scholarly journals Reliability and Validity of Near-Infrared Spectroscopy Mitochondrial Capacity Measurement in Skeletal Muscle

2018 ◽  
Vol 3 (2) ◽  
pp. 19 ◽  
Author(s):  
◽  
◽  
Keyword(s):  
Skeletal Muscle ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Reliability And Validity ◽  
Capacity Measurement ◽  
Mitochondrial Capacity

A comparison of exercise type and intensity on the noninvasive assessment of skeletal muscle mitochondrial function using near-infrared spectroscopy

2013 ◽  
Vol 114 (2) ◽  
pp. 230-237 ◽  
Author(s):  
Terence E. Ryan ◽  
Jared T. Brizendine ◽  
Kevin K. McCully
Keyword(s):  
Skeletal Muscle ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Mitochondrial Function ◽  
Rate Constants ◽  
Near Infrared ◽  
Clinical Settings ◽  
Muscle Oxygen ◽  
Muscle Oxygen Consumption ◽  
Intensity Of Exercise

Near-infrared spectroscopy (NIRS) can be used to measure muscle oxygen consumption (mVO2) using arterial occlusions. The recovery rate of mVO2after exercise can provide an index of skeletal muscle mitochondrial function. The purpose of this study was to test the influence of exercise modality and intensity on NIRS measurements of mitochondrial function. Three experiments were performed. Thirty subjects (age: 18–27 yr) were tested. NIRS signals were corrected for blood volume changes. The recovery of mVO2after exercise was fit to a monoexponential curve, and a rate constant was calculated (directly related to mitochondrial function). No differences were found in NIRS rate constants for VOL and ES exercises (2.04 ± 0.57 vs. 2.01 ± 0.59 min−1for VOL and ES, respectively; P = 0.317). NIRS rate constants were independent of the contraction frequency for both VOL and ES (VOL: P = 0.166 and ES: P = 0.780). ES current intensity resulted in significant changes to the normalized time-tension integral (54 ± 11, 82 ± 7, and 100 ± 0% for low, medium, and high currents, respectively; P < 0.001) but did not influence NIRS rate constants (2.02 ± 0.54, 1.95 ± 0.44, 2.02 ± 0.46 min−1for low, medium, and high currents, respectively; P = 0.771). In summary, NIRS measurements of skeletal muscle mitochondrial function can be compared between VOL and ES exercises and were independent of the intensity of exercise. NIRS represents an important new technique that is practical for testing in research and clinical settings.

scholarly journals Home-based aerobic exercise training improves skeletal muscle oxidative metabolism in patients with metabolic myopathies

2016 ◽  
Vol 121 (3) ◽  
pp. 699-708 ◽  
Author(s):  
Simone Porcelli ◽  
Mauro Marzorati ◽  
Lucia Morandi ◽  
Bruno Grassi
Keyword(s):  
Skeletal Muscle ◽  
Infrared Spectroscopy ◽  
Exercise Training ◽  
Near Infrared Spectroscopy ◽  
Oxidative Metabolism ◽  
Near Infrared ◽  
Aerobic Training ◽  
Aerobic Exercise Training ◽  
Low Intensity ◽  
Home Based

Aerobic training can be effective in patients with mitochondrial myopathies (MM) and McArdle's disease (McA). The aim of the study was to use noninvasive functional evaluation methods, specifically aimed at skeletal muscle oxidative metabolism, to evaluate the effects of an aerobic exercise training (cycle ergometer, 12 wk, 4 days/wk, ∼65-70% of maximal heart rate) in 6 MM and 7 McA. Oxygen uptake and skeletal muscle vastus lateralis fractional O2 extraction by near-infrared spectroscopy were assessed during incremental and low-intensity constant work rate (CWR) exercises before (BEFORE) and at the end (AFTER) of training. Peak O2 uptake increased significantly with training both in MM [14.7 ± 1.2 vs. 17.6 ± 1.4 ml·kg−1·min−1 (mean ± SD)] and in McA (18.5 ± 1.8 ml·kg−1·min−1 vs. 21.6 ± 1.9). Peak skeletal muscle fractional O2 extraction increased with training both in MM (22.0 ± 6.7 vs. 32.6 ± 5.9%) and in McA (18.5 ± 6.2 vs. 37.2 ± 7.2%). During low-intensity CWR in both MM and McA: V̇o2 kinetics became faster in AFTER, but only in the patients with slow V̇o2 kinetics in BEFORE; the transient overshoot in fractional O2 extraction kinetics disappeared. The level of habitual physical activity was not higher 3 mo after training (FOLLOW-UP vs. PRE). In MM and McA patients a home-based aerobic training program significantly attenuated the impairment of skeletal muscle oxidative metabolism and improved variables associated with exercise tolerance. Our findings indicate that in MM and McA patients near-infrared spectroscopy and V̇o2 kinetics can effectively detect the functional improvements obtained by training.

scholarly journals Changes in skeletal muscle oxygenation during exercise measured by near-infrared spectroscopy on ascent to altitude

Critical Care ◽  
2009 ◽  
Vol 13 (Suppl 5) ◽  
pp. S7 ◽  
Author(s):  
Daniel S Martin ◽  
Denny ZH Levett ◽  
Michael Mythen ◽  
Mike PW Grocott ◽  
Keyword(s):  
Skeletal Muscle ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Muscle Oxygenation ◽  
Skeletal Muscle Oxygenation

Near infrared spectroscopy-guided exercise training for claudication in peripheral arterial disease

2018 ◽  
Vol 26 (5) ◽  
pp. 471-480 ◽  
Author(s):  
Jonathan R Murrow ◽  
Jared T Brizendine ◽  
Bill Djire ◽  
Hui-Ju Young ◽  
Stephen Rathbun ◽  
...  
Keyword(s):  
Blood Flow ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Muscle Blood Flow ◽  
Arterial Disease ◽  
Walking Program ◽  
Peripheral Arterial ◽  
Walking Time ◽  
Mitochondrial Capacity

Rationale Supervised treadmill exercise for claudication in peripheral arterial disease is effective but poorly tolerated because of ischemic leg pain. Near infrared spectroscopy allows non-invasive detection of muscle ischemia during exercise, allowing for characterization of tissue perfusion and oxygen utilization during training. Objective We evaluated walking time, muscle blood flow, and muscle mitochondrial capacity in patients with peripheral artery disease after a traditional pain-based walking program and after a muscle oxygen-guided walking program. Method and results Patients with peripheral artery disease trained thrice weekly in 40-minute-long sessions for 12 weeks, randomized to oxygen-guided training ( n = 8, age 72 ± 9.7 years, 25% female) versus traditional pain-based training ( n = 10, age 71.6 ± 8.8 years, 20% female). Oxygen-guided training intensity was determined by maintaining a 15% reduction in skeletal muscle oxygenation by near infrared spectroscopy rather than relying on symptoms of pain to determine exercise effort. Pain free and maximal walking times were measured with a 12-minute Gardner treadmill test. Gastrocnemius mitochondrial capacity and blood flow were measured using near infrared spectroscopy. Baseline pain-free walking time was similar on a Gardner treadmill test (2.5 ± 0.9 vs. 3.6 ± 1.0 min, p = 0.5). After training, oxygen-guided cohorts improved similar to pain-guided cohorts (pain-free walking time 6.7 ± 0.9 vs. 6.9 ± 1.1 min, p < 0.01 for change from baseline and p = 0.97 between cohorts). Mitochondrial capacity improved in both groups but more so in the pain-guided cohort than in the oxygen-guided cohort (38.8 ± 8.3 vs. 14.0 ± 9.3, p = 0.018). Resting muscle blood flow did not improve significantly in either group with training. Conclusions Oxygen-guided exercise training improves claudication comparable to pain-based training regimens. Adaptations in mitochondrial function rather than increases in limb perfusion may account for functional improvement. Increases in mitochondrial oxidative capacity may be proportional to the degree of tissue hypoxia during exercise.

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