scholarly journals An Assessment of Cell Culture Plate Surface Chemistry for in Vitro Studies of Tissue Engineering Scaffolds

2015 ◽  
Vol 6 (4) ◽  
pp. 1054-1063 ◽  
Author(s):  
Alexander Röder ◽  
Elena García-Gareta ◽  
Christina Theodoropoulos ◽  
Nikola Ristovski ◽  
Keith Blackwood ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Surface Chemistry ◽  
In Vitro Studies ◽  
Plate Surface ◽  
Tissue Engineering Scaffolds ◽  
Culture Plate

Plasma generated RONS in cell culture medium for in vitro studies of eukaryotic cells on Tissue Engineering scaffolds

2017 ◽  
Vol 14 (11) ◽  
pp. 1700014 ◽  
Author(s):  
Ilaria Trizio ◽  
Vito Rizzi ◽  
Roberto Gristina ◽  
Eloisa Sardella ◽  
Pinalysa Cosma ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Culture Medium ◽  
In Vitro Studies ◽  
Eukaryotic Cells ◽  
Cell Culture Medium ◽  
Tissue Engineering Scaffolds

Preliminary testing of silicone-urethane elastomers as substrates in human cell culture

e-Polymers ◽  
2007 ◽  
Vol 7 (1) ◽  
Author(s):  
Malgorzata Lewandowska-Szumieł ◽  
Janusz Kozakiewicz ◽  
Piotr Mrówka ◽  
Agnieszka Jurkowska ◽  
Edyta Sienkiewicz-Łatka ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Blood Platelets ◽  
Human Fibroblasts ◽  
Medical Implants ◽  
Tissue Engineering Scaffolds ◽  
Xtt Assay ◽  
In Vitro Biocompatibility ◽  
Different Types

AbstractSilicone-urethanes, polymers combining the characteristics of two widely used biomaterials, i.e. polyurethanes and silicones, are highly valued in many applications, including medical implants. To assess properties of these materials in contact with living cells, a set of different silicone-urethane materials, candidates for tissue engineering scaffolds, was synthesized and characterized. Two different oligomeric siloxane diols: Tegomer-2111 (Teg) and KF-6001 (KF), and two different types of diisocyanate, MDI and IPDI, were used in synthesis. Blood platelets adhesion to surfaces of selected materials showed a higher thrombogenicity of material based on Teg. Human fibroblasts were used in in vitro biocompatibility tests. The viability of cells cultured on silicone-urethanes was tested by XTT assay. Teg-based silicone-urethanes showed a significantly higher biocompatibility than those based on KF. Materials based on MDI compared to IPDI were found to be significantly more favoured by cells, not necessarily due to the type of diisocyanate but maybe also because of the necessity of using potentially toxic catalyst which accompanies the use of IPDI. Our studies indicate that silicone-urethanes are potent materials for tissue engineering products development. On the basis of the observations performed in cell culture, Tegomer- 2111 as oligomeric siloxane diol and MDI as diisocyanate are recommended as starting materials for silicone-urethane scaffolds synthesis.

scholarly journals Decellularized Porcine Brain Matrix for Cell Culture and Tissue Engineering Scaffolds

2011 ◽  
Vol 17 (21-22) ◽  
pp. 2583-2592 ◽  
Author(s):  
Jessica A. DeQuach ◽  
Shauna H. Yuan ◽  
Lawrence S.B. Goldstein ◽  
Karen L. Christman
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Tissue Engineering Scaffolds ◽  
Porcine Brain

Preparation, Characterization and In Vitro Release Kinetics of Vancomycin Carried β-TCP/Chitosan Composite Microspheres Used as Bone Tissue Engineering Scaffolds

2012 ◽  
Vol 512-515 ◽  
pp. 1821-1825
Author(s):  
Lin Zhang ◽  
Xue Min Cui ◽  
Qing Feng Zan ◽  
Li Min Dong ◽  
Chen Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Cross Linking ◽  
Tissue Engineering Scaffolds ◽  
Composite Microspheres ◽  
Chitosan Composite ◽  
Vitro Release

A novel microsphere scaffolds composed of chitosan and β-TCP containing vancomycin was designed and prepared. The β-TCP/chitosan composite microspheres were prepared by solid-in-water-in-oil (s/w/o) emulsion cross-linking method with or without pre-cross-linking process. The mode of vancomycin maintaining in the β-TCP/chitosan composite microspheres was detected by Fourier transform infrared spectroscopy (FTIR). The in vitro release curve of vancomycin in simulated body fluid (SBF) was estimated. The results revealed that the pre-cross-linking prepared microspheres possessed higher loading efficiency (LE) and encapsulation efficiency (EE) especially decreasing the previous burst mass of vancomycin in incipient release. These composite microspheres got excellent sphere and well surface roughness in morphology. Vancomycin was encapsulated in composite microspheres through absorption and cross-linking. While in-vitro release curves illustrated that vancomycin release depond on diffusing firstly and then on the degradation ratio later. The microspheres loading with vancomycin would be to restore bone defect, meanwhile to inhibit bacterium proliferation. These bioactive, degradable composite microspheres have potential applications in 3D tissue engineering of bone and other tissues in vitro and in vivo.

Tissue Engineering Scaffolds Based on Photocured Dimethacrylate Polymers for in Vitro Optical Imaging

2006 ◽  
Vol 7 (6) ◽  
pp. 1751-1757 ◽  
Author(s):  
Forrest A. Landis ◽  
Jean S. Stephens ◽  
James A. Cooper ◽  
Marcus T. Cicerone ◽  
Sheng Lin-Gibson
Keyword(s):  
Tissue Engineering ◽  
Optical Imaging ◽  
Tissue Engineering Scaffolds

In Vitro Studies of Chemical Mutagens and Carcinogens. I. Stability Studies in Cell Culture Medium2

1977 ◽  
Vol 59 (3) ◽  
pp. 941-944 ◽  
Author(s):  
Erling M. Jensen ◽  
Robert J. LaPolla ◽  
Paul E. Kirby ◽  
Steve R. Haworth
Keyword(s):  
Cell Culture ◽  
In Vitro Studies ◽  
Stability Studies ◽  
Chemical Mutagens
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