scholarly journals Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach

2019 ◽  
Vol 8 (1) ◽  
pp. 63 ◽  
Author(s):  
Anne Helfen ◽  
Max Masthoff ◽  
Jing Claussen ◽  
Mirjam Gerwing ◽  
Walter Heindel ◽  
...  

Multispectral optoacoustic tomography (MSOT) represents a new imaging approach revealing functional tissue information without extrinsic contrast agents. Using a clinical combined ultrasound (US)/MSOT device, we investigated the interindividual robustness and impact of intra- and interobserver variability of MSOT values in soft tissue (muscle and subcutaneous fat) of healthy volunteers. Semiquantitative MSOT values for deoxygenated (Hb), oxygenated (HbO2) and total hemoglobin (HbT), as well as oxygen saturation (sO2), were calculated for both forearms in transversal and longitudinal probe orientation (n = 3, 8 measurements per subject). For intraobserver reproducibility, the same examiner investigated three subjects twice. Mean values of left vs. right forearm and transversal vs. longitudinal probe orientation were compared using an unpaired Student’s t test. Bland Altmann plots with 95% limits of agreement for absolute averages and differences were calculated. Intraclass correlation coefficients (ICC 2,k) were computed for three different examiners. We obtained reproducible and consistent MSOT values with small-to-moderate deviation for muscle and subcutaneous fat tissue. Probe orientation and body side had no impact on calculated MSOT values (p > 0.05 each). Intraobserver reproducibility revealed equable mean values with small-to-moderate deviation. For muscular tissue, good ICC was obtained for sO2. Measurements of subcutaneous tissue revealed good-to-excellent ICCs for all calculated values. Thus, in this preliminary study on healthy individuals, clinical MSOT provided consistent and reproducible functional soft tissue characterization, independent on the investigating personnel.

2018 ◽  
Vol 34 (4) ◽  
pp. 395-404
Author(s):  
Cedomir Radovic ◽  
Marija Gogic ◽  
Nenad Parunovic ◽  
Dragan Radojkovic ◽  
Radomir Savic ◽  
...  

The study included the progeny of three boar-sires breeds (SL - Swedish Landrace; LW - Large White and P - Pietrain). A total of 201 progeny of both sexes (93 female and 108 male castrated animals), originating from 16 boar-sires, were tested. The study included the progeny of 10 SL boar-sires (sires nuRWer: 1, 2, 3, 7, 8, 9, 15, 16, 17 and 18), progeny of 3 LW sires (sires nuRWer: 4, 5 and 6) and 3 P boar-sires (sires nuRWer 14, 19 and 20), born in four seasons (winter, spring, summer and autumn). Studies have shown that, with an mean weight of a warm carcass side of 81.20 kg, the highest mean values for ham weight (RW; 10.456 kg), mass of intermuscular fatty tissue (RINT; 0.477 kg), ham bone (RB; 0.837 kg) and muscle tissue RMT, 7,939 kg) have progeny of the sires of Pietrain breed (P) compared to SL and LW sires. In comparison to animals sired by SL and LW boars, the progeny of P sires had less skin and subcutaneous fat tissue (RSFT) by 30 and 549 grams. Studies have shown that we have progeny of sires 7 and 9 of SL breed which have the lowest LSMean values for the yield of skin and subcutaneous fat tissue (869 and 876 g), which is below the mean for breed by 364 and 357 g. In addition, when it comes to intermuscular fatty tissue, the lowest established value was recorded in the progeny of sire no. 8 of SL breed (182 g), which is by 220 g less than the general mean and by 132 g below the mean of the sire breed. The animals originating from sires n. 19 and 20 showed the highest weight of muscle tissue (RMT) (8.489 and 8.118 kg) in the ham, which is by 2.853 and 2.482 kg more meat compared to the progeny of sire no. 5 of LW breed. The total weight of the ham and the ham muscle yield were influenced by (P <0.01 and P <0.001) sire breed, sires within the breed, gender and season of birth. A very significant (P <0.001) influence of the weight of warm carcass sides on the ham weight and tissue yield was determined.


2004 ◽  
Vol 47 (4) ◽  
pp. 367-375 ◽  
Author(s):  
T. Kisiel ◽  
J. M. Książkiewicz

Abstract. In two Polish conservative flocks of Miniduck (K2) and Pekin (P33), registered by FAO as domestic genetic resources (World Watch List, 2000) the mean values of physical (tissue components) and qualitative traits of meat (pH15, pH24, colour of meat) were determined. The experimental flocks showed significant variations in body weight at 7 weeks of age (1540 vs 2088 in drakes and 1395 vs 1986 g in ducks), in the proportion of breast and leg muscles and skin with subcutaneous fat in eviscerated carcass. K2 ducks are characterized by outstanding musculature of breast and P33 ducks by that of lower leg and by low fatness. pH15 values of breast and leg muscles in K2 and P33 birds were greater than pH24 of these muscles. In both flocks pH15 values of 6.09 to 6.33 and pH24 values of 5.90 to 6.05 in breast muscles were lower than in leg muscles (6.33 to 6.46 and 6.12 to 6.37, respectively). A significantly lower L* value (darker colour) of breast muscle was characteristic of K2 birds (43.7 in males and 40.5 in females) compared to P33 males and females. Furthermore, breast muscles of K2 drakes and ducks had significantly higher redness (a*) and yellowness values (b*) than P33. In pectoralis superficialis muscle of K2 and P33 females, a significantly lower diameter was found for white muscle fibres (αW) (30.0 to 30.5 μm) and for red muscle fibres (βR) (16.9 to 17.6 μm) than in biceps femoris muscle (αW from 48.3 to 54.2; βR from 36.0 to 37.1 μm). Muscle fibres of K2 compared to P33 ducks were characterized by significantly greater diameters of βR muscle fibre in pectoralis superficialis muscle, and by lower αW and βR fibre diameters in biceps femoris muscle. The experiment showed that meat of ducks from the conservative flocks studied is valuable from the consumer’s point of view.


2021 ◽  
pp. 1465-1472
Author(s):  
Isabel Teo ◽  
Mark Soldin

Subcutaneous body fat is a distinct anatomical entity with unique physiology, metabolism, and disease. The main roles of fat are energy storage, hormone production, and insulation. Free fatty acids and glycerol can be assembled into triglycerides, and conversely triglycerides can undergo lipolysis. Insulin is the key hormone that controls this fine balance. Pregnancy is associated with increased truncal body fat and breast ptosis. Cellulite is the padded appearance of fat and there is currently no single proven treatment. Lipomas are the most common soft tissue tumour and can be treated with excision or liposuction. Lipodystrophies are disorders characterized by the selective loss of body fat and lipofilling is a useful treatment modality. Panniculitis is the inflammation of subcutaneous fat and treatment involves dealing with the precipitating cause. The plastic surgeon should be familiar with the anatomy, physiology, and pathology of fat in order to treat this wide array of adipose-related conditions.


2019 ◽  
Vol 63 (3) ◽  
pp. 479-493 ◽  
Author(s):  
Wadood Abdul ◽  
Ohoud Nafea ◽  
Sanaa Ghouzali

AbstractThere are a number of issues related to the development of biometric authentication systems, such as privacy breach, consequential security and biometric template storage. Thus, the current paper aims to address these issues through the hybrid approach of watermarking with biometric encryption. A multimodal biometric template protection approach with fusion at score level using fingerprint and face templates is proposed. The proposed approach includes two basic stages, enrollment stage and verification stage. During the enrollment stage, discrete wavelet transform (DWT) is applied on the face images to embed the fingerprint features into different directional sub-bands. Watermark embedding and extraction are done by quantizing the mean values of the wavelet coefficients. Subsequently, the inverse DWT is applied to obtain the watermarked image. Following this, a unique token is assigned for each genuine user and a hyper-chaotic map is used to produce a key stream in order to encrypt a watermarked image using block-cipher. The experimentation results indicate the efficiency of the proposed approach in term of achieving a reasonable error rate of 3.87%.


2019 ◽  
Vol 27 (3) ◽  
pp. 230949901988121 ◽  
Author(s):  
Hasan U Oğur ◽  
Rana Kapukaya ◽  
Özgür Külahçı ◽  
Cengiz Yılmaz ◽  
Kemal Yüce ◽  
...  

Aim: To investigate the diagnosis and treatment methods of soft tissue involvement of hydatid cysts (HCs). Materials and Methods: Eleven patients who were diagnosed as having HC with muscular tissue (soft tissue) involvement between 2010 and 2016 were evaluated retrospectively. Seven patients had typical HC magnetic resonance imaging (MRI) and four patients had cysts with an unusual appearance. We evaluated how to diagnose the cysts using imaging methods, their characteristic radiologic images, and treatment alternatives against the disease. The patients were treated with antihelminthic chemotherapy preoperatively and postoperatively. Results: Of the 11 patients who underwent treatment, 7 were diagnosed using MRI and 4 were diagnosed with histopathologic examinations. The mean follow-up period was 16 (range, 6–24) months, and the mean age was 39.4 (range, 24–56) years. In seven patients, multivesicular appearance with specific MRI findings, T2-hypointense rim appearance, double-rim sign, membrane dissociation, and appearance of daughter cysts were identified as diagnostic criteria. Two patients underwent ultrasound assisted percutaneous aspiration–injection–reaspiration (PAIR) treatment. Seven patients underwent total pericystectomy, and two patients underwent subtotal pericystectomy with serum saline injection into the cyst. Two patients showed signs of mild anaphylaxis, one during the diagnosis and one during treatment. Conclusion: There may be difficulties in the diagnosis and treatment of HCs of the musculoskeletal system. It should be known that there are alternative methods in the treatment (cyst excision and PAIR treatment). Clinical, serologic, and radiologic findings should be used in the diagnosis. To avoid complications during the histopathologic diagnosis, MRI should be examined in detail. It is thought that atypical cysts can be diagnosed (double-layer appearance and peripheral rim sign) in addition to typical cysts (detached membrane and multivesicular appearance), and diagnosis and treatment can be planned without anaphylactic complications.


2000 ◽  
Author(s):  
Kornel P. Koestli ◽  
Martin Frenz ◽  
Heinz P. Weber ◽  
Guenther Paltauf ◽  
Heinz Schmidt-Kloiber

Dose-Response ◽  
2005 ◽  
Vol 3 (3) ◽  
pp. dose-response.0 ◽  
Author(s):  
Jouko Tuomisto ◽  
Juha Pekkanen ◽  
Hannu Kiviranta ◽  
Erkki Tukiainen ◽  
Terttu Vartiainen ◽  
...  

A recent case-control study implied an inverse correlation between the measured body burden of dioxins (polychlorinated dibenzo- p-dioxins and polychlorinated dibenzofurans, PCDD/F) and the risk of soft tissue sarcoma in normal population exposed to dioxins mainly via food. The surprising result could not be explained by biases or confounding. There is no a priori confounding by occupational chemicals in a random sample from general population, but exposures to other lipid soluble chemicals with similar sources might be expected to associate with that of dioxins. One such group is polychlorinated biphenyls (PCB). Therefore three most relevant dioxin-like PCB compounds PCB 77, PCB 126, and PCB 169 were now analyzed from the same patients. Cases were 110 soft-tissue sarcoma patients undergoing surgery for their disease, and referents were 227 patients operated for appendicitis. Dioxin and PCB concentrations were analyzed from subcutaneous fat samples by high-resolution gas chromatography—mass spectrometry and TCDD equivalent concentrations (WHO-TEq) were calculated by using toxicity equivalency factors of WHO. The highest risk of sarcoma was found in the septile with the lowest body burden of sum WHO-TEq, and the differences of septiles 2 and 6 from septile 1 were statistically significant. If soft sarcoma risk is true at high occupational levels of dioxins, the provocative result suggests that a possibility of a J-shaped dose-response curve should be taken into consideration and studied further. This is also supported by the similar J-shaped dose responses in animal studies.


1962 ◽  
Vol 58 (3) ◽  
pp. 295-307 ◽  
Author(s):  
E. H. Callow

1. An investigation has been carried out concerning the percentage of fat in the subcutaneous and intermuscular fatty tissues and in the muscular tissues, and the iodine number of the extracted fat from eight anatomical joints (foreshin, neck, shoulder, thorax, loin, pelvis, leg and hindshin) and from the perinephric fatty tissue and psoas muscles of the carcasses from twenty-four animals. The animals were from three breeds (Hereford, Dairy Shorthorn and Friesian) and were on four levels of nutrition—high-high and medium-high, and high-medium and medium-medium. The first two groups—finished on concentrates—were younger than the second two groups—finished on grass.2. Using an analysis of variance for the resultant data for percentage of fat and iodine number, it was found that breed had affected the percentage of fat in the tissues of the various joints significantly—on the average the order was Shorthorn (highest), Hereford and Friesian (lowest). This result could be predicted from the fact that this was the order of fatness of the carcasses (as measured by the percentage of fatty tissue in the carcass—see Callow, 1961).3. The data for iodine number showed no significant effect for breed in either the subcutaneous or intermuscular tissues. The significant effect of breed in the case of muscle could be attributed to significant differences in the overall level of fatness (see Callow, 1961) of the carcasses of the three breeds.4. A good correlation existed between the average percentage of fat in a joint and the average iodine number of fat extracted when the data for various joints was used to calculate straight lines of best fit. (In the case of muscular tissue, it was necessary to use the inverse of the percentage of fat to calculate the average—because the relation between the percentage of fat in muscular tissue and its iodine number is a rectangular hyperbola.)5. Using such lines of best fit, it was possible to show that certain joints had tissues which gave abnormal values for iodine number. Thus, deepseated tissues, like psoas muscle and kidney fat, had unexpectedly low values, whilst tissues from the hindshin had unexpectedly high values. This was attributed to the effect of local temperature—a high local temperature in the body giving a lower iodine number and a low temperature a higher iodine number than would otherwise be expected. The special case of subcutaneous fat from the thorax—with a higher iodine number than expected—is explained by the presence of brisket fat (which has a high iodine number).6. The data were used graphically to show the effect of growth gradients, thus the average percentage of fat in all the tissues is lowest in fore- and hindshins and highest in thorax (muscular and intermuscular tissues) and in the pelvis (subcutaneous tissue). Similar effects, but in the reverse direction, were shown by average values for iodine numbers.7. There were systematic differences in the percentage of fat in the subcutaneous and intermuscular tissues of joints and in the iodine number of the extracted fat. These too showed marked growth gradients. Although the percentage of fat in the intermuscular tissue was greater than that in the subcutaneous tissue in the fore- and hindshins, it was less in the thorax, loin, pelvis and leg. In spite of this, the iodine number of the subcutaneous fat was always higher than that of the intermuscular fat. This is attributed to the former being a colder tissue than the latter.8. It was shown that the relation between iodine number and percentage of fat in the various joints was different for the animals finished on grass (highmoderate and moderate-moderate levels of nutrition) as compared with those finished on concentrates (high-high and moderate-high levels). This was attributed to the rate of fattening being greater in the second case and to this giving rise to lower iodine numbers.9. Beyond this effect of rate of fattening, no reason could be found for the fact that the general level of iodine numbers showed variation from animal to animal.10. The extreme variation in percentage of fat in the tissues was (a) muscular tissue from 1·3 to 14·5%, (b) intermuscular tissue from 29·5 to 82·7%, and (c) subcutaneous tissue from 25·2 to 89·8%. For iodine number the variation was (a) 50·0 to 73·9, (b) 42·9 to 67·9 and (c) 46·3 to 67·9 It is thus clear that beef can be a very variable foodstuff.


1975 ◽  
Vol 84 (2) ◽  
pp. 281-290 ◽  
Author(s):  
J. L. L'Estrange ◽  
T. A. Mulvihill

SUMMARYTwo surveys, involving 288 lambs, were carried out on the effects of system of production on the melting (slip) point, iodine value, and fatty acid composition of perinephric and subcutaneous carcass fat. The systems studied were:A, early Spring lambs fattened indoors on concentrates;B, early Spring lambs off grass;C, early Summer lambs off grass;D, Autumn lambs off grass, andE, Winter hoggets.The melting point of both perinephric and subcutaneous fat increased progressively during the season from mean values of 41 and 32°C respectively in group A to mean values of 48 and 42°C respectively in group E. Discolouration of the surface fat was associated with the low melting points of group A.Mean iodine values of both fats decreased progressively from group A to group E and this change was associated with a decrease in levels of oleic and palmitoleic acid and a corresponding increase in the level of stearic acid. In the fat, levels of myristic acid were highest in grass fed lambs while levels of linoleic acid were highest in the concentrate-fed lambs.Within each group, perinephric fat contained more stearic and less oleic, palmitoleic, palmitic and myristic acid than subcutaneous fat.Within animals, the levels between perinephric and subcutaneous fat of melting point, iodine value and most of the individual fatty acids were highly correlated.Within animals also, the melting point of perinephric fat was correlated in decreasing order of significance with stearic acid, oleic acid, iodine value, linoleic acid and palmitoleic acid, while melting point of subcutaneous fat was correlated in decreasing order of significance with stearic acid, oleic acid, palmitoleic acid, iodine value, linoleic acid and total branched-chain fatty acids.


2018 ◽  
Vol 39 (6) ◽  
pp. 2727
Author(s):  
Leandro Bren ◽  
Paulo Rossi Junior ◽  
José Luis Moletta ◽  
Eduardo Michelon do Nascimento ◽  
Sergio Rodrigo Fernandes ◽  
...  

Improving the quality and energy content of rations increases performance in feedlot cattle, resulting in higher weight at slaughter, better carcass quality and a higher yield of non-carcass components. The purpose of this study was to evaluate the effect of increasing concentrate content in the ration on the carcass traits and weight of non-carcass components of young bulls finished in a feedlot. Eighteen young crossbred ½ Purunã x ½ Canchim bulls were used, with an average body weight (BW) of 278 kg and 12 months of age at the start of the experiment. The experimental design was completely randomized with three treatments and six replicates, where the treatments were the contents of 0.71, 0.97 and 1.24% BW day-1 of concentrate in the ration, on a dry matter (DM) basis. Corn silage was used as roughage and was provided ad libitum during the experiment. The young bulls were kept in the feedlot for 168 days and slaughtered at 17 months of age. There was a linear effect of concentrate content on the slaughter weight (SW) and hot carcass weight (HCW), which increased from 433 to 485 kg and from 248 to 280 kg between 0.71 and 1.24% BW in DM day-1 of concentrate. This represented an increase of 9.87 kg in SW and 6.16 kg in HCW for each 0.10% BW in DM day-1 of concentrate added to the ration. The hot carcass yield, rib eye area, subcutaneous fat thickness and the proportions of bone, muscle and fat in the carcass were not affected by the concentrate content and showed mean values of 57.3%, 79.6 cm2, 3.20 mm, 14.88%, 65.21% and 20.67%, respectively. There was a linear effect of concentrate content on the carcass temperature after cooling, which increased from 8.1 to 9.0 oC between 0.71 and 1.24% BW in DM day-1 of concentrate. An increase of 0.16 oC in the post-cooling carcass temperature for each 0.10% BW in DM day-1 of concentrate added to the ration was observed. The weight of internal organs and the perirenal and internal fat deposition were not affected by the concentrate content. The inclusion of 1.24% BW in DM day-1 of concentrate in the ration is recommended to obtain high SW and high HCW. Below this level, the carcass tissue composition and weight of non-carcass components are not modified.


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