scholarly journals Decreased Sarcopenia in Aged Females with Young Ovary Transplants was Preserved in Mice that Received Germ Cell-Depleted Young Ovaries

2019 ◽  
Vol 8 (1) ◽  
pp. 40 ◽  
Author(s):  
Tracy L. Habermehl ◽  
Jeffrey B. Mason
Keyword(s):  
Body Composition ◽  
Germ Cell ◽  
Positive Influence ◽  
Test Results ◽  
Control Groups ◽  
Health Span ◽  
Treatment Groups ◽  
Significant Difference ◽  
Aging Health ◽  
Grip Test

Previously, transplantation of young, cycling, ovaries increased life and health span in post-reproductive female mice. The current study addressed the influence of ovarian germ cells in the improvement in health by performing transplantations of young, germ cell-depleted ovaries. The purpose of this study is to further the understanding of reproductive influences on aging health. Control mice were grouped by age. Treatment mice were age-matched and received either germ cell depleted ovaries or germ cell containing ovaries at 400 days of age. All groups underwent health span assays until sacrifice (treatment and age-matched control groups were between 680 and 700 days). Body composition results displayed an improvement of body composition in both treatment groups, compared to the controls, but no significant difference between the germ cell-depleted or germ cell-containing groups. Grip test results showed no improvement in musculoskeletal endurance and no change to mild loss of grip strength with both transplant groups compared to control groups. The research presented here suggests that reproductive status has a positive influence in post-reproductive health. A portion of this influence may be germ cell independent.

Survival of Macrobrachium amazonicum embryos submitted to cooling

Zygote ◽  
2017 ◽  
Vol 25 (3) ◽  
pp. 288-295 ◽  
Author(s):  
Arthur Vinícius Lourenço Ferreira ◽  
Moisés Fernandes Martins ◽  
Míriam Luzia Nogueira Martins de Sousa ◽  
Aldeney Andrade Soares Filho ◽  
Célia Maria de Souza Sampaio
Keyword(s):  
Survival Rate ◽  
Storage Time ◽  
Survival Rates ◽  
Negative Control ◽  
Control Groups ◽  
Treatment Groups ◽  
Significant Difference ◽  
Macrobrachium Amazonicum ◽  
Cryoprotectant Solution ◽  
Positive Controls

SummaryCooling techniques have several applications for reproduction in aquaculture. However, few studies have sought to create protocols for cooling and cryopreservation of Macrobrachium amazonicum embryos. Thus, the objective of this work was to verify the survival of M. amazonicum embryos and the correlation between embryonic volume and mortality of M. amazonicum embryos after cooling. Embryo pools were collected from three females and divided into two treatment groups: dimethyl sulfoxide (DMSO) 3% and ethylene glycol (EG) 0.5%, both of them associated with 2 M sucrose. Positive and negative control groups consisted of seawater 10%. Aliquots of 10 µg of embryos were placed in Falcon® tubes containing a cryoprotectant solution and submitted directly to the test temperature of 2°C for 2 and 6 h of cooling. Further analysis of survival and embryonic volume were performed under a stereoscopic microscope. Data were subjected to analysis of variance (ANOVA), and means were compared using the Tukey test at 5%. The highest embryonic survival rate was observed after the shortest storage time for both the DMSO 3% and the 0.5% EG groups, with survival rates of 84.8 ± 3.9 and 79.7 ± 2.8%, respectively. There was a reduction in survival after 24 h, with the DMSO 3% group presenting a survival rate of 71.7 ± 6.6%, and the EG 0.5% group, 66 ± 6.9%. Survival showed a statistically significant difference when compared with the positive controls after 2 h and 24 h of cooling, with 99 ± 0.5% and 95.8 ± 1.5% survival rates, respectively. There was no significant statistical difference in the embryonic volume, but it was possible to observe a change in the appearance of the embryos, from a translucent coloration to an opaque white or brownish coloration, after 24 h in incubators. Thus, it can be concluded that survival is inversely proportional to storage time and that, although there was no change in the embryonic volume after cooling, a change in the appearance of embryos could be observed.

scholarly journals THE EFFECT OF A PURIFIED SOURCE OF CELLULOSE ON THE GROWTH AND BODY COMPOSITION OF GROWING PIGS

1961 ◽  
Vol 41 (1) ◽  
pp. 120-125 ◽  
Author(s):  
H. M. Cunningham ◽  
D. W. Friend ◽  
J. W. G. Nicholson
Keyword(s):  
Body Composition ◽  
Iodine Number ◽  
Feed Efficiency ◽  
Nutritive Value ◽  
Statistical Significance ◽  
Growing Pigs ◽  
Control Groups ◽  
Significant Difference ◽  
Ad Libitum ◽  
Basal Ration

One hundred pigs were used in two experiments to determine the nutritive value of a purified source of cellulose (solka-floc) and its effect on body composition and iodine number of the loin fat. In each experiment one group of pigs received a basal ration ad libitum while levels of 10 and 40 per cent solka floe were added to the basal ration of two groups at 62 and 162 pounds liveweight, respectively. The pigs given solka-floc were fed ad libitum and the amounts of basal ration consumed were pair-fed to two respective control groups. There was a significant decrease (P <.01) in dressing percentage of pigs fed solka-floc. Feed efficiency, based on total feed (less solka-floc) per pound of gain, was better in the pair-fed controls reaching statistical significance (P <.05) in one experiment. There was no significant difference in the protein or fat content of the carcasses but the iodine number of the loin fat was significantly higher (P <.01) in pigs fed solka-floc. It was concluded that in growing pigs there is no net gain in energy from the consumption of cellulose.

scholarly journals Wine Oligosaccharides: Underutilized or Irrelevant? A Study into the Effects of Oligosaccharides on Wine Taste and Mouthfeel

2021 ◽  
Vol 10 (5) ◽  
pp. 60
Author(s):  
Samuel Hoffman ◽  
Quynh Phan ◽  
Elizabeth Tomasino
Keyword(s):  
Phenolic Compounds ◽  
Descriptive Analysis ◽  
Test Results ◽  
Direct Effects ◽  
Treatment Groups ◽  
Complex Interactions ◽  
Low Concentrations ◽  
Significant Difference ◽  
Model Matrix ◽  
Wine Matrix

The taste and mouthfeel of a wine are two of the most important aspects of wine tasting. However, while much is known about phenolic compounds and other macromolecules direct effects on wine taste and mouthfeel, little is known about other wine compounds such as oligosaccharides. This experiment uses Fructo-oligosaccharide (FOS) and Galacto-oligosaccharide (GOS) at two different concentrations, 450 mg/L and 900 mg/L within a simple model wine matrix. A model matrix was used to control for any unknown interactions between oligosaccharides and the multitude of wine components. Oligosaccharides were added individually to the model wine matrix at each concentration to create four treatments. Triangle tests were performed on all treatments against the control base model wine and between the high and low concentrations of each oligosaccharide treatment. Following the triangle tests, each treatment and the control underwent descriptive analysis (DA) using line intensity scales for sweetness, bitterness, astringency, acidity, and viscosity. Triangle test results revealed a significant difference only between the FOS450 and FOS900 samples. The wine matrix was made more complicated by adding polyphenols and still, none of the four oligosaccharide treatment groups were found to be significantly different. DA found no significant differences for the five attributes but did show clear trends in increased sweetness and acidity, decreased bitterness, as well as changes to astringency and viscosity. This suggests there may be more complex interactions happening within the mouth. However, given the lack of significant results in the simple wine model and the more complex wine model wine, any complex interactions between oligosaccharides and other wine compounds are likely to be minimal.

scholarly journals The pore size of chitosan-Aloe vera scaffold and its effect on VEGF expressions and woven alveolar bone healing of tooth extraction of Cavia cobaya

2020 ◽  
Vol 53 (3) ◽  
pp. 115
Author(s):  
S. Sularsih
Keyword(s):  
Treatment Group ◽  
Pore Size ◽  
Bone Healing ◽  
Tooth Extraction ◽  
Alveolar Bone ◽  
Histopathological Examination ◽  
Aloe Vera ◽  
Control Groups ◽  
Treatment Groups ◽  
Significant Difference

Background: Pore size of scaffolds affects cellular activity, stimulates angiogenetic factors of vascular endothelial growth factor (VEGF), synthesises new blood vessels to regulate migration and proliferation, and accelerates alveolar bone healing of tooth extraction. Purpose: This study aims to analyse the pore size of chitosan-Aloe vera scaffold and its effects on VEGF expression and woven alveolar bone healing of tooth extraction of Cavia cobaya. Methods: 36 male Cavia cobaya, aged 3-3.5 months were divided into six groups: negative control groups (without scaffold), positive control groups (chitosan scaffold), and treatment groups (chitosan-Aloe vera scaffold) on 7- and 14-day observations. Histopathological examination was performed to account the woven alveolar bone areas, and immunohistochemical examination was conducted to examine VEGF expressions on endothelial cells. Data was analysed using a one-way analysis of variance (ANOVA) and least significant difference (LSD) test (p<0.05). Scaffold pore size examination was performed with scanning electron microscope (SEM) with 250x and 500x magnification. Results: Chitosan-Aloe vera scaffold was found to have open pore interconnectivity, the largest pore size was 138.9 μm, while the smallest was 110.5 μm and average pore size was 134.85 μm. The highest expression of VEGF was observed in the treatment group on days 7 (11.5 ±1.39)  and 14 (15.28±1.78), while the largest woven alveolar bone was observed in the treatment group on days 7(17.83±1.47) and 14 (37.67±3.65). Statistically, there was a significant difference between control groups and the treatment groups (p=0.000; p<0.05). Conclusion: Chitosan-Aloe vera scaffold has pore characteristics increasing VEGF expressions and woven alveolar bone areas.

Efficacy of Imagery and Meditation Techniques in Treating Psoriasis

1989 ◽  
Vol 8 (1) ◽  
pp. 25-38 ◽  
Author(s):  
Louise Gaston ◽  
Jean-Charles Crombez ◽  
Jacques Joly ◽  
Sheilagh Hodgins ◽  
Marc Dumont
Keyword(s):  
Time Series ◽  
Multivariate Regression ◽  
Multivariate Regression Analysis ◽  
Control Group ◽  
Control Groups ◽  
Treatment Groups ◽  
Series Design ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Using a dismantled time-series design, eighteen subjects were randomly assigned to three groups: meditation ( N = 5); meditation and imagery ( N = 4); waiting list ( N = 5). Treatment lasted twelve weeks, while pre- and post-baseline periods were of four weeks. An assessment control group control was also used during baseline periods ( N = 4). The severity of psoriasis symptoms was reliably rated by dermatologists. While controlling for extraneous factors influencing psoriasis symptoms, a time-series multivariate regression analysis revealed a significant difference between the mean psoriasis ratings of treatment and control groups after treatment (partial r = .30, p < .01). No additional impact was associated with the use of the imagery technique (partial r = − .06, p > .05). Four subjects in the treatment groups, two in each condition, succeeded in clinically improving their psoriasis symptoms, and none deteriorated. The practice of mediation may be clinically effective for some patients in reducing their psoriasis symptoms.

scholarly journals Toothpaste Prevents Debonded Brackets on Erosive Enamel

2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Érico Luiz Damasceno Barros ◽  
Shelon Cristina Souza Pinto ◽  
Alvaro Henrique Borges ◽  
Mateus Rodrigues Tonetto ◽  
Roger Phillip Ellwood ◽  
...  
Keyword(s):  
Bond Strength ◽  
Clinical Relevance ◽  
Strength Test ◽  
Distilled Water ◽  
Control Groups ◽  
Treatment Groups ◽  
Significant Difference ◽  
High Fluoride ◽  
Fluoride Dentifrice ◽  
Acid Challenge

This study evaluated the effect of high fluoride dentifrice on the bond strength of brackets after erosive challenge. Eighty-four enamel specimens were divided into seven groups(n=12): WN (distilled water/no acid challenge), W3C (distilled water/3 cycles of acid challenge), and W6C (distilled water/6 cycles of acid challenge) were not submitted to dentifrice treatment. Groups RF3C (regular fluoride dentifrice/3 cycles of acid challenge) and RF6C (regular fluoride dentifrice/6 cycles of acid challenge) were treated with dentifrices containing 1450 μg F−/g and HF3C (high fluoride dentifrice/3 cycles of acid challenge) and HF6C (high fluoride dentifrice/6 cycles of acid challenge) were with 5000 μg F−/g. Acid challenges were performed for seven days. After bond strength test, there was no significant difference among groups submitted to 3 cycles of acid challenge(P>0.05). Statistically significant difference was found between the regular and high fluoride dentifrices after 6 cycles of acid challenge (<0.05). Similar areas of adhesive remaining were found among control groups and among groups W6C, RF3C, RF6C, HF3C, and HF6C. The high fluoride dentifrice was able to prevent the reduction of bond strength values of brackets submitted to acid challenge. Clinical relevance: the high fluoride toothpaste prevents debonded brackets on erosive enamel.

Comparison of Students Taught Basketball Skills Using Mastery and Nonmastery Learning Methods

1992 ◽  
Vol 11 (3) ◽  
pp. 235-247 ◽  
Author(s):  
Connie L. Blakemore ◽  
H. Gill Hilton ◽  
Joyce M. Harrison ◽  
Tracy L. Pellett ◽  
James Gresh
Keyword(s):  
Instructional Strategy ◽  
Mastery Learning ◽  
Control Group ◽  
Psychomotor Skills ◽  
Control Groups ◽  
Treatment Groups ◽  
Competitive Game ◽  
Game Situation ◽  
Significant Difference ◽  
And Control

Mastery learning is an instructional strategy that embraces the philosophy that almost any student can learn what is being taught given sufficient time and help. Through Bloom’s group-based, teacher-paced model, 71 seventh-grade boys were taught basketball skills. Students in two treatment groups (mastery and nonmastery) and a control group were compared on the performance of psychomotor skills in isolation and in a competitive game situation before, midway through, and following their training. Students in the mastery group were not taught new skills until 80% had mastered the present skills. The mastery group performed significantly better on isolated skills than did the nonmastery and control groups. There was no significant difference between groups in the performance of skills in a competitive game situation.

scholarly journals Impact of Buprenorphine on Learning and Memory Ability Related to the Acetylcholinesterase Change in the Hippocampus of Rats

2021 ◽  
Vol 11 (5) ◽  
pp. 13111-13114
Keyword(s):  
Memory Impairment ◽  
Ache Activity ◽  
Memory Function ◽  
Male Rats ◽  
Control Groups ◽  
Treatment Groups ◽  
Activity Inhibition ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Impact

Buprenorphine (BUP), a “synthetic opioid”, may cause memory impairment. This investigation aimed to study the impact of BUP on memory function related to acetylcholinesterase (AChE) activity inhibition in male rats. 24 male Wistar rats were randomly divided into three groups; control (C) and two treatment groups BUP (0.3 and 1) (n=8, for each group). BUP (0.3 and 1 mg/kg) was administrated subcutaneously once a day for 30 days. Normal saline 0.9% was injected in to control groups. In the end, animals were anesthetized and decapitated, and their hippocampus was dissected to assess AChE activity. There were no significant differences between the activities of AChE in the hippocampus in BUP-treated animals compared with controls. Besides, the activities of AChE in the BUP 0.3 group and BUP 1 group did not indicate a significant difference. These findings did not confirm the effect of BUP at doses of 0.3 and 1 mg/kg on memory function associated with the AChE activity inhibition.

scholarly journals Improving Insomnia in Primary Care Patients a Randomized Controlled Trial of Nurse-Led Group Treatment: A Literature Review

2020 ◽  
Vol 1 (1) ◽  
pp. 19-24
Author(s):  
Alwan Revai
Keyword(s):  
Primary Care ◽  
Health Care ◽  
Primary Health Care ◽  
Group Treatment ◽  
Control Groups ◽  
Stockholm County ◽  
Treatment As Usual ◽  
Treatment Groups ◽  
Primary Health ◽  
Significant Difference

Introduction: Insomnia is generally defined as complaints of poor or unsatisfactory sleep, which can include difficulty initiating sleep, awakening after sleep onset, poor sleep quality, early morning awakenings, short sleep times, short total sleep times and daytime consequences. Chronic insomnia is a common problem in primary health care, affecting 10 - 19% of those seeking primary care. Method: Patient is a randomly selected sample of 165 people and divided into 2 groups, namely 90 treatment groups and 75 control groups in seven routine primary health care in Stockholm County, Sweden between August 2011 and June 2014. The intervention to be given was in the treatment group given the intervention consisting of a group treatment program for insomnia based on the CBT-I technique while the control group was free to undergo insomnia treatment as usual. Result: The formulation of the research problem is to compare how the effect of nurse-led group treatment with the effect of treatment as usual in routine primary health care in Stockholm County, Sweden. In this study, the researcher determined a sample of 165 people who met the research requirements, and divided respondents randomly (single-blinded) consisting of 90 treatment groups and 75 control groups. Conclusion: There was a significant difference between the treatment of insomnia with cognitive therapy and the treatment of insomnia with drugs. So that we can reduce the long-term effects of using drugs to treat insomnia.

Antibacterial Effects of Calotropisgigantea (L) Extracts on Porphyromonasgingivalis

2020 ◽  
Vol 48 ◽  
pp. 111-118
Author(s):  
Zulfan M. Alibasyah ◽  
D.S. Ningsih ◽  
Sunnati ◽  
R. Andayani ◽  
N.P. Ranggaswuni
Keyword(s):  
Ethanol Extract ◽  
Dilution Method ◽  
Test Results ◽  
Treatment Groups ◽  
Significant Difference ◽  
The One ◽  
Dominant Bacteria ◽  
Post Hoc ◽  
Potential Test ◽  
Antibacterial Potential

The Porphyromonasgingivalis is one of the dominant bacteria that causes chronic periodontitis. These bacteria can penetrate the gingiva and cause tissue damage directly or indirectly with the induction of inflammation. The growth of Porphyromonasgingivalis can be inhibited by administrating the active compounds from plants that have an antibacterial effect such as Calotropisgigantea leaves.This study aimed to determine the antibacterial potential of Bidurileaves (Calotropisgigantea) extract against the Porphyromonasgingivalis ATCC. In this study the Biduri leaves were extracted by maceration method using 70% ethanol solvent. The antibacterial potential test of 70% ethanol extract of Biduri leaves (with a concentration of 5%, 10%, 15%, 20%, 25%, 30%, and 35%) against thePorphyromonasgingivalis was carried out using dilution method with 4 times repetition on Tryptone Soy Agar (TSA) media.The results of the study were analyzed using the One Way ANOVA test showing there were significant differences in the number of Porphyromonasgingivalis ATCC 33277 colonies due to administration of 70% ethanol extract of Bidurileaves (Calotropisgigantea) with p values <0,05. The test results were continued with the Post Hoc Tamhane test to determine the significant difference of the treatment groups. Based on the results of the study it could be concluded that 70% ethanol extract of Biduri leaves (Calotropisgigantea) had antibacterial potential against Porphyromonasgingivalis ATCC 33277 starting from the smallest concentration of 5% to the highest concentration of 35%.

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