanding of the biology and pathogenesis of lens-related fungal keratitis.

scholarly journals A New In Vitro Model to Evaluate Anti-Adhesive Effect against Fungal Nail Infections

2021 ◽  
Vol 11 (5) ◽  
pp. 1977
Author(s):  
Paola Perugini ◽  
Margherita Bonetti ◽  
Marta Guerini ◽  
Giorgio Musitelli ◽  
Pietro Grisoli
Keyword(s):  
Candida Albicans ◽  
Social Life ◽  
In Vitro Model ◽  
Trichophyton Rubrum ◽  
Negative Impact ◽  
Nail Plate ◽  
Negative Control ◽  
Pharmaceutical Product ◽  
Vitro Model

Nail fungal infection is often mistakenly considered a minor issue or a purely esthetic problem that is not worth solving. However, onychomycosis has been demonstrated to have a negative impact on a patient’s social life. Therefore, given the poor efficacy of various therapy types, there is strong interest in exploring new methods for evaluating antifungal treatments. As such, the aim of this work was to develop a new protocol, using bovine hoof membranes as a model of the human nail to evaluate the capability of a product claiming to prevent fungal adhesion, which is the first step of the infection. In this work, two specific and representative fungal strains, Trichophyton rubrum and Candida albicans, were used. In order to evaluate the possible protective activity of a product against fungal contamination of the nail plate, it was first necessary to test the affinity of the hoof membranes to be contaminated by the fungi. Then, a pharmaceutical product and a base coat were tested as a positive and negative control, respectively, by introducing the membranes (anti-fungal, basic or no treatment and single vs. multiple treatments) into the fungal suspensions for three different contact times (15 min, 5 h and 24 h). The results showed that the more significant antiadhesive effect (AE) was obtained against Trichophyton rubrum than against Candida albicans. Furthermore, taking into account the results obtained at all testing times, 5 h appeared to be the best time for testing the antiadhesive activity. The results obtained after three treatments with drugs and on washed membranes, in comparison to one single application of antifungal product (AP), demonstrated clearly that the drug was able to penetrate deeper into the membranes to exert itself, even after washing and also after only 15 min of contact. Thus, hoof membrane has been shown to be a valuable in vitro model for this kind of product assessment.

Assessing the osteogenic potential of zirconia and titanium surfaces with an advanced in vitro model

2019 ◽  
Vol 35 (1) ◽  
pp. 74-86 ◽  
Author(s):  
Markus Rottmar ◽  
Eike Müller ◽  
Stefanie Guimond-Lischer ◽  
Marc Stephan ◽  
Simon Berner ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Osteogenic Potential ◽  
Titanium Surfaces ◽  
Vitro Model

The In Vitro Evaluation of Preosteoblast Migration From 3-D-printed Scaffolds to Decontaminated Smooth and Minimally Rough Titanium Surfaces: A Pilot Study

2021 ◽  
pp. 026119292110221
Author(s):  
Vitor de Toledo Stuani ◽  
David Minjoon Kim ◽  
Masazumi Nagai ◽  
Chia-Yu Chen ◽  
Adriana Campos Passanezi Sant’Ana
Keyword(s):  
Cell Migration ◽  
Pilot Study ◽  
In Vitro Model ◽  
Cellular Migration ◽  
Bone Implant ◽  
Significant Difference ◽  
Animal Use ◽  
Titanium Surfaces ◽  
Vitro Model

In vitro evaluations are essential to gaining a better understanding of re-osseointegration, while reducing animal use and the overall costs of peri-implantitis studies. This pilot study evaluated preosteoblast migration from 3-D-printed scaffolds to decontaminated titanium microimplants, creating a system that tries to mimic the bone–implant interface. Smooth (S) and minimally rough (R) titanium microimplants were incubated in Escherichia coli cultures and divided into six groups according to the decontamination protocol applied: EDTA gel (EDTA); chlorhexidine (CHL); chlorhexidine-soaked gauze (GCHL); scaling (SC); titanium brush (TiB); and implantoplasty (IP). Pristine S and R microimplants were used as the controls (C). After the decontamination procedures, the microimplants were inserted in 3-D-printed polyurethane-based scaffolds previously inoculated with preosteoblast cell cultures. Cellular migration was assessed after 24, 72 and 120 hours by ATP quantification. At the 120-hour time point, there was no statistically significant difference between S-C, S-EDTA, S-CHL, S-GCHL and S-SC ( p > 0.05), and between R-C, R-EDTA and R-GCHL ( p > 0.05). The in vitro model developed in this pilot study successfully demonstrated cell migration on the different decontaminated surfaces. This methodology suggests that on smooth microimplants, EDTA, GCHL, SC and TiB decontamination may have a reduced impact on preosteoblast migration, while on minimally rough microimplants, EDTA and GCHL decontamination affected cell migration the least. However, when selecting a decontamination protocol, the effectiveness of the decontamination per se must also be considered.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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