scholarly journals Susceptibility Status and Resistance Mechanisms in Permethrin-Selected, Laboratory Susceptible and Field-Collected Aedes aegypti from Malaysia

Insects ◽  
2018 ◽  
Vol 9 (2) ◽  
pp. 43 ◽  
Author(s):  
Rosilawati Rasli ◽  
Han Lee ◽  
Nazni Wasi Ahmad ◽  
Siti Fikri ◽  
Roziah Ali ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Resistance Mechanisms ◽  
Susceptibility Status

scholarly journals Risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of aedes aegypti (linnaeus) in some sites in Accra, Ghana

2016 ◽  
Vol 50 (3) ◽  
pp. 136-141
Author(s):  
Takashi Suzuki ◽  
Joseph H Osei ◽  
Sasaki Sasaki ◽  
Michelle Adimazoya ◽  
Maxwell Appawu ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Emerging Diseases ◽  
Research Network ◽  
Insecticide Susceptibility ◽  
Aedes Mosquitoes ◽  
Human Landing ◽  
Survey Results ◽  
The University ◽  
University Of Ghana ◽  
Susceptibility Status

Background: Dengue is one of the emerging diseases that can mostly only be controlled by vector control since there is no vaccine for the disease. Although, Dengue has not been reported in Ghana, movement of people from neighbouring countries where the disease has been reported can facilitate transmission of the disease. Objective: This study was carried on the University of Ghana campus to determine the risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of Ae. aegypti in some sites in Accra, Ghana. Design: Larval surveys were carried to inspect containers within households and estimate larval indices and adult Aedes mosquitoes were collected using human landing collection technique. WHO tube assays was used to assess the insecticide susceptibility status of Aedes mosquitoes. Results: Ae. aegypti were the most prevalent species, 75.5% and followed by Ae. vittatus, 23.9 %. Ae. albopictus and Ae. granti were in smaller numbers. Household index (HI), Breteau index (BI), and container index were calculated as 8.2%, 11.2% and 10.3% respectively with man-vector contact rate of 0.67 bites/man-hour estimated for the area. The mortalities recorded for Ae. aegypti from WHO tube assays was 88%, 94%, 80% and 99% for DDT (4%), deltamethrin (0.05%), lambdacyhalothrin (0.05%) and permethrin (0.75%) respectively.Conclusion: The survey results indicated that the density of Aedes mosquitoes was considered to be sufficient to promote an outbreak of viral haemorrhagic fevers on Legon Campus. Aedes mosquitoes were found to be resistant to DDT, deltamethrin and lamdacyhalothrin, but susceptible to permethrin. Funding: This study was supported in part by Japan Initiative for Global Research Network on Infectious Diseases(J-Grid).Keywords: Mosquitoes, Aedes aegypti, insecticide, risk, VHF transmission, Ghana

scholarly journals Uji resistensi nyamuk aedes aegypti terhadap sipermetrin 0,05% dipelabuhan Sungai Duku dan Bandara Sultan Syarif Kasim II Pekanbaru

2021 ◽  
Vol 1 (1) ◽  
pp. 16-21
Author(s):  
Dedes Handayani ◽  
Zuhirman Zuhirman ◽  
Ridwan Manda Putra
Keyword(s):  
Aedes Aegypti ◽  
Cross Sectional ◽  
Vector Borne Disease ◽  
Port Area ◽  
Independent Test ◽  
Vector Borne ◽  
Cross Sectional Design ◽  
Susceptibility Status

Control of use of chemical insecticides is one way to reduce the vector borne disease dengue hemmorrhagic fever (DHF)which are caused by the mosquito Aedes aegypti. This study aims to determine the susceptibility status of Aedes aegypti to cypermethrin insecticide, the differences susceptibility status in the Duku River Port and Sultan Syarif Kasim Airport Pekanbaru.This study was an descriptif study with cross-sectional design. Egg and Larva Aedes aegypti mosquito sample collected from ovitrap and larva-place from Duku River Port area and Sultan Syarif Kasim II Airport. Test of susceptibility to cypermethrin 0.05 % was done using impregnated paper refers to the WHO method, T independent test to considaret susceptibility status different both area.The result showed that Ae. aegypti in Duku River Port area and Sultan Syarif Kasim II airport areas have been toleran cypermethrin 0.05%.There is no difference in the susceptibility of cypermethrin 0,05% to the population of Aedes aegypti in Duku River Port and Sultan Syarif Kasim II Airport.

scholarly journals VULNERABILITY TEST OF Aedes aegypti LARVA AGAINST TEMEFOS IN WAY KANDIS, BANDAR LAMPUNG

2020 ◽  
Vol 7 (2) ◽  
pp. 73-78
Author(s):  
Nurul Hanifah ◽  
EMANTIS ROSA ◽  
Endah Seytaningrum
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Incidence Rate ◽  
Dengue Fever ◽  
Susceptibility Status

Aedes aegypti is a major vector of Dengue Fever (DHF) that is spread through mosquito bites. However, Bandar Lampung ranked 3rd out of 15 districts in Lampung Province with an IR (Incidence Rate) of 59.43. One way to reduce dengue cases is vector control. Temefos is commonly used as a chemical vector's control and it is possible can cause resistance to larvae. The information about Aedes Aegypty has not been widely known about larval of Aedes aegypty's susceptibility towards Temefos in Lampung Province. Therefore, this study aims to determine the susceptibility status of Aedes aegypti larvae towards Temefos in Way Kandis Village, Tanjung Senang Regency, Bandar Lampung. This research was conducted in December 2018 - February 2019. This study uses five different temefos concentrations, i.e ;0 mg / L concentration as control, 0.005 mg / L, 0.01 mg / L, 0.02 mg / L, and 0 , Each 03 mg / L was repeated in 4 times. Observations were made by calculating the number of larvae that fainted, died, and lived. The results show that the susceptibility of Aedes aegypti towards Temefos in Way Kandis Village, Tanjung Senang Regency, Bandar Lampung City, is categorized as susceptible in the concentration range of 0.005 - 0.03 mg / L.

scholarly journals Transcriptomic and proteomic analysis of pyrethroid resistance in the CKR strain of Aedes aegypti

2021 ◽  
Vol 15 (11) ◽  
pp. e0009871
Author(s):  
Haina Sun ◽  
Robert W. Mertz ◽  
Letícia B. Smith ◽  
Jeffrey G. Scott
Keyword(s):  
Aedes Aegypti ◽  
Proteomic Analysis ◽  
Molecular Basis ◽  
Pyrethroid Resistance ◽  
Resistance Mechanisms ◽  
Chromosome 1 ◽  
Cytochrome P450 Monooxygenases ◽  
Rna Seq ◽  
Proteomics Data ◽  
Pyrethroid Insecticides

Aedes aegypti is an important vector of human viral diseases. This mosquito is distributed globally and thrives in urban environments, making it a serious risk to human health. Pyrethroid insecticides have been the mainstay for control of adult A. aegypti for decades, but resistance has evolved, making control problematic in some areas. One major mechanism of pyrethroid resistance is detoxification by cytochrome P450 monooxygenases (CYPs), commonly associated with the overexpression of one or more CYPs. Unfortunately, the molecular basis underlying this mechanism remains unknown. We used a combination of RNA-seq and proteomic analysis to evaluate the molecular basis of pyrethroid resistance in the highly resistant CKR strain of A. aegypti. The CKR strain has the resistance mechanisms from the well-studied Singapore (SP) strain introgressed into the susceptible Rockefeller (ROCK) strain genome. The RNA-seq and proteomics data were complimentary; each offering insights that the other technique did not provide. However, transcriptomic results did not quantitatively mirror results of the proteomics. There were 10 CYPs which had increased expression of both transcripts and proteins. These CYPs appeared to be largely trans-regulated, except for some CYPs for which we could not rule out gene duplication. We identified 65 genes and lncRNAs as potentially being responsible for elevating the expression of CYPs in CKR. Resistance was associated with multiple loci on chromosome 1 and at least one locus on chromosome 3. We also identified five CYPs that were overexpressed only as proteins, suggesting that stabilization of CYP proteins could be a mechanism of resistance. Future studies to increase the resolution of the resistance loci, and to examine the candidate genes and lncRNAs identified here will greatly enhance our understanding of CYP-mediated resistance in A. aegypti.

Pyrethroids Use: Threats on Metabolic-Mediated Resistance Mechanisms in the Primary Dengue Vector Aedes aegypti (Diptera: Culicidae)

2019 ◽  
Vol 56 (3) ◽  
pp. 811-816 ◽  
Author(s):  
Zheng Hua Amelia-Yap ◽  
Mohd Sofian-Azirun ◽  
Chee Dhang Chen ◽  
I Wayan Suana ◽  
Koon Weng Lau ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Resistance Mechanisms ◽  
Dengue Vector

scholarly journals Metode Monitoring Resistensi Populasi Aedes aegypti Dengan Penetapan Konsentrasi Diagnostik

2019 ◽  
Vol 20 (2) ◽  
pp. 105
Author(s):  
Vicka Kusuma Sari ◽  
Udi Tarwotjo ◽  
Mochamad Hadi
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Monitoring Method ◽  
Synthetic Insecticide ◽  
Validation Test ◽  
And Mathematics ◽  
Susceptibility Status ◽  
Aegypti Population ◽  
Main Factor

Aedes aegypti is the main vector of dengue fever disease. The most effective way to prevent dengue fever is cutting the spreading chain of dengue fever by controlling the vector with using conventional chemical insecticides. The used of insecticide intensively has became the main factor of resistance in Tembalang, then monitoring is needed to determine the change of A. aegypti susceptibility status against the insecticide. The aim of this research is to determine the sensitivity of A. aegypti population from five locations in Tembalang against pyrethroid synthetic insecticide, and to determine the validity of the diagnostic concentration as a method of monitoring resistance A. aegypti population in Tembalang. This research was conducted in Laboratory of Ecology and Biosystematics Department of Biology Faculty of Science and Mathematics, Diponegoro University Semarang. The procedure of this research were: larvae collection (A. aegypti), insect breeding test, sensitivity test including bioassay, determination of diagnostic concentration and validation. The result showed that the susceptibility level of five population was not significantly different since all populations are still susceptible, with the LC50 range between 0,0031-0,0043% and FR range between 1-1,39 (<4). The result of validation test of diagnostic concentration was 0,0038%, valid as a monitoring method of the resistance of A. aegypti population against pyrethroid synthetic insecticide in Tembalang because c2 value of the five population was lower than the c2 table (df = 1; α = 0,05) = 3,84. Keywords : A. aegypti, monitoring resistance, LC50, diagnostic concentration..

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