scholarly journals Enhanced Expression but Decreased Specific Activity of Matrix Metalloproteinase 10 (MMP-10) in Comparison with Matrix Metalloproteinase 3 (MMP-3) in Human Urinary Bladder Carcinoma

2021 ◽  
Vol 10 (16) ◽  
pp. 3683
Author(s):  
Jacek Kudelski ◽  
Grzegorz Młynarczyk ◽  
Monika Gudowska-Sawczuk ◽  
Barbara Mroczko ◽  
Barbara Darewicz ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Matrix Metalloproteinases ◽  
Urinary Bladder ◽  
Matrix Metalloproteinase ◽  
Bladder Carcinoma ◽  
Urinary Bladder Cancer ◽  
Urinary Bladder Carcinoma ◽  
Low Grade ◽  
High Grade ◽  
Human Urinary Bladder

Human urinary bladder cancer is a huge worldwide oncological problem causing many deaths every year. The degradation of extracellular matrix (ECM) induced by molecules such as matrix metalloproteinases (MMPs) is one of the main factors influencing the process of metastasis origination. The MMP expression is tied to tumor aggressiveness, stage, and patient prognosis. The cleavage of constituent proteins is initiated and prolonged by matrix metalloproteinases, such as MMP-3 and MMP-10. The aim of this study was to evaluate the expression and activity of both MMPs in human urinary bladder cancer occurring at various stages of the disease. Tissue samples from patients with urinary bladder cancer were analyzed. Samples were collected from patients with a low- and high-grade cancer. Control tissue was collected from the site opposite to the tumor. DNA content, MMPs content, and activity of MMP-3 and MMP-10 were measured using ELISA and Western blot techniques. MMP-3 and MMP-10 occur in high molecular complexes in human urinary bladder in healthy and cancerous tissues. Particularly, in high-grade tumors, the content of MMP-10 prevails over MMP-3. The actual and specific activities vary in both grades of urinary bladder cancer; however, the highest activity for MMP-3 and MMP-10 was found in low-grade tissues. In conclusion, MMP-10 had a higher content, but a lower activity in all investigated tissues compared to MMP-3. Generally, obtained results demonstrated a contrary participation of MMP-3 and MMP-10 in ECM remodeling what may be crucial in the pathogenesis of human urinary bladder carcinoma.

Utility of first order MRI-Texture analysis parameters in the prediction of histologic grade and muscle invasion in urinary bladder cancer: a preliminary study

2021 ◽  
pp. 20201114
Author(s):  
Abdul Razik ◽  
Chandan J Das ◽  
Raju Sharma ◽  
Sundeep Malla ◽  
Sanjay Sharma ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urinary Bladder ◽  
Texture Analysis ◽  
Urinary Bladder Cancer ◽  
Histologic Grade ◽  
Low Grade ◽  
High Grade ◽  
First Order ◽  
Muscle Invasive ◽  
Muscle Invasion

Objective: To explore the utility of first-order MRI-texture analysis (TA) parameters in predicting histologic grade and muscle invasion in urinary bladder cancer (UBC). Methods: After ethical clearance, 40 patients with UBC, who were imaged on a 3.0-Tesla scanner, were retrospectively included. Using the TexRADTM platform, two readers placed freehand ROI on the sections demonstrating the largest dimension of the tumor, evaluating only one tumor per patient. Interobserver reproducibility was assessed using the intraclass correlation coefficient (ICC). Mann–Whitney U test and ROC curve analysis were used to identify statistical significance and select parameters with high class separation capacity (AUC >0.8), respectively. Pearson’s test was used to identify redundancy in the results. Results: All texture parameters showed excellent ICC. The best parameters in differentiating high and low-grade tumors were mean/ mean of positive pixels (MPP) at SSF 0 (AUC: 0.897) and kurtosis at SSF 5 (AUC: 0.828) on the ADC images. In differentiating muscle invasive from non-muscle invasive tumors, mean/ MPP at SSF 0 on the ADC images showed AUC >0.8; however, this finding resulted from the confounding effect of high-grade histology on the ADC values of muscle invasive tumors. Conclusion: MRI-TA generated few parameters which were reproducible and useful in predicting histologic grade. No independent parameters predicted muscle invasion. Advances in knowledge: There is lacuna in the literature concerning the role of MRI-TA in the prediction of histologic grade and muscle invasion in UBC. Our study generated a few first-order parameters which were useful in predicting high-grade histology.

scholarly journals Urinary Bladder Carcinoma: Impact of Smoking, Age and its Clinico-Pathological Spectrum

2015 ◽  
Vol 11 (4) ◽  
pp. 292-295 ◽  
Author(s):  
HN Joshi ◽  
R Makaju ◽  
A Karmacharya ◽  
RM Kamracharya ◽  
B Shrestha ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Occupational Exposure ◽  
Urinary Bladder ◽  
Bladder Carcinoma ◽  
Urinary Bladder Cancer ◽  
Aetiological Factor ◽  
Urinary Bladder Carcinoma ◽  
Chemical Carcinogen ◽  
History Of ◽  
The Impact

Background Urinary bladder carcinoma is common urological malignancy. Although epidemiological evidence favors role of occupational exposure to chemical carcinogen as the aetiological factor of bladder carcinoma, many cases arise with no obvious occupational exposure to chemical carcinogen. Tobacco and cigarette smoking is common in both rural and urban areas of Nepal.Objective The objective of this study was to determine the impact of smoking and age in urinary bladder carcinoma with related clinicopathological correlations.Method A total of 56 (44 males and 12 females) cases of urinary bladder cancer treated at Dhulikhel Hospital, Kathmandu University Teaching Hospital during time period of January 2004 to December 2013 were included in the study. Data of patients with Urinary bladder cancer were obtained from hospital records and evaluated for age, sex, history of smoking, clinical presentations, cystoscopic findings and histopathological characteristics.Results Out of 56 cases, 51 (91.1%) of the patients had hematuria. History of smoking was found in 44 patients. Smoking was found much higher in males (88%) than females (41.66%). Transitional cell carcinoma (TCC) was the most common histological variety, which was seen in 51 (91.07%) patients. The significant impact of smoking was found in terms of grade of TCC.Conclusion The incidence of bladder carcinoma is higher in male and TCC is the most common variety of Urinary bladder malignancy. History of smoking correlated with grade.Kathmandu Univ Med J 2013; 11(4): 292-295

Evaluation of Urine Tumor-Associated Trypsin Inhibitor, CYFRA 21-1, and Urinary Bladder Cancer Antigen for Detection of High-Grade Bladder Carcinoma

Urology ◽  
2008 ◽  
Vol 72 (5) ◽  
pp. 1159-1163 ◽  
Author(s):  
Ioannis Gkialas ◽  
Georgios Papadopoulos ◽  
Lydia Iordanidou ◽  
Georgios Stathouros ◽  
Chara Tzavara ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urinary Bladder ◽  
Trypsin Inhibitor ◽  
Bladder Carcinoma ◽  
Urinary Bladder Cancer ◽  
High Grade ◽  
Cancer Antigen
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