scholarly journals Melanoma Inhibitory Activity and Melanoma Inhibitory Activity 2 as Novel Immunohistochemical Markers of Oral Epithelial Dysplasia

2021 ◽  
Vol 10 (16) ◽  
pp. 3661
Author(s):  
Ryoko Kawai ◽  
Yoshihiko Sugita ◽  
Toshikatsu Suzumura ◽  
Takehiro Hattori ◽  
Waka Yoshida ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Inhibitory Activity ◽  
Epithelial Dysplasia ◽  
Low Grade ◽  
Oral Potentially Malignant Disorders ◽  
Cytoplasmic Expression ◽  
Oral Epithelial Dysplasia ◽  
Immunohistochemical Markers ◽  
Melanoma Inhibitory Activity ◽  
Oral Epithelial

Oral potentially malignant disorders are associated with the development of oral squamous cell carcinoma (OSCC). Most OSCCs are diagnosed via histopathology as oral epithelial dysplasia (OED), but the histologic diagnostic criteria remain non-uniform. Accordingly, the establishment of a diagnostic marker to assist in diagnosis could contribute towards cancer prevention. Melanoma inhibitory activity (MIA) and MIA2 are involved in tumor growth, invasion, and lymph node metastasis in various malignancies. The purpose of this study was to clarify the usefulness of MIA and MIA2 as diagnostic markers of oral mucosal lesions. The expression of MIA and MIA2 was analyzed immunohistochemically in 100 specimens (10 specimens with normal oral mucosa (NOM) and 30 specimens each with low-grade epithelial dysplasia (LED), high-grade epithelial dysplasia (HED), and OSCC). Immunohistochemical results were evaluated based on the Allred scoring system. Cytoplasmic expression of MIA and MIA2 increased in the order of LED, HED, and OSCC. All NOM specimens were negative for cytoplasmic expression. Significant differences were observed between the groups (NOM vs. HED, p < 0.05, NOM vs. OSCC, p < 0.001). These results demonstrate that MIA and MIA2 are expressed in the oral mucosa within early neoplastic lesions and suggest that MIA and MIA2 are useful novel immunohistochemical markers for discriminating between normal tissue and OED.

scholarly journals Clinical Review and Management of Oral Potentially Malignant Disorders with Epithelial Dysplasia

2021 ◽  
Author(s):  
Mustafa Mohammed Abdulhussain ◽  
Ali Sami Muhsin
Keyword(s):  
Oral Mucosa ◽  
Precancerous Lesion ◽  
Oral Lesion ◽  
Epithelial Dysplasia ◽  
Premalignant Lesions ◽  
Oral Potentially Malignant Disorders ◽  
Oral Epithelial Dysplasia ◽  
Normal Oral Mucosa ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

Background: Oral potentially malignant disorders (OPMDs) comprise any disorders, tumors, in addition to any microscopic alterations that have a risk of malignant development of cancers of the mouth. When epithelial dysplasia is detected in an oral lesion, it is termed as a precancerous lesion. Finding: Several changes in the color or thickness of normal oral mucosa might be detected during the clinical diagnosis of the oral lesions. Leukoplakia of the oral cavity is a clinical name for one of the most predominant OPMDs of the oral mucosa. When comparing oral examination with naked eyes to planning to apply staining with special stain or using an image of optical fluorescence, the incidence of patients with oral epithelial dysplasia may rise, as well as the clearing of the lesion boundary. Increased size of more than 2cm2, the presence of colored regions with a red hue, the presence of lichenoid process characteristics, and severe epithelial dysplasia are all considered risk factors. One-third of premalignant lesions may progress to cancer, whereas the other two-thirds may stay stable or regress without progressing to malignancy. Conclusion: It is critical to research the patients' unique characteristics, which include psychological, genetic, dietary, and dental problems. When epithelial dysplasia is present in an oral lesion, it is termed a precancerous lesion. Oral potential malignant diseases with epithelial dysplasia may or may not develop into carcinoma and may or may not be recurrent.

scholarly journals Clinical Review and Management of Oral Potentially Malignant Disorders with Epithelial Dysplasia

2021 ◽  
Vol 4 (4) ◽  
Author(s):  
Mustafa Mohammed Abdulhussain ◽  
◽  
Ali Sami Muhsin
Keyword(s):  
Oral Mucosa ◽  
Precancerous Lesion ◽  
Oral Lesion ◽  
Epithelial Dysplasia ◽  
Premalignant Lesions ◽  
Oral Potentially Malignant Disorders ◽  
Oral Epithelial Dysplasia ◽  
Normal Oral Mucosa ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant

Background: Oral potentially malignant disorders (OPMDs) comprise any disorders, tumors, in addition to any microscopic alterations that have a risk of malignant development of cancers of the mouth. When epithelial dysplasia is detected in an oral lesion, it is termed as a precancerous lesion. Finding: Several changes in the color or thickness of normal oral mucosa might be detected during the clinical diagnosis of the oral lesions. Leukoplakia of the oral cavity is a clinical name for one of the most predominant OPMDs of the oral mucosa. When comparing oral examination with naked eyes to planning to apply staining with special stain or using an image of optical fluorescence, the incidence of patients with oral epithelial dysplasia may rise, as well as the clearing of the lesion boundary. Increased size of more than 2cm2, the presence of colored regions with a red hue, the presence of lichenoid process characteristics, and severe epithelial dysplasia are all considered risk factors. One-third of premalignant lesions may progress to cancer, whereas the other two-thirds may stay stable or regress without progressing to malignancy. Conclusion: It is critical to research the patients' unique characteristics, which include psychological, genetic, dietary, and dental problems. When epithelial dysplasia is present in an oral lesion, it is termed a precancerous lesion. Oral potential malignant diseases with epithelial dysplasia may or may not develop into carcinoma and may or may not be recurrent.

scholarly journals Combination of Cytopathology and DNA Ploidy Increases the Performance of Oral Epithelial Dysplasia Prediction in Oral Potentially Malignant Disorders

2018 ◽  
Vol 4 (Supplement 2) ◽  
pp. 69s-69s
Author(s):  
Z.B.A. Karim ◽  
T.G. Kallarakkal ◽  
R. Amtha ◽  
M.V. Guledgud ◽  
A. Telang ◽  
...  
Keyword(s):  
Negative Predictive Value ◽  
Predictive Value ◽  
Dna Ploidy ◽  
Epithelial Dysplasia ◽  
Youden Index ◽  
Oral Potentially Malignant Disorders ◽  
Oral Epithelial Dysplasia ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Oral Epithelial

Background: Grading of oral epithelial dysplasia (OED) by a pathologist is currently the key guide used for treatment planning of oral potentially malignant disorders (OPMDs). Conventional oral examination (COE) clinically detects OPMDs but may not predict their risk status to transform to cancer. Therefore, there is a need for a reliable test to predict OED in OPMDs. Aim: This study was conducted to evaluate COE, liquid based cytology (Cytopath) and DNA image cytometry (Ploidy) in predicting OED in OPMDs. Methods: A total of 179 patients from Malaysia, India and Indonesia underwent COE followed by brush biopsies and scalpel biopsies. Brush-biopsy samples were analyzed for cytopathology and DNA ploidy at Dental Faculty, University of Malaya. Histopathological findings of presence/absence of OED were used as the reference standard. Calculations for sensitivity (Sn), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV) and accuracy (A) were done for individual tools and in combinations. The Youden index (Sn+Sp-1) was used as a measure of overall performance. The relevant medical ethics committees of the different research locations approved the study. Results: For COE, the sensitivity (Sn) was high (100%) and the specificity (Sp) was low (5.9%), while both Cytopath and Ploidy showed a low sensitivity (Sn) (28.6% and 22.2%) and high specificity (Sp) (94.3% and 82.3%). All 3 tools individually have high negative predictive value (NPV) for predicting presence of OED (COE-100%, Cytopath-66.7%, Ploidy-78.5%). When combining outcomes from all 3 tools, the best performance indicated by Youden index (42.1) is which defines a positive case when both COE and Cytopath show abnormal. In general, using results from at least 2 tools had better Youden indices than using these tools individually. Conclusion: COE as a screening tool by virtue of its high Sn would be a suitable first level diagnostic test, while the Cytopath and the Ploidy individually with high Sp may be used as a second level test to predict presence of OED. Combining the COE with cytopathology would be the best combination for a high performance of the tools. Cytopathology (when performed by a trained cytologist) would allow for most of the false positives from the first level test to be correctly identified as true negative at the second level. Longitudinal data are needed to assess which of these may correctly identify the malignant potential of OPMDs. Acknowledgment: Grant: High Impact Research - Ministry of Higher Education (HIR-MOHE UM000025/C3)

scholarly journals Visual Analytics: A Method to Explore Natural Histories of Oral Epithelial Dysplasia

2021 ◽  
Vol 2 ◽  
Author(s):  
Stan Nowak ◽  
Miriam Rosin ◽  
Wolfgang Stuerzlinger ◽  
Lyn Bartram
Keyword(s):  
Visual Analytics ◽  
Clinical Presentation ◽  
Epithelial Dysplasia ◽  
Biological Processes ◽  
Oral Potentially Malignant Disorders ◽  
Oral Epithelial Dysplasia ◽  
Potentially Malignant ◽  
Oral Epithelial ◽  
Natural Histories

Risk assessment and follow-up of oral potentially malignant disorders in patients with mild or moderate oral epithelial dysplasia is an ongoing challenge for improved oral cancer prevention. Part of the challenge is a lack of understanding of how observable features of such dysplasia, gathered as data by clinicians during follow-up, relate to underlying biological processes driving progression. Current research is at an exploratory phase where the precise questions to ask are not known. While traditional statistical and the newer machine learning and artificial intelligence methods are effective in well-defined problem spaces with large datasets, these are not the circumstances we face currently. We argue that the field is in need of exploratory methods that can better integrate clinical and scientific knowledge into analysis to iteratively generate viable hypotheses. In this perspective, we propose that visual analytics presents a set of methods well-suited to these needs. We illustrate how visual analytics excels at generating viable research hypotheses by describing our experiences using visual analytics to explore temporal shifts in the clinical presentation of epithelial dysplasia. Visual analytics complements existing methods and fulfills a critical and at-present neglected need in the formative stages of inquiry we are facing.

Sign in / Sign up

Export Citation Format

Share Document