scholarly journals Zebrafish Optomotor Response and Morphology Are Altered by Transient, Developmental Exposure to Bisphenol-A

2021 ◽  
Vol 9 (2) ◽  
pp. 14
Author(s):  
Mikayla Crowley-Perry ◽  
Angelo J. Barberio ◽  
Jude Zeino ◽  
Erica R. Winston ◽  
Victoria P. Connaughton
Keyword(s):  
Bisphenol A ◽  
Age Groups ◽  
Endocrine Disrupting Compounds ◽  
Visual Development ◽  
Visually Guided ◽  
Optomotor Response ◽  
Developmental Exposure ◽  
Treatment Groups ◽  
Long Term Impact ◽  
And Function

Estrogen-specific endocrine disrupting compounds (EDCs) are potent modulators of neural and visual development and common environmental contaminants. Using zebrafish, we examined the long-term impact of abnormal estrogenic signaling by testing the effects of acute, early exposure to bisphenol-A (BPA), a weak estrogen agonist, on later visually guided behaviors. Zebrafish aged 24 h postfertilization (hpf), 72 hpf, and 7 days postfertilization (dpf) were exposed to 0.001 μM or 0.1 μM BPA for 24 h, and then allowed to recover for 1 or 2 weeks. Morphology and optomotor responses (OMRs) were assessed after 1 and 2 weeks of recovery for 24 hpf and 72 hpf exposure groups; 7 dpf exposure groups were additionally assessed immediately after exposure. Increased notochord length was seen in 0.001 μM exposed larvae and decreased in 0.1 μM exposed larvae across all age groups. Positive OMR was significantly increased at 1 and 2 weeks post-exposure in larvae exposed to 0.1 μM BPA when they were 72 hpf or 7 dpf, while positive OMR was increased after 2 weeks of recovery in larvae exposed to 0.001 μM BPA at 72 hpf. A time-delayed increase in eye diameter occurred in both BPA treatment groups at 72 hpf exposure; while a transient increase occurred in 7 dpf larvae exposed to 0.1 μM BPA. Overall, short-term developmental exposure to environmentally relevant BPA levels caused concentration- and age-dependent effects on zebrafish visual anatomy and function.

Screening of pharmaceuticals and endocrine disrupting compounds in water supplies of Cyprus

2010 ◽  
Vol 62 (11) ◽  
pp. 2720-2728 ◽  
Author(s):  
Konstantinos C. Makris ◽  
Shane A. Snyder
Keyword(s):  
Surface Water ◽  
Bisphenol A ◽  
Water Samples ◽  
Potable Water ◽  
Endocrine Disrupting Compounds ◽  
Water Supplies ◽  
Temporal And Spatial Distribution ◽  
Treated Effluent ◽  
Endocrine Disrupting ◽  
Finished Surface

Cyprus is currently the leading country in antibiotic consumption among all European Union member countries and is likely to have a high consumption of pharmaceuticals overall. This reconnaissance type of project sought to investigate the occurrence of 16 pharmaceuticals, six known or suspected endocrine disrupting compounds (EDCs), two flame retardants, one insect repellant, and one fragrance for the first time in water supplies of Cyprus. Groundwater samples from sites that were located beneath farms scattered around Cyprus, wastewater influent and tertiary-treated effluent, raw and finished surface water, and household potable water samples were analyzed using liquid chromatography and tandem mass spectrometry. Most of the tested compounds were < minimum reporting limit, except for ibuprofen (mean of 1.4 ng L−1) and bisphenol A (mean of 50 ng L−1), which were detected in more than one out of the five groundwater sampling sites. Certain compounds were found in large concentrations in the wastewater influent (caffeine 82,000 ng L−1, sulfamethoxazole 240 ng L−1, ibuprofen 4,300 ng L−1, and triclosan 480 ng L−1). However, several pharmaceuticals and EDCs were detected in the tertiary-treated effluent (recycled water). For the raw and finished surface water, and potable water samples, ibuprofen was detected, whereas, bisphenol-A was measured in only potable water. Overall, with a few notable exceptions, source, finished and potable water had rare detection or low concentration of target compounds, but further research is needed to elucidate the temporal and spatial distribution of the detected emerging contaminants along with the characterization of the related public health risk.

scholarly journals Developmental Programming: Prenatal Bisphenol A Induces Non-Monotonic Changes in Epigenetic Modulators in Metabolic Tissues of Female Sheep

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A485-A485
Author(s):  
Muraly Puttabyatappa ◽  
Joseph Norman Ciarelli ◽  
Vasantha Padmanabhan
Keyword(s):  
Adipose Tissue ◽  
Insulin Sensitivity ◽  
Bisphenol A ◽  
Dna Methyltransferases ◽  
Size Analysis ◽  
Developmental Exposure ◽  
Epigenetic Machinery ◽  
The Impact ◽  
Higher Doses ◽  
Female Sheep

Abstract Developmental exposure to endocrine disruptor bisphenol A (BPA) is associated \with metabolic defects during adulthood in the female sheep. These are characterized by peripheral insulin resistance and increase in negative mediators of insulin sensitivity such as oxidative stress in metabolic tissues, lipotoxicity in liver and muscle and adipocyte hypertrophy in visceral (VAT) and subcutaneous (SAT) adipose tissue. Conceivably, developmental impact of BPA on regulators of insulin sensitivity involves changes in epigenetic machinery and mediated via changes in expression of enzymes that induce covalent modifications of DNA and histone. To determine the impact of prenatal BPA exposure on epigenetic enzymes [DNA methyltransferases (DNMT), histone deacetylases (HDAC), histone acetyl transferase EP300, histone methylases (SUV39H1, SMYD3 and EZH2) and histone demethylase KDM1A], metabolic tissues (liver, muscle, VAT and SAT) were collected from 21-month-old female offspring born to mothers treated with 0, 0.05, 0.5, or 5 mg/kg/day of BPA from days 30-90 of gestation. Data were analyzed by Cohen’s effect size analysis and large magnitude differences (Cohens d>0.8) discussed. In liver, prenatal BPA induced: 1) a decrease in DNMT1 and 3B at all doses and DNMT3A at the highest dose, 2) a decrease in histone deacetylase HDAC3 as opposed to increase in acetylase EP300 at the highest dose, 3) a decrease in SUV39H1 at the two higher doses, and 4) an increase in EZH2 only with 0.5 mg dose. The prenatal BPA-induced changes in muscle include: 1) increases in expression of DNMTs and EP300 at all doses, 2) an increase in SUV39H1 at 0.5 mg dose and EZH2 at 0.05 and 0.5 mg doses, and 3) decreases in SMYD3 at the lowest dose and KDM1A with 0.05 and 5 mg doses. Prenatal BPA treatment also induced depot-specific changes at the adipose tissue level. In the VAT prenatal BPA induced: 1) increases in expression of all DNMTs examined 2) increases in HDAC2 at all doses except HDAC3 only at 0.05 and 0.5mg dose and 3) increases in histone acetylase EP300 at all doses. In SAT BPA induced: 1) decrease in DNMT3A at 0.5mg and increase at 5 mg, 2) decreases in HDAC1 and HDAC2 at the lowest dose, 3) an increase in HDAC3 at the medium dose, and 4) a decrease in EP300 at the lowest dose. Contrasting changes in histone methylation modifying enzymes were also evident between VAT and SAT manifested as increases in SUV39H1 at the two higher doses and SMYD3 at all three doses in the VAT as opposed to decrease in SUV39H1 and SMYD3 at 0.05 and 0.5 mg doses and EZH2 and KDM1A at the lowest dose in the SAT. These findings indicating developmental exposure to BPA induces non-monotonic dose responses in epigenetic modifying enzymes are consistent with the premise that changes in epigenetic machinery underlie the metabolic disruptions induced by prenatal BPA treatment likely accounting for the tissue specific changes in insulin sensitivity. (support by R01-ES-016541)

scholarly journals Facile Synthesis of Porous ZnO Nanoparticles Efficient for Photocatalytic Degradation of Biomass-Derived Bisphenol A Under Simulated Sunlight Irradiation

2021 ◽  
Vol 8 ◽  
Author(s):  
Yujie Wang ◽  
Kang Hu ◽  
Zhiyu Yang ◽  
Chenlu Ye ◽  
Xin Li ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Photocatalytic Degradation ◽  
Inorganic Ions ◽  
Endocrine Disrupting Compounds ◽  
Degradation Process ◽  
Sunlight Irradiation ◽  
Calcination Temperatures ◽  
Endocrine Disrupting Compound ◽  
Endocrine Disrupting ◽  
Photocatalytic Removal

Bisphenol A (BPA) produced from biomass is a typical endocrine disrupting compound that is carcinogenic and genotoxic and can be accumulated in water due to its extensive use and difficult degradation. In this study, the porous ZnO photocatalyst with core-shell structure and large surface area was successfully developed for the efficient photocatalytic degradation of BPA. The various effects of calcination temperatures, BPA concentrations, ZnO dosages, pH and inorganic ions on the degradation performance were systemically studied. The results showed that 99% degradation of BPA was achieved in 1 h using the porous ZnO calcined at 550°C under the conditions of 30 mg/L BPA, 1 g/L ZnO, and pH of 6.5. Besides, the inhibition effects of anions for the photocatalytic removal of BPA decreased in the order of H2PO4- > HCO3- > SO42- > Cl−, while the cations K+, Ca2+, and Na+ had little effect on the photocatalytic degradation of BPA. The results of scavenging experiments showed that h+, ·O2-, and e− played the key role in the photocatalytic degradation process. Finally, the main pathways of BPA degradation were proposed based on ten intermediates found in the degradation process. This work may provide a good guideline to degrade various endocrine disrupting compounds in wastewater treatment.

Developmental exposure to low-dose of bisphenol A alters maternal behaviour in rats

2011 ◽  
Vol 205 ◽  
pp. S294
Author(s):  
S. Boudalia ◽  
L. Decocq ◽  
R. Berges ◽  
M. Canivenclavier
Keyword(s):  
Bisphenol A ◽  
Low Dose ◽  
Maternal Behaviour ◽  
Developmental Exposure

When Theatre was for All: the Cork Report, after Ten Years

1996 ◽  
Vol 12 (48) ◽  
pp. 367-383 ◽  
Author(s):  
Ian Brown ◽  
Rob Brannen
Keyword(s):  
Great Britain ◽  
Public Funding ◽  
Senior Lecturer ◽  
Healthy Development ◽  
The Arts ◽  
Art Form ◽  
Long Term Impact ◽  
And Function ◽  
Theatre Management

By the mid 'eighties, the Thatcher government's public funding restrictions had taken a firm hold, leading to a now familiar position of crisis theatre management. In 1985, under pressure from the profession, the Arts Council of Great Britain commissioned an independent enquiry, the first for sixteen years, to evaluate the needs of the publicly funded theatre and to determine funding priorities. Although the resulting Cork Enquiry was seen by many at the time as a cost-cutting exercise, eight months intensive research and evidence-taking led to a carefully constructed case for a funding increase against an estimated shortfall of up to £13.4 million – and also produced a broad vision of the nature of theatre in England. It is now ten years since the Cork Enquiry delivered its report, with the aim of ensuring the healthy development of an art form placed under severe financial constraint. Here lan Brown and Rob Brannen, Secretary and Assistant Secretary to the Enquiry, provide insight into the Enquiry's setting-up, its process, and formulation of recommendations. In the light of recent consultation exercises, they examine the nature and function of such reports alongside the long-term impact of the Cork Enquiry. lan Brown was Drama Director of the Arts Council of Great Britain from 1986 to 1994, and is now Professor and Head of the Drama Department at Queen Margaret College, Edinburgh. Rob Brannen is a Senior Lecturer in Drama at De Montfort University, Bedford.

scholarly journals Neuroendocrine and behavioral effects of maternal exposure to oral bisphenol A in female mice

2014 ◽  
Vol 220 (3) ◽  
pp. 375-388 ◽  
Author(s):  
Lydie Naulé ◽  
Marie Picot ◽  
Mariangela Martini ◽  
Caroline Parmentier ◽  
Hélène Hardin-Pouzet ◽  
...  
Keyword(s):  
Body Weight ◽  
Sexual Behavior ◽  
Bisphenol A ◽  
Preoptic Area ◽  
Third Ventricle ◽  
Daily Intake ◽  
Maternal Exposure ◽  
Developmental Exposure ◽  
Female Mice ◽  
Reference Doses

Bisphenol A (BPA) is a widespread estrogenic compound. We investigated the effects of maternal exposure to BPA at reference doses on sexual behavior and neuroendocrine functions of female offspring in C57BL/6J mice. The dams were orally exposed to vehicle alone or vehicle-containing BPA at doses equivalent to the no observed adverse effect level (5 mg/kg body weight per day) and tolerable daily intake (TDI, 0.05 mg/kg body weight per day) level from gestational day 15 until weaning. Developmental exposure to BPA increased the lordosis quotient in naive females exposed to BPA at the TDI dose only. BPA exposure had no effect on olfactory preference, ability to express masculine behaviors or number of calbindin-positive cells, a sexually dimorphic population of the preoptic area. BPA at both doses selectively increased kisspeptin cell number in the preoptic periventricular nucleus of the rostral periventricular area of the third ventricle in adult females. It did not affect the number of GNRH-positive cells or percentage of kisspeptin appositions on GNRH neurons in the preoptic area. These changes were associated with higher levels of estradiol (E2) at the TDI dose while levels of LH, estrus cyclicity, ovarian and uterine weights, and fertility remained unaffected. Delay in the time of vaginal opening was observed during the postnatal period at TDI dose, without any alteration in body growth. This shows that developmental exposure to BPA at reference doses did not masculinize and defeminize the neural circuitry underlying sexual behavior in female mice. The TDI dose specifically exacerbated responses normally induced by ovarian E2, through estrogen receptor α, during the postnatal/prepubertal period.

scholarly journals Impact of obesity on renal structure and function in the presence and absence of hypertension: evidence from melanocortin-4 receptor-deficient mice

2009 ◽  
Vol 297 (3) ◽  
pp. R803-R812 ◽  
Author(s):  
Jussara M. do Carmo ◽  
Lakshmi S. Tallam ◽  
John V. Roberts ◽  
Elizabeth L. Brandon ◽  
John Biglane ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Renal Injury ◽  
Salt Sensitivity ◽  
Metabolic Abnormalities ◽  
Salt Diet ◽  
Melanocortin 4 Receptor ◽  
Renal Structure ◽  
Long Term Impact ◽  
And Function

The purpose of this study was to determine the long-term impact of obesity and related metabolic abnormalities in the absence and presence of hypertension on renal injury and salt-sensitivity of blood pressure. Markers of renal injury and blood pressure salt sensitivity were assessed in 52- to 55-wk-old normotensive melanocortin-4 receptor-deficient (MC4R−/−) mice and lean C57BL/6J wild-type (WT) mice and in 22-wk-old MC4R−/− and WT mice made hypertensive by NG-nitro-l-arginine methyl ester (l-NAME) in the drinking water for 8 wk. Old MC4R−/− mice were 60% heavier, hyperinsulinemic, and hyperleptinemic but had similar mean arterial pressure (MAP) as WT mice (115 ± 2 and 117 ± 2 mmHg) on normal salt diet (0.4% NaCl). A high-salt diet (4.0% NaCl) for 12 days did not raise MAP in obese or lean mice [ΔMAP: MC4R (−/−) 4 ± 2 mmHg; WT, 2 ± 1 mmHg]. Obese MC4R−/− mice had 23% greater glomerular tuft area and moderately increased GFR compared with WT mice. Bowman's space, total glomerular area, mesangial matrix, urinary albumin excretion (UAE), renal TGF-β and collagen expression were not significantly different between old MC4R−/− and WT mice. Renal lipid content was greater but renal macrophage count was markedly lower in MC4R−/− than WT mice. Mild increases in MAP during l-NAME treatment (∼16 mmHg) caused small, but greater, elevations in UAE, renal TGF-β content, and macrophage infiltration in MC4R−/− compared with WT mice without significant changes in glomerular structure. Thus despite long-term obesity and multiple metabolic abnormalities, MC4R−/− mice have no evidence of renal injury or salt-sensitivity of blood pressure. These observations suggest that elevations in blood pressure may be necessary for obesity and related metabolic abnormalities to cause major renal injury or that MC4R−/− mice are protected from renal injury by mechanisms that are still unclear.

scholarly journals Lack of effect of supplementation with essential fatty acids on bone mineral density in healthy pre- and postmenopausal women:two randomized controlled trials of Efacal® v. calcium alone

2000 ◽  
Vol 83 (6) ◽  
pp. 629-635 ◽  
Author(s):  
E. Joan Bassey ◽  
Julie J. Littlewood ◽  
M. Claire Rothwell ◽  
David W. Pye
Keyword(s):  
Bone Turnover ◽  
Age Groups ◽  
Group Differences ◽  
Mineral Density ◽  
Control Groups ◽  
Treatment Groups ◽  
Markers Of Bone Turnover ◽  
Total Body ◽  
Age Range ◽  
And Control

Randomized controlled trials of the effects of the dietary supplement Efacal® (Scotia Pharmaceuticals Plc, Guildford, Surrey, UK) v. Ca only on total body bone mineral density (BMD) and markers of bone turnover were conducted in healthy pre- and postmenopausal women separately. Total daily dose for 12 months for the Efacal® groups was: Ca 1·0 g, evening primrose oil 4·0 g and marine fish oil 440 mg; and for the control groups was: Ca 1·0 g. Reported compliance was better than 90 % in both age groups. For the forty-three premenopausal women (age range 25–40 years), initial mean total body BMD values were similar for Efacal® and control groups and both groups showed highly significant mean increases of about 1 %; however, there were no significant between-group differences for the changes in BMD or markers of bone turnover. For the forty-two postmenopausal women (age range 50–65 years), initial mean total body BMD values were again well-matched across treatment groups. Both Efacal® and control groups showed highly significant decreases in total body BMD of about 1 %, but again there were no significant between-group differences in total body BMD or markers of bone turnover. Possible confounding variables such as initial total body BMD were explored but had no effect on the outcome in either age group. Nail quality improved in both age groups and in both Efacal® and control groups. Again, there was no significant difference between treatment groups. No evidence was found to support a beneficial effect of Efacal® on BMD in these women.

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