scholarly journals Regeneration of Transected Recurrent Laryngeal Nerve Using Hybrid-Transplantation of Skeletal Muscle-Derived Stem Cells and Bioabsorbable Scaffold

2018 ◽  
Vol 7 (9) ◽  
pp. 276 ◽  
Author(s):  
Akihito Kazuno ◽  
Daisuke Maki ◽  
Ippei Yamato ◽  
Nobuyuki Nakajima ◽  
Hiroya Seta ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Recurrent Laryngeal Nerve ◽  
Green Fluorescence Protein ◽  
Immunohistochemical Analysis ◽  
Laryngeal Nerve ◽  
Multipotent Stem Cells ◽  
Enhanced Expression ◽  
Endoneurial Cells ◽  
Pga Felt

Hybrid transplantation of skeletal muscle-derived multipotent stem cells (Sk-MSCs) and bioabsorbable polyglyconate (PGA) felt was studied as a novel regeneration therapy for the transected recurrent laryngeal nerve (RLN). Sk-MSCs were isolated from green fluorescence protein transgenic mice and then expanded and transplanted with PGA felt for the hybrid transplantation (HY group) into the RLN transected mouse model. Transplantation of culture medium (M group) and PGA + medium (PGA group) were examined as controls. After eight weeks, trans-oral video laryngoscopy demonstrated 80% recovery of spontaneous vocal-fold movement during breathing in the HY group, whereas the M and PGA groups showed wholly no recoveries. The Sk-MSCs showed active engraftment confined to the damaged RLN portion, representing favorable prevention of cell diffusion on PGA, with an enhanced expression of nerve growth factor mRNAs. Axonal re-connection in the HY group was confirmed by histological serial sections. Immunohistochemical analysis revealed the differentiation of Sk-MSCs into Schwann cells and perineurial/endoneurial cells and axonal growth supportive of perineurium/endoneurium. The number of axons recovered was over 86%. These results showed that the stem cell and cytokine delivery system using hybrid transplantation of Sk-MSCs/PGA-felt is a potentially practical and useful approach for the recovery of transected RLN.

RECONSTITUTION OF EXPERIMENTAL NEUROGENIC BLADDER DYSFUNCTION USING SKELETAL MUSCLE-DERIVED MULTIPOTENT STEM CELLS

2009 ◽  
Vol 181 (4S) ◽  
pp. 45-46
Author(s):  
Masahiro Nitta ◽  
Tetsuro Tamaki ◽  
Akio Hoshi ◽  
Kayoko Tono ◽  
Maki Masuda ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Neurogenic Bladder ◽  
Bladder Dysfunction ◽  
Neurogenic Bladder Dysfunction ◽  
Multipotent Stem Cells

Reconstruction of Radical Prostatectomy-Induced Urethral Damage Using Skeletal Muscle-Derived Multipotent Stem Cells

2008 ◽  
Vol 85 (11) ◽  
pp. 1617-1624 ◽  
Author(s):  
Akio Hoshi ◽  
Tetsuro Tamaki ◽  
Kayoko Tono ◽  
Yoshinori Okada ◽  
Akira Akatsuka ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Radical Prostatectomy ◽  
Multipotent Stem Cells

1329: Reconstitution of Urethral Sphincter and Neurovascular Bundle Using Skeletal Muscle-Derived Multipotent Stem Cells

2007 ◽  
Vol 177 (4S) ◽  
pp. 438-438
Author(s):  
Akio Hoshi ◽  
Tetsuro Tamaki ◽  
Yoshinori Okada ◽  
Kayoko Tono ◽  
Masanori Shima ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Neurovascular Bundle ◽  
Urethral Sphincter ◽  
Multipotent Stem Cells

scholarly journals Intramuscular injection of mesenchymal stem cells activates anabolic and catabolic systems in mouse skeletal muscle

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Junya Takegaki ◽  
Kohei Sase ◽  
Yusuke Kono ◽  
Daiki Nakano ◽  
Takuya Fujita ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intramuscular Injection ◽  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Related Factors ◽  
Multipotent Stem Cells ◽  
Protein Ubiquitination ◽  
Mtorc1 Signaling

AbstractSkeletal muscle mass is critical for good quality of life. Mesenchymal stem cells (MSCs) are multipotent stem cells distributed across various tissues. They are characterized by the capacity to secrete growth factors and differentiate into skeletal muscle cells. These capabilities suggest that MSCs might be beneficial for muscle growth. Nevertheless, little is known regarding the effects on muscle protein anabolic and catabolic systems of intramuscular injection of MSCs into skeletal muscle. Therefore, in the present study, we measured changes in mechanistic target of rapamycin complex 1 (mTORC1) signaling, the ubiquitin–proteasome system, and autophagy-lysosome system-related factors after a single intramuscular injection of MSCs with green fluorescence protein (GFP) into mouse muscles. The intramuscularly-injected MSCs were retained in the gastrocnemius muscle for 7 days after the injection, indicated by detection of GFP and expression of platelet-derived growth factor receptor-alpha. The injection of MSCs increased the expression of satellite cell-related genes, activated mTORC1 signaling and muscle protein synthesis, and increased protein ubiquitination and autophagosome formation (indicated by the expression of microtubule-associated protein 1 light chain 3-II). These results suggest that the intramuscular injection of MSCs activated muscle anabolic and catabolic systems and accelerated muscle protein turnover.

Mesenchymal Stem Cells for Regenerative Medicine for Duchenne Muscular Dystrophy

2020 ◽  
Author(s):  
Ahmed Elhussieny ◽  
Ken’ichiro Nogami ◽  
Fusako Sakai-Takemura ◽  
Yusuke Maruyama ◽  
AbdElraouf Omar Abdelbakey ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Regenerative Medicine ◽  
Therapeutic Potential ◽  
Dystrophic Muscle ◽  
Multipotent Stem Cells ◽  
Pharmacological Target

Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated from both foetal and adult tissues. Several groups demonstrated that transplantation of MSCs promoted the regeneration of skeletal muscle and ameliorated muscular dystrophy in animal models. Mesenchymal stem cells in skeletal muscle, also known as fibro-adipogenic progenitors (FAPs), are essential for the maintenance of skeletal muscle. Importantly, they contribute to fibrosis and fat accumulation in dystrophic muscle. Therefore, MSCs in muscle are a pharmacological target for the treatment of muscular dystrophies. In this chapter, we briefly update the knowledge on mesenchymal stem/progenitor cells and discuss their therapeutic potential as a regenerative medicine treatment of Duchenne muscular dystrophy.

Reconstitution of Experimental Neurogenic Bladder Dysfunction Using Skeletal Muscle-Derived Multipotent Stem Cells

2010 ◽  
Vol 89 (9) ◽  
pp. 1043-1049 ◽  
Author(s):  
Masahiro Nitta ◽  
Tetsuro Tamaki ◽  
Kayoko Tono ◽  
Yoshinori Okada ◽  
Maki Masuda ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Neurogenic Bladder ◽  
Bladder Dysfunction ◽  
Neurogenic Bladder Dysfunction ◽  
Multipotent Stem Cells

scholarly journals Nerve Stimulator-guided Injection of Autologous Stem Cells Near the Equine Left Recurrent Laryngeal Nerve

10.3791/58023 ◽  
2018 ◽  
Author(s):  
Charlotte Sandersen ◽  
Justine Ceusters ◽  
Alexia Fourez ◽  
Irene Tosi ◽  
Helene Graide ◽  
...  
Keyword(s):  
Stem Cells ◽  
Recurrent Laryngeal Nerve ◽  
Laryngeal Nerve ◽  
Nerve Stimulator ◽  
Left Recurrent Laryngeal Nerve
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